O35927 (CTND2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catenin delta-2

Alternative name(s):
Neural plakophilin-related ARM-repeat protein
Short name=NPRAP
Neurojungin

Gene names

Name:	Ctnnd2
Synonyms:	Catnd2, Nprap

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1247 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules. Functions as a transcriptional activator when bound to ZBTB33. Ref.3 Ref.6
Subunit structure	Binds to E-cadherin at a juxtamembrane site within the cytoplasmic domain. Binds to PSEN1. Interacts with PDZD2. Interacts (via the extreme C-terminus) with FRMPD2 (via the PDZ 2 domain) By similarity. Interacts with ZBTB33. Interacts with ARHGEF28. Ref.3 Ref.6 Ref.13
Subcellular location	Nucleus. Cell junction › adherens junction. Note: Co-localizes with CDK5 in the cell body of neuronal cells By similarity. Ref.5 Ref.6
Tissue specificity	Expressed in neurons and glial cells. Isoform 2 was found to be the most predominant isoform in various brain regions. Expressed at neuromuscular junctions. Ref.5 Ref.6
Developmental stage	Increasingly expressed by embryonic day E10. Expression peaks at postnatal day P7 and stays at lower levels in adulthood. First expressed within proliferating neuronal progenitor cells of the neuroepithelium, becomes down-regulated during neuronal migration, and is later reexpressed in the dendritic compartment of postmitotic neurons. In the developing neocortex, it is strongly expressed in the proliferative ventricular zone and the developing cortical plate, yet is conspicuously less prominent in the intermediate zone, which contains migrating cortical neurons, it forms a honeycomb pattern in the neuroepithelium by labeling the cell periphery in a typical adherens junction pattern. By E18, its expression shifts primarily to nascent apical dendrites, a pattern that continues through adulthood.
Induction	By retinoic acid. Ref.4
Post-translational modification	O-glycosylated. Phosphorylated by CDK5 By similarity.
Sequence similarities	Belongs to the beta-catenin family. Contains 9 ARM repeats.

Ontologies

Keywords
Biological process	Cell adhesion Transcription Transcription regulation
Cellular component	Cell junction Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat
Molecular function	Developmental protein
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW learning Inferred from mutant phenotype PubMed 15380068. Source: MGI morphogenesis of a branching structure Inferred from direct assay PubMed 17097608. Source: MGI multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW regulation of synaptic plasticity Inferred from mutant phenotype PubMed 15380068. Source: MGI regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	adherens junction Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic density Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O35927-1)

Also known as: NPRAPa;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O35927-2)

Also known as: NPRAPb;

The sequence of this isoform differs from the canonical sequence as follows:
     878-902: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1247	1247	Catenin delta-2		PRO_0000064300
Regions
	Repeat	391 – 433	43	ARM 1
	Repeat	537 – 576	40	ARM 2
	Repeat	579 – 618	40	ARM 3
	Repeat	623 – 663	41	ARM 4
	Repeat	679 – 721	43	ARM 5
	Repeat	725 – 770	46	ARM 6
	Repeat	832 – 872	41	ARM 7
	Repeat	904 – 943	40	ARM 8
	Repeat	997 – 1040	44	ARM 9
	Coiled coil	49 – 84	36	Potential
	Compositional bias	218 – 225	8	Poly-Pro
	Compositional bias	245 – 248	4	Poly-Ala
	Compositional bias	478 – 481	4	Poly-Ala
	Compositional bias	808 – 814	7	Poly-Lys
Amino acid modifications
	Modified residue	32	1	Phosphoserine Ref.7
	Modified residue	264	1	Phosphoserine Ref.12
	Modified residue	273	1	Phosphoserine Ref.12
	Modified residue	412	1	Phosphoserine Ref.8 Ref.10
	Modified residue	471	1	Phosphoserine Ref.12
	Modified residue	513	1	Phosphotyrosine Ref.11
	Modified residue	531	1	Phosphoserine Ref.8
	Modified residue	534	1	Phosphoserine Ref.8
	Modified residue	1094	1	Phosphoserine Ref.10
	Modified residue	1098	1	Phosphoserine Ref.10
	Modified residue	1149	1	Phosphoserine Ref.12
Natural variations
	Alternative sequence	878 – 902	25	Missing in isoform 2.		VSP_006747

