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Protein

Ras-related protein Rab-33B

Gene

Rab33b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protein transport. Acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking (By similarity). It is involved in autophagy, acting as a modulator of autophagosome formation.By similarity1 Publication

Enzyme regulationi

Regulated by a guanine nucleotide-exchange factor (GEF) and a GTPase-activating protein (GAP) and alternates between an inactive GDP-bound and an active GTP-bound form. In vitro, SGSM2 acts as its GAP and inactivates it by stimulating its GTPase activity.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46GTP8
Nucleotide bindingi88 – 92GTP5
Nucleotide bindingi148 – 151GTP4
Nucleotide bindingi178 – 180GTP3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processAutophagy, Protein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811438 Intra-Golgi traffic
R-MMU-8854214 TBC/RABGAPs
R-MMU-8873719 RAB geranylgeranylation

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-33B
Gene namesi
Name:Rab33b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1330805 Rab33b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47T → N: Affects interaction with ATG16L. 1 Publication1
Mutagenesisi92Q → L: Does not affect interaction with ATG16L. Induces lipidation of LC3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001212401 – 229Ras-related protein Rab-33BAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi227S-geranylgeranyl cysteineBy similarity1
Modified residuei229Cysteine methyl esterBy similarity1
Lipidationi229S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PaxDbiO35963
PeptideAtlasiO35963
PRIDEiO35963

PTM databases

iPTMnetiO35963
PhosphoSitePlusiO35963

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000027739
CleanExiMM_RAB33B
ExpressionAtlasiO35963 baseline and differential
GenevisibleiO35963 MM

Interactioni

Subunit structurei

Interacts with RIC1 (via C-terminus domain); the interaction is direct with a preference for RAB33B-GTP. Interacts with RGP1 (By similarity). Interacts with ATG16L; the interaction is important for autophagosome formation (PubMed:18448665).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GYP1Q084843EBI-6379521,EBI-8005From Saccharomyces cerevisiae (strain ATCC 204508 / S288c).

Protein-protein interaction databases

BioGridi202543, 7 interactors
DIPiDIP-60512N
IntActiO35963, 22 interactors
STRINGi10090.ENSMUSP00000063054

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 39Combined sources7
Helixi46 – 55Combined sources10
Beta strandi70 – 77Combined sources8
Beta strandi80 – 88Combined sources9
Helixi93 – 96Combined sources4
Turni97 – 99Combined sources3
Helixi100 – 104Combined sources5
Beta strandi109 – 115Combined sources7
Helixi119 – 123Combined sources5
Helixi125 – 135Combined sources11
Beta strandi139 – 141Combined sources3
Beta strandi143 – 148Combined sources6
Helixi153 – 155Combined sources3
Helixi160 – 169Combined sources10
Beta strandi174 – 176Combined sources3
Beta strandi179 – 181Combined sources3
Helixi182 – 185Combined sources4
Helixi188 – 195Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z06X-ray1.81A30-202[»]
2G77X-ray2.26B14-202[»]
ProteinModelPortaliO35963
SMRiO35963
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35963

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0084 Eukaryota
ENOG410XQN5 LUCA
GeneTreeiENSGT00900000140786
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiO35963
KOiK07920
OMAiPAWIEEC
OrthoDBiEOG091G0FVM
TreeFamiTF300097

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

O35963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEMESSLE VSFSSSCAVS GASGCLPPAR SRIFKIIVIG DSNVGKTCLT
60 70 80 90 100
YRFCAGRFPD RTEATIGVDF RERAVDIDGE RIKIQLWDTA GQERFRKSMV
110 120 130 140 150
QHYYRNVHAV VFVYDMTNMA SFHSLPAWIE ECKQHLLAND IPRILVGNKC
160 170 180 190 200
DLRSAIQVPT DLAQKFADTH SMPLFETSAK NPNDNDHVEA IFMTLAHKLK
210 220
SHKPLMLSQL PDNRISLKPE TKPAVTCWC
Length:229
Mass (Da):25,767
Last modified:January 1, 1998 - v1
Checksum:iEE2B3E482FC4365A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004665 mRNA Translation: BAA22655.1
AB004664 Genomic DNA Translation: BAA22654.1
AK004974 mRNA Translation: BAB23710.1
AK087967 mRNA Translation: BAC40064.1
AK139821 mRNA Translation: BAE24147.1
AK159674 mRNA Translation: BAE35278.1
BC065076 mRNA Translation: AAH65076.1
CCDSiCCDS38427.1
RefSeqiNP_058554.1, NM_016858.2
UniGeneiMm.1664

Genome annotation databases

EnsembliENSMUST00000054387; ENSMUSP00000063054; ENSMUSG00000027739
GeneIDi19338
KEGGimmu:19338
UCSCiuc008pea.1 mouse

Similar proteinsi

Entry informationi

Entry nameiRB33B_MOUSE
AccessioniPrimary (citable) accession number: O35963
Secondary accession number(s): Q3TWI8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health