Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O35906

- SPIB_MOUSE

UniProt

O35906 - SPIB_MOUSE

Protein

Transcription factor Spi-B

Gene

Spib

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.7 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi174 – 25784ETSPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
    3. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. macrophage differentiation Source: MGI
    2. regulation of transcription, DNA-templated Source: MGI
    3. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    4. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription factor Spi-B
    Gene namesi
    Name:Spib
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:892986. Spib.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: RefGenome
    2. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 267267Transcription factor Spi-BPRO_0000204137Add
    BLAST

    Proteomic databases

    MaxQBiO35906.
    PaxDbiO35906.
    PRIDEiO35906.

    Expressioni

    Tissue specificityi

    Expressed in the medulla of the thymus, the spleen and germinal centers of the lymph nodes. Expressed in B-cells and T-cells, expression increases during B-cell maturation and decreases during T-cell maturation.1 Publication

    Gene expression databases

    BgeeiO35906.
    CleanExiMM_SPIB.
    GenevestigatoriO35906.

    Interactioni

    Subunit structurei

    Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO35906.
    SMRiO35906. Positions 173-256.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3131TAD1 (Acidic)Add
    BLAST
    Regioni41 – 6222TAD2Add
    BLAST

    Domaini

    The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes By similarity.By similarity

    Sequence similaritiesi

    Belongs to the ETS family.Curated
    Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG241814.
    GeneTreeiENSGT00390000015212.
    HOGENOMiHOG000095520.
    HOVERGENiHBG002474.
    KOiK09439.
    OMAiATFSHPQ.
    OrthoDBiEOG78M02J.
    PhylomeDBiO35906.
    TreeFamiTF352494.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00178. Ets. 1 hit.
    [Graphical view]
    PRINTSiPR00454. ETSDOMAIN.
    SMARTiSM00413. ETS. 1 hit.
    [Graphical view]
    PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. Align

    Isoform IA (identifier: O35906-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLALEAAQLD GPHLSCLYPE GVFYDLDSCK PFSYPDSDGG LDSTWGWTEA    50
    PPAPAIAPYE AFDPATAAFS HSQTVQLCYS HGPNPSTYSP MGTLDPAPSL 100
    EAPGPGLQVY PPEDFTSQTL GSLAYAPYPS PVLSEEEDIM LDSPALEVSD 150
    SESDEALLAG SEGRGSEAGA RKKLRLYQFL LGLLLRGDMR ECVWWVEPGA 200
    GVFQFSSKHK ELLARRWGQQ KGNRKRMTYQ KLARALRNYA KTGEIRKVKR 250
    KLTYQFDSAL LPASRHV 267

    Note: Produced by alternative promoter usage.

    Length:267
    Mass (Da):29,365
    Last modified:June 21, 2005 - v2
    Checksum:iD32485A93C44327F
    GO
    Isoform IC (identifier: O35906-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         17-17: L → LVGEGVSPRPEMTLSSLRQ

    Note: Produced by alternative splicing of isoform IA. No experimental confirmation available.

    Show »
    Length:285
    Mass (Da):31,290
    Checksum:iAA7AC02B7E1AACC9
    GO
    Isoform IIA (identifier: O35906-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-8: MLALEAAQ → MPLCLSPAR

    Note: Produced by alternative promoter usage.

    Show »
    Length:268
    Mass (Da):29,506
    Checksum:i0FBAD29580730610
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 88MLALEAAQ → MPLCLSPAR in isoform IIA. CuratedVSP_013907
    Alternative sequencei17 – 171L → LVGEGVSPRPEMTLSSLRQ in isoform IC. CuratedVSP_013908

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87619
    , U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53554.1.
    U87619
    , U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53556.1.
    U87619
    , U87616, U87617, U87618 Genomic DNA. Translation: AAC53557.1.
    U87620 Genomic DNA. Translation: AAC53559.1.
    CCDSiCCDS21209.1. [O35906-1]
    RefSeqiNP_063919.1. NM_019866.1. [O35906-1]
    XP_006540996.1. XM_006540933.1. [O35906-2]
    XP_006540998.1. XM_006540935.1. [O35906-3]
    UniGeneiMm.8012.

    Genome annotation databases

    EnsembliENSMUST00000035323; ENSMUSP00000035539; ENSMUSG00000008193. [O35906-1]
    ENSMUST00000098483; ENSMUSP00000096084; ENSMUSG00000008193. [O35906-2]
    GeneIDi272382.
    KEGGimmu:272382.
    UCSCiuc009gpw.1. mouse. [O35906-1]

    Keywords - Coding sequence diversityi

    Alternative promoter usage, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U87619
    , U87614 , U87616 , U87617 , U87618 Genomic DNA. Translation: AAC53554.1 .
    U87619
    , U87614 , U87616 , U87617 , U87618 Genomic DNA. Translation: AAC53556.1 .
    U87619
    , U87616 , U87617 , U87618 Genomic DNA. Translation: AAC53557.1 .
    U87620 Genomic DNA. Translation: AAC53559.1 .
    CCDSi CCDS21209.1. [O35906-1 ]
    RefSeqi NP_063919.1. NM_019866.1. [O35906-1 ]
    XP_006540996.1. XM_006540933.1. [O35906-2 ]
    XP_006540998.1. XM_006540935.1. [O35906-3 ]
    UniGenei Mm.8012.

    3D structure databases

    ProteinModelPortali O35906.
    SMRi O35906. Positions 173-256.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    MaxQBi O35906.
    PaxDbi O35906.
    PRIDEi O35906.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035323 ; ENSMUSP00000035539 ; ENSMUSG00000008193 . [O35906-1 ]
    ENSMUST00000098483 ; ENSMUSP00000096084 ; ENSMUSG00000008193 . [O35906-2 ]
    GeneIDi 272382.
    KEGGi mmu:272382.
    UCSCi uc009gpw.1. mouse. [O35906-1 ]

    Organism-specific databases

    CTDi 6689.
    MGIi MGI:892986. Spib.

    Phylogenomic databases

    eggNOGi NOG241814.
    GeneTreei ENSGT00390000015212.
    HOGENOMi HOG000095520.
    HOVERGENi HBG002474.
    KOi K09439.
    OMAi ATFSHPQ.
    OrthoDBi EOG78M02J.
    PhylomeDBi O35906.
    TreeFami TF352494.

    Miscellaneous databases

    NextBioi 393578.
    PROi O35906.
    SOURCEi Search...

    Gene expression databases

    Bgeei O35906.
    CleanExi MM_SPIB.
    Genevestigatori O35906.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000418. Ets_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00178. Ets. 1 hit.
    [Graphical view ]
    PRINTSi PR00454. ETSDOMAIN.
    SMARTi SM00413. ETS. 1 hit.
    [Graphical view ]
    PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
    PS00346. ETS_DOMAIN_2. 1 hit.
    PS50061. ETS_DOMAIN_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two promoters direct expression of the murine Spi-B gene, an Ets family transcription factor."
      Chen H.-M., Gonzalez D.A., Radomska H.S., Voso M.T., Sun Z., Zhang P., Zhang D.-E., Tenen D.G.
      Gene 207:209-218(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS IA; IC AND IIA), ALTERNATIVE PROMOTER USAGE.
      Strain: 129/Sv.
    2. "The Ets protein Spi-B is expressed exclusively in B cells and T cells during development."
      Su G.H., Ip H.S., Cobb B.S., Lu M.-M., Chen H.-M., Simon M.C.
      J. Exp. Med. 184:203-214(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    3. "Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B."
      Su G.H., Chen H.-M., Muthusamy N., Garrett-Sinha L.A., Baunoch D., Tenen D.G., Simon M.C.
      EMBO J. 16:7118-7129(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction."
      Garrett-Sinha L.A., Su G.H., Rao S., Kabak S., Hao Z., Clark M.R., Simon M.C.
      Immunity 10:399-408(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1."
      Garrett-Sinha L.A., Dahl R., Rao S., Barton K.P., Simon M.C.
      Blood 97:2908-2912(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "PU.1/Spi-B regulation of c-rel is essential for mature B cell survival."
      Hu C.-J., Rao S., Ramirez-Bergeron D.L., Garrett-Sinha L.A., Gerondakis S., Clark M.R., Simon M.C.
      Immunity 15:545-555(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Spi-B can functionally replace PU.1 in myeloid but not lymphoid development."
      Dahl R., Ramirez-Bergeron D.L., Rao S., Simon M.C.
      EMBO J. 21:2220-2230(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "The transcription factor Spi-B is not required for somatic hypermutation."
      Kim N., Martin T.E., Simon M.C., Storb U.
      Mol. Immunol. 39:577-583(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSPIB_MOUSE
    AccessioniPrimary (citable) accession number: O35906
    Secondary accession number(s): O35907, O35909, O55199
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3