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Protein

Solute carrier organic anion transporter family member 1A4

Gene

Slco1a4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates the Na+-independent transport of organic anions such as taurocholate, cholate, 17-beta-glucuronosyl estradiol, estrone-3-sulfate, the cardiac glycosides ouabain and digoxin and thyroid hormones. May play an especially important role in the brain accumulation and toxicity of digoxin and in the hepatobiliary and renal excretion of cardiac glycosides.

GO - Molecular functioni

  • bile acid transmembrane transporter activity Source: RGD
  • organic anion transmembrane transporter activity Source: RGD

GO - Biological processi

  • bile acid and bile salt transport Source: GOC
  • sodium-independent organic anion transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-159418. Recycling of bile acids and salts.
R-RNO-879518. Transport of organic anions.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier organic anion transporter family member 1A4
Alternative name(s):
Brain digoxin carrier protein
Brain-specific organic anion transporter
OATP-B1
Sodium-independent organic anion-transporting polypeptide 2
Solute carrier family 21 member 5
Gene namesi
Name:Slco1a4
Synonyms:Oatp1a4, Oatp2, Slc21a5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi621389. Slco1a4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence analysisAdd
BLAST
Transmembranei21 – 4020Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini41 – 5919ExtracellularSequence analysisAdd
BLAST
Transmembranei60 – 8021Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini81 – 866CytoplasmicSequence analysis
Transmembranei87 – 11125Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini112 – 15443ExtracellularSequence analysisAdd
BLAST
Transmembranei155 – 18329Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini184 – 20219CytoplasmicSequence analysisAdd
BLAST
Transmembranei203 – 22321Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini224 – 24118ExtracellularSequence analysisAdd
BLAST
Transmembranei242 – 26625Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini267 – 31044CytoplasmicSequence analysisAdd
BLAST
Transmembranei311 – 33222Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini333 – 35220ExtracellularSequence analysisAdd
BLAST
Transmembranei353 – 37624Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini377 – 3804CytoplasmicSequence analysis
Transmembranei381 – 40424Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini405 – 512108ExtracellularSequence analysisAdd
BLAST
Transmembranei513 – 53523Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini536 – 5449CytoplasmicSequence analysis
Transmembranei545 – 57026Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini571 – 60434ExtracellularSequence analysisAdd
BLAST
Transmembranei605 – 62218Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini623 – 66139CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1781860.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661Solute carrier organic anion transporter family member 1A4PRO_0000191047Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi438 ↔ 464PROSITE-ProRule annotation
Disulfide bondi442 ↔ 453PROSITE-ProRule annotation
Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence analysis
Disulfide bondi444 ↔ 468PROSITE-ProRule annotation
Glycosylationi482 – 4821N-linked (GlcNAc...)Sequence analysis
Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence analysis
Modified residuei633 – 6331PhosphoserineBy similarity
Modified residuei634 – 6341PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO35913.
PRIDEiO35913.

Expressioni

Tissue specificityi

Highly expressed in brain, liver, and kidney but not expressed in heart, spleen, lung, skeletal muscle, and testis.

Gene expression databases

GenevisibleiO35913. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040237.

Chemistry

BindingDBiO35913.

Structurei

3D structure databases

ProteinModelPortaliO35913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini432 – 48756Kazal-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3626. Eukaryota.
ENOG410XRSF. LUCA.
GeneTreeiENSGT00760000119014.
HOVERGENiHBG063896.
InParanoidiO35913.
KOiK03460.
OMAiCHAGCKE.
OrthoDBiEOG7GFB4C.
PhylomeDBiO35913.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR020846. MFS_dom.
IPR004156. OA_transporter.
[Graphical view]
PANTHERiPTHR11388. PTHR11388. 1 hit.
PfamiPF07648. Kazal_2. 1 hit.
PF03137. OATP. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00805. oat. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35913-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSEKRVAT HGVRCFAKIK MFLLALTCAY VSKSLSGTYM NSMLTQIERQ
60 70 80 90 100
FGIPTSIVGL INGSFEIGNL LLIIFVSYFG TKLHRPIMIG VGCAVMGLGC
110 120 130 140 150
FLISLPHFLM GQYEYETILP TSNVSSNSFF CVENRSQTLN PTQDPSECVK
160 170 180 190 200
EMKSLMWIYV LVGNIIRGIG ETPIMPLGIS YIEDFAKSEN SPLYIGILET
210 220 230 240 250
GMTIGPLIGL LLASSCANIY VDIESVNTDD LTITPTDTRW VGAWWIGFLV
260 270 280 290 300
CAGVNILTSF PFFFFPKTLP KEGLQENVDG TENAKEKKHR KKAKEEKRGI
310 320 330 340 350
TKDFFVFMKS LSCNPIYMLF ILISVLQFNA FINSFTFMPK YLEQQYGKST
360 370 380 390 400
AEVVFLMGLY MLPPICLGYL IGGLIMKKFK VTVKKAAHLA FWLCLSEYLL
410 420 430 440 450
SFLSYVMTCD NFPVAGLTTS YEGVQHQLYV ENKVLADCNT RCNCSTNTWD
460 470 480 490 500
PVCGDNGLAY MSACLAGCEK SVGTGTNMVF QNCSCIQSSG NSSAVLGLCN
510 520 530 540 550
KGPDCANKLQ YFLIIAIFGC FIYSLAGIPG YMVLLRCIKS EEKSLGVGLH
560 570 580 590 600
AFCIRILAGI PAPIYFGALI DRTCLHWGTL KCGEPGACRM YDINSFRRLY
610 620 630 640 650
LGLPAALRGA SFVPAFFILR LTRTFQFPGD IESSKTDHAE MKLTLKESEC
660
TEVLRSKVTE D
Length:661
Mass (Da):73,252
Last modified:January 1, 1998 - v1
Checksum:i692DA69FC85299D2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti360 – 3601Y → N in AAC32669 (PubMed:9712861).Curated
Sequence conflicti617 – 6171F → FF in AAC32669 (PubMed:9712861).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88036 mRNA. Translation: AAB80699.1.
U95011 mRNA. Translation: AAC32669.1.
RefSeqiNP_571981.1. NM_131906.1.
XP_008761573.1. XM_008763351.1.
UniGeneiRn.5641.

Genome annotation databases

EnsembliENSRNOT00000043374; ENSRNOP00000042595; ENSRNOG00000047493.
ENSRNOT00000046598; ENSRNOP00000040237; ENSRNOG00000047493.
GeneIDi170698.
KEGGirno:170698.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88036 mRNA. Translation: AAB80699.1.
U95011 mRNA. Translation: AAC32669.1.
RefSeqiNP_571981.1. NM_131906.1.
XP_008761573.1. XM_008763351.1.
UniGeneiRn.5641.

3D structure databases

ProteinModelPortaliO35913.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000040237.

Chemistry

BindingDBiO35913.
ChEMBLiCHEMBL1781860.

Proteomic databases

PaxDbiO35913.
PRIDEiO35913.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000043374; ENSRNOP00000042595; ENSRNOG00000047493.
ENSRNOT00000046598; ENSRNOP00000040237; ENSRNOG00000047493.
GeneIDi170698.
KEGGirno:170698.

Organism-specific databases

CTDi6579.
RGDi621389. Slco1a4.

Phylogenomic databases

eggNOGiKOG3626. Eukaryota.
ENOG410XRSF. LUCA.
GeneTreeiENSGT00760000119014.
HOVERGENiHBG063896.
InParanoidiO35913.
KOiK03460.
OMAiCHAGCKE.
OrthoDBiEOG7GFB4C.
PhylomeDBiO35913.

Enzyme and pathway databases

ReactomeiR-RNO-159418. Recycling of bile acids and salts.
R-RNO-879518. Transport of organic anions.

Miscellaneous databases

NextBioi621183.
PROiO35913.

Gene expression databases

GenevisibleiO35913. RN.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR020846. MFS_dom.
IPR004156. OA_transporter.
[Graphical view]
PANTHERiPTHR11388. PTHR11388. 1 hit.
PfamiPF07648. Kazal_2. 1 hit.
PF03137. OATP. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00805. oat. 1 hit.
PROSITEiPS51465. KAZAL_2. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a multispecific organic anion and cardiac glycoside transporter from rat brain."
    Noe B., Hagenbuch B., Stieger B., Meier P.J.
    Proc. Natl. Acad. Sci. U.S.A. 94:10346-10350(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholate and comparison with oatp2."
    Abe T., Kakyo M., Sakagami H., Tokui T., Nishio T., Tanemoto M., Nomura H., Hebert S.C., Matsuno S., Kondo H., Yawo H.
    J. Biol. Chem. 273:22395-22401(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.

Entry informationi

Entry nameiSO1A4_RAT
AccessioniPrimary (citable) accession number: O35913
Secondary accession number(s): O55224
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.