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Protein

T-cell ecto-ADP-ribosyltransferase 2

Gene

Art2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has both NAD+ glycohydrolase and ADP-ribosyltransferase activity.2 Publications

Catalytic activityi

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.2 Publications
NAD+ + H2O = ADP-D-ribose + nicotinamide.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981NADBy similarity
Binding sitei146 – 1461NADBy similarity
Binding sitei164 – 1641NADBy similarity
Binding sitei202 – 2021NADBy similarity
Active sitei216 – 2161By similarity

GO - Molecular functioni

  • hydrolase activity, acting on glycosyl bonds Source: UniProtKB
  • NAD(P)+-protein-arginine ADP-ribosyltransferase activity Source: UniProtKB
  • NAD+ nucleosidase activity Source: UniProtKB-EC

GO - Biological processi

  • NAD catabolic process Source: UniProtKB
  • peptidyl-arginine ADP-ribosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Ligandi

NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell ecto-ADP-ribosyltransferase 2 (EC:2.4.2.312 Publications)
Alternative name(s):
ADP-ribosyltransferase C2 and C3 toxin-like 2
Short name:
ARTC2
Mono(ADP-ribosyl)transferase 2B
NAD(+) glycohydrolase (EC:3.2.2.51 Publication)
T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2
T-cell differentiation marker Rt6 homolog 2
T-cell mono(ADP-ribosyl)transferase 2
Gene namesi
Name:Art2b
Synonyms:Rt6-2, Rt6.2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:107545. Art2b.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: UniProtKB
  • extrinsic component of plasma membrane Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi80 – 801S → C: Changes thiol-independent NADase activity to thiol-dependent; when associated with C-201. 1 Publication
Mutagenesisi201 – 2011F → C: Changes thiol-independent NADase activity to thiol-dependent; when associated with C-80. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 260240T-cell ecto-ADP-ribosyltransferase 2PRO_0000019321Add
BLAST
Propeptidei261 – 28929Removed in mature formBy similarityPRO_0000019322Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 246By similarity
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence analysis
Disulfide bondi141 ↔ 193By similarity
Glycosylationi249 – 2491N-linked (GlcNAc...)Sequence analysis
Lipidationi260 – 2601GPI-anchor amidated serineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO35975.
PRIDEiO35975.

PTM databases

iPTMnetiO35975.
PhosphoSiteiO35975.
SwissPalmiO35975.

Expressioni

Tissue specificityi

Expressed in spleen, intestine and thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000030651.
CleanExiMM_ART2B.
GenevisibleiO35975. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065658.

Structurei

3D structure databases

ProteinModelPortaliO35975.
SMRiO35975. Positions 24-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J95D. Eukaryota.
ENOG410ZKT4. LUCA.
GeneTreeiENSGT00530000062975.
HOGENOMiHOG000273888.
HOVERGENiHBG004464.
InParanoidiO35975.
KOiK19980.
OMAiICAYTAG.
OrthoDBiEOG091G0BSN.
TreeFamiTF335356.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKIFKFFL TWWLTQQVTG LAVPFMLDMA PNAFDDQYES CVEDMEKKAP
60 70 80 90 100
QLLQEDFNMN EELKLEWEKA EINWKEIKNS TSYPAGFHDF HGTALVAYTG
110 120 130 140 150
NLAIDFNRAV RDFKKSPDNF HYKAFHYYLT RAVQLLNDQG CSLVYRGTKV
160 170 180 190 200
MFEYTGKGSV RFGQFSSSSL TKRVALSSNF FSNHGTLFII RTCLGVNIKE
210 220 230 240 250
FSSFPREEEV LIPGYEVYHK VTAQNDNGYN EIFLDSPERK KSNFNCFYNG
260 270 280
SAQTVNIDFS ISGSRESCVS LFLVVLLGLL VQQLTLAEL
Length:289
Mass (Da):33,124
Last modified:June 13, 2012 - v2
Checksum:iBA3748FA37636051
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21T → P in CAA60948 (PubMed:8811076).Curated
Sequence conflicti2 – 21T → P in AAB71683 (PubMed:9300695).Curated
Sequence conflicti2 – 21T → P in AAB71684 (PubMed:9300695).Curated
Sequence conflicti4 – 41K → N in AAB71683 (PubMed:9300695).Curated
Sequence conflicti4 – 41K → N in AAB71684 (PubMed:9300695).Curated
Sequence conflicti5 – 51I → N in CAA60948 (PubMed:8811076).Curated
Sequence conflicti5 – 51I → N in AAB71683 (PubMed:9300695).Curated
Sequence conflicti5 – 51I → N in AAB71684 (PubMed:9300695).Curated
Sequence conflicti40 – 401S → G in CAA60948 (PubMed:8811076).Curated
Sequence conflicti115 – 1151K → I in CAA60948 (PubMed:8811076).Curated
Sequence conflicti231 – 2311E → Q in CAA60948 (PubMed:8811076).Curated
Sequence conflicti289 – 2891L → P in CAA60948 (PubMed:8811076).Curated
Sequence conflicti289 – 2891L → P in AAB71683 (PubMed:9300695).Curated
Sequence conflicti289 – 2891L → P in AAB71684 (PubMed:9300695).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87612 mRNA. Translation: CAA60948.1.
AF016463 mRNA. Translation: AAB71683.1.
AF016465 mRNA. Translation: AAB71684.1.
AC129609 Genomic DNA. No translation available.
CCDSiCCDS21512.1.
RefSeqiNP_064299.2. NM_019915.2.
XP_006507319.1. XM_006507256.2.
UniGeneiMm.57008.

Genome annotation databases

EnsembliENSMUST00000063920; ENSMUSP00000065658; ENSMUSG00000030651.
GeneIDi11872.
KEGGimmu:11872.
UCSCiuc009ioy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87612 mRNA. Translation: CAA60948.1.
AF016463 mRNA. Translation: AAB71683.1.
AF016465 mRNA. Translation: AAB71684.1.
AC129609 Genomic DNA. No translation available.
CCDSiCCDS21512.1.
RefSeqiNP_064299.2. NM_019915.2.
XP_006507319.1. XM_006507256.2.
UniGeneiMm.57008.

3D structure databases

ProteinModelPortaliO35975.
SMRiO35975. Positions 24-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065658.

PTM databases

iPTMnetiO35975.
PhosphoSiteiO35975.
SwissPalmiO35975.

Proteomic databases

PaxDbiO35975.
PRIDEiO35975.

Protocols and materials databases

DNASUi11872.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063920; ENSMUSP00000065658; ENSMUSG00000030651.
GeneIDi11872.
KEGGimmu:11872.
UCSCiuc009ioy.1. mouse.

Organism-specific databases

CTDi11872.
MGIiMGI:107545. Art2b.

Phylogenomic databases

eggNOGiENOG410J95D. Eukaryota.
ENOG410ZKT4. LUCA.
GeneTreeiENSGT00530000062975.
HOGENOMiHOG000273888.
HOVERGENiHBG004464.
InParanoidiO35975.
KOiK19980.
OMAiICAYTAG.
OrthoDBiEOG091G0BSN.
TreeFamiTF335356.

Miscellaneous databases

PROiO35975.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030651.
CleanExiMM_ART2B.
GenevisibleiO35975. MM.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAR2B_MOUSE
AccessioniPrimary (citable) accession number: O35975
Secondary accession number(s): F8VQK8, O35702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 13, 2012
Last modified: September 7, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.