Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NSFL1 cofactor p47

Gene

Nsfl1c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER).4 Publications

GO - Molecular functioni

  • ATPase binding Source: RGD
  • lipid binding Source: UniProtKB-KW
  • ubiquitin binding Source: BHF-UCL

GO - Biological processi

  • Golgi organization Source: RGD
  • membrane fusion Source: RGD
  • nuclear envelope reassembly Source: GO_Central
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NSFL1 cofactor p47
Alternative name(s):
XY body-associated protein XY40
p97 cofactor p47
Gene namesi
Name:Nsfl1c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi619952. Nsfl1c.

Subcellular locationi

  • Nucleus
  • Golgi apparatusGolgi stack
  • Chromosome

  • Note: Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks.

GO - Cellular componenti

  • chromosome Source: UniProtKB-SubCell
  • cytosol Source: ParkinsonsUK-UCL
  • Golgi stack Source: UniProtKB-SubCell
  • intermediate filament cytoskeleton Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: GO_Central
  • plasma membrane Source: Ensembl
  • VCP-NSFL1C complex Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Golgi apparatus, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41F → A: Reduces ubiquitin binding and Golgi reassembly. 1 Publication1
Mutagenesisi57T → A: No effect on phosphorylation. 1 Publication1
Mutagenesisi112K → T: Strongly reduces nuclear location. 1 Publication1
Mutagenesisi114S → A: No effect on phosphorylation. 1 Publication1
Mutagenesisi140S → A: Abolishes phosphorylation by CDK1. 1 Publication1
Mutagenesisi140S → D: Strongly reduces binding to Golgi membranes. 1 Publication1
Mutagenesisi173R → T: Strongly reduces nuclear location. 1 Publication1
Mutagenesisi272S → A: No effect on phosphorylation. 1 Publication1
Mutagenesisi301R → A: Reduced interaction with VCP. 1 Publication1
Mutagenesisi342 – 345TFPN → AG: Strongly reduced interaction with VCP. 1 Publication4
Mutagenesisi343F → S: Reduced interaction with VCP. 1 Publication1
Mutagenesisi345N → A: Reduced interaction with VCP. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002109901 – 370NSFL1 cofactor p47Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei74PhosphoserineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Modified residuei114PhosphoserineCombined sources1
Modified residuei140Phosphoserine; by CDK11 Publication1
Modified residuei167PhosphotyrosineBy similarity1
Modified residuei176PhosphoserineCombined sources1
Modified residuei192PhosphoserineBy similarity1
Modified residuei272PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35987.
PRIDEiO35987.

PTM databases

iPTMnetiO35987.
PhosphoSitePlusiO35987.

Expressioni

Tissue specificityi

Highly expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis.3 Publications

Developmental stagei

Highly expressed in pachytene spermatocytes during spermatogenesis.

Gene expression databases

BgeeiENSRNOG00000008604.
ExpressionAtlasiO35987. baseline and differential.
GenevisibleiO35987. RN.

Interactioni

Subunit structurei

Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VcpP4646212EBI-1993760,EBI-399011
VcpQ018538EBI-1993760,EBI-80597From a different organism.

GO - Molecular functioni

  • ATPase binding Source: RGD
  • ubiquitin binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi249844. 4 interactors.
IntActiO35987. 18 interactors.
MINTiMINT-1954288.
STRINGi10116.ENSRNOP00000011654.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 15Combined sources12
Helixi20 – 29Combined sources10
Helixi35 – 43Combined sources9
Beta strandi181 – 187Combined sources7
Beta strandi190 – 192Combined sources3
Beta strandi198 – 200Combined sources3
Helixi206 – 215Combined sources10
Helixi221 – 224Combined sources4
Beta strandi227 – 229Combined sources3
Beta strandi232 – 237Combined sources6
Turni239 – 241Combined sources3
Helixi275 – 286Combined sources12
Beta strandi298 – 302Combined sources5
Turni303 – 306Combined sources4
Beta strandi307 – 311Combined sources5
Beta strandi314 – 316Combined sources3
Helixi318 – 328Combined sources11
Helixi331 – 333Combined sources3
Beta strandi337 – 341Combined sources5
Turni342 – 345Combined sources4
Helixi355 – 358Combined sources4
Beta strandi364 – 369Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I42NMR-A282-370[»]
1JRUNMR-A282-370[»]
1S3SX-ray2.90G/H/I244-370[»]
1V92NMR-A1-46[»]
1VAZNMR-A171-246[»]
ProteinModelPortaliO35987.
SMRiO35987.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35987.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 244SEPPROSITE-ProRule annotationAdd BLAST66
Domaini291 – 368UBXPROSITE-ProRule annotationAdd BLAST78

