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O35906 (SPIB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription factor Spi-B
Gene names
Name:Spib
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Subunit structure

Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in the medulla of the thymus, the spleen and germinal centers of the lymph nodes. Expressed in B-cells and T-cells, expression increases during B-cell maturation and decreases during T-cell maturation. Ref.2

Domain

The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes By similarity.

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]
Isoform IA (identifier: O35906-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.
Isoform IC (identifier: O35906-2)

The sequence of this isoform differs from the canonical sequence as follows:
     17-17: L → LVGEGVSPRPEMTLSSLRQ
Note: Produced by alternative splicing of isoform IA. No experimental confirmation available.
Isoform IIA (identifier: O35906-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MLALEAAQ → MPLCLSPAR
Note: Produced by alternative promoter usage.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267Transcription factor Spi-B
PRO_0000204137

Regions

DNA binding174 – 25784ETS
Region1 – 3131TAD1 (Acidic)
Region41 – 6222TAD2

Natural variations

Alternative sequence1 – 88MLALEAAQ → MPLCLSPAR in isoform IIA.
VSP_013907
Alternative sequence171L → LVGEGVSPRPEMTLSSLRQ in isoform IC.
VSP_013908

Sequences

Sequence LengthMass (Da)Tools
Isoform IA [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: D32485A93C44327F

FASTA26729,365
        10         20         30         40         50         60 
MLALEAAQLD GPHLSCLYPE GVFYDLDSCK PFSYPDSDGG LDSTWGWTEA PPAPAIAPYE 

        70         80         90        100        110        120 
AFDPATAAFS HSQTVQLCYS HGPNPSTYSP MGTLDPAPSL EAPGPGLQVY PPEDFTSQTL 

       130        140        150        160        170        180 
GSLAYAPYPS PVLSEEEDIM LDSPALEVSD SESDEALLAG SEGRGSEAGA RKKLRLYQFL 

       190        200        210        220        230        240 
LGLLLRGDMR ECVWWVEPGA GVFQFSSKHK ELLARRWGQQ KGNRKRMTYQ KLARALRNYA 

       250        260 
KTGEIRKVKR KLTYQFDSAL LPASRHV 

« Hide

Isoform IC [UniParc].

Checksum: AA7AC02B7E1AACC9
Show »

FASTA28531,290
Isoform IIA [UniParc].

Checksum: 0FBAD29580730610
Show »

FASTA26829,506

References

[1]"Two promoters direct expression of the murine Spi-B gene, an Ets family transcription factor."
Chen H.-M., Gonzalez D.A., Radomska H.S., Voso M.T., Sun Z., Zhang P., Zhang D.-E., Tenen D.G.
Gene 207:209-218(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS IA; IC AND IIA), ALTERNATIVE PROMOTER USAGE.
Strain: 129/Sv.
[2]"The Ets protein Spi-B is expressed exclusively in B cells and T cells during development."
Su G.H., Ip H.S., Cobb B.S., Lu M.-M., Chen H.-M., Simon M.C.
J. Exp. Med. 184:203-214(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
[3]"Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B."
Su G.H., Chen H.-M., Muthusamy N., Garrett-Sinha L.A., Baunoch D., Tenen D.G., Simon M.C.
EMBO J. 16:7118-7129(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction."
Garrett-Sinha L.A., Su G.H., Rao S., Kabak S., Hao Z., Clark M.R., Simon M.C.
Immunity 10:399-408(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1."
Garrett-Sinha L.A., Dahl R., Rao S., Barton K.P., Simon M.C.
Blood 97:2908-2912(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"PU.1/Spi-B regulation of c-rel is essential for mature B cell survival."
Hu C.-J., Rao S., Ramirez-Bergeron D.L., Garrett-Sinha L.A., Gerondakis S., Clark M.R., Simon M.C.
Immunity 15:545-555(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Spi-B can functionally replace PU.1 in myeloid but not lymphoid development."
Dahl R., Ramirez-Bergeron D.L., Rao S., Simon M.C.
EMBO J. 21:2220-2230(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The transcription factor Spi-B is not required for somatic hypermutation."
Kim N., Martin T.E., Simon M.C., Storb U.
Mol. Immunol. 39:577-583(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U87619 expand/collapse EMBL AC list , U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53554.1.
U87619 expand/collapse EMBL AC list , U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53556.1.
U87619 expand/collapse EMBL AC list , U87616, U87617, U87618 Genomic DNA. Translation: AAC53557.1.
U87620 Genomic DNA. Translation: AAC53559.1.
RefSeqNP_063919.1. NM_019866.1.
XP_006540996.1. XM_006540933.1.
XP_006540998.1. XM_006540935.1.
UniGeneMm.8012.

3D structure databases

ProteinModelPortalO35906.
SMRO35906. Positions 173-256.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbO35906.
PRIDEO35906.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000035323; ENSMUSP00000035539; ENSMUSG00000008193. [O35906-1]
ENSMUST00000098483; ENSMUSP00000096084; ENSMUSG00000008193. [O35906-2]
GeneID272382.
KEGGmmu:272382.
UCSCuc009gpw.1. mouse. [O35906-1]

Organism-specific databases

CTD6689.
MGIMGI:892986. Spib.

Phylogenomic databases

eggNOGNOG241814.
GeneTreeENSGT00390000015212.
HOGENOMHOG000095520.
HOVERGENHBG002474.
KOK09439.
OMAATFSHPQ.
OrthoDBEOG78M02J.
PhylomeDBO35906.
TreeFamTF352494.

Gene expression databases

BgeeO35906.
CleanExMM_SPIB.
GenevestigatorO35906.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00178. Ets. 1 hit.
[Graphical view]
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
[Graphical view]
PROSITEPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio393578.
PROO35906.
SOURCESearch...

Entry information

Entry nameSPIB_MOUSE
AccessionPrimary (citable) accession number: O35906
Secondary accession number(s): O35907, O35909, O55199
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot