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Protein

Nuclear autoantigen Sp-100

Gene

Sp100

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Together with PML, this tumor suppressor is a major constituent of the PML bodies, a subnuclear organelle involved in a large number of physiological processes including cell growth, differentiation and apoptosis. Functions as a transcriptional coactivator of ETS1 and ETS2. Under certain conditions, it may also act as a corepressor of ETS1 preventing its binding to DNA. Through the regulation of ETS1 it may play a role in angiogenesis, controlling endothelial cell motility and invasion. Through interaction with the MRN complex it may be involved in the regulation of telomeres lengthening. May also regulate TP53-mediated transcription and through CASP8AP2, regulate FAS-mediated apoptosis. May also play a role in infection by viruses through mechanisms that may involve chromatin and/or transcriptional regulation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear autoantigen Sp-100
Alternative name(s):
Nuclear dot-associated Sp100 protein
Speckled 100 kDa
Gene namesi
Name:Sp100
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:109561. Sp100.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation
  • NucleusPML body By similarity
  • Cytoplasm By similarity

  • Note: Accumulates in the cytoplasm upon FAS activation.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 482482Nuclear autoantigen Sp-100PRO_0000074098Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei174 – 1741PhosphoserineCombined sources
Modified residuei190 – 1901PhosphoserineCombined sources
Modified residuei209 – 2091PhosphothreonineCombined sources
Cross-linki243 – 243Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei313 – 3131PhosphothreonineCombined sources
Modified residuei314 – 3141PhosphoserineCombined sources
Modified residuei316 – 3161PhosphothreonineCombined sources
Modified residuei318 – 3181PhosphoserineCombined sources
Modified residuei319 – 3191PhosphoserineCombined sources

Post-translational modificationi

Sumoylated. Sumoylated with SUMO1. Sumoylation depends on a functional nuclear localization signal but is not necessary for nuclear import or nuclear body targeting. Sumoylation may stabilize the interaction with CBX5 (By similarity).By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO35892.
MaxQBiO35892.
PaxDbiO35892.
PeptideAtlasiO35892.
PRIDEiO35892.

PTM databases

iPTMnetiO35892.
PhosphoSiteiO35892.

Expressioni

Inductioni

By interferon.

Gene expression databases

BgeeiENSMUSG00000026222.
CleanExiMM_SP100.
ExpressionAtlasiO35892. baseline and differential.
GenevisibleiO35892. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with members of the HP1 family of nonhistone chromosomal protein, such as CBX5 and CBX3 via the PxVxL motif. Interacts with ETS1; the interaction is direct and modulates ETS1 transcriptional activity. Interacts with the MRN complex which is composed of two heterodimers RAD50/MRE11A associated with a single NBN; recruits the complex to PML-related bodies. Interacts with HIPK2; positively regulates TP53-dependent transcription. Interacts with CASP8AP2; may negatively regulate CASP8AP2 export from the nucleus to the cytoplasm (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066399.

Structurei

3D structure databases

ProteinModelPortaliO35892.
SMRiO35892. Positions 378-467.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 121116HSRPROSITE-ProRule annotationAdd
BLAST
Domaini378 – 45982SANDPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi230 – 24314PxVxL motifAdd
BLAST

Domaini

The HSR domain is important for the nuclear body targeting as well as for the dimerization.By similarity
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.

Sequence similaritiesi

Contains 1 HSR domain.PROSITE-ProRule annotation
Contains 1 SAND domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00510000046835.
HOGENOMiHOG000089984.
HOVERGENiHBG062077.
InParanoidiO35892.
KOiK15413.

Family and domain databases

Gene3Di3.10.390.10. 1 hit.
InterProiIPR004865. HSR_dom.
IPR000770. SAND_dom.
IPR010919. SAND_dom-like.
[Graphical view]
PfamiPF01342. SAND. 1 hit.
PF03172. Sp100. 1 hit.
[Graphical view]
SMARTiSM00258. SAND. 1 hit.
[Graphical view]
SUPFAMiSSF63763. SSF63763. 1 hit.
PROSITEiPS51414. HSR. 1 hit.
PS50864. SAND. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35892-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEGSDGSPRM STEQENTEMH LIECMLKHFK TQKVAISNAI RSTFPFLESL
60 70 80 90 100
RDHEFITGKM YEDLLDSCRS LVPVDKVIYR ALEELEKKFD MTVLCELFNE
110 120 130 140 150
VNMEKYPDLN LIRRSFGCVF PNELCFQGID GGNPNSQLSL EQGPGASYSQ
160 170 180 190 200
GSPNGSSLDL SASEGWRSND RRNSNLMQAN QTENHQLAES PGHLDSCELQ
210 220 230 240 250
VQLNNRDATP ESCSLLPQNE ERAVQLNYEL QINPCFVQLV DVKKENSSFS
260 270 280 290 300
LAGNQQTRAR TNQNEDSEII ELSSGDSDNG ENFSEATTTV PSQPAPAYSR
310 320 330 340 350
KPPTLRRDRG GDTSDTESSI IIRRRKRTGR KKRERLGSYL IRNIKIPMKP
360 370 380 390 400
SWKTAFLARS ANPSSQRRRK RGPRIPREEN ADFGGAELPV VCGNAQGFLD
410 420 430 440 450
KEKFKQGIYV RSIRGKTGRL FTPMDFEIEG NCEKAKNWRQ SIRCKGWTLR
460 470 480
ELIQKGVLQD PPRKKKETPR NPRQTRRQVN AL
Length:482
Mass (Da):54,727
Last modified:July 27, 2011 - v2
Checksum:i4F41E481AE655412
GO
Isoform 2 (identifier: O35892-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     312-330: DTSDTESSIIIRRRKRTGR → G

Show »
Length:464
Mass (Da):52,554
Checksum:iD3F64225E0396654
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831E → D in AAC40174 (PubMed:9268632).Curated
Sequence conflicti91 – 922MT → IP in AAC40174 (PubMed:9268632).Curated
Sequence conflicti114 – 1141R → K in AAC40174 (PubMed:9268632).Curated
Sequence conflicti117 – 1171G → E in AAC40174 (PubMed:9268632).Curated
Sequence conflicti131 – 1311G → R in AAC53512 (PubMed:9268632).Curated
Sequence conflicti131 – 1311G → R in AAC53513 (PubMed:9268632).Curated
Sequence conflicti204 – 2063NNR → ITE in AAC40172 (PubMed:9268632).Curated
Sequence conflicti441 – 4411S → T in AAC53512 (PubMed:9268632).Curated
Sequence conflicti441 – 4411S → T in AAC53513 (PubMed:9268632).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei312 – 33019DTSDT…KRTGR → G in isoform 2. 1 PublicationVSP_005985Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83630 mRNA. Translation: AAC53512.1.
U83636 mRNA. Translation: AAC53513.1.
AF040242, AF040241 Genomic DNA. Translation: AAC40172.1.
AF038850 Genomic DNA. Translation: AAC40174.1.
AC147806 Genomic DNA. No translation available.
AC161342 Genomic DNA. No translation available.
RefSeqiNP_001300641.1. NM_001313712.1. [O35892-1]
NP_001300642.1. NM_001313713.1. [O35892-2]
UniGeneiMm.290906.
Mm.486514.

Genome annotation databases

EnsembliENSMUST00000147552; ENSMUSP00000116942; ENSMUSG00000026222. [O35892-2]
ENSMUST00000155094; ENSMUSP00000118481; ENSMUSG00000026222. [O35892-1]
GeneIDi20684.
KEGGimmu:20684.
UCSCiuc007buf.1. mouse. [O35892-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U83630 mRNA. Translation: AAC53512.1.
U83636 mRNA. Translation: AAC53513.1.
AF040242, AF040241 Genomic DNA. Translation: AAC40172.1.
AF038850 Genomic DNA. Translation: AAC40174.1.
AC147806 Genomic DNA. No translation available.
AC161342 Genomic DNA. No translation available.
RefSeqiNP_001300641.1. NM_001313712.1. [O35892-1]
NP_001300642.1. NM_001313713.1. [O35892-2]
UniGeneiMm.290906.
Mm.486514.

3D structure databases

ProteinModelPortaliO35892.
SMRiO35892. Positions 378-467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066399.

PTM databases

iPTMnetiO35892.
PhosphoSiteiO35892.

Proteomic databases

EPDiO35892.
MaxQBiO35892.
PaxDbiO35892.
PeptideAtlasiO35892.
PRIDEiO35892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000147552; ENSMUSP00000116942; ENSMUSG00000026222. [O35892-2]
ENSMUST00000155094; ENSMUSP00000118481; ENSMUSG00000026222. [O35892-1]
GeneIDi20684.
KEGGimmu:20684.
UCSCiuc007buf.1. mouse. [O35892-1]

Organism-specific databases

CTDi6672.
MGIiMGI:109561. Sp100.

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00510000046835.
HOGENOMiHOG000089984.
HOVERGENiHBG062077.
InParanoidiO35892.
KOiK15413.

Enzyme and pathway databases

ReactomeiR-MMU-3108214. SUMOylation of DNA damage response and repair proteins.

Miscellaneous databases

ChiTaRSiSp100. mouse.
PROiO35892.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026222.
CleanExiMM_SP100.
ExpressionAtlasiO35892. baseline and differential.
GenevisibleiO35892. MM.

Family and domain databases

Gene3Di3.10.390.10. 1 hit.
InterProiIPR004865. HSR_dom.
IPR000770. SAND_dom.
IPR010919. SAND_dom-like.
[Graphical view]
PfamiPF01342. SAND. 1 hit.
PF03172. Sp100. 1 hit.
[Graphical view]
SMARTiSM00258. SAND. 1 hit.
[Graphical view]
SUPFAMiSSF63763. SSF63763. 1 hit.
PROSITEiPS51414. HSR. 1 hit.
PS50864. SAND. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSP100_MOUSE
AccessioniPrimary (citable) accession number: O35892
Secondary accession number(s): E9PY02
, O35897, O88392, O88395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Sp100 is a single-copy gene. A related gene, D1LUB1/Sp100-rs, occurs in multiple copies and forms a conspicuous cluster in the chromosome 1. Sp100 and D1LUB1/Sp100-rs are homologous from the promoter up to a position in intron 3, but they differ 3' of that position.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.