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Protein

T-cell ecto-ADP-ribosyltransferase 2

Gene

Art2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has both NAD+ glycohydrolase and ADP-ribosyltransferase activity.2 Publications

Catalytic activityi

NAD+ + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine.2 Publications
NAD+ + H2O = ADP-D-ribose + nicotinamide.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98NADBy similarity1
Binding sitei146NADBy similarity1
Binding sitei164NADBy similarity1
Binding sitei202NADBy similarity1
Active sitei216By similarity1

GO - Molecular functioni

  • hydrolase activity, acting on glycosyl bonds Source: UniProtKB
  • NAD(P)+-protein-arginine ADP-ribosyltransferase activity Source: UniProtKB
  • NAD+ nucleosidase activity Source: UniProtKB-EC

GO - Biological processi

  • NAD catabolic process Source: UniProtKB
  • peptidyl-arginine ADP-ribosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Ligandi

NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell ecto-ADP-ribosyltransferase 2 (EC:2.4.2.312 Publications)
Alternative name(s):
ADP-ribosyltransferase C2 and C3 toxin-like 2
Short name:
ARTC2
Mono(ADP-ribosyl)transferase 2B
NAD(+) glycohydrolase (EC:3.2.2.51 Publication)
T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2
T-cell differentiation marker Rt6 homolog 2
T-cell mono(ADP-ribosyl)transferase 2
Gene namesi
Name:Art2b
Synonyms:Rt6-2, Rt6.2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:107545. Art2b.

Subcellular locationi

GO - Cellular componenti

  • anchored component of external side of plasma membrane Source: UniProtKB
  • extrinsic component of plasma membrane Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi80S → C: Changes thiol-independent NADase activity to thiol-dependent; when associated with C-201. 1 Publication1
Mutagenesisi201F → C: Changes thiol-independent NADase activity to thiol-dependent; when associated with C-80. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000001932121 – 260T-cell ecto-ADP-ribosyltransferase 2Add BLAST240
PropeptideiPRO_0000019322261 – 289Removed in mature formBy similarityAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 246By similarity
Glycosylationi79N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi141 ↔ 193By similarity
Glycosylationi249N-linked (GlcNAc...)Sequence analysis1
Lipidationi260GPI-anchor amidated serineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiO35975.
PRIDEiO35975.

PTM databases

iPTMnetiO35975.
PhosphoSitePlusiO35975.
SwissPalmiO35975.

Expressioni

Tissue specificityi

Expressed in spleen, intestine and thymus.1 Publication

Gene expression databases

BgeeiENSMUSG00000030651.
CleanExiMM_ART2B.
GenevisibleiO35975. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065658.

Structurei

3D structure databases

ProteinModelPortaliO35975.
SMRiO35975.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J95D. Eukaryota.
ENOG410ZKT4. LUCA.
GeneTreeiENSGT00530000062975.
HOGENOMiHOG000273888.
HOVERGENiHBG004464.
InParanoidiO35975.
KOiK19980.
OMAiICAYTAG.
OrthoDBiEOG091G0BSN.
TreeFamiTF335356.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKIFKFFL TWWLTQQVTG LAVPFMLDMA PNAFDDQYES CVEDMEKKAP
60 70 80 90 100
QLLQEDFNMN EELKLEWEKA EINWKEIKNS TSYPAGFHDF HGTALVAYTG
110 120 130 140 150
NLAIDFNRAV RDFKKSPDNF HYKAFHYYLT RAVQLLNDQG CSLVYRGTKV
160 170 180 190 200
MFEYTGKGSV RFGQFSSSSL TKRVALSSNF FSNHGTLFII RTCLGVNIKE
210 220 230 240 250
FSSFPREEEV LIPGYEVYHK VTAQNDNGYN EIFLDSPERK KSNFNCFYNG
260 270 280
SAQTVNIDFS ISGSRESCVS LFLVVLLGLL VQQLTLAEL
Length:289
Mass (Da):33,124
Last modified:June 13, 2012 - v2
Checksum:iBA3748FA37636051
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2T → P in CAA60948 (PubMed:8811076).Curated1
Sequence conflicti2T → P in AAB71683 (PubMed:9300695).Curated1
Sequence conflicti2T → P in AAB71684 (PubMed:9300695).Curated1
Sequence conflicti4K → N in AAB71683 (PubMed:9300695).Curated1
Sequence conflicti4K → N in AAB71684 (PubMed:9300695).Curated1
Sequence conflicti5I → N in CAA60948 (PubMed:8811076).Curated1
Sequence conflicti5I → N in AAB71683 (PubMed:9300695).Curated1
Sequence conflicti5I → N in AAB71684 (PubMed:9300695).Curated1
Sequence conflicti40S → G in CAA60948 (PubMed:8811076).Curated1
Sequence conflicti115K → I in CAA60948 (PubMed:8811076).Curated1
Sequence conflicti231E → Q in CAA60948 (PubMed:8811076).Curated1
Sequence conflicti289L → P in CAA60948 (PubMed:8811076).Curated1
Sequence conflicti289L → P in AAB71683 (PubMed:9300695).Curated1
Sequence conflicti289L → P in AAB71684 (PubMed:9300695).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87612 mRNA. Translation: CAA60948.1.
AF016463 mRNA. Translation: AAB71683.1.
AF016465 mRNA. Translation: AAB71684.1.
AC129609 Genomic DNA. No translation available.
CCDSiCCDS21512.1.
RefSeqiNP_064299.2. NM_019915.2.
XP_006507319.1. XM_006507256.2.
UniGeneiMm.57008.

Genome annotation databases

EnsembliENSMUST00000063920; ENSMUSP00000065658; ENSMUSG00000030651.
GeneIDi11872.
KEGGimmu:11872.
UCSCiuc009ioy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87612 mRNA. Translation: CAA60948.1.
AF016463 mRNA. Translation: AAB71683.1.
AF016465 mRNA. Translation: AAB71684.1.
AC129609 Genomic DNA. No translation available.
CCDSiCCDS21512.1.
RefSeqiNP_064299.2. NM_019915.2.
XP_006507319.1. XM_006507256.2.
UniGeneiMm.57008.

3D structure databases

ProteinModelPortaliO35975.
SMRiO35975.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000065658.

PTM databases

iPTMnetiO35975.
PhosphoSitePlusiO35975.
SwissPalmiO35975.

Proteomic databases

PaxDbiO35975.
PRIDEiO35975.

Protocols and materials databases

DNASUi11872.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063920; ENSMUSP00000065658; ENSMUSG00000030651.
GeneIDi11872.
KEGGimmu:11872.
UCSCiuc009ioy.1. mouse.

Organism-specific databases

CTDi11872.
MGIiMGI:107545. Art2b.

Phylogenomic databases

eggNOGiENOG410J95D. Eukaryota.
ENOG410ZKT4. LUCA.
GeneTreeiENSGT00530000062975.
HOGENOMiHOG000273888.
HOVERGENiHBG004464.
InParanoidiO35975.
KOiK19980.
OMAiICAYTAG.
OrthoDBiEOG091G0BSN.
TreeFamiTF335356.

Miscellaneous databases

PROiO35975.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030651.
CleanExiMM_ART2B.
GenevisibleiO35975. MM.

Family and domain databases

InterProiIPR000768. ART.
[Graphical view]
PfamiPF01129. ART. 1 hit.
[Graphical view]
PRINTSiPR00970. RIBTRNSFRASE.
PROSITEiPS01291. ART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAR2B_MOUSE
AccessioniPrimary (citable) accession number: O35975
Secondary accession number(s): F8VQK8, O35702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 13, 2012
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.