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O35942

- NEK2_MOUSE

UniProt

O35942 - NEK2_MOUSE

Protein

Serine/threonine-protein kinase Nek2

Gene

Nek2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates and activates NEK11 in G1/S-arrested cells.2 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Its catalytic activity is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371ATPPROSITE-ProRule annotation
    Active sitei141 – 1411Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 229ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. protein kinase activity Source: MGI
    5. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. blastocyst development Source: MGI
    2. centrosome separation Source: Ensembl
    3. chromosome segregation Source: MGI
    4. meiotic nuclear division Source: UniProtKB-KW
    5. mitotic sister chromatid segregation Source: MGI
    6. negative regulation of DNA binding Source: MGI
    7. protein autophosphorylation Source: UniProtKB
    8. protein phosphorylation Source: MGI
    9. regulation of attachment of spindle microtubules to kinetochore Source: UniProtKB
    10. spindle assembly involved in mitosis Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase Nek2 (EC:2.7.11.1)
    Alternative name(s):
    Never in mitosis A-related kinase 2
    Short name:
    NimA-related protein kinase 2
    Gene namesi
    Name:Nek2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:109359. Nek2.

    Subcellular locationi

    Nucleus 1 Publication. Nucleusnucleolus By similarity. Cytoplasm By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle pole By similarity. Chromosomecentromerekinetochore By similarity. Chromosomecentromere 1 Publication
    Note: STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end By similarity.By similarity

    GO - Cellular componenti

    1. centrosome Source: Ensembl
    2. condensed chromosome kinetochore Source: UniProtKB-SubCell
    3. condensed nuclear chromosome Source: MGI
    4. cytoplasm Source: UniProtKB-SubCell
    5. microtubule Source: UniProtKB-KW
    6. midbody Source: MGI
    7. nucleolus Source: UniProtKB-SubCell
    8. spindle pole Source: MGI

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 443443Serine/threonine-protein kinase Nek2PRO_0000086422Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei170 – 1701Phosphothreonine; by autocatalysisBy similarity
    Modified residuei171 – 1711Phosphoserine; by autocatalysisBy similarity
    Modified residuei175 – 1751Phosphothreonine; by autocatalysisBy similarity
    Modified residuei179 – 1791Phosphothreonine; by autocatalysisBy similarity
    Modified residuei241 – 2411Phosphoserine; by autocatalysisBy similarity
    Modified residuei356 – 3561Phosphoserine; by STK3/MST2By similarity
    Modified residuei389 – 3891PhosphoserineBy similarity
    Modified residuei396 – 3961PhosphoserineBy similarity
    Modified residuei401 – 4011PhosphoserineBy similarity
    Modified residuei436 – 4361Phosphoserine; by STK3/MST2By similarity

    Post-translational modificationi

    Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO35942.
    PRIDEiO35942.

    PTM databases

    PhosphoSiteiO35942.

    Expressioni

    Tissue specificityi

    Most abundantly expressed in testis. Low levels found in mid-gestation embryo, ovary, placenta, intestine, thymus and skin. Within the testis, expression restricted to germ cells with highest levels detected in spermatocytes at pachytene and diplotene stages. Also expressed in meiotic pachytene oocytes.2 Publications

    Gene expression databases

    ArrayExpressiO35942.
    BgeeiO35942.
    GenevestigatoriO35942.

    Interactioni

    Subunit structurei

    Forms homodimers and heterodimers. Interacts with CDC20, CTNB1, MAD1L1, MAD2L1, MAPK, NEK11, NPM1, NDC80, PCNT, PPP1CA, PPP1CC and SGOL1. Interacts with STK3/MST2 (via SARAH domain) and SAV1 (via SARAH domain) By similarity. Interacts with NECAB3 and HMGA2.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi201728. 4 interactions.
    IntActiO35942. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliO35942.
    SMRiO35942. Positions 3-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 271264Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni264 – 443180Interaction with PCNTBy similarityAdd
    BLAST
    Regioni306 – 33429Leucine-zipperAdd
    BLAST
    Regioni329 – 443115Necessary for interaction with MAD1L1By similarityAdd
    BLAST
    Regioni333 – 37038Required for microtubule binding and for localization to the centrosomesBy similarityAdd
    BLAST
    Regioni402 – 43736Interaction with SAV1 and STK3/MST2By similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili303 – 36159Sequence AnalysisAdd
    BLAST
    Coiled coili403 – 42725Sequence AnalysisAdd
    BLAST

