Reviewed, UniProtKB/Swiss-Prot O35885 (ASCL2_MOUSE)

Names and origin

Protein names

Recommended name:
    Achaete-scute homolog 2
      Short name=ASH-2
      Short name=mASH-2
      Short name=mASH2

Gene names

Name:	Ascl2
Synonyms:	Mash2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system By similarity.
Subunit structure	Efficient DNA binding requires dimerization with another bHLH protein By similarity.
Subcellular location	Nucleus.
Sequence similarities	Contains 1 basic helix-loop-helix (bHLH) domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Developmental protein
Gene Ontology (GO)
Biological process	cell differentiation Inferred from mutant phenotype. Source: MGI in utero embryonic development Inferred from mutant phenotype. Source: MGI nervous system development Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Achaete-scute homolog 2		PRO_0000127131
Regions
	Domain	132 – 171	40	Helix-loop-helix motif
	DNA binding	121 – 131	11	Basic motif
	Compositional bias	104 – 107	4	Poly-Arg
	Compositional bias	114 – 117	4	Poly-Ser
Experimental info
	Sequence conflict	119	1	A → S in AAB86992. Ref.1
	Sequence conflict	179	1	A → T in AAB86992. Ref.1
	Sequence conflict	182	1	A → P in AAB86992. Ref.1
	Sequence conflict	200	1	A → T in AAB86992. Ref.1
	Sequence conflict	209	1	A → G in AAB86992. Ref.1
	Sequence conflict	220 – 222	3	PDR → RT in AAB86992. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O35885-1 [UniParc]. Last modified March 25, 2003. Version 2. Checksum: 9FA907B436E1BA2E	FASTA	263	27,784
	10 20 30 40 50 60 MEAHLDWYGV PGLQEASDAC PRESCSSALP EAREGANVHF PPHPVPREHF SCAAPELVAG 70 80 90 100 110 120 AQGLNASLMD GGALPRLMPT SSGVAGACAA RRRQASPELL RCSRRRRSGA TEASSSSAAV 130 140 150 160 170 180 ARRNERERNR VKLVNLGFQA LRQHVPHGGA NKKLSKVETL RSAVEYIRAL QRLLAEHDAV 190 200 210 220 230 240 RAALAGGLLT PATPPSDECA QPSASPASAS LSCASTSPSP DRLGCSEPTS PRSAYSSEES 250 260 SCEGELSPME QELLDFSSWL GGY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human Achaete-Scute homologue 2 (ASCL2,HASH2) maps to chromosome 11p15.5, close to IGF2 and is expressed in extravillus trophoblasts." Alders M., Hodges M., Hadjantonakis A.-K., Postmus J., van Wijk I.J., Bliek J., de Meulemeester M., Westerveld A., Guillemot F., Oudejans C.B., Little P., Mannens M. Hum. Mol. Genet. 6:859-867(1997) [PubMed: 9175731] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Parental origin-specific expression of Mash2 is established at the time of implantation with its imprinting mechanism highly resistant to genome-wide demethylation." Tanaka M., Puchyr M., Gertsenstein M., Harpal K., Jaenisch R., Rossant J., Nagy A. Mech. Dev. 87:129-142(1999) [PubMed: 10495277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence conservation and variability of imprinting in the Beckwith-Wiedemann syndrome gene cluster in human and mouse." Paulsen M., El-Maarri O., Engemann S., Stroedicke M., Franck O., Davies K., Reinhardt R., Reik W., Walter J. Hum. Mol. Genet. 9:1829-1841(2000) [PubMed: 10915772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U77628 Genomic DNA. Translation: AAB86992.1. AF139595 Genomic DNA. Translation: AAD33794.1. AJ251835 Genomic DNA. Translation: CAB94773.1. BC019520 mRNA. Translation: AAH19520.1.
IPI	IPI00135184.
RefSeq	NP_032580.2.
UniGene	Mm.196417
3D structure databases
SMR	O35885. Positions 123-176.
ModBase	Search...
Proteomic databases
PRIDE	O35885.
Genome annotation databases
Ensembl	ENSMUST00000009392; ENSMUSP00000009392; ENSMUSG00000009248; Mus musculus. [Genome view]
GeneID	17173.
KEGG	mmu:17173.
UCSC	uc009koj.1. mouse.
Organism-specific databases
CTD	17173.
MGI	MGI:96920. Ascl2.
Phylogenomic databases
eggNOG	roNOG08572.
HOGENOM	HBG283332.
HOVERGEN	O35885.
InParanoid	O35885.
OMA	EAREGAN.
OrthoDB	EOG95QKZQ.
PhylomeDB	O35885.
Gene expression databases
ArrayExpress	O35885.
Bgee	O35885.
CleanEx	MM_ASCL2.
Genevestigator	O35885.
GermOnline	ENSMUSG00000009248. Mus musculus.
Family and domain databases
InterPro	IPR015660. ASH. IPR001092. HLH_DNA-bd_dom. IPR011598. HLH_DNA_bd. [Graphical view]
Gene3D	G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit.
PANTHER	PTHR13935. ASH. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
PROSITE	PS50888. HLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	291472.
SOURCE	Search...

Entry information

Entry name

ASCL2_MOUSE

Accession

Primary (citable) accession number: O35885
Secondary accession number(s): Q9WUJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
Last sequence update:	March 25, 2003
Last modified:	February 9, 2010
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)