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Reviewed, UniProtKB/Swiss-Prot O35987 (NSF1C_RAT)

Last modified June 16, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    NSFL1 cofactor p47
Alternative name(s):
    p97 cofactor p47
    XY body-associated protein XY40
Gene names
Name: Nsfl1c
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Ref.2 Ref.7 Ref.9 Ref.11

Subunit structure

Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP.

Subcellular location

Nucleus. Golgi apparatusGolgi stack. Note: Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. Ref.2 Ref.5 Ref.13

Tissue specificity

Highly expressed in heart, brain, spleen, lung, liver, muscle, kidney and testis. Ref.2 Ref.5 Ref.1

Developmental stage

Highly expressed in pachytene spermatocytes during spermatogenesis.

Post-translational modification

Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis. Ref.13 Ref.14 Ref.15

Sequence similarities

Belongs to the NSFL1C family.

Contains 1 SEP domain.

Contains 1 UBX domain.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Nucleus
   LigandLipid-binding
   PTMPhosphoprotein
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processGolgi organization Ref.10

Inferred from physical interaction. Source: RGD

membrane fusion Ref.10

Inferred from direct assay. Source: RGD

   Cellular componentGolgi stack

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionlipid binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding Ref.2

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VcpP464622EBI-1993760,EBI-399011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370NSFL1 cofactor p47
PRO_0000210990

Regions

Domain179 – 24466SEP
Domain291 – 36878UBX
Motif109 – 1157Nuclear localization signal
Motif172 – 1754Nuclear localization signal

Amino acid modifications

Modified residue741Phosphoserine By similarity
Modified residue1141Phosphoserine Ref.14 Ref.15
Modified residue1401Phosphoserine; by CDC2
Modified residue1651Phosphoserine By similarity
Modified residue1671Phosphotyrosine By similarity
Modified residue1761Phosphoserine Ref.15
Modified residue2721Phosphoserine By similarity

Experimental info

Mutagenesis411F → A: Reduces ubiquitin binding and Golgi reassembly. Ref.11
Mutagenesis571T → A: No effect on phosphorylation. Ref.13
Mutagenesis1121K → T: Strongly reduces nuclear location. Ref.13
Mutagenesis1141S → A: No effect on phosphorylation. Ref.13
Mutagenesis1401S → A: Abolishes phosphorylation by CDC2. Ref.13
Mutagenesis1401S → D: Strongly reduces binding to Golgi membranes. Ref.13
Mutagenesis1731R → T: Strongly reduces nuclear location. Ref.13
Mutagenesis2721S → A: No effect on phosphorylation. Ref.13
Mutagenesis3011R → A: Reduced interaction with VCP. Ref.17
Mutagenesis342 – 3454TFPN → AG: Strongly reduced interaction with VCP. Ref.17
Mutagenesis3431F → S: Reduced interaction with VCP. Ref.17
Mutagenesis3451N → A: Reduced interaction with VCP. Ref.17

Secondary structure

........................................ 370
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O35987-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 60C81402E5C58134

FASTA37040,680
        10         20         30         40         50         60 
MAEERQDALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI VTISQATPSS 

        70         80         90        100        110        120 
VSRGTAPSDN RVTSFRDLIH DQDEEEEEEE GQRFYAGGSE RSGQQIVGPP RKKSPNELVD 

       130        140        150        160        170        180 
DLFKGAKEHG AVAVERVTKS PGETSKPRPF AGGGYRLGAA PEEESAYVAG ERRRHSGQDV 

       190        200        210        220        230        240 
HVVLKLWKTG FSLDNGDLRS YQDPSNAQFL ESIRRGEVPA ELRRLAHGGQ VNLDMEDHRD 

       250        260        270        280        290        300 
EDFVKPKGAF KAFTGEGQKL GSTAPQVLNT SSPAQQAENE AKASSSILIN EAEPTTNIQI 

       310        320        330        340        350        360 
RLADGGRLVQ KFNHSHRISD IRLFIVDARP AMAATSFVLM TTFPNKELAD ENQTLKEANL 

       370 
LNAVIVQRLT 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization and expression pattern of XY body-associated protein XY40 of the rat."
Alsheimer M., Imamichi Y., Heid H., Benavente R.
Chromosoma 106:308-314(1997) [PubMed: 9297509] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 200-209; 283-292 AND 323-328, TISSUE SPECIFICITY.
Strain: Wistar.
Tissue: Spermatocyte.
[2]"p47 is a cofactor for p97-mediated membrane fusion."
Kondo H., Rabouille C., Newman R., Levine T.P., Pappin D., Freemont P., Warren G.
Nature 388:75-78(1997) [PubMed: 9214505] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-22; 94-101; 157-172; 189-214; 260-282; 323-346 AND 357-368, MASS SPECTROMETRY, FUNCTION, INTERACTION WITH VCP, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[4]Lubec G., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 200-214 AND 283-301, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
[5]"Meiosis-specific protein selectively associated with sex chromosomes of rat pachytene spermatocytes."
Smith A., Benavente R.
Proc. Natl. Acad. Sci. U.S.A. 89:6938-6942(1992) [PubMed: 1495983] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[6]"Syntaxin 5 is a common component of the NSF- and p97-mediated reassembly pathways of Golgi cisternae from mitotic Golgi fragments in vitro."
Rabouille C., Kondo H., Newman R., Hui N., Freemont P., Warren G.
Cell 92:603-610(1998) [PubMed: 9506515] [Abstract]
Cited for: INTERACTION WITH STX5A.
[7]"The p47 co-factor regulates the ATPase activity of the membrane fusion protein, p97."
Meyer H.H., Kondo H., Warren G.
FEBS Lett. 437:255-257(1998) [PubMed: 9824302] [Abstract]
Cited for: FUNCTION.
[8]"A complex of mammalian ufd1 and npl4 links the AAA-ATPase, p97, to ubiquitin and nuclear transport pathways."
Meyer H.H., Shorter J.G., Seemann J., Pappin D., Warren G.
EMBO J. 19:2181-2192(2000) [PubMed: 10811609] [Abstract]
Cited for: INTERACTION WITH VCP.
[9]"Role of p97 and syntaxin 5 in the assembly of transitional endoplasmic reticulum."
Roy L., Bergeron J.J.M., Lavoie C., Hendriks R., Gushue J., Fazel A., Pelletier A., Morre D.J., Subramaniam V.N., Hong W., Paiement J.
Mol. Biol. Cell 11:2529-2542(2000) [PubMed: 10930451] [Abstract]
Cited for: FUNCTION.
[10]"Phospholipid species act as modulators in p97/p47-mediated fusion of Golgi membranes."
Pecheur E.-I., Martin I., Maier O., Bakowsky U., Ruysschaert J.-M., Hoekstra D.
Biochemistry 41:9813-9823(2002) [PubMed: 12146947] [Abstract]
Cited for: INTERACTION WITH MEMBRANES.
[11]"Direct binding of ubiquitin conjugates by the mammalian p97 adaptor complexes, p47 and Ufd1-Npl4."
Meyer H.H., Wang Y., Warren G.
EMBO J. 21:5645-5652(2002) [PubMed: 12411482] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF PHE-41.
[12]"VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo."
Uchiyama K., Jokitalo E., Kano F., Murata M., Zhang X., Canas B., Newman R., Rabouille C., Pappin D., Freemont P., Kondo H.
J. Cell Biol. 159:855-866(2002) [PubMed: 12473691] [Abstract]
Cited for: INTERACTION WITH VCIP135.
[13]"The localization and phosphorylation of p47 are important for Golgi disassembly-assembly during the cell cycle."
Uchiyama K., Jokitalo E., Lindman M., Jackman M., Kano F., Murata M., Zhang X., Kondo H.
J. Cell Biol. 161:1067-1079(2003) [PubMed: 12810701] [Abstract]
Cited for: PHOSPHORYLATION, MUTAGENESIS OF THR-57; LYS-112; SER-114; SER-140; ARG-173 AND SER-272, SUBCELLULAR LOCATION.
[14]"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS."
Moser K., White F.M.
J. Proteome Res. 5:98-104(2006) [PubMed: 16396499] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, MASS SPECTROMETRY.
Tissue: Liver.
[15]"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed: 16641100] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114 AND SER-176, MASS SPECTROMETRY.
Tissue: Kidney.
[16]"Solution structure and interaction surface of the C-terminal domain from p47: a major p97-cofactor involved in SNARE disassembly."
Yuan X., Shaw A., Zhang X., Kondo H., Lally J., Freemont P.S., Matthews S.
J. Mol. Biol. 311:255-263(2001) [PubMed: 11478859] [Abstract]
Cited for: STRUCTURE BY NMR OF 282-370, INTERACTION WITH VCP.
[17]"Structural basis of the interaction between the AAA ATPase p97/VCP and its adaptor protein p47."
Dreveny I., Kondo H., Uchiyama K., Shaw A., Zhang X., Freemont P.S.
EMBO J. 23:1030-1039(2004) [PubMed: 14988733] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 244-370, INTERACTION WITH VCP, MUTAGENESIS OF ARG-301; 342-THR--ASN-345; PHE-343 AND ASN-345.
[18]"Structure, dynamics and interactions of p47, a major adaptor of the AAA ATPase, p97."
Yuan X., Simpson P., McKeown C., Kondo H., Uchiyama K., Wallis R., Dreveny I., Keetch C., Zhang X., Robinson C., Freemont P., Matthews S.
EMBO J. 23:1463-1473(2004) [PubMed: 15029246] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-46 IN COMPLEX WITH UBQUITIN, STRUCTURE BY NMR OF 171-246, INTERACTION WITH VCP.

Cross-references

Sequence databases

Y10769 mRNA. Translation: CAA71742.1.
AB002086 mRNA. Translation: BAA21659.1.
BC072464 mRNA. Translation: AAH72464.1.
IPIIPI00214262.
RefSeqNP_114187.1.
UniGeneRn.2771

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1I42NMR-A282-370[»]
1JRUNMR-A282-370[»]
1S3SX-ray2.90G/H/I244-370[»]
1V92NMR-A1-46[»]
1VAZNMR-A171-246[»]
SMRO35987. Positions 171-270.
ModBaseSearch...

Protein-protein interaction databases

IntActO35987. 15 interactions.

PTM databases

PhosphoSiteO35987.

Genome annotation databases

EnsemblENSRNOG00000008604. Rattus norvegicus. [Contig view]
GeneID83809.
KEGGrno:83809.

Organism-specific databases

RGD619952. Nsfl1c.

Phylogenomic databases

HOVERGENO35987.

Gene expression databases

ArrayExpressO35987.
GermOnlineENSRNOG00000008604. Rattus norvegicus.

Family and domain databases

InterProIPR012989. SEP.
IPR001012. UBX.
[Graphical view]
PfamPF08059. SEP. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SMARTSM00553. SEP. 1 hit.
SM00166. UBX. 1 hit.
[Graphical view]
PROSITEPS51399. SEP. 1 hit.
PS50033. UBX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio616417.

Entry information

Entry nameNSF1C_RAT
AccessionPrimary (citable) accession number: O35987
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents