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Protein

Hydroxyacylglutathione hydrolase, mitochondrial

Gene

Hagh

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (Glo1)
  2. Hydroxyacylglutathione hydrolase, mitochondrial (Hagh)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Zinc 1; via tele nitrogenBy similarity
Metal bindingi105 – 1051Zinc 1; via pros nitrogenBy similarity
Metal bindingi107 – 1071Zinc 2By similarity
Metal bindingi108 – 1081Zinc 2; via tele nitrogenBy similarity
Metal bindingi159 – 1591Zinc 1; via tele nitrogenBy similarity
Metal bindingi183 – 1831Zinc 1By similarity
Metal bindingi183 – 1831Zinc 2By similarity
Metal bindingi222 – 2221Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  • hydroxyacylglutathione hydrolase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • carbohydrate metabolic process Source: RGD
  • glutathione metabolic process Source: RGD
  • methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: InterPro
  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.2.6. 5301.
SABIO-RKO35952.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Round spermatid protein RSP29
Gene namesi
Name:Hagh
Synonyms:Rsp29
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2779. Hagh.

Subcellular locationi

Isoform 1 :
  • Mitochondrion matrix By similarity
Isoform 2 :
  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2262.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424MitochondrionSequence analysisAdd
BLAST
Chaini25 – 309285Hydroxyacylglutathione hydrolase, mitochondrialPRO_0000192344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei90 – 901N6-acetyllysineBy similarity
Modified residuei117 – 1171N6-acetyllysineBy similarity
Modified residuei230 – 2301N6-acetyllysine; alternateBy similarity
Modified residuei230 – 2301N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO35952.
PRIDEiO35952.

PTM databases

iPTMnetiO35952.
PhosphoSiteiO35952.

Expressioni

Tissue specificityi

Strongly expressed in testis, skeletal muscle and heart. Weakly expressed in placenta, pancreas, spleen and peripheral blood leukocytes.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020192.

Chemistry

BindingDBiO35952.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni192 – 1943Substrate bindingBy similarity
Regioni222 – 2243Substrate bindingBy similarity
Regioni298 – 3014Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
HOGENOMiHOG000058041.
HOVERGENiHBG001152.
InParanoidiO35952.
KOiK01069.
PhylomeDBiO35952.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform 1 (identifier: O35952-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLGRGSLCL RSLSVLGAAC ARRGLGQALL GLSLCHTDFR KNLTVQQDMM
60 70 80 90 100
KIELLPALTD NYMYLIIDED TQEAAVVDPV QPQKVIETVK KHRVKLTTVL
110 120 130 140 150
TTHHHWDHAG GNEKLVKLEP GLKVYGGDDR IGALTHKVTH LSTLEVGSLS
160 170 180 190 200
VKCLSTPCHT SGHICYFVSK PGSSEPSAVF TGDTLFVAGC GKFYEGTADE
210 220 230 240 250
MYKALLEVLG RLPPDTKVIC GHEYTVNNLK FARHVEPGNT AVQEKLAWAK
260 270 280 290 300
EKNAIGEPTV PSTLAEEFTY NPFMRVKEKT VQQHAGETDP VTTMRAIRRE

KDQFKVPRD
Length:309
Mass (Da):34,109
Last modified:September 1, 2009 - v2
Checksum:i625AD975E86E9B72
GO
Isoform 2 (identifier: O35952-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: Produced by alternative splicing. Also produced by alternative initiation at Met-50 of isoform 1. Alternative initiation has been proven in human.
Show »
Length:260
Mass (Da):28,896
Checksum:i8384898771085C1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451E → Q in FM068341 (Ref. 2) Curated
Sequence conflicti145 – 1451E → Q in AAH97301 (PubMed:15489334).Curated
Sequence conflicti173 – 1731S → N in FM068341 (Ref. 2) Curated
Sequence conflicti219 – 2191I → Y in AAH97301 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4949Missing in isoform 2. 2 PublicationsVSP_037932Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97667 Genomic DNA. Translation: AAC39944.1.
FM068341 mRNA. No translation available.
BC097301 mRNA. Translation: AAH97301.1.
PIRiJC5826.
RefSeqiNP_203500.2. NM_033349.2.
UniGeneiRn.11048.

Genome annotation databases

GeneIDi24439.
KEGGirno:24439.
UCSCiRGD:2779. rat. [O35952-1]

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97667 Genomic DNA. Translation: AAC39944.1.
FM068341 mRNA. No translation available.
BC097301 mRNA. Translation: AAH97301.1.
PIRiJC5826.
RefSeqiNP_203500.2. NM_033349.2.
UniGeneiRn.11048.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020192.

Chemistry

BindingDBiO35952.
ChEMBLiCHEMBL2262.

PTM databases

iPTMnetiO35952.
PhosphoSiteiO35952.

Proteomic databases

PaxDbiO35952.
PRIDEiO35952.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24439.
KEGGirno:24439.
UCSCiRGD:2779. rat. [O35952-1]

Organism-specific databases

CTDi3029.
RGDi2779. Hagh.

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
HOGENOMiHOG000058041.
HOVERGENiHBG001152.
InParanoidiO35952.
KOiK01069.
PhylomeDBiO35952.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
BRENDAi3.1.2.6. 5301.
SABIO-RKO35952.

Miscellaneous databases

PROiO35952.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2_RAT
AccessioniPrimary (citable) accession number: O35952
Secondary accession number(s): Q4V8M8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: September 1, 2009
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Only one single gene encoding glyoxalase II has been identified in vertebrates. In yeast and higher plants, separate genes encode the cytosolic and mitochondrial forms of glyoxalase II.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.