O35932 (PD2RL_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostaglandin D2 receptor-like
Short name=PGD receptor-like
Short name=PGD2 receptor-like

Alternative name(s):
Prostanoid DP receptor-like

Gene names

Name:	Ptgdrl
Synonyms:	Ptgdr2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Receptor for prostaglandin D2 (PGD2). The activity of this receptor is mainly mediated by G(s) proteins that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP. A mobilization of calcium is also observed, but without formation of inositol 1,4,5-trisphosphate By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Tissue specificity	Strongly expressed in eye and gastrointestinal tract (GIT), moderately in the brain and oviduct and weakly in the epididymis. In the eye, expressed in the epithelium of the iris and ciliary body and in photoreceptor cells of the retina. In the brain, expressed in leptomeninges, choroid plexus and spinal cord (sensory and motor neurons of the dorsal and ventral horns). In the stomach, expressed in the mucous-secreting goblet cells and the columnar epithelium. Expressed in platelets. Ref.1 Ref.2
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytosolic calcium ion homeostasis Inferred from mutant phenotype PubMed 19576888. Source: RGD positive regulation of penile erection Inferred from direct assay PubMed 18047485. Source: RGD positive regulation of vasodilation Inferred from mutant phenotype PubMed 17643322. Source: RGD
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay PubMed 10448933. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	prostaglandin D receptor activity Inferred from direct assay PubMed 10448933. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Prostaglandin D2 receptor-like		PRO_0000370712
Regions
	Topological domain	1 – 20	20	Extracellular Potential
	Transmembrane	21 – 41	21	Helical; Name=1; Potential
	Topological domain	42 – 57	16	Cytoplasmic Potential
	Transmembrane	58 – 78	21	Helical; Name=2; Potential
	Topological domain	79 – 106	28	Extracellular Potential
	Transmembrane	107 – 127	21	Helical; Name=3; Potential
	Topological domain	128 – 149	22	Cytoplasmic Potential
	Transmembrane	150 – 170	21	Helical; Name=4; Potential
	Topological domain	171 – 194	24	Extracellular Potential
	Transmembrane	195 – 215	21	Helical; Name=5; Potential
	Topological domain	216 – 261	46	Cytoplasmic Potential
	Transmembrane	262 – 282	21	Helical; Name=6; Potential
	Topological domain	283 – 306	24	Extracellular Potential
	Transmembrane	307 – 327	21	Helical; Name=7; Potential
	Topological domain	328 – 357	30	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	2	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 182		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35932 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: DB966276DD68184C	FASTA	357	39,803
	10 20 30 40 50 60 MNESYRCQAA TWVERGSSAT MGGVLFSAGL LGNLLALVLL ARSGLGSCRP GPLHPPPSVF 70 80 90 100 110 120 YVLVCGLTVT HLLGKCLISP MVLAAYAQNR SLKELLPASG NQLCEAFAFL MSFFGLASTL 130 140 150 160 170 180 QLLAMALECW LSLGHPFFYQ RHITARRGVL VAPVAGAFSL AFCALPFAGF GKFVQYCPGT 190 200 210 220 230 240 WCFIQMIHKK RSFSVIGFSV LYSSLMALLV LATVVCNLGA MSNLYAMHRR QRHHPRRCSR 250 260 270 280 290 300 DRAQSGSDYR HGSPNPLEEL DHFVLLALTT VLFTMCSLPL IYRAYYGAFK LVDRADGDSE 310 320 330 340 350 DLQALRFLSV ISIVDPWIFI IFRTSVFRML FHKAFTRPLI YRNWCSHSWQ TNMESTL

References

[1]	"Dominant expression of rat prostanoid DP receptor mRNA in leptomeninges, inner segments of photoreceptor cells, iris epithelium, and ciliary processes." Gerashchenko D., Beuckmann C.T., Kanaoka Y., Eguchi N., Gordon W.C., Urade Y., Bazan N.G., Hayaishi O. J. Neurochem. 71:937-945(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Sprague-Dawley. Tissue: Retina.
[2]	"Pharmacological and cardiovascular properties of a hydantoin derivative, BW 245 C, with high affinity and selectivity for PGD2 receptors." Town M.H., Casals-Stenzel J., Schillinger E. Prostaglandins 25:13-28(1983) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U92289 mRNA. Translation: AAB71762.1.
IPI	IPI00326706.
RefSeq	NP_071577.1. NM_022241.1.
UniGene	Rn.110861.
3D structure databases
ProteinModelPortal	O35932.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000048101.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	O35932.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	63889.
KEGG	rno:63889.
Organism-specific databases
CTD	63889.
RGD	619707. Ptgdrl.
Phylogenomic databases
eggNOG	NOG248831.
HOGENOM	HOG000115475.
InParanoid	O35932.
KO	K04332.
OrthoDB	EOG41JZCX.
Gene expression databases
ArrayExpress	O35932.
Genevestigator	O35932.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR000376. Pglndn_D_rcpt. IPR008365. Prostanoid_rcpt. [Graphical view]
PANTHER	PTHR11866. PTHR11866. 1 hit. PTHR11866:SF9. PTHR11866:SF9. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR01788. PROSTANOIDR. PR00854. PRSTNOIDDPR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. False negative. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	612534.

Entry information

Entry name

PD2RL_RAT

Accession

Primary (citable) accession number: O35932

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program