Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endonuclease III-like protein 1

Gene

Nthl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.UniRule annotation1 Publication

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationBy similarity

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei208Nucleophile; for N-glycosylase activityUniRule annotation1
Sitei227Important for catalytic activityUniRule annotation1
Metal bindingi278Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi285Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi288Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi294Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair, AP site formation Source: MGI
  • DNA repair Source: MGI
  • nucleotide-excision repair, DNA incision, 5'-to lesion Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase, Lyase
Biological processDNA damage, DNA repair
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.99.18 3474
ReactomeiR-MMU-110329 Cleavage of the damaged pyrimidine
R-MMU-110357 Displacement of DNA glycosylase by APEX1

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease III-like protein 1UniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
Alternative name(s):
Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Short name:
DNA glycosylase/AP lyaseUniRule annotation
Gene namesi
Name:Nthl1
Synonyms:Nth1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1313275 Nthl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19MitochondrionUniRule annotationAdd BLAST19
ChainiPRO_000010222820 – 300Endonuclease III-like protein 1Add BLAST281

Proteomic databases

MaxQBiO35980
PaxDbiO35980
PRIDEiO35980

PTM databases

iPTMnetiO35980
PhosphoSitePlusiO35980

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

BgeeiENSMUSG00000041429
CleanExiMM_NTHL1
GenevisibleiO35980 MM

Interactioni

Subunit structurei

Interacts with YBX1.UniRule annotation

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000047413

Structurei

3D structure databases

ProteinModelPortaliO35980
SMRiO35980
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 211HhHUniRule annotationAdd BLAST25

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1921 Eukaryota
COG0177 LUCA
GeneTreeiENSGT00510000047513
HOGENOMiHOG000252209
HOVERGENiHBG052675
InParanoidiO35980
KOiK10773
OMAiWQQFTHL
OrthoDBiEOG091G0IVB
TreeFamiTF314967

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_03183 Endonuclease_III_Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR030841 NTH1
PfamiView protein in Pfam
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSGVRMVTR SRSRATRIAS EGCREELAPR EAAAEGRKSH RPVRHPRRTQ
60 70 80 90 100
KTHVAYEAAN GEEGEDAEPL KVPVWEPQNW QQQLANIRIM RSKKDAPVDQ
110 120 130 140 150
LGAEHCYDAS ASPKVRRYQV LLSLMLSSQT KDQVTAGAMQ RLRARGLTVE
160 170 180 190 200
SILQTDDDTL GRLIYPVGFW RNKVKYIKQT TAILQQRYEG DIPASVAELV
210 220 230 240 250
ALPGVGPKMA HLAMAVAWGT ISGIAVDTHV HRIANRLRWT KKMTKTPEET
260 270 280 290 300
RKNLEEWLPR VLWSEVNGLL VGFGQQICLP VHPRCQACLN KALCPAAQDL
Length:300
Mass (Da):33,637
Last modified:April 16, 2014 - v2
Checksum:i09CAA1FD066F18EB
GO

Sequence cautioni

The sequence AK033701 differs from that shown. Reason: Frameshift at position 33.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB006812 mRNA Translation: BAA22080.1
AB009371 Genomic DNA Translation: BAA28846.1
AJ001617 Genomic DNA Translation: CAB65239.1
Y09688 mRNA Translation: CAA70866.1
AK033701 mRNA No translation available.
AC132367 Genomic DNA No translation available.
CCDSiCCDS28487.1
RefSeqiNP_032769.2, NM_008743.2
UniGeneiMm.148315

Genome annotation databases

EnsembliENSMUST00000047611; ENSMUSP00000047413; ENSMUSG00000041429
GeneIDi18207
KEGGimmu:18207
UCSCiuc008axi.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNTH_MOUSE
AccessioniPrimary (citable) accession number: O35980
Secondary accession number(s): E9QMW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: April 16, 2014
Last modified: May 23, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health