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Protein

Calpain-9

Gene

Capn9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.By similarity

Catalytic activityi

Broad endopeptidase specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi81 – 811Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi83 – 831Calcium 1; via carbonyl oxygenBy similarity
Metal bindingi88 – 881Calcium 1By similarity
Active sitei97 – 971By similarity
Metal bindingi167 – 1671Calcium 1By similarity
Active sitei254 – 2541By similarity
Active sitei278 – 2781By similarity
Metal bindingi284 – 2841Calcium 2By similarity
Metal bindingi291 – 2911Calcium 2By similarity
Metal bindingi312 – 3121Calcium 2; via carbonyl oxygen
Metal bindingi314 – 3141Calcium 2By similarity
Metal bindingi316 – 3161Calcium 2; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi574 – 585121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi604 – 615122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: RGD
  • calcium ion binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B29. 5301.

Protein family/group databases

MEROPSiC02.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-9 (EC:3.4.22.-)
Alternative name(s):
Digestive tract-specific calpain
New calpain 4
Short name:
nCL-4
Gene namesi
Name:Capn9
Synonyms:Ncl4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi70965. Capn9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Calpain-9PRO_0000207724Add
BLAST

Proteomic databases

PaxDbiO35920.
PRIDEiO35920.

Expressioni

Tissue specificityi

Predominantly expressed in stomach and small intestine, although low levels of expression in other organs.

Gene expression databases

ExpressionAtlasiO35920. baseline and differential.
GenevisibleiO35920. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025357.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 337296Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini518 – 55235EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini561 – 59434EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini591 – 62636EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni338 – 521184Domain IIIAdd
BLAST
Regioni522 – 690169Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO35920.
KOiK08578.
OrthoDBiEOG7RV9FM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029542. CAPN9.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF311. PTHR10183:SF311. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYLHRSLRP QPQPVPRDAR TVHSSGHSFE QLRQSCLQSG TLFEDADFPA
60 70 80 90 100
SNSSLFYSER PQVPFVWKRP GEIVENPEFI LGGATRTDIC QGELGDCWLL
110 120 130 140 150
AAIASLTLNQ KALARVVPQD QGFGSGYAGI FHFQFWQHSE WLDVVIDDRL
160 170 180 190 200
PTFRDRLVFL HSADHNEFWS ALLEKAYAKL NGSYEALKGG SAIEAMEDFT
210 220 230 240 250
GGVAENFQIR EAPEDFYEIL EKALRRGSLL GCSIDTLNAS ESEARTPLGL
260 270 280 290 300
IKGHAYTVTG LDQVNFHGQR IKLIRVRNPW GQVEWNGPWS DSSPEWRSMS
310 320 330 340 350
LEEQKRLGHT ALDDGEFWMA FEDFKTHFDK VEICNLTPDA LEDNTLHKWE
360 370 380 390 400
VTIHQGSWVR GSTAGGCRNF LDTFWTNPQI KLSLTERDEG QEGCTFLAAL
410 420 430 440 450
MQKDRRRLKR FGANMLTIGY AIYQCPDKDG HLNRDFFRYH ASLARSKTFI
460 470 480 490 500
NLREVSGRFQ LPPGDYILIP STFEPHQEAD FCLRIFSEKK AVTQDLDENI
510 520 530 540 550
DIDLPELPKP TPQEEETEEE RQFRALFRRI AGEDMEVSAE ELEYVLNAVL
560 570 580 590 600
QKKTALKFKR LSLLSCRNII SLMDTSGNGK MEFEEFRVFW DKLRYWMDLF
610 620 630 640 650
LQFDVDKSGT MSSYELRTAL KAAGFQLGSH LLQLIVLRYA DENLQLDFDD
660 670 680 690
YLNCLVRLEN ASRVFQCLSV KNKDFIHLNI NEFINLTMNI
Length:690
Mass (Da):79,263
Last modified:February 6, 2013 - v2
Checksum:i4370589D108800F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti299 – 2991M → V in AAB69115 (PubMed:9524069).Curated
Sequence conflicti559 – 5591K → KRL in AAB69115 (PubMed:9524069).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89514 mRNA. Translation: AAB69115.1.
RefSeqiNP_001258069.1. NM_001271140.1.
UniGeneiRn.10804.

Genome annotation databases

EnsembliENSRNOT00000025357; ENSRNOP00000025357; ENSRNOG00000018480.
GeneIDi116694.
KEGGirno:116694.
UCSCiRGD:70965. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89514 mRNA. Translation: AAB69115.1.
RefSeqiNP_001258069.1. NM_001271140.1.
UniGeneiRn.10804.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025357.

Protein family/group databases

MEROPSiC02.006.

Proteomic databases

PaxDbiO35920.
PRIDEiO35920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025357; ENSRNOP00000025357; ENSRNOG00000018480.
GeneIDi116694.
KEGGirno:116694.
UCSCiRGD:70965. rat.

Organism-specific databases

CTDi10753.
RGDi70965. Capn9.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiO35920.
KOiK08578.
OrthoDBiEOG7RV9FM.

Enzyme and pathway databases

BRENDAi3.4.22.B29. 5301.

Miscellaneous databases

NextBioi619572.
PROiO35920.

Gene expression databases

ExpressionAtlasiO35920. baseline and differential.
GenevisibleiO35920. RN.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029542. CAPN9.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF311. PTHR10183:SF311. 1 hit.
PfamiPF01067. Calpain_III. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "Molecular cloning and characterization of a novel tissue-specific calpain predominantly expressed in the digestive tract."
    Lee H.-J., Sorimachi H., Jeong S.-Y., Ishiura S., Suzuki K.
    Biol. Chem. 379:175-183(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 19-690.

Entry informationi

Entry nameiCAN9_RAT
AccessioniPrimary (citable) accession number: O35920
Secondary accession number(s): F1LR50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: February 6, 2013
Last modified: February 17, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.