O35969 (GAMT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanidinoacetate N-methyltransferase
EC=2.1.1.2

Gene names

Name:

Gamt

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.
Pathway	Amine and polyamine biosynthesis; creatine biosynthesis; creatine from L-arginine and glycine: step 2/2.
Subunit structure	Monomer By similarity.
Subcellular location	Cell projection › microvillus. Note: Detected in microvilli of the epithelial cells lining the caput epididymis. Ref.4
Tissue specificity	Highly expressed in testis, caput epididymis, ovary, and liver. In the testis, localized primarily in Sertoli cells. Expressed in brain with high levels in oligodendrocytes and olfactory ensheathing glia. Moderate levels of expression in astrocytes. Ref.4 Ref.5
Sequence similarities	Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family. Contains 1 RMT2 (arginine N-methyltransferase 2-like) domain.

Ontologies

Keywords
Cellular component	Cell projection
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	creatine biosynthetic process Inferred from mutant phenotype PubMed 15028668. Source: MGI organ morphogenesis Inferred from mutant phenotype PubMed 15028668. Source: MGI regulation of multicellular organism growth Inferred from mutant phenotype PubMed 15028668. Source: MGI spermatogenesis Inferred from mutant phenotype PubMed 15028668. Source: MGI
Cellular_component	microvillus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	guanidinoacetate N-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O35969-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O35969-2)

The sequence of this isoform differs from the canonical sequence as follows:
     153-153: K → KLSSHGPTPSCPLASLQ

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Guanidinoacetate N-methyltransferase		PRO_0000087431
Regions
	Domain	13 – 236	224	RMT2
	Region	69 – 74	6	S-adenosyl-L-methionine binding By similarity
	Region	90 – 92	3	S-adenosyl-L-methionine By similarity
	Region	117 – 118	2	S-adenosyl-L-methionine binding By similarity
	Region	171 – 172	2	Substrate binding By similarity
Sites
	Binding site	20	1	S-adenosyl-L-methionine By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	46	1	Substrate By similarity
	Binding site	50	1	S-adenosyl-L-methionine By similarity
	Binding site	135	1	S-adenosyl-L-methionine and substrate By similarity
Natural variations
	Alternative sequence	153	1	K → KLSSHGPTPSCPLASLQ in isoform 2.		VSP_017727

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3D982DE5D51DF5CD	FASTA	236	26,336
	10 20 30 40 50 60 MSSSAASPLF APGEDCGPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM HALAAAAASR 70 80 90 100 110 120 GGRVLEVGFG MAIAASRVQQ APIEEHWIIE CNDGVFQRLQ DWALRQPHKV VPLKGLWEEV 130 140 150 160 170 180 APTLPDGHFD GILYDTYPLS EEAWHTHQFN FIKNHAFRLL KTGGVLTYCN LTSWGELMKS 190 200 210 220 230 KYTDITTMFE ETQVPALQEA GFLKENICTE VMALVPPADC RYYAFPQMIT PLVTKH
	Isoform 2 [UniParc]. Checksum: 2F7006B71CFA8FF1	FASTA	252	27,913
	10 20 30 40 50 60 MSSSAASPLF APGEDCGPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM HALAAAAASR 70 80 90 100 110 120 GGRVLEVGFG MAIAASRVQQ APIEEHWIIE CNDGVFQRLQ DWALRQPHKV VPLKGLWEEV 130 140 150 160 170 180 APTLPDGHFD GILYDTYPLS EEAWHTHQFN FIKLSSHGPT PSCPLASLQN HAFRLLKTGG 190 200 210 220 230 240 VLTYCNLTSW GELMKSKYTD ITTMFEETQV PALQEAGFLK ENICTEVMAL VPPADCRYYA 250 FPQMITPLVT KH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human guanidinoacetate methyltransferase (GAMT) gene maps to a syntenic region on 19p13.3, homologous to band C of mouse chromosome 10, but GAMT is not mutated in jittery mice." Jenne D.E., Olsen A.S., Zimmer M. Biochem. Biophys. Res. Commun. 238:723-727(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1). Strain: C57BL/6.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cerebellum and Inner ear.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Liver.
[4]	"Guanidinoacetate methyltransferase in the mouse: extensive expression in Sertoli cells of testis and in microvilli of caput epididymis." Lee H., Ogawa H., Fujioka M., Gerton G.L. Biol. Reprod. 50:152-162(1994) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]	"Distinct cellular expressions of creatine synthetic enzyme GAMT and creatine kinases uCK-Mi and CK-B suggest a novel neuron-glial relationship for brain energy homeostasis." Tachikawa M., Fukaya M., Terasaki T., Ohtsuki S., Watanabe M. Eur. J. Neurosci. 20:144-160(2004) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF015887 mRNA. Translation: AAB81495.1. AF010499, AF010498 Genomic DNA. Translation: AAB81498.1. AK140688 mRNA. Translation: BAE24443.1. AK158087 mRNA. Translation: BAE34352.1. BC049233 mRNA. Translation: AAH49233.1.
IPI	IPI00136274. IPI00742399.
PIR	JC5664.
RefSeq	NP_034385.1. NM_010255.3.
UniGene	Mm.7329.
3D structure databases
ProteinModelPortal	O35969.
SMR	O35969. Positions 8-236.
ModBase	Search...
PTM databases
PhosphoSite	O35969.
2D gel databases
REPRODUCTION-2DPAGE	IPI00742399. O35969.
SWISS-2DPAGE	O35969.
Proteomic databases
PaxDb	O35969.
PRIDE	O35969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020359; ENSMUSP00000020359; ENSMUSG00000020150. ENSMUST00000105363; ENSMUSP00000101002; ENSMUSG00000020150.
GeneID	14431.
KEGG	mmu:14431.
UCSC	uc007gcj.2. mouse. uc011xio.1. mouse.
Organism-specific databases
CTD	2593.
MGI	MGI:1098221. Gamt.
Phylogenomic databases
eggNOG	NOG235457.
GeneTree	ENSGT00390000018061.
HOGENOM	HOG000010290.
HOVERGEN	HBG005801.
KO	K00542.
OMA	FEETQVP.
OrthoDB	EOG4XSKQP.
Enzyme and pathway databases
UniPathway	UPA00104; UER00580.
Gene expression databases
Bgee	O35969.
CleanEx	MM_GAMT.
Genevestigator	O35969.
GermOnline	ENSMUSG00000020150. Mus musculus.
Family and domain databases
InterPro	IPR016550. GuanidinoAc_N-MeTrfase. IPR026480. RMT2_dom. [Graphical view]
PIRSF	PIRSF009285. GAMT. 1 hit.
PROSITE	PS51559. SAM_RMT2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	286049.
SOURCE	Search...

Entry information

Entry name

GAMT_MOUSE

Accession

Primary (citable) accession number: O35969
Secondary accession number(s): Q3TZ58, Q3US90

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program