Transcription factor Spi-B - Mus musculus (Mouse)

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O35906 (SPIB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transcription factor Spi-B

Gene names

Name:

Spib

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Ref.2 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Subunit structure	Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in the medulla of the thymus, the spleen and germinal centers of the lymph nodes. Expressed in B-cells and T-cells, expression increases during B-cell maturation and decreases during T-cell maturation. Ref.2
Domain	The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes By similarity.
Sequence similarities	Belongs to the ETS family. Contains 1 ETS DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative promoter usage Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	macrophage differentiation Inferred from direct assay Ref.7. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor complex Inferred by curator Ref.7. Source: MGI
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay Ref.7. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. [Align] [Select]

Isoform IA (identifier: O35906-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by alternative promoter usage.

Isoform IC (identifier: O35906-2) The sequence of this isoform differs from the canonical sequence as follows: 17-17: L → LVGEGVSPRPEMTLSSLRQ
Note: Produced by alternative splicing of isoform IA. No experimental confirmation available.

Isoform IIA (identifier: O35906-3) The sequence of this isoform differs from the canonical sequence as follows: 1-8: MLALEAAQ → MPLCLSPAR
Note: Produced by alternative promoter usage.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 267

267

Transcription factor Spi-B

PRO_0000204137

Regions

DNA binding

174 – 257

ETS

Region

1 – 31

TAD1 (Acidic)

Region

41 – 62

TAD2

Natural variations

Alternative sequence

1 – 8

MLALEAAQ → MPLCLSPAR in isoform IIA.

VSP_013907

Alternative sequence

L → LVGEGVSPRPEMTLSSLRQ in isoform IC.

VSP_013908

Sequences

Sequence		Length	Mass (Da)
Isoform IA [UniParc]. Last modified June 21, 2005. Version 2. Checksum: D32485A93C44327F Show »	FASTA	267	29,365
10 20 30 40 50 60 MLALEAAQLD GPHLSCLYPE GVFYDLDSCK PFSYPDSDGG LDSTWGWTEA PPAPAIAPYE 70 80 90 100 110 120 AFDPATAAFS HSQTVQLCYS HGPNPSTYSP MGTLDPAPSL EAPGPGLQVY PPEDFTSQTL 130 140 150 160 170 180 GSLAYAPYPS PVLSEEEDIM LDSPALEVSD SESDEALLAG SEGRGSEAGA RKKLRLYQFL 190 200 210 220 230 240 LGLLLRGDMR ECVWWVEPGA GVFQFSSKHK ELLARRWGQQ KGNRKRMTYQ KLARALRNYA 250 260 KTGEIRKVKR KLTYQFDSAL LPASRHV « Hide
Isoform IC [UniParc]. Checksum: AA7AC02B7E1AACC9 Show »	FASTA	285	31,290
10 20 30 40 50 60 MLALEAAQLD GPHLSCLVGE GVSPRPEMTL SSLRQYPEGV FYDLDSCKPF SYPDSDGGLD 70 80 90 100 110 120 STWGWTEAPP APAIAPYEAF DPATAAFSHS QTVQLCYSHG PNPSTYSPMG TLDPAPSLEA 130 140 150 160 170 180 PGPGLQVYPP EDFTSQTLGS LAYAPYPSPV LSEEEDIMLD SPALEVSDSE SDEALLAGSE 190 200 210 220 230 240 GRGSEAGARK KLRLYQFLLG LLLRGDMREC VWWVEPGAGV FQFSSKHKEL LARRWGQQKG 250 260 270 280 NRKRMTYQKL ARALRNYAKT GEIRKVKRKL TYQFDSALLP ASRHV « Hide
Isoform IIA [UniParc]. Checksum: 0FBAD29580730610 Show »	FASTA	268	29,506
10 20 30 40 50 60 MPLCLSPARL DGPHLSCLYP EGVFYDLDSC KPFSYPDSDG GLDSTWGWTE APPAPAIAPY 70 80 90 100 110 120 EAFDPATAAF SHSQTVQLCY SHGPNPSTYS PMGTLDPAPS LEAPGPGLQV YPPEDFTSQT 130 140 150 160 170 180 LGSLAYAPYP SPVLSEEEDI MLDSPALEVS DSESDEALLA GSEGRGSEAG ARKKLRLYQF 190 200 210 220 230 240 LLGLLLRGDM RECVWWVEPG AGVFQFSSKH KELLARRWGQ QKGNRKRMTY QKLARALRNY 250 260 AKTGEIRKVK RKLTYQFDSA LLPASRHV « Hide

