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O35969 (GAMT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanidinoacetate N-methyltransferase

EC=2.1.1.2
Gene names
Name:Gamt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.

Pathway

Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 2/2.

Subunit structure

Monomer By similarity.

Subcellular location

Cell projectionmicrovillus. Note: Detected in microvilli of the epithelial cells lining the caput epididymis. Ref.4

Tissue specificity

Highly expressed in testis, caput epididymis, ovary, and liver. In the testis, localized primarily in Sertoli cells. Expressed in brain with high levels in oligodendrocytes and olfactory ensheathing glia. Moderate levels of expression in astrocytes. Ref.4 Ref.5

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.

Contains 1 RMT2 (arginine N-methyltransferase 2-like) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35969-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35969-2)

The sequence of this isoform differs from the canonical sequence as follows:
     153-153: K → KLSSHGPTPSCPLASLQ
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 236235Guanidinoacetate N-methyltransferase
PRO_0000087431

Regions

Domain13 – 236224RMT2
Region69 – 746S-adenosyl-L-methionine binding By similarity
Region90 – 923S-adenosyl-L-methionine By similarity
Region117 – 1182S-adenosyl-L-methionine binding By similarity
Region171 – 1722Substrate binding By similarity

Sites

Binding site201S-adenosyl-L-methionine By similarity
Binding site421Substrate By similarity
Binding site461Substrate By similarity
Binding site501S-adenosyl-L-methionine By similarity
Binding site1351S-adenosyl-L-methionine and substrate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity

Natural variations

Alternative sequence1531K → KLSSHGPTPSCPLASLQ in isoform 2.
VSP_017727

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 3D982DE5D51DF5CD

FASTA23626,336
        10         20         30         40         50         60 
MSSSAASPLF APGEDCGPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM HALAAAAASR 

        70         80         90        100        110        120 
GGRVLEVGFG MAIAASRVQQ APIEEHWIIE CNDGVFQRLQ DWALRQPHKV VPLKGLWEEV 

       130        140        150        160        170        180 
APTLPDGHFD GILYDTYPLS EEAWHTHQFN FIKNHAFRLL KTGGVLTYCN LTSWGELMKS 

       190        200        210        220        230 
KYTDITTMFE ETQVPALQEA GFLKENICTE VMALVPPADC RYYAFPQMIT PLVTKH 

« Hide

Isoform 2 [UniParc].

Checksum: 2F7006B71CFA8FF1
Show »

FASTA25227,913

References

« Hide 'large scale' references
[1]"The human guanidinoacetate methyltransferase (GAMT) gene maps to a syntenic region on 19p13.3, homologous to band C of mouse chromosome 10, but GAMT is not mutated in jittery mice."
Jenne D.E., Olsen A.S., Zimmer M.
Biochem. Biophys. Res. Commun. 238:723-727(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Cerebellum and Inner ear.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Liver.
[4]"Guanidinoacetate methyltransferase in the mouse: extensive expression in Sertoli cells of testis and in microvilli of caput epididymis."
Lee H., Ogawa H., Fujioka M., Gerton G.L.
Biol. Reprod. 50:152-162(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]"Distinct cellular expressions of creatine synthetic enzyme GAMT and creatine kinases uCK-Mi and CK-B suggest a novel neuron-glial relationship for brain energy homeostasis."
Tachikawa M., Fukaya M., Terasaki T., Ohtsuki S., Watanabe M.
Eur. J. Neurosci. 20:144-160(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF015887 mRNA. Translation: AAB81495.1.
AF010499, AF010498 Genomic DNA. Translation: AAB81498.1.
AK140688 mRNA. Translation: BAE24443.1.
AK158087 mRNA. Translation: BAE34352.1.
BC049233 mRNA. Translation: AAH49233.1.
PIRJC5664.
RefSeqNP_034385.1. NM_010255.3.
XP_006513284.1. XM_006513221.1.
UniGeneMm.7329.

3D structure databases

ProteinModelPortalO35969.
SMRO35969. Positions 8-236.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35969. 2 interactions.
MINTMINT-1869591.

PTM databases

PhosphoSiteO35969.

2D gel databases

REPRODUCTION-2DPAGEIPI00742399.
O35969.
SWISS-2DPAGEO35969.

Proteomic databases

PaxDbO35969.
PRIDEO35969.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020359; ENSMUSP00000020359; ENSMUSG00000020150. [O35969-2]
ENSMUST00000105363; ENSMUSP00000101002; ENSMUSG00000020150. [O35969-1]
GeneID14431.
KEGGmmu:14431.
UCSCuc007gcj.2. mouse. [O35969-1]
uc011xio.1. mouse. [O35969-2]

Organism-specific databases

CTD2593.
MGIMGI:1098221. Gamt.

Phylogenomic databases

eggNOGNOG235457.
GeneTreeENSGT00390000018061.
HOGENOMHOG000010290.
HOVERGENHBG005801.
KOK00542.
OMAFEETQVP.
OrthoDBEOG75QR4S.
PhylomeDBO35969.
TreeFamTF328555.

Enzyme and pathway databases

UniPathwayUPA00104; UER00580.

Gene expression databases

BgeeO35969.
CleanExMM_GAMT.
GenevestigatorO35969.

Family and domain databases

InterProIPR016550. GuanidinoAc_N-MeTrfase.
IPR026480. RMT2_dom.
[Graphical view]
PIRSFPIRSF009285. GAMT. 1 hit.
PROSITEPS51559. SAM_RMT2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286049.
PROO35969.
SOURCESearch...

Entry information

Entry nameGAMT_MOUSE
AccessionPrimary (citable) accession number: O35969
Secondary accession number(s): Q3TZ58, Q3US90
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot