Serine/threonine-protein kinase Nek2

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Reviewed, UniProtKB/Swiss-Prot O35942 (NEK2_MOUSE)

Last modified June 16, 2009. Version 80. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase Nek2
    EC=2.7.11.1
Alternative name(s):
    NimA-related protein kinase 2

Gene names

Name:

Nek2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Protein kinase that is involved in mitotic regulation. May have a role at the G2-M transition. May also play a role in meiosis. Appears to play a role in centrosome splitting. Phosphorylates and activates NEK11 in G1/S-arrested cells.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Subunit structure	Interacts with TERF1. Homodimerizes By similarity.
Subcellular location	Nucleus. Centromere. Note: Localizes at the centromere. Ref.1
Tissue specificity	Most abundantly expressed in testis. Low levels found in mid-gestation embryo, ovary, placenta, intestine, thymus and skin. Within the testis, expression restricted to germ cells with highest levels detected in spermatocytes at pachytene and diplotene stages. Also expressed in meiotic pachytene oocytes. Ref.1 Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain.

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Ontologies

Keywords
Biological process	Cell cycle Cell division Meiosis Mitosis
Cellular component	Centromere Nucleus
Domain	Coiled coil
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW meiosis Inferred from electronic annotation. Source: UniProtKB-KW mitotic sister chromatid segregation Inferred from mutant phenotype. Source: MGI protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome, centromeric region Inferred from electronic annotation. Source: UniProtKB-SubCell condensed nuclear chromosome Inferred from direct assay. Source: MGI midbody Inferred from direct assay. Source: MGI spindle pole Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ppp1ca	P62137	1	EBI-1202268,EBI-357187

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 443

443

Serine/threonine-protein kinase Nek2

PRO_0000086422

Regions

Domain

8 – 271

264

Protein kinase

Nucleotide binding

14 – 22

ATP By similarity

Coiled coil

303 – 361

Potential

Coiled coil

403 – 427

Potential

Sites

Active site

141

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

170

Phosphothreonine; by autocatalysis By similarity

Modified residue

171

Phosphoserine; by autocatalysis By similarity

Modified residue

175

Phosphothreonine; by autocatalysis By similarity

Modified residue

179

Phosphothreonine; by autocatalysis By similarity

Modified residue

184

Phosphoserine By similarity

Modified residue

241

Phosphoserine; by autocatalysis By similarity

Modified residue

300

Phosphoserine By similarity

Modified residue

356

Phosphoserine By similarity

Modified residue

389

Phosphoserine By similarity

Modified residue

396

Phosphoserine By similarity

Modified residue

401

Phosphoserine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

O35942-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: DE09565C378307E1
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FASTA

443

51,308

        10         20         30         40         50         60 
MPSRVEDYEV LHSIGTGSYG RCQKIRRKSD GKILVWKELD YGSMTEVEKQ MLVSEVNLLR 

        70         80         90        100        110        120 
ELKHPNIVRY YDRIIDRTNT TLYIVMEYCE GGDLASVISK GTKDRQYLEE EFVLRVMTQL 

       130        140        150        160        170        180 
TLALKECHRR SDGGHTVLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTP 

       190        200        210        220        230        240 
YYMSPEQMSC LSYNEKSDIW SLACLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY 

       250        260        270        280        290        300 
SDGLNDLITR MLFLKDYHRP SVEEILESPL IADMVAEEQR RNLERRGRRS GEPSKLPDSS 

       310        320        330        340        350        360 
PVLSELKLKE SQLQDREQAL RAREDILEQK ERELCIRERL AEDKLARAES LMKNYSLLKE 

       370        380        390        400        410        420 
HRLLCLAGGP ELDLPSSAMK KKVHFHGESK ENTARSENSE SYLAKSKCRD LKKRLHAAQL 

       430        440 
RAQALADIEK NYQLKSRQIL GMR

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References

[1]	"The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes." Rhee K., Wolgemuth D.J. Development 124:2167-2177(1997) [PubMed: 9187143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: Swiss Webster. Tissue: Testis.
[2]	"Murine NIMA-related kinases are expressed in patterns suggesting distinct functions in gametogenesis and a role in the nervous system." Arama E., Yanai A., Kilfin G., Motro B. Oncogene 16:1813-1823(1998) [PubMed: 9583679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis." Tanaka K., Parvinen M., Nigg E.A. Exp. Cell Res. 237:264-274(1997) [PubMed: 9434622] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Brain.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U95610 mRNA. Translation: AAB67973.1. AF013166 mRNA. Translation: AAC35393.1. AF007247 mRNA. Translation: AAB70470.1.
IPI	IPI00136199.
UniGene	Mm.33773 Mm.403999
3D structure databases
HSSP	HSSP built from PDB template 1BLX based on UniProtKB Q00534.
SMR	O35942. Positions 3-273.
ModBase	Search...
Protein-protein interaction databases
IntAct	O35942. 1 interaction.
PTM databases
PhosphoSite	O35942.
Genome annotation databases
Ensembl	ENSMUSG00000026622. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:109359. Nek2.
Phylogenomic databases
HOGENOM	O35942.
HOVERGEN	O35942.
Enzyme and pathway databases
BRENDA	2.7.11.1. 244.
Gene expression databases
ArrayExpress	O35942.
Bgee	O35942.
GermOnline	ENSMUSG00000026622. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

NEK2_MOUSE

Accession

Primary (citable) accession number: O35942
Secondary accession number(s): O35959

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents