Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rab-33B

Gene

Rab33b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking (By similarity). It is involved in autophagy, acting as a modulator of autophagosome formation.By similarity1 Publication

Enzyme regulationi

Regulated by a guanine nucleotide-exchange factor (GEF) and a GTPase-activating protein (GAP) and alternates between an inactive GDP-bound and an active GTP-bound form. In vitro, SGSM2 acts as its GAP and inactivates it by stimulating its GTPase activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46GTP8
Nucleotide bindingi88 – 92GTP5
Nucleotide bindingi148 – 151GTP4
Nucleotide bindingi178 – 180GTP3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-33B
Gene namesi
Name:Rab33b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1330805. Rab33b.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi lumen Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47T → N: Affects interaction with ATG16L. 1 Publication1
Mutagenesisi92Q → L: Does not affect interaction with ATG16L. Induces lipidation of LC3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001212401 – 229Ras-related protein Rab-33BAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi227S-geranylgeranyl cysteineBy similarity1
Modified residuei229Cysteine methyl esterBy similarity1
Lipidationi229S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiO35963.
PeptideAtlasiO35963.
PRIDEiO35963.

PTM databases

iPTMnetiO35963.
PhosphoSitePlusiO35963.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000027739.
CleanExiMM_RAB33B.
ExpressionAtlasiO35963. baseline and differential.
GenevisibleiO35963. MM.

Interactioni

Subunit structurei

Interacts with RIC1 (via C-terminus domain); the interaction is direct with a preference for RAB33B-GTP. Interacts with RGP1 (By similarity). Interacts with ATG16L; the interaction is important for autophagosome formation (PubMed:18448665).By similarity1 Publication

Protein-protein interaction databases

BioGridi202543. 6 interactors.
DIPiDIP-60512N.
IntActiO35963. 21 interactors.
MINTiMINT-4996206.
STRINGi10090.ENSMUSP00000063054.

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 39Combined sources7
Helixi46 – 55Combined sources10
Beta strandi70 – 77Combined sources8
Beta strandi80 – 88Combined sources9
Helixi93 – 96Combined sources4
Turni97 – 99Combined sources3
Helixi100 – 104Combined sources5
Beta strandi109 – 115Combined sources7
Helixi119 – 123Combined sources5
Helixi125 – 135Combined sources11
Beta strandi139 – 141Combined sources3
Beta strandi143 – 148Combined sources6
Helixi153 – 155Combined sources3
Helixi160 – 169Combined sources10
Beta strandi174 – 176Combined sources3
Beta strandi179 – 181Combined sources3
Helixi182 – 185Combined sources4
Helixi188 – 195Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z06X-ray1.81A30-202[»]
2G77X-ray2.26B14-202[»]
ProteinModelPortaliO35963.
SMRiO35963.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35963.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
GeneTreeiENSGT00860000133754.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiO35963.
KOiK07920.
OMAiPAWIEEC.
OrthoDBiEOG091G0FVM.
TreeFamiTF300097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEMESSLE VSFSSSCAVS GASGCLPPAR SRIFKIIVIG DSNVGKTCLT
60 70 80 90 100
YRFCAGRFPD RTEATIGVDF RERAVDIDGE RIKIQLWDTA GQERFRKSMV
110 120 130 140 150
QHYYRNVHAV VFVYDMTNMA SFHSLPAWIE ECKQHLLAND IPRILVGNKC
160 170 180 190 200
DLRSAIQVPT DLAQKFADTH SMPLFETSAK NPNDNDHVEA IFMTLAHKLK
210 220
SHKPLMLSQL PDNRISLKPE TKPAVTCWC
Length:229
Mass (Da):25,767
Last modified:January 1, 1998 - v1
Checksum:iEE2B3E482FC4365A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004665 mRNA. Translation: BAA22655.1.
AB004664 Genomic DNA. Translation: BAA22654.1.
AK004974 mRNA. Translation: BAB23710.1.
AK087967 mRNA. Translation: BAC40064.1.
AK139821 mRNA. Translation: BAE24147.1.
AK159674 mRNA. Translation: BAE35278.1.
BC065076 mRNA. Translation: AAH65076.1.
CCDSiCCDS38427.1.
RefSeqiNP_058554.1. NM_016858.2.
UniGeneiMm.1664.

Genome annotation databases

EnsembliENSMUST00000054387; ENSMUSP00000063054; ENSMUSG00000027739.
GeneIDi19338.
KEGGimmu:19338.
UCSCiuc008pea.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004665 mRNA. Translation: BAA22655.1.
AB004664 Genomic DNA. Translation: BAA22654.1.
AK004974 mRNA. Translation: BAB23710.1.
AK087967 mRNA. Translation: BAC40064.1.
AK139821 mRNA. Translation: BAE24147.1.
AK159674 mRNA. Translation: BAE35278.1.
BC065076 mRNA. Translation: AAH65076.1.
CCDSiCCDS38427.1.
RefSeqiNP_058554.1. NM_016858.2.
UniGeneiMm.1664.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z06X-ray1.81A30-202[»]
2G77X-ray2.26B14-202[»]
ProteinModelPortaliO35963.
SMRiO35963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202543. 6 interactors.
DIPiDIP-60512N.
IntActiO35963. 21 interactors.
MINTiMINT-4996206.
STRINGi10090.ENSMUSP00000063054.

PTM databases

iPTMnetiO35963.
PhosphoSitePlusiO35963.

Proteomic databases

PaxDbiO35963.
PeptideAtlasiO35963.
PRIDEiO35963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054387; ENSMUSP00000063054; ENSMUSG00000027739.
GeneIDi19338.
KEGGimmu:19338.
UCSCiuc008pea.1. mouse.

Organism-specific databases

CTDi83452.
MGIiMGI:1330805. Rab33b.

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
GeneTreeiENSGT00860000133754.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiO35963.
KOiK07920.
OMAiPAWIEEC.
OrthoDBiEOG091G0FVM.
TreeFamiTF300097.

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiO35963.
PROiO35963.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027739.
CleanExiMM_RAB33B.
ExpressionAtlasiO35963. baseline and differential.
GenevisibleiO35963. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRB33B_MOUSE
AccessioniPrimary (citable) accession number: O35963
Secondary accession number(s): Q3TWI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.