References

[1]	"Two promoters direct expression of the murine Spi-B gene, an Ets family transcription factor." Chen H.-M., Gonzalez D.A., Radomska H.S., Voso M.T., Sun Z., Zhang P., Zhang D.-E., Tenen D.G. Gene 207:209-218(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS IA; IC AND IIA), ALTERNATIVE PROMOTER USAGE. Strain: 129/Sv.
[2]	"The Ets protein Spi-B is expressed exclusively in B cells and T cells during development." Su G.H., Ip H.S., Cobb B.S., Lu M.-M., Chen H.-M., Simon M.C. J. Exp. Med. 184:203-214(1996) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[3]	"Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B." Su G.H., Chen H.-M., Muthusamy N., Garrett-Sinha L.A., Baunoch D., Tenen D.G., Simon M.C. EMBO J. 16:7118-7129(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction." Garrett-Sinha L.A., Su G.H., Rao S., Kabak S., Hao Z., Clark M.R., Simon M.C. Immunity 10:399-408(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1." Garrett-Sinha L.A., Dahl R., Rao S., Barton K.P., Simon M.C. Blood 97:2908-2912(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"PU.1/Spi-B regulation of c-rel is essential for mature B cell survival." Hu C.-J., Rao S., Ramirez-Bergeron D.L., Garrett-Sinha L.A., Gerondakis S., Clark M.R., Simon M.C. Immunity 15:545-555(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Spi-B can functionally replace PU.1 in myeloid but not lymphoid development." Dahl R., Ramirez-Bergeron D.L., Rao S., Simon M.C. EMBO J. 21:2220-2230(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"The transcription factor Spi-B is not required for somatic hypermutation." Kim N., Martin T.E., Simon M.C., Storb U. Mol. Immunol. 39:577-583(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U87619 , U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53554.1. U87619 , U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53556.1. U87619 , U87616, U87617, U87618 Genomic DNA. Translation: AAC53557.1. U87620 Genomic DNA. Translation: AAC53559.1.
IPI	IPI00553443. IPI00607930. IPI00608035.
RefSeq	NP_063919.1. NM_019866.1.
UniGene	Mm.8012.
3D structure databases
ProteinModelPortal	O35906.
SMR	O35906. Positions 173-256.
ModBase	Search...
Proteomic databases
PaxDb	O35906.
PRIDE	O35906.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035323; ENSMUSP00000035539; ENSMUSG00000008193. ENSMUST00000098483; ENSMUSP00000096084; ENSMUSG00000008193.
GeneID	272382.
KEGG	mmu:272382.
UCSC	uc009gpw.1. mouse.
Organism-specific databases
CTD	6689.
MGI	MGI:892986. Spib.
Phylogenomic databases
eggNOG	NOG241814.
GeneTree	ENSGT00390000015212.
HOGENOM	HOG000095520.
HOVERGEN	HBG002474.
KO	K09439.
OMA	QLDGPHF.
OrthoDB	EOG4FXR8B.
Gene expression databases
Bgee	O35906.
CleanEx	MM_SPIB.
Genevestigator	O35906.
GermOnline	ENSMUSG00000008193. Mus musculus.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000418. Ets_dom. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00178. Ets. 1 hit. [Graphical view]
PRINTS	PR00454. ETSDOMAIN.
SMART	SM00413. ETS. 1 hit. [Graphical view]
PROSITE	PS00345. ETS_DOMAIN_1. 1 hit. PS00346. ETS_DOMAIN_2. 1 hit. PS50061. ETS_DOMAIN_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	393578.
SOURCE	Search...

Entry information

Entry name

SPIB_MOUSE

Accession

Primary (citable) accession number: O35906
Secondary accession number(s): O35907, O35909, O55199

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents