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Protein

Zinc finger protein basonuclin-1

Gene

Bnc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator (PubMed:9687312, PubMed:23707421). Likely specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation (By similarity). Required for the maintenance of spermatogenesis (PubMed:22266914). May also play a role in the differentiation of oocytes and the early development of embryos (PubMed:9687312).By similarity3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri325 – 348C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri353 – 382C2H2-type 2PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri687 – 711C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri715 – 743C2H2-type 4PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri895 – 918C2H2-type 5PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri923 – 950C2H2-type 6PROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • chromosome organization Source: MGI
  • positive regulation of epithelial cell proliferation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription from RNA polymerase I promoter Source: MGI
  • spermatogenesis Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
  • wound healing Source: MGI

Keywordsi

Molecular functionDNA-binding
Biological processDifferentiation, Spermatogenesis, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein basonuclin-1
Gene namesi
Name:Bnc1
Synonyms:Bnc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1097164. Bnc1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Viable, although fertility is severely reduced in both males and females. Males have significantly reduced testis size, with progressive degeneration of seminiferous epithelium and loss of germ cells from 8 weeks of age. Sperm motility is also reduced.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000469331 – 961Zinc finger protein basonuclin-1Add BLAST961

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei505PhosphoserineBy similarity1
Modified residuei509PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation on Ser-505 and Ser-509 leads to cytoplasmic localization.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35914.
PRIDEiO35914.

PTM databases

iPTMnetiO35914.
PhosphoSitePlusiO35914.

Expressioni

Tissue specificityi

Epidermis and germ cells of testis and ovary.1 Publication

Developmental stagei

Appears to be expressed by primary and secondary spermatocytes and spermatids. Detected in oocytes within the primary follicles. It is also present in secondary oocytes.1 Publication

Gene expression databases

CleanExiMM_BNC1.

Interactioni

Subunit structurei

Interacts with HSF2BP (via C-terminus).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Hsf2bpQ9D4G24EBI-8527667,EBI-8527688

Protein-protein interaction databases

IntActiO35914. 1 interactor.
STRINGi10090.ENSMUSP00000026096.

Structurei

3D structure databases

ProteinModelPortaliO35914.
SMRiO35914.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni210 – 219Hydrophobic10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi501 – 507Nuclear localization signalBy similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi826 – 843Ser-richAdd BLAST18

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri325 – 348C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri353 – 382C2H2-type 2PROSITE-ProRule annotationAdd BLAST30
Zinc fingeri687 – 711C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri715 – 743C2H2-type 4PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri895 – 918C2H2-type 5PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri923 – 950C2H2-type 6PROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IE3V. Eukaryota.
ENOG410YN2D. LUCA.
HOGENOMiHOG000049098.
HOVERGENiHBG050706.
InParanoidiO35914.
PhylomeDBiO35914.

Family and domain databases

InterProiView protein in InterPro
IPR036236. Znf_C2H2_sf.
IPR013087. Znf_C2H2_type.
SMARTiView protein in SMART
SM00355. ZnF_C2H2. 6 hits.
SUPFAMiSSF57667. SSF57667. 1 hit.
PROSITEiView protein in PROSITE
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.

Sequencei

Sequence statusi: Complete.

O35914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAIGCTLN CSCQCFKPGK INHRQCEQCR HGWVAHALSK LRIPPVYPTS
60 70 80 90 100
QVEIVQSNVV FDISSLMLYG TQAIPVRLKI LLDRLFSVLK QDEVLQILHA
110 120 130 140 150
LDWTLQDYIR GYVLQDASGK VLDHWSIMTS EEEVATLQQF LRFGETKSIV
160 170 180 190 200
ELMAIQEKEE QSVIVPPTTA NVDIRAFIES CGHRSASLPT PVDKGSPGGM
210 220 230 240 250
HPFENLISNM TFMLPFQFFN PLPPALIGSL PEQYMLEQGQ DQSQEPKQEL
260 270 280 290 300
HGPFSDSSFL TSTPFQVEKE QCLNCPETVP QKEDSAHLSD SSSYSIASKL
310 320 330 340 350
ERTQLSPEAK VKPERNSLSA KKGRVFCTAC EKTFYDKGTL KIHYNAVHLK
360 370 380 390 400
IKHKCTIEGC NMVFSSLRSR NRHSANPNPR LHMPMNRNNR DKDLRNSLNL
410 420 430 440 450
ASSETYKRPG FTVVSPDCGP LPGYTGSVED SKGQPAFSSI GQNGVLFPNL
460 470 480 490 500
KTVQPVLPFY RSPATPAELA NTPGMLPSLP LLSSSIPEQL VSTDMPFDAL
510 520 530 540 550
PKKKSRKSSM PIKIEKEAVE IAEEKRHSLS SDDEVPLQVV SEDEPEDSSP
560 570 580 590 600
RSDRVPEEQH TQLSLEEPLP QGERACHLES VIESHGALSR TLEQTTLTER
610 620 630 640 650
EAEQKVALSS VMPREVEDGG HERHFTAGLV PQIPFPDYME LQQRLLAGGL
660 670 680 690 700
FGALSNRGMA FPFLEESKEL EHLGEHALVR QKEEARFQCD ICKKTFKNAC
710 720 730 740 750
SMKTHEKNTH ARETHACTVE GCGAAFPSRR SRDRHSSNLS LHQKVLNEEA
760 770 780 790 800
LETSEDHFRA AYLLQDVAKE AYQDVAFTPQ ASQTSVIFKG TSGMGSLVYP
810 820 830 840 850
ISQVHSASLE SYNSGPPSEG TILDLSTTSS MKSESSSHSS WDSDGVSEEG
860 870 880 890 900
TALMEDSDGN CEGQSLVSGE DEYPLCVLME KADQSLASLP SGLPITCHLC
910 920 930 940 950
QKIYSNKGTF RAHYKTVHLR QLHKCKVPGC NTMFSSVRSR NRHSQNPNLH
960
KSLASSPSHL Q
Length:961
Mass (Da):106,659
Last modified:January 1, 1998 - v1
Checksum:i82CF949BF913C68F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti100A → V in AAB94675 (PubMed:9687312).Curated1
Sequence conflicti234Y → D in AAB94675 (PubMed:9687312).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88064 mRNA. Translation: AAC53347.1.
AF025301 mRNA. Translation: AAB94675.1.
UniGeneiMm.243802.

Similar proteinsi

Entry informationi

Entry nameiBNC1_MOUSE
AccessioniPrimary (citable) accession number: O35914
Secondary accession number(s): O54886
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: October 25, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot