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O35923 (BRCA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Breast cancer type 2 susceptibility protein homolog
Alternative name(s):
Fanconi anemia group D1 protein homolog
Gene names
Name:Brca2
Synonyms:Fancd1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length3343 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication By similarity.

Subunit structure

Monomer and dimer. Interacts with RAD51; regulates RAD51 recruitment and function at sites of DNA repair. Interacts with DSS1, WDR16, USP11, DMC1, ROCK2 and NPM1. Interacts with both nonubiquitinated and monoubiquitinated FANCD2; this complex also includes XRCC3 and phosphorylated FANCG. Part of a BRCA complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2 which may serve as a scaffold for a HR complex containing PALB2, BRCA2, RAD51C, RAD51 and XRCC3. Interacts with BRCA1 only in the presence of PALB2 which serves as the bridging protein. Interacts with POLH; the interaction is direct.

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Nucleus By similarity.

Tissue specificity

Highest expression in testis. Also expressed in spleen, skeletal muscle, thymus, mammary gland, heart, ovary, prostate, liver, lung, kidney and brain.

Post-translational modification

Phosphorylation by CHEK1 and CHEK2 regulates interaction with RAD51. Phosphorylation at Ser-3222 by CDK1 and CDK2 is low in S phase when recombination is active, but increases as cells progress towards mitosis; this phosphorylation prevents homologous recombination-dependent repair during S phase and G2 by inhibiting RAD51 binding By similarity.

Ubiquitinated in the absence of DNA damage; this does not lead to proteasomal degradation. In contrast, ubiquitination in response to DNA damage leads to proteasomal degradation By similarity.

Sequence similarities

Contains 8 BRCA2 repeats.

Ontologies

Keywords
   Biological processCell cycle
DNA damage
DNA recombination
DNA repair
   Cellular componentCytoplasm
Cytoskeleton
Nucleus
   DiseaseTumor suppressor
   DomainRepeat
   LigandDNA-binding
   PTMPhosphoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA recombination

Inferred from direct assay Ref.2. Source: RGD

centrosome duplication

Inferred from Biological aspect of Ancestor. Source: RefGenome

double-strand break repair via homologous recombination

Inferred from sequence or structural similarity. Source: UniProtKB

homologous chromosome orientation involved in meiotic metaphase I plate congression

Inferred from mutant phenotype PubMed 16964288. Source: RGD

mammary gland development

Inferred from mutant phenotype PubMed 16964288. Source: RGD

multicellular organism growth

Inferred from mutant phenotype PubMed 16964288. Source: RGD

regulation of cell proliferation

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to estradiol

Inferred from expression pattern PubMed 14583453. Source: RGD

response to nutrient

Inferred from expression pattern PubMed 14583453. Source: RGD

spermatogenesis

Inferred from mutant phenotype PubMed 16964288. Source: RGD

   Cellular_componentcentrosome

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functiongamma-tubulin binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

single-stranded DNA binding

Inferred from direct assay Ref.2. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 33433343Breast cancer type 2 susceptibility protein homolog
PRO_0000064986

Regions

Repeat984 – 101835BRCA2 1
Repeat1197 – 123135BRCA2 2
Repeat1405 – 143935BRCA2 3
Repeat1503 – 153735BRCA2 4
Repeat1645 – 166925BRCA2 5
Repeat1828 – 184518BRCA2 6
Repeat1939 – 197335BRCA2 7
Repeat2019 – 205335BRCA2 8
Region1 – 4040Interaction with PALB2 By similarity
Region622 – 982361Interaction with NPM1 By similarity
Region2313 – 2475163Interaction with FANCD2 By similarity

Amino acid modifications

Modified residue7361Phosphoserine By similarity
Modified residue32221Phosphoserine; by CDK1 and CDK2 By similarity

Secondary structure

.......................................................................................................... 3343
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O35923 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 653DB110D2302A8D

FASTA3,343372,216
        10         20         30         40         50         60 
MTVEYKRRPT FWEIFKARCS TADLGPISLN WFEELFSEAP PYNTEHPEES EYKPQGHEPQ 

        70         80         90        100        110        120 
LFKTPQRNPS YHQFASTPIM FKEQSQTLPL DQSPFKELGN VVANSKRKHH SKKKARKDPV 