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi109 – 115Nuclear localization signal7
Motifi172 – 175Nuclear localization signal4

Sequence similaritiesi

Belongs to the NSFL1C family.Curated
Contains 1 SEP domain.PROSITE-ProRule annotation
Contains 1 UBX domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2086. Eukaryota.
ENOG410YGXU. LUCA.
GeneTreeiENSGT00520000055567.
HOGENOMiHOG000159961.
HOVERGENiHBG054517.
InParanoidiO35987.
KOiK14012.
OMAiQTIGDVY.
OrthoDBiEOG091G0QFZ.
PhylomeDBiO35987.

Family and domain databases

InterProiIPR012989. SEP_domain.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF102848. SSF102848. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEERQDALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI
60 70 80 90 100
VTISQATPSS VSRGTAPSDN RVTSFRDLIH DQDEEEEEEE GQRFYAGGSE
110 120 130 140 150
RSGQQIVGPP RKKSPNELVD DLFKGAKEHG AVAVERVTKS PGETSKPRPF
160 170 180 190 200
AGGGYRLGAA PEEESAYVAG ERRRHSGQDV HVVLKLWKTG FSLDNGDLRS
210 220 230 240 250
YQDPSNAQFL ESIRRGEVPA ELRRLAHGGQ VNLDMEDHRD EDFVKPKGAF
260 270 280 290 300
KAFTGEGQKL GSTAPQVLNT SSPAQQAENE AKASSSILIN EAEPTTNIQI
310 320 330 340 350
RLADGGRLVQ KFNHSHRISD IRLFIVDARP AMAATSFVLM TTFPNKELAD
360 370
ENQTLKEANL LNAVIVQRLT
Length:370
Mass (Da):40,680
Last modified:January 1, 1998 - v1
Checksum:i60C81402E5C58134
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10769 mRNA. Translation: CAA71742.1.
AB002086 mRNA. Translation: BAA21659.1.
BC072464 mRNA. Translation: AAH72464.1.
RefSeqiNP_114187.1. NM_031981.2.
UniGeneiRn.2771.

Genome annotation databases

EnsembliENSRNOT00000011654; ENSRNOP00000011654; ENSRNOG00000008604.
GeneIDi83809.
KEGGirno:83809.
UCSCiRGD:619952. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10769 mRNA. Translation: CAA71742.1.
AB002086 mRNA. Translation: BAA21659.1.
BC072464 mRNA. Translation: AAH72464.1.
RefSeqiNP_114187.1. NM_031981.2.
UniGeneiRn.2771.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I42NMR-A282-370[»]
1JRUNMR-A282-370[»]
1S3SX-ray2.90G/H/I244-370[»]
1V92NMR-A1-46[»]
1VAZNMR-A171-246[»]
ProteinModelPortaliO35987.
SMRiO35987.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249844. 4 interactors.
IntActiO35987. 18 interactors.
MINTiMINT-1954288.
STRINGi10116.ENSRNOP00000011654.

PTM databases

iPTMnetiO35987.
PhosphoSitePlusiO35987.

Proteomic databases

PaxDbiO35987.
PRIDEiO35987.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011654; ENSRNOP00000011654; ENSRNOG00000008604.
GeneIDi83809.
KEGGirno:83809.
UCSCiRGD:619952. rat.

Organism-specific databases

CTDi55968.
RGDi619952. Nsfl1c.

Phylogenomic databases

eggNOGiKOG2086. Eukaryota.
ENOG410YGXU. LUCA.
GeneTreeiENSGT00520000055567.
HOGENOMiHOG000159961.
HOVERGENiHBG054517.
InParanoidiO35987.
KOiK14012.
OMAiQTIGDVY.
OrthoDBiEOG091G0QFZ.
PhylomeDBiO35987.

Miscellaneous databases

EvolutionaryTraceiO35987.
PROiO35987.

Gene expression databases

BgeeiENSRNOG00000008604.
ExpressionAtlasiO35987. baseline and differential.
GenevisibleiO35987. RN.

Family and domain databases

InterProiIPR012989. SEP_domain.
IPR009060. UBA-like.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTiSM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF102848. SSF102848. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiPS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSF1C_RAT
AccessioniPrimary (citable) accession number: O35987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.