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O35906

- SPIB_MOUSE

UniProt

O35906 - SPIB_MOUSE

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Protein

Transcription factor Spi-B

Gene

Spib

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi174 – 25784ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. sequence-specific DNA binding Source: InterPro
  2. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: RefGenome
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. macrophage differentiation Source: MGI
  2. regulation of transcription, DNA-templated Source: MGI
  3. regulation of transcription from RNA polymerase II promoter Source: RefGenome
  4. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor Spi-B
Gene namesi
Name:Spib
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:892986. Spib.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: RefGenome
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Transcription factor Spi-BPRO_0000204137Add
BLAST

Proteomic databases

MaxQBiO35906.
PaxDbiO35906.
PRIDEiO35906.

Expressioni

Tissue specificityi

Expressed in the medulla of the thymus, the spleen and germinal centers of the lymph nodes. Expressed in B-cells and T-cells, expression increases during B-cell maturation and decreases during T-cell maturation.1 Publication

Gene expression databases

BgeeiO35906.
CleanExiMM_SPIB.
GenevestigatoriO35906.

Interactioni

Subunit structurei

Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO35906.
SMRiO35906. Positions 173-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3131TAD1 (Acidic)Add
BLAST
Regioni41 – 6222TAD2Add
BLAST

Domaini

The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes (By similarity).By similarity

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG241814.
GeneTreeiENSGT00390000015212.
HOGENOMiHOG000095520.
HOVERGENiHBG002474.
InParanoidiO35906.
KOiK09439.
OMAiATFSHPQ.
OrthoDBiEOG78M02J.
PhylomeDBiO35906.
TreeFamiTF352494.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative promoter usage and alternative splicing. Align

Isoform IA (identifier: O35906-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLALEAAQLD GPHLSCLYPE GVFYDLDSCK PFSYPDSDGG LDSTWGWTEA
60 70 80 90 100
PPAPAIAPYE AFDPATAAFS HSQTVQLCYS HGPNPSTYSP MGTLDPAPSL
110 120 130 140 150
EAPGPGLQVY PPEDFTSQTL GSLAYAPYPS PVLSEEEDIM LDSPALEVSD
160 170 180 190 200
SESDEALLAG SEGRGSEAGA RKKLRLYQFL LGLLLRGDMR ECVWWVEPGA
210 220 230 240 250
GVFQFSSKHK ELLARRWGQQ KGNRKRMTYQ KLARALRNYA KTGEIRKVKR
260
KLTYQFDSAL LPASRHV

Note: Produced by alternative promoter usage.

Length:267
Mass (Da):29,365
Last modified:June 21, 2005 - v2
Checksum:iD32485A93C44327F
GO
Isoform IC (identifier: O35906-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     17-17: L → LVGEGVSPRPEMTLSSLRQ

Note: Produced by alternative splicing of isoform IA. No experimental confirmation available.

Show »
Length:285
Mass (Da):31,290
Checksum:iAA7AC02B7E1AACC9
GO
Isoform IIA (identifier: O35906-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MLALEAAQ → MPLCLSPAR

Note: Produced by alternative promoter usage.

Show »
Length:268
Mass (Da):29,506
Checksum:i0FBAD29580730610
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88MLALEAAQ → MPLCLSPAR in isoform IIA. CuratedVSP_013907
Alternative sequencei17 – 171L → LVGEGVSPRPEMTLSSLRQ in isoform IC. CuratedVSP_013908

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87619
, U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53554.1.
U87619
, U87614, U87616, U87617, U87618 Genomic DNA. Translation: AAC53556.1.
U87619
, U87616, U87617, U87618 Genomic DNA. Translation: AAC53557.1.
U87620 Genomic DNA. Translation: AAC53559.1.
CCDSiCCDS21209.1. [O35906-1]
RefSeqiNP_063919.1. NM_019866.1. [O35906-1]
XP_006540996.1. XM_006540933.1. [O35906-2]
XP_006540998.1. XM_006540935.1. [O35906-3]
UniGeneiMm.8012.