       130        140        150        160        170        180 
VDVASLPLKA CPSESPCTPR CTQVAPQRRK PVVSGSLFYT PKLEETPKHI SESLGVEVDP 

       190        200        210        220        230        240 
DMSWTSSLAT PPTLSSTVLI ARDEEAHRNA FPADSPASLK SYFSNHNESL KKNDRFIPSV 

       250        260        270        280        290        300 
SDSENKSQQE AFSQGLEKML GDSSSKINRF RDCLRKPIPN VLEDGETAVD TSGEDSFSLC 

       310        320        330        340        350        360 
FPKRRTRNLQ KTRMGKMKKK IFSETRTDGL SEEARGQADD KNSFALEIEP RDSEPLDPSV 

       370        380        390        400        410        420 
TNQKPLYSQS GDISSEAGQC SDSIWSQPDP SGLNGTQTRK IPLLHISFHK QSILEDFIDM 

       430        440        450        460        470        480 
KKEGTGSITF PHISSLPEPE KMFSEETLVD KEHEGQHLES LEDSISGKQM VSGTSQTACL 

       490        500        510        520        530        540 
SPSIRKSIVK MREPLEETLD TVFSDSMTSS AFTEELDASA GGLEIHTACS QREDSLCPSS 

       550        560        570        580        590        600 
VDTGSWPTTL TDTSATVKNA GLITTLKNKR RKFIYSVSDD ASHQGKKLQT QRQSELTNLS 

       610        620        630        640        650        660 
APFEASAFEV PFPFTNVDSG IPDSSIKRSN LPNDPEEPSL SLTNSFVTAA SKEISYIHAL 

       670        680        690        700        710        720 
ISQDLNDKEA ILSEEKPQPY TALEADFLSC LPERSCENDQ KSPKVSDRKE KVLVSACRPS 

       730        740        750        760        770        780 
GRLAAAVQLS SISFDSQENP LGSHNVTSTL KLTPSPKTPL SKPVVVSRGK MCKMPEKLQC 

       790        800        810        820        830        840 
KSCKDNIELS KNIPLGVNEM CVLSENSETP ELLPPLEYIT EVSSSVKSQF NQNTKIAVVQ 

       850        860        870        880        890        900 
KDQKDSTFIS EVTVHMNSEE LFPEKENNFA FQVTNESNKP NIGSTVEFQE EDLSHAKGHS 

       910        920        930        940        950        960 
LKNSPMTVDR DLDDEQAGQV LITEDSDSLA VVHDCTKKSR NTIEQHQKGT ADKDFKSNSS 

       970        980        990       1000       1010       1020 
LYLKSDGNND YLDKWSEFLD PLMNHKLGGS FRTASNKEIK LSEDNVKKSK MFFKDIEEQY 

      1030       1040       1050       1060       1070       1080 
PTSLDCIDTV STLQLANKKR LSEPHTFDLK SGTTVSTQCH SQSSVSHEDT HTAPQMLSSK 

      1090       1100       1110       1120       1130       1140 
QDFHSSHNLT PSQKAEITEL STILEESGSQ FEFTQFKNPS HIAQNNTSAV LGNQMAVVRT 

      1150       1160       1170       1180       1190       1200 
ASEEWKDVDL HLPLNPSSVG QIDHNKKFEC LVGVKQSSSH LLEDTCNQNT SCFLPIKEME 

      1210       1220       1230       1240       1250       1260 
FGGFCSALGT KLSVSNEALR KAMKLFSDIE NISEEPSTKV GPRGFSSCAH HDSVASVFKI 

      1270       1280       1290       1300       1310       1320 
KKQNTDKSFD EKSSKCQVTV QNNKEMTTCI LVDENPENYV KNIKQDNNYT GSQRNAYKLE 

      1330       1340       1350       1360       1370       1380 
NSDVSKSSTS GTVYINKGDS DLPFAAEKGN KYPESCTQYV REENAQIKES VSDLTCLEVM 

      1390       1400       1410       1420       1430       1440 
KAEETCHMKS SDKEQLPSDK MEQNMKEFNI SFQTASGKNI RVSKESLNKS VNILDQETED 

      1450       1460       1470       1480       1490       1500 
LTVTSDSLNS KILCGINKDK MHISCHKKSI NIKKVFEEHF PIGTVSQLPA LQQYPEYEIE 