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (NPRAPa) [UniParc]. Last modified January 1, 1998. Version 1. Checksum: D4A7A6B6A27D2919	FASTA	1,247	135,000
	10 20 30 40 50 60 MFARKQSGAA PFGAMPVPDQ PPSASEKNSS LSPGLNTSNG DGSETETTSA ILASVKEQEL 70 80 90 100 110 120 QFERLTRELE AERQIVASQL ERCKLGSETG SMSSISSAGE QFHWQTQDGQ KDIEDELTTG 130 140 150 160 170 180 LELVDSCIRS LQESGILDPQ DYSTSERPSL LSQSALQLNS KPEGSFQYPA SYHSNQTLAL 190 200 210 220 230 240 GDTAPSQLPA RSTQARAAGQ SFSQGTTGRA GHLAGSEPAP PPPPPREPFA PSLGSAFHLP 250 260 270 280 290 300 DAPPAAAALY YSSSTLPAPP RGGSPLTTTQ GGSPTKLQRG GSAPEGAAYA APRGSSPKQS 310 320 330 340 350 360 PSRLAKSYST SSPINIVVSS AGLSPIRVTS PPTVQSTISS SPIHQLSSTI GTYATLSPTK 370 380 390 400 410 420 RLVHASEQYS KHSQELYATA TLQRPGSLAA GSRASYSSQH GHLAPELRAL QSPEHHIDPI 430 440 450 460 470 480 YEDRVYQKPP MRSLSQSQGD PLPPAHTGTF RTSTAPSSPG VDSVPLQRTG SQHGPQNAAA 490 500 510 520 530 540 ATFQRASYAA GPASNYADPY RQLQYCASVD SPYSKSGPAL PPEGTLARSP SIDSIQKDPR 550 560 570 580 590 600 EFGWRDPELP EVIQMLQHQF PSVQSNAAAY LQHLCFGDNK IKAEIRRQGG IQLLVDLLDH 610 620 630 640 650 660 RMTEVHRSAC GALRNLVYGK ANDDNKIALK NCGGIPALVR LLRKTTDLEI RELVTGVLWN 670 680 690 700 710 720 LSSCDALKMP IIQDALAVLT NAVIIPHSGW ENSPLQDDRK IQLHSSQVLR NATGCLRNVS 730 740 750 760 770 780 SAGEEARRRM RECDGLTDAL LYVIQSALGS SEIDSKTVEN CVCILRNLSY RLAAETSQGQ 790 800 810 820 830 840 HMGTDELDGL LCGETNGKDT ESSGCWGKKK KKKKSQDQWD GVGPLPDCAE PPKGIQMLWH 850 860 870 880 890 900 PSIVKPYLTL LSECSNPDTL EGAAGALQNL AAGSWKGWAE DVAGMAYALR SLPEGAPCLP 910 920 930 940 950 960 QWSVYIRAAV RKEKGLPILV ELLRIDNDRV VCAVATALRN MALDVRNKEL IGKYAMRDLV 970 980 990 1000 1010 1020 HRLPGGNNSN NSGSKAMSDD TVTAVCCTLH EVITKNMENA KALRDAGGIE KLVGISKSKG 1030 1040 1050 1060 1070 1080 DKHSPKVVKA ASQVLNSMWQ YRDLRSLYKK DGWSQYHFVA SSSTIERDRQ RPYSSSRTPS 1090 1100 1110 1120 1130 1140 ISPVRVSPNN RSASAPASPR EMISLKERKT DYESAGNNAT YHGTKGEHTS RKDTMTAQNT 1150 1160 1170 1180 1190 1200 GVSTLYRNSY GAPAEDIKQN QVSTQPVPQE PSRKDYETYQ PFPNSTRNYD ESFFEDQVHH 1210 1220 1230 1240 RPPASEYTMH LGLKSTGNYV DFYSAARPYS ELNYETSHYP ASPDSWV
	Isoform 2 (NPRAPb) [UniParc]. Checksum: 9570D985F604C41B	FASTA	1,222	132,286
	10 20 30 40 50 60 MFARKQSGAA PFGAMPVPDQ PPSASEKNSS LSPGLNTSNG DGSETETTSA ILASVKEQEL 70 80 90 100 110 120 QFERLTRELE AERQIVASQL ERCKLGSETG SMSSISSAGE QFHWQTQDGQ KDIEDELTTG 130 140 150 160 170 180 LELVDSCIRS LQESGILDPQ DYSTSERPSL LSQSALQLNS KPEGSFQYPA SYHSNQTLAL 190 200 210 220 230 240 GDTAPSQLPA RSTQARAAGQ SFSQGTTGRA GHLAGSEPAP PPPPPREPFA PSLGSAFHLP 250 260 270 280 290 300 DAPPAAAALY YSSSTLPAPP RGGSPLTTTQ GGSPTKLQRG GSAPEGAAYA APRGSSPKQS 310 320 330 340 350 360 PSRLAKSYST SSPINIVVSS AGLSPIRVTS PPTVQSTISS SPIHQLSSTI GTYATLSPTK 370 380 390 400 410 420 RLVHASEQYS KHSQELYATA TLQRPGSLAA GSRASYSSQH GHLAPELRAL QSPEHHIDPI 430 440 450 460 470 480 YEDRVYQKPP MRSLSQSQGD PLPPAHTGTF RTSTAPSSPG VDSVPLQRTG SQHGPQNAAA 490 500 510 520 530 540 ATFQRASYAA GPASNYADPY RQLQYCASVD SPYSKSGPAL PPEGTLARSP SIDSIQKDPR 550 560 570 580 590 600 EFGWRDPELP EVIQMLQHQF PSVQSNAAAY LQHLCFGDNK IKAEIRRQGG IQLLVDLLDH 610 620 630 640 650 660 RMTEVHRSAC GALRNLVYGK ANDDNKIALK NCGGIPALVR LLRKTTDLEI RELVTGVLWN 670 680 690 700 710 720 LSSCDALKMP IIQDALAVLT NAVIIPHSGW ENSPLQDDRK IQLHSSQVLR NATGCLRNVS 730 740 750 760 770 780 SAGEEARRRM RECDGLTDAL LYVIQSALGS SEIDSKTVEN CVCILRNLSY RLAAETSQGQ 790 800 810 820 830 840 HMGTDELDGL LCGETNGKDT ESSGCWGKKK KKKKSQDQWD GVGPLPDCAE PPKGIQMLWH 850 860 870 880 890 900 PSIVKPYLTL LSECSNPDTL EGAAGALQNL AAGSWKGSVY IRAAVRKEKG LPILVELLRI 910 920 930 940 950 960 DNDRVVCAVA TALRNMALDV RNKELIGKYA MRDLVHRLPG GNNSNNSGSK AMSDDTVTAV 970 980 990 1000 1010 1020 CCTLHEVITK NMENAKALRD AGGIEKLVGI SKSKGDKHSP KVVKAASQVL NSMWQYRDLR 1030 1040 1050 1060 1070 1080 SLYKKDGWSQ YHFVASSSTI ERDRQRPYSS SRTPSISPVR VSPNNRSASA PASPREMISL 1090 1100 1110 1120 1130 1140 KERKTDYESA GNNATYHGTK GEHTSRKDTM TAQNTGVSTL YRNSYGAPAE DIKQNQVSTQ 1150 1160 1170 1180 1190 1200 PVPQEPSRKD YETYQPFPNS TRNYDESFFE DQVHHRPPAS EYTMHLGLKS TGNYVDFYSA 1210 1220 ARPYSELNYE TSHYPASPDS WV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and localization of a neurally expressed member of the plakoglobin/armadillo multigene family." Paffenholz R., Franke W.W. Differentiation 61:293-304(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING. Tissue: Fetal brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[3]	"Delta-catenin, an adhesive junction-associated protein which promotes cell scattering." Lu Q., Paredes M., Medina M., Zhou J., Cavallo R., Peifer M., Orecchio L., Kosik K.S. J. Cell Biol. 144:519-532(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH E-CADHERIN. Tissue: Brain.
[4]	"Expression of the mRNA for two isoforms of neural plakophilin-related arm-repeat protein/delta-catenin in rodent neurons and glial cells." Kawamura Y., Fan Q.W., Hayashi H., Michikawa M., Yanagisawa K., Komano H. Neurosci. Lett. 277:185-188(1999) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS 1 AND 2), INDUCTION.
[5]	"Delta-catenin is a nervous system-specific adherens junction protein which undergoes dynamic relocalization during development." Ho C., Zhou J., Medina M., Goto T., Jacobson M., Bhide P.G., Kosik K.S. J. Comp. Neurol. 420:261-276(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[6]	"Regulation of the rapsyn promoter by kaiso and delta-catenin." Rodova M., Kelly K.F., VanSaun M., Daniel J.M., Werle M.J. Mol. Cell. Biol. 24:7188-7196(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ZBTB33, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, MASS SPECTROMETRY. Tissue: Embryonic brain.
[8]	"Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412; SER-531 AND SER-534, MASS SPECTROMETRY. Tissue: Forebrain.
[9]	"O-linked N-acetylglucosamine proteomics of postsynaptic density preparations using lectin weak affinity chromatography and mass spectrometry." Vosseller K., Trinidad J.C., Chalkley R.J., Specht C.G., Thalhammer A., Lynn A.J., Snedecor J.O., Guan S., Medzihradszky K.F., Maltby D.A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:923-934(2006) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Tissue: Brain.
[10]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412; SER-1094 AND SER-1098, MASS SPECTROMETRY. Tissue: Brain.
[11]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-513, MASS SPECTROMETRY. Tissue: Brain.
[12]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264; SER-273; SER-471 AND SER-1149, MASS SPECTROMETRY. Tissue: Brain cortex.
[13]	"Delta-catenin-induced dendritic morphogenesis. An essential role of p190RhoGEF interaction through Akt1-mediated phosphorylation." Kim H., Han J.-R., Park J., Oh M., James S.E., Chang S., Lu Q., Lee K.Y., Ki H., Song W.-J., Kim K. J. Biol. Chem. 283:977-987(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARHGEF28.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U90331 mRNA. Translation: AAB82409.1. BC138260 mRNA. Translation: AAI38261.1. BC138261 mRNA. Translation: AAI38262.1.
IPI	IPI00136135. IPI00228632.
PIR	T42209.
RefSeq	NP_032755.2. NM_008729.2.
UniGene	Mm.321648.
3D structure databases
ProteinModelPortal	O35927.
SMR	O35927. Positions 539-1058.
ModBase	Search...
Protein-protein interaction databases
IntAct	O35927. 3 interactions.
MINT	MINT-4092555.
PTM databases
PhosphoSite	O35927.
Proteomic databases
PaxDb	O35927.
PRIDE	O35927.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	18163.
KEGG	mmu:18163.
Organism-specific databases
CTD	1501.
MGI	MGI:1195966. Ctnnd2.
Phylogenomic databases
eggNOG	NOG276924.
HOGENOM	HOG000231863.
HOVERGEN	HBG004284.
OrthoDB	EOG4S4PFC.
Gene expression databases
ArrayExpress	O35927.
Bgee	O35927.
CleanEx	MM_CTNND2.
Genevestigator	O35927.
GermOnline	ENSMUSG00000022240. Mus musculus.
Family and domain databases
Gene3D	1.25.10.10. 2 hits.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. [Graphical view]
Pfam	PF00514. Arm. 4 hits. [Graphical view]
SMART	SM00185. ARM. 7 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50176. ARM_REPEAT. 3 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CTNND2. mouse.
NextBio	293442.
SOURCE	Search...

Entry information

Entry name

CTND2_MOUSE

Accession

Primary (citable) accession number: O35927
Secondary accession number(s): B2RR80

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	January 1, 1998
Last modified:	May 29, 2013
This is version 117 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program