Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ras-related protein Rab-33B

Gene

Rab33b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein transport. Acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking (By similarity). It is involved in autophagy, acting as a modulator of autophagosome formation.By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 468GTP
Nucleotide bindingi88 – 925GTP
Nucleotide bindingi148 – 1514GTP
Nucleotide bindingi178 – 1803GTP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-33B
Gene namesi
Name:Rab33b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1330805. Rab33b.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • Golgi apparatus Source: MGI
  • Golgi lumen Source: UniProtKB
  • Golgi membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi47 – 471T → N: Affects interaction with ATG16L. 1 Publication
Mutagenesisi92 – 921Q → L: Does not affect interaction with ATG16L. Induces lipidation of LC3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229Ras-related protein Rab-33BPRO_0000121240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi227 – 2271S-geranylgeranyl cysteineBy similarity
Modified residuei229 – 2291Cysteine methyl esterBy similarity
Lipidationi229 – 2291S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiO35963.
PeptideAtlasiO35963.
PRIDEiO35963.

PTM databases

iPTMnetiO35963.
PhosphoSiteiO35963.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiO35963.
CleanExiMM_RAB33B.
ExpressionAtlasiO35963. baseline and differential.
GenevisibleiO35963. MM.

Interactioni

Subunit structurei

Interacts with RIC1 (via C-terminus domain); the interaction is direct with a preference for RAB33B-GTP. Interacts with RGP1 (By similarity). Interacts with ATG16L; the interaction is important for autophagosome formation.By similarity2 Publications

Protein-protein interaction databases

BioGridi202543. 6 interactions.
DIPiDIP-60512N.
IntActiO35963. 21 interactions.
MINTiMINT-4996206.
STRINGi10090.ENSMUSP00000063054.

Structurei

Secondary structure

1
229
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 397Combined sources
Helixi46 – 5510Combined sources
Beta strandi70 – 778Combined sources
Beta strandi80 – 889Combined sources
Helixi93 – 964Combined sources
Turni97 – 993Combined sources
Helixi100 – 1045Combined sources
Beta strandi109 – 1157Combined sources
Helixi119 – 1235Combined sources
Helixi125 – 13511Combined sources
Beta strandi139 – 1413Combined sources
Beta strandi143 – 1486Combined sources
Helixi153 – 1553Combined sources
Helixi160 – 16910Combined sources
Beta strandi174 – 1763Combined sources
Beta strandi179 – 1813Combined sources
Helixi182 – 1854Combined sources
Helixi188 – 1958Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z06X-ray1.81A30-202[»]
2G77X-ray2.26B14-202[»]
ProteinModelPortaliO35963.
SMRiO35963. Positions 30-202.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35963.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiO35963.
KOiK07920.
OMAiPAWIEEC.
OrthoDBiEOG78PVB5.
TreeFamiTF300097.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEMESSLE VSFSSSCAVS GASGCLPPAR SRIFKIIVIG DSNVGKTCLT
60 70 80 90 100
YRFCAGRFPD RTEATIGVDF RERAVDIDGE RIKIQLWDTA GQERFRKSMV
110 120 130 140 150
QHYYRNVHAV VFVYDMTNMA SFHSLPAWIE ECKQHLLAND IPRILVGNKC
160 170 180 190 200
DLRSAIQVPT DLAQKFADTH SMPLFETSAK NPNDNDHVEA IFMTLAHKLK
210 220
SHKPLMLSQL PDNRISLKPE TKPAVTCWC
Length:229
Mass (Da):25,767
Last modified:January 1, 1998 - v1
Checksum:iEE2B3E482FC4365A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004665 mRNA. Translation: BAA22655.1.
AB004664 Genomic DNA. Translation: BAA22654.1.
AK004974 mRNA. Translation: BAB23710.1.
AK087967 mRNA. Translation: BAC40064.1.
AK139821 mRNA. Translation: BAE24147.1.
AK159674 mRNA. Translation: BAE35278.1.
BC065076 mRNA. Translation: AAH65076.1.
CCDSiCCDS38427.1.
RefSeqiNP_058554.1. NM_016858.2.
UniGeneiMm.1664.

Genome annotation databases

EnsembliENSMUST00000054387; ENSMUSP00000063054; ENSMUSG00000027739.
GeneIDi19338.
KEGGimmu:19338.
UCSCiuc008pea.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004665 mRNA. Translation: BAA22655.1.
AB004664 Genomic DNA. Translation: BAA22654.1.
AK004974 mRNA. Translation: BAB23710.1.
AK087967 mRNA. Translation: BAC40064.1.
AK139821 mRNA. Translation: BAE24147.1.
AK159674 mRNA. Translation: BAE35278.1.
BC065076 mRNA. Translation: AAH65076.1.
CCDSiCCDS38427.1.
RefSeqiNP_058554.1. NM_016858.2.
UniGeneiMm.1664.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z06X-ray1.81A30-202[»]
2G77X-ray2.26B14-202[»]
ProteinModelPortaliO35963.
SMRiO35963. Positions 30-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202543. 6 interactions.
DIPiDIP-60512N.
IntActiO35963. 21 interactions.
MINTiMINT-4996206.
STRINGi10090.ENSMUSP00000063054.

PTM databases

iPTMnetiO35963.
PhosphoSiteiO35963.

Proteomic databases

PaxDbiO35963.
PeptideAtlasiO35963.
PRIDEiO35963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054387; ENSMUSP00000063054; ENSMUSG00000027739.
GeneIDi19338.
KEGGimmu:19338.
UCSCiuc008pea.1. mouse.

Organism-specific databases

CTDi83452.
MGIiMGI:1330805. Rab33b.

Phylogenomic databases

eggNOGiKOG0084. Eukaryota.
ENOG410XQN5. LUCA.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiO35963.
KOiK07920.
OMAiPAWIEEC.
OrthoDBiEOG78PVB5.
TreeFamiTF300097.

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.

Miscellaneous databases

EvolutionaryTraceiO35963.
PROiO35963.
SOURCEiSearch...

Gene expression databases

BgeeiO35963.
CleanExiMM_RAB33B.
ExpressionAtlasiO35963. baseline and differential.
GenevisibleiO35963. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel Rab GTPase, Rab33B, is ubiquitously expressed and localized to the medial Golgi cisternae."
    Zheng J.Y., Koda T., Fujiwara T., Kishi M., Ikehara Y., Kakinuma M.
    J. Cell Sci. 111:1061-1069(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Egg, Liver and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 84-94, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  5. "Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation."
    Itoh T., Fujita N., Kanno E., Yamamoto A., Yoshimori T., Fukuda M.
    Mol. Biol. Cell 19:2916-2925(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG16L, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-47 AND GLN-92.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Lung.
  7. "Structural basis of family-wide Rab GTPase recognition by rabenosyn-5."
    Eathiraj S., Pan X., Ritacco C., Lambright D.G.
    Nature 436:415-419(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.81 ANGSTROMS) OF 30-202 IN COMPLEX WITH GTP ANALOG.

Entry informationi

Entry nameiRB33B_MOUSE
AccessioniPrimary (citable) accession number: O35963
Secondary accession number(s): Q3TWI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.