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O35969

- GAMT_MOUSE

UniProt

O35969 - GAMT_MOUSE

Protein

Guanidinoacetate N-methyltransferase

Gene

Gamt

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei42 – 421SubstratePROSITE-ProRule annotation
    Binding sitei46 – 461SubstratePROSITE-ProRule annotation
    Binding sitei50 – 501S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei135 – 1351S-adenosyl-L-methionine and substratePROSITE-ProRule annotation

    GO - Molecular functioni

    1. guanidinoacetate N-methyltransferase activity Source: UniProtKB

    GO - Biological processi

    1. creatine biosynthetic process Source: MGI
    2. organ morphogenesis Source: MGI
    3. regulation of multicellular organism growth Source: MGI
    4. spermatogenesis Source: MGI

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00104; UER00580.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanidinoacetate N-methyltransferase (EC:2.1.1.2)
    Gene namesi
    Name:Gamt
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1098221. Gamt.

    Subcellular locationi

    Cell projectionmicrovillus 1 Publication
    Note: Detected in microvilli of the epithelial cells lining the caput epididymis.

    GO - Cellular componenti

    1. microvillus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 236235Guanidinoacetate N-methyltransferasePRO_0000087431Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO35969.
    PaxDbiO35969.
    PRIDEiO35969.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00742399.
    O35969.
    SWISS-2DPAGEO35969.

    PTM databases

    PhosphoSiteiO35969.

    Expressioni

    Tissue specificityi

    Highly expressed in testis, caput epididymis, ovary, and liver. In the testis, localized primarily in Sertoli cells. Expressed in brain with high levels in oligodendrocytes and olfactory ensheathing glia. Moderate levels of expression in astrocytes.2 Publications

    Gene expression databases

    BgeeiO35969.
    CleanExiMM_GAMT.
    GenevestigatoriO35969.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    IntActiO35969. 2 interactions.
    MINTiMINT-1869591.

    Structurei

    3D structure databases

    ProteinModelPortaliO35969.
    SMRiO35969. Positions 8-236.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 236224RMT2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 746S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni90 – 923S-adenosyl-L-methioninePROSITE-ProRule annotation
    Regioni117 – 1182S-adenosyl-L-methionine bindingPROSITE-ProRule annotation
    Regioni171 – 1722Substrate bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.PROSITE-ProRule annotation
    Contains 1 RMT2 (arginine N-methyltransferase 2-like) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG235457.
    GeneTreeiENSGT00390000018061.
    HOGENOMiHOG000010290.
    HOVERGENiHBG005801.
    KOiK00542.
    OMAiRYYAFPQ.
    OrthoDBiEOG75QR4S.
    PhylomeDBiO35969.
    TreeFamiTF328555.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR016550. GuanidinoAc_N-MeTrfase.
    IPR026480. RMT2_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PIRSFiPIRSF009285. GAMT. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51559. SAM_RMT2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O35969-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSSAASPLF APGEDCGPAW RAAPAAYDAS DTHLQILGKP VMERWETPYM    50
    HALAAAAASR GGRVLEVGFG MAIAASRVQQ APIEEHWIIE CNDGVFQRLQ 100
    DWALRQPHKV VPLKGLWEEV APTLPDGHFD GILYDTYPLS EEAWHTHQFN 150
    FIKNHAFRLL KTGGVLTYCN LTSWGELMKS KYTDITTMFE ETQVPALQEA 200
    GFLKENICTE VMALVPPADC RYYAFPQMIT PLVTKH 236
    Length:236
    Mass (Da):26,336
    Last modified:January 1, 1998 - v1
    Checksum:i3D982DE5D51DF5CD
    GO
    Isoform 2 (identifier: O35969-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         153-153: K → KLSSHGPTPSCPLASLQ

    Note: No experimental confirmation available.

    Show »
    Length:252
    Mass (Da):27,913
    Checksum:i2F7006B71CFA8FF1
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei153 – 1531K → KLSSHGPTPSCPLASLQ in isoform 2. 1 PublicationVSP_017727

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF015887 mRNA. Translation: AAB81495.1.
    AF010499, AF010498 Genomic DNA. Translation: AAB81498.1.
    AK140688 mRNA. Translation: BAE24443.1.
    AK158087 mRNA. Translation: BAE34352.1.
    BC049233 mRNA. Translation: AAH49233.1.
    CCDSiCCDS35976.1. [O35969-1]
    PIRiJC5664.
    RefSeqiNP_034385.1. NM_010255.3. [O35969-1]
    XP_006513284.1. XM_006513221.1. [O35969-2]
    UniGeneiMm.7329.