    Domaini

    The leucine-zipper domain is required for its dimerization and activation.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00720000108402.
    HOGENOMiHOG000233029.
    HOVERGENiHBG006461.
    InParanoidiQ3TPD7.
    KOiK08857.
    OMAiSKCKDLK.
    OrthoDBiEOG715Q40.
    TreeFamiTF101184.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35942-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSRVEDYEV LHSIGTGSYG RCQKIRRKSD GKILVWKELD YGSMTEVEKQ    50
    MLVSEVNLLR ELKHPNIVSY YDRIIDRTNT TLYIVMEYCE GGDLASVISK 100
    GTKDRQYLEE EFVLRVMTQL TLALKECHRR SDGGHTVLHR DLKPANVFLD 150
    SKHNVKLGDF GLARILNHDT SFAKTFVGTP YYMSPEQMSC LSYNEKSDIW 200
    SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR 250
    MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLPDSS 300
    PVLSELKLKE RQLQDREQAL RAREDILEQK ERELCIRERL AEDKLARAES 350
    LMKNYSLLKE HRLLCLAGGP ELDLPSSAMK KKVHFHGESK ENTARSENSE 400
    SYLAKSKCRD LKKRLHAAQL RAQALADIEK NYQLKSRQIL GMR 443
    Length:443
    Mass (Da):51,243
    Last modified:July 27, 2011 - v2
    Checksum:i1EF2CA320F60FB5C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti69 – 691S → R in AAB67973. (PubMed:9187143)Curated
    Sequence conflicti69 – 691S → R in AAC35393. (PubMed:9583679)Curated
    Sequence conflicti69 – 691S → R in AAB70470. (PubMed:9434622)Curated
    Sequence conflicti203 – 2031G → A in AAB67973. (PubMed:9187143)Curated
    Sequence conflicti253 – 2531N → F in AAB67973. (PubMed:9187143)Curated
    Sequence conflicti274 – 2741L → M in AAB67973. (PubMed:9187143)Curated
    Sequence conflicti274 – 2741L → M in AAC35393. (PubMed:9583679)Curated
    Sequence conflicti274 – 2741L → M in AAB70470. (PubMed:9434622)Curated
    Sequence conflicti311 – 3111R → S in AAB67973. (PubMed:9187143)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U95610 mRNA. Translation: AAB67973.1.
    AF013166 mRNA. Translation: AAC35393.1.
    AF007247 mRNA. Translation: AAB70470.1.
    AK147072 mRNA. Translation: BAE27653.1.
    AK164467 mRNA. Translation: BAE37799.1.
    CCDSiCCDS15623.1.
    RefSeqiNP_035022.2. NM_010892.3.
    UniGeneiMm.33773.
    Mm.403999.

    Genome annotation databases

    EnsembliENSMUST00000027931; ENSMUSP00000027931; ENSMUSG00000026622.
    GeneIDi18005.
    KEGGimmu:18005.
    UCSCiuc007ecx.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U95610 mRNA. Translation: AAB67973.1 .
    AF013166 mRNA. Translation: AAC35393.1 .
    AF007247 mRNA. Translation: AAB70470.1 .
    AK147072 mRNA. Translation: BAE27653.1 .
    AK164467 mRNA. Translation: BAE37799.1 .
    CCDSi CCDS15623.1.
    RefSeqi NP_035022.2. NM_010892.3.
    UniGenei Mm.33773.
    Mm.403999.

    3D structure databases

    ProteinModelPortali O35942.
    SMRi O35942. Positions 3-314.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201728. 4 interactions.
    IntActi O35942. 3 interactions.

    PTM databases

    PhosphoSitei O35942.

    Proteomic databases

    PaxDbi O35942.
    PRIDEi O35942.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027931 ; ENSMUSP00000027931 ; ENSMUSG00000026622 .
    GeneIDi 18005.
    KEGGi mmu:18005.
    UCSCi uc007ecx.2. mouse.

    Organism-specific databases

    CTDi 4751.
    MGIi MGI:109359. Nek2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00720000108402.
    HOGENOMi HOG000233029.
    HOVERGENi HBG006461.
    InParanoidi Q3TPD7.
    KOi K08857.
    OMAi SKCKDLK.
    OrthoDBi EOG715Q40.
    TreeFami TF101184.

    Enzyme and pathway databases

    Reactomei REACT_196635. Regulation of PLK1 Activity at G2/M Transition.

    Miscellaneous databases

    NextBioi 293015.
    PROi O35942.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35942.
    Bgeei O35942.
    Genevestigatori O35942.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The NIMA-related kinase 2, Nek2, is expressed in specific stages of the meiotic cell cycle and associates with meiotic chromosomes."
      Rhee K., Wolgemuth D.J.
      Development 124:2167-2177(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Swiss Webster.
      Tissue: Testis.
    2. "Murine NIMA-related kinases are expressed in patterns suggesting distinct functions in gametogenesis and a role in the nervous system."
      Arama E., Yanai A., Kilfin G., Motro B.
      Oncogene 16:1813-1823(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis."
      Tanaka K., Parvinen M., Nigg E.A.
      Exp. Cell Res. 237:264-274(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Brain.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye and Heart.
    5. "NIP1/XB51/NECAB3 is a potential substrate of Nek2, suggesting specific roles of Nek2 in Golgi."
      Yoo J.C., Chang J.R., Kim S.H., Jang S.K., Wolgemuth D.J., Kim K., Rhee K.
      Exp. Cell Res. 292:393-402(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NECAB3.
    6. "Phosphorylation of high-mobility group protein A2 by Nek2 kinase during the first meiotic division in mouse spermatocytes."
      Di Agostino S., Fedele M., Chieffi P., Fusco A., Rossi P., Geremia R., Sette C.
      Mol. Biol. Cell 15:1224-1232(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HMGA2.

    Entry informationi

    Entry nameiNEK2_MOUSE
    AccessioniPrimary (citable) accession number: O35942
    Secondary accession number(s): O35959, Q3TPD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3