Reviewed, UniProtKB/Swiss-Prot O35904 (PK3CD_MOUSE)

Names and origin

Protein names

Recommended name:
    Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform
      Short name=PtdIns-3-kinase subunit delta
      Short name=PI3-kinase subunit delta
      Short name=PI3K-delta
    EC=2.7.1.153
Alternative name(s):
    Phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta
      Short name=PtdIns-3-kinase subunit p110-delta

Gene names

Name:

Pik3cd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1043 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.
Pathway	Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.
Subunit structure	Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Interacts with ERAS. Ref.2
Tissue specificity	Expressed predominantly in leukocytes.
Post-translational modification	Autophosphorylation on Ser-1038 results in the almost complete inactivation of the lipid kinase activity By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 PI3K/PI4K domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	B cell activation Inferred from mutant phenotype. Source: MGI B cell homeostasis Inferred from mutant phenotype. Source: MGI cell surface receptor linked signal transduction Inferred from mutant phenotype. Source: MGI phosphoinositide phosphorylation Inferred from electronic annotation. Source: InterPro phosphoinositide-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	phosphoinositide 3-kinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	1-phosphatidylinositol-3-kinase activity Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylinositol-4,5-bisphosphate 3-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1043	1043	Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta isoform		PRO_0000088791
Regions
	Domain	775 – 1028	254	PI3K/PI4K
Amino acid modifications
	Modified residue	484	1	Phosphotyrosine By similarity
	Modified residue	519	1	Phosphoserine By similarity
	Modified residue	523	1	Phosphotyrosine By similarity
	Modified residue	1038	1	Phosphoserine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35904-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A998F9E092CA361D	FASTA	1,043	119,647
	10 20 30 40 50 60 MPPGVDCPME FWTKEESQSV VVDFLLPTGV YLNFPVSRNA NLSTIKQVLW HRAQYEPLFH 70 80 90 100 110 120 MLSDPEAYVF TCVNQTAEQQ ELEDEQRRLC DIQPFLPVLR LVAREGDRVK KLINSQISLL 130 140 150 160 170 180 IAKGLHEFDS LRDPEVNDFR TKMRQFCEEA AAHRQQLGWV EWLQYSFPLQ LEPSARGWRA 190 200 210 220 230 240 GLLRVSNRAL LVNVKFEGSE ESFTFQVSTK DMPLALMACA LRKKATVFRQ PLVEQPEEYA 250 260 270 280 290 300 LQVNGRHEYL YGNYPLCHFQ YICSCLHSGL TPHLTMVHSS SILAMRDEQS NPAPQVQKPR 310 320 330 340 350 360 AKPPPIPAKK PSSVSLWSLE QPFSIELIEG RKVNADERMK LVVQAGLFHG NEMLCKTVSS 370 380 390 400 410 420 SEVNVCSEPV WKQRLEFDIS VCDLPRMARL CFALYAVVEK AKKARSTKKK SKKADCPIAW 430 440 450 460 470 480 ANLMLFDYKD QLKTGERCLY MWPSVPDEKG ELLNPAGTVR GNPNTESAAA LVIYLPEVAP 490 500 510 520 530 540 HPVYFPALEK ILELGRHGER GRITEEELQL REILERRGSG ELYEHEKDLV WKMRHEVQEH 550 560 570 580 590 600 FPEALARLLL VTKWNKHEDV AQMLYLLCSW PELPVLSALE LLDSSFPDCY VGSFAIKSLR 610 620 630 640 650 660 KLTDDELFQY LLQLVQVLKY ESYLDCELTK FLLGRALANR KIGHFLFWHL HSEMHVPSVA 670 680 690 700 710 720 LRFGLIMEAY CRGSTHHMKV LMKQGEALSK LKALNDFVKV SSQKTTKPQT KEMMHMCMRQ 730 740 750 760 770 780 ETYMEALSHL QSPLDPSTLL EEVCVEQCTF MDSKMKPLWI MYSSEEAGSA GNVGIIFKNG 790 800 810 820 830 840 DDLRQDMLTL QMIQLMDVLW KQEGLDLRMT PYGCLPTGDR TGLIEVVLHS DTIANIQLNK 850 860 870 880 890 900 SNMAATAAFN KDALLNWLKS KNPGEALDRA IEEFTLSCAG YCVATYVLGI GDRHSDNIMI 910 920 930 940 950 960 RESGQLFHID FGHFLGNFKT KFGINRERVP FILTYDFVHV IQQGKTNNSE KFERFRGYCE 970 980 990 1000 1010 1020 RAYTILRRHG LLFLHLFALM RAAGLPELSC SKDIQYLKDS LALGKTEEEA LKHFRVKFNE 1030 1040 ALRESWKTKV NWLAHNVSKD NRQ

References

[1]	"p110delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes." Chantry D., Vojtek A., Kashishian A., Holtzman D.A., Wood C., Gray P.W., Cooper J.A., Hoekstra M.F. J. Biol. Chem. 272:19236-19241(1997) [PubMed: 9235916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[2]	"Role of Eras in promoting tumor-like properties in mouse embryonic stem cells." Takahashi K., Mitsui K., Yamanaka S. Nature 423:541-545(2003) [PubMed: 12774123] [Abstract] Cited for: INTERACTION WITH ERAS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U86587 mRNA. Translation: AAC25676.1.
IPI	IPI00885697.
PIR	T43502.
UniGene	Mm.229108
3D structure databases
SMR	O35904. Positions 110-1038.
ModBase	Search...
Protein-protein interaction databases
IntAct	O35904. 2 interactions.
STRING	O35904.
PTM databases
PhosphoSite	O35904.
Proteomic databases
PRIDE	O35904.
Genome annotation databases
Ensembl	ENSMUST00000105689; ENSMUSP00000101314; ENSMUSG00000039936; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:1098211. Pik3cd.
Phylogenomic databases
eggNOG	roNOG11071.
HOVERGEN	O35904.
Enzyme and pathway databases
BRENDA	2.7.1.153. 244.
Gene expression databases
ArrayExpress	O35904.
Bgee	O35904.
Genevestigator	O35904.
GermOnline	ENSMUSG00000039936. Mus musculus.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2. IPR003113. PI3K_p85_bd. IPR000341. PI3K_ras_bd. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit. G3DSA:1.25.40.70. PI3Ka. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF02192. PI3K_p85B. 1 hit. PF00794. PI3K_rbd. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
SMART	SM00142. PI3K_C2. 1 hit. SM00143. PI3K_p85B. 1 hit. SM00144. PI3K_rbd. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PK3CD_MOUSE

Accession

Primary (citable) accession number: O35904

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)