    Genome annotation databases

    EnsembliENSMUST00000020359; ENSMUSP00000020359; ENSMUSG00000020150. [O35969-2]
    ENSMUST00000105363; ENSMUSP00000101002; ENSMUSG00000020150. [O35969-1]
    GeneIDi14431.
    KEGGimmu:14431.
    UCSCiuc007gcj.2. mouse. [O35969-1]
    uc011xio.1. mouse. [O35969-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF015887 mRNA. Translation: AAB81495.1 .
    AF010499 , AF010498 Genomic DNA. Translation: AAB81498.1 .
    AK140688 mRNA. Translation: BAE24443.1 .
    AK158087 mRNA. Translation: BAE34352.1 .
    BC049233 mRNA. Translation: AAH49233.1 .
    CCDSi CCDS35976.1. [O35969-1 ]
    PIRi JC5664.
    RefSeqi NP_034385.1. NM_010255.3. [O35969-1 ]
    XP_006513284.1. XM_006513221.1. [O35969-2 ]
    UniGenei Mm.7329.

    3D structure databases

    ProteinModelPortali O35969.
    SMRi O35969. Positions 8-236.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35969. 2 interactions.
    MINTi MINT-1869591.

    PTM databases

    PhosphoSitei O35969.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00742399.
    O35969.
    SWISS-2DPAGE O35969.

    Proteomic databases

    MaxQBi O35969.
    PaxDbi O35969.
    PRIDEi O35969.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020359 ; ENSMUSP00000020359 ; ENSMUSG00000020150 . [O35969-2 ]
    ENSMUST00000105363 ; ENSMUSP00000101002 ; ENSMUSG00000020150 . [O35969-1 ]
    GeneIDi 14431.
    KEGGi mmu:14431.
    UCSCi uc007gcj.2. mouse. [O35969-1 ]
    uc011xio.1. mouse. [O35969-2 ]

    Organism-specific databases

    CTDi 2593.
    MGIi MGI:1098221. Gamt.

    Phylogenomic databases

    eggNOGi NOG235457.
    GeneTreei ENSGT00390000018061.
    HOGENOMi HOG000010290.
    HOVERGENi HBG005801.
    KOi K00542.
    OMAi RYYAFPQ.
    OrthoDBi EOG75QR4S.
    PhylomeDBi O35969.
    TreeFami TF328555.

    Enzyme and pathway databases

    UniPathwayi UPA00104 ; UER00580 .

    Miscellaneous databases

    NextBioi 286049.
    PROi O35969.
    SOURCEi Search...

    Gene expression databases

    Bgeei O35969.
    CleanExi MM_GAMT.
    Genevestigatori O35969.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR016550. GuanidinoAc_N-MeTrfase.
    IPR026480. RMT2_dom.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PIRSFi PIRSF009285. GAMT. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51559. SAM_RMT2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human guanidinoacetate methyltransferase (GAMT) gene maps to a syntenic region on 19p13.3, homologous to band C of mouse chromosome 10, but GAMT is not mutated in jittery mice."
      Jenne D.E., Olsen A.S., Zimmer M.
      Biochem. Biophys. Res. Commun. 238:723-727(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Strain: C57BL/6.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Cerebellum and Inner ear.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Liver.
    4. "Guanidinoacetate methyltransferase in the mouse: extensive expression in Sertoli cells of testis and in microvilli of caput epididymis."
      Lee H., Ogawa H., Fujioka M., Gerton G.L.
      Biol. Reprod. 50:152-162(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    5. "Distinct cellular expressions of creatine synthetic enzyme GAMT and creatine kinases uCK-Mi and CK-B suggest a novel neuron-glial relationship for brain energy homeostasis."
      Tachikawa M., Fukaya M., Terasaki T., Ohtsuki S., Watanabe M.
      Eur. J. Neurosci. 20:144-160(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGAMT_MOUSE
    AccessioniPrimary (citable) accession number: O35969
    Secondary accession number(s): Q3TZ58, Q3US90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3