      1510       1520       1530       1540       1550       1560 
SIKEPTLLSF HTASGKKVKI MQESLDKVKN LFDETQYVRK TTNFGHQESK PLKDREDYKE 

      1570       1580       1590       1600       1610       1620 
RLTLAYEKIE VTASKCEEMQ NFVSKQTEML PQQNDHMYRQ TENLTSNGSS PKVHGNIENK 

      1630       1640       1650       1660       1670       1680 
IEKNPRICCI CQSSYFVTED SALACYTGDS RKTCVGESSL SKGKKWLREQ SDKLGTRNTI 

      1690       1700       1710       1720       1730       1740 
EIQCVKEHTE DFAGNALYEH SLVIIRTEID TSHVSENQAS TLFSDPNVCH SYLSHSSFCH 

      1750       1760       1770       1780       1790       1800 
HDDMHNDSGY FLKDKIDSDV QPDMKNTEGN AIFPKISATK EIKLHPQTVN EECVQKLETN 

      1810       1820       1830       1840       1850       1860 
ASPYANKNIA IDSAMLDLRN CKVGSPVFIT THSQETVRMK EIFTDNCSKI VEQNRESKPD 

      1870       1880       1890       1900       1910       1920 
TCQTSCHKAL DNSEDFICPS SSGDVCINSP MAIFYPQSEQ ILQHNQSVSG LKKAATPPVS 

      1930       1940       1950       1960       1970       1980 
LETWDTCKSI RGSPQEVHPS RTYGFFSTAS GKAVQVSDAS LEKARQVFSE IDGDAKQLAS 

      1990       2000       2010       2020       2030       2040 
MVSLEGNEKS HHSVKRESSV VHNTHGVLSL RKTLPGNVSS FVFSGFSTAG GKLVTVSESA 

      2050       2060       2070       2080       2090       2100 
LHKVKGMLEE FDLIRTEHTL QHSPTPEDVS KIPPQPCLES RTPEYSVSSK LQKTYNDKSR 

      2110       2120       2130       2140       2150       2160 
SPSNYKESGS SGNTQSLEVS PQLSQMERKQ ETQSVLGTKV SQRKTNILEK KQNLPQNIKI 

      2170       2180       2190       2200       2210       2220 
ESNKMETFSD VSMKTNVGEY YSKEPENYFE TEAVEIAKAF MEDDELTDSE QTHAKCSLFA 

      2230       2240       2250       2260       2270       2280 
CPQNEALLNS RTRKRGGMAG VAVGQPPIKR SLLNEFDRII ESKGKSLTPS KSTPDGTIKD 

      2290       2300       2310       2320       2330       2340 
RRLFTHHMSL EPVTCGPFCS SKERQETQSP HVTSPAQGLQ SKEHPSRHSA VGKSSSNPTV 

      2350       2360       2370       2380       2390       2400 
SALRSERTRH SVSDKSTKVF VPPFKVKSRF HRDEHFDSKN VNLEGKNQKS ADGVSEDGND 

      2410       2420       2430       2440       2450       2460 
SDFPQFNKDL MSSLQNARDL QDIRIKNKER HHLCPQPGSL YLTKSSTLPR ISLQAAVGDS 

      2470       2480       2490       2500       2510       2520 
VPSACSPKQL YMYGVSKACI SVNSKNAEYF QFAIEDHFGK EALCAGKGFR LADGGWLIPS 

      2530       2540       2550       2560       2570       2580 
DDGKAGKEEF YRALCDTPGV DPKLISSVWV SNHYRWIVWK LAAMEFAFPK EFANRCLNPE 

      2590       2600       2610       2620       2630       2640 
RVLLQLKYRY DVEIDNSSRS ALKKILERDD TAAKTLVLCV SDIISLSTNV SETSGSKASS 

      2650       2660       2670       2680       2690       2700 
EDSNKVDTIE LTDGWYAVKA QLDPPLLALV KSGRLTVGQK IITQGAELVG SPDACAPLEA 

      2710       2720       2730       2740       2750       2760 
PDSLRLKISA NSTRPARWHS KLGFFHDPRP FPLPLSSLFS DGGNVGCVDV IVQRVYPLQW 

      2770       2780       2790       2800       2810       2820 
VEKTVSGSYI FRNEREEEKE ALRFAEAQQK KLEALFTKVH TELKEHEEDI AQRRVLSRAL 

      2830       2840       2850       2860       2870       2880 
TRQQVHALQD GAELYAAVQD ASDPEHLETC FSEEQLRALN NYRQMLSDKK QARIQSEFRK 

      2890       2900       2910       2920       2930       2940 
ALEAAEKEEG LSRDVSTVWK LRVTSYKKRE KSALLSIWRP SSDLPSLLTE GQRYRIYHLS 

      2950       2960       2970       2980       2990       3000 
VSKSKNKFEW PSIQLTATKR TQYQQLPVSS ETLLQLYQPR ELLPFSKLSD PAFQPPCSEV 

      3010       3020       3030       3040       3050       3060 
DVVGVVVSVV KPIGLAPLVY LSDECLHLLV VKFGIDLNED IKPRVLIAAS NLQWRPESTS 

      3070       3080       3090       3100       3110       3120 
RVPTLFAGNF SVFSASPKEA HFQERVTNMK HAIENIDTFY KEAEKKLIQV LKGDSPKWST 

      3130       3140       3150       3160       3170       3180 
PNKDPTREPY PASTCSASDL ASGGQLPRSS PTDQQSYRSP LSCCTPTGKS TPLAHSAWMA 

      3190       3200       3210       3220       3230       3240 
AKSCSGENEI EDPKTCRKKR ALDLLSRLPL PPPLSPVCTF VSPAAQKAFQ PPRSCGTKYP 

      3250       3260       3270       3280       3290       3300 
TPLKKEGPSS PWSRAPFQKA SGVSLLDCDS VADEELALLS TQALVPHSVG GSEQVFPSDS 

      3310       3320       3330       3340 
TRTEGPSAST EARPANRSKR ESLRDCRDDS DGKLAAETVP DYS 

« Hide

References

[1]"Characterization of the rat and mouse homologues of the BRCA2 breast cancer susceptibility gene."
McAllister K.A., Haugen-Strano A., Hagevik S., Brownlee H.A., Collins N.K., Futreal P.A., Bennett L.M., Wiseman R.W.
Cancer Res. 57:3121-3125(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Testis.
[2]"BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure."
Yang H., Jeffrey P.D., Miller J., Kinnucan E., Sun Y., Thoma N.H., Zheng N., Chen P.L., Lee W.H., Pavletich N.P.
Science 297:1837-1848(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 2335-3151 IN COMPLEX WITH DSS1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U89653 mRNA. Translation: AAB71378.1.
PIRT42207.
UniGeneRn.103225.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IYJX-ray3.40B/D2335-3151[»]
ProteinModelPortalO35923.
SMRO35923. Positions 1505-1536, 2403-3112.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbO35923.
PRIDEO35923.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2219. rat.

Organism-specific databases

RGD2219. Brca2.

Phylogenomic databases

eggNOGNOG331296.
HOGENOMHOG000139693.
HOVERGENHBG050731.
InParanoidO35923.
PhylomeDBO35923.

Gene expression databases

GenevestigatorO35923.

Family and domain databases

Gene3D2.40.50.140. 4 hits.
InterProIPR015525. BRCA2.
IPR015187. BRCA2_OB_1.
IPR015188. BRCA2_OB_3.
IPR002093. BRCA2_repeat.
IPR015252. DNA_recomb/repair_BRCA2_hlx.
IPR012340. NA-bd_OB-fold.
IPR015205. Tower.
[Graphical view]
PANTHERPTHR11289. PTHR11289. 1 hit.
PfamPF09169. BRCA-2_helical. 1 hit.
PF09103. BRCA-2_OB1. 1 hit.
PF09104. BRCA-2_OB3. 1 hit.
PF00634. BRCA2. 7 hits.
PF09121. Tower. 1 hit.
[Graphical view]
PIRSFPIRSF002397. BRCA2. 1 hit.
SUPFAMSSF50249. SSF50249. 4 hits.
SSF81872. SSF81872. 1 hit.
PROSITEPS50138. BRCA2_REPEAT. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO35923.
NextBio21679528.
PROO35923.

Entry information

Entry nameBRCA2_RAT
AccessionPrimary (citable) accession number: O35923
Entry history
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: January 1, 1998
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references