Genome annotation databases

EnsembliENSMUST00000035323; ENSMUSP00000035539; ENSMUSG00000008193. [O35906-1]
ENSMUST00000098483; ENSMUSP00000096084; ENSMUSG00000008193. [O35906-2]
GeneIDi272382.
KEGGimmu:272382.
UCSCiuc009gpw.1. mouse. [O35906-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U87619
, U87614 , U87616 , U87617 , U87618 Genomic DNA. Translation: AAC53554.1 .
U87619
, U87614 , U87616 , U87617 , U87618 Genomic DNA. Translation: AAC53556.1 .
U87619
, U87616 , U87617 , U87618 Genomic DNA. Translation: AAC53557.1 .
U87620 Genomic DNA. Translation: AAC53559.1 .
CCDSi CCDS21209.1. [O35906-1 ]
RefSeqi NP_063919.1. NM_019866.1. [O35906-1 ]
XP_006540996.1. XM_006540933.1. [O35906-2 ]
XP_006540998.1. XM_006540935.1. [O35906-3 ]
UniGenei Mm.8012.

3D structure databases

ProteinModelPortali O35906.
SMRi O35906. Positions 173-256.
ModBasei Search...
MobiDBi Search...

Proteomic databases

MaxQBi O35906.
PaxDbi O35906.
PRIDEi O35906.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000035323 ; ENSMUSP00000035539 ; ENSMUSG00000008193 . [O35906-1 ]
ENSMUST00000098483 ; ENSMUSP00000096084 ; ENSMUSG00000008193 . [O35906-2 ]
GeneIDi 272382.
KEGGi mmu:272382.
UCSCi uc009gpw.1. mouse. [O35906-1 ]

Organism-specific databases

CTDi 6689.
MGIi MGI:892986. Spib.

Phylogenomic databases

eggNOGi NOG241814.
GeneTreei ENSGT00390000015212.
HOGENOMi HOG000095520.
HOVERGENi HBG002474.
InParanoidi O35906.
KOi K09439.
OMAi ATFSHPQ.
OrthoDBi EOG78M02J.
PhylomeDBi O35906.
TreeFami TF352494.

Miscellaneous databases

NextBioi 393578.
PROi O35906.
SOURCEi Search...

Gene expression databases

Bgeei O35906.
CleanExi MM_SPIB.
Genevestigatori O35906.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000418. Ets_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00178. Ets. 1 hit.
[Graphical view ]
PRINTSi PR00454. ETSDOMAIN.
SMARTi SM00413. ETS. 1 hit.
[Graphical view ]
PROSITEi PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Two promoters direct expression of the murine Spi-B gene, an Ets family transcription factor."
    Chen H.-M., Gonzalez D.A., Radomska H.S., Voso M.T., Sun Z., Zhang P., Zhang D.-E., Tenen D.G.
    Gene 207:209-218(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS IA; IC AND IIA), ALTERNATIVE PROMOTER USAGE.
    Strain: 129/Sv.
  2. "The Ets protein Spi-B is expressed exclusively in B cells and T cells during development."
    Su G.H., Ip H.S., Cobb B.S., Lu M.-M., Chen H.-M., Simon M.C.
    J. Exp. Med. 184:203-214(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B."
    Su G.H., Chen H.-M., Muthusamy N., Garrett-Sinha L.A., Baunoch D., Tenen D.G., Simon M.C.
    EMBO J. 16:7118-7129(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "PU.1 and Spi-B are required for normal B cell receptor-mediated signal transduction."
    Garrett-Sinha L.A., Su G.H., Rao S., Kabak S., Hao Z., Clark M.R., Simon M.C.
    Immunity 10:399-408(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1."
    Garrett-Sinha L.A., Dahl R., Rao S., Barton K.P., Simon M.C.
    Blood 97:2908-2912(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "PU.1/Spi-B regulation of c-rel is essential for mature B cell survival."
    Hu C.-J., Rao S., Ramirez-Bergeron D.L., Garrett-Sinha L.A., Gerondakis S., Clark M.R., Simon M.C.
    Immunity 15:545-555(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Spi-B can functionally replace PU.1 in myeloid but not lymphoid development."
    Dahl R., Ramirez-Bergeron D.L., Rao S., Simon M.C.
    EMBO J. 21:2220-2230(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The transcription factor Spi-B is not required for somatic hypermutation."
    Kim N., Martin T.E., Simon M.C., Storb U.
    Mol. Immunol. 39:577-583(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPIB_MOUSE
AccessioniPrimary (citable) accession number: O35906
Secondary accession number(s): O35907, O35909, O55199
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: October 29, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3