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Protein

Neutral amino acid transporter A

Gene

Slc1a4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence (By similarity).By similarity

GO - Molecular functioni

  1. chloride channel activity Source: MGI
  2. L-alanine transmembrane transporter activity Source: MGI
  3. L-cystine transmembrane transporter activity Source: Ensembl
  4. L-hydroxyproline transmembrane transporter activity Source: MGI
  5. L-proline transmembrane transporter activity Source: MGI
  6. L-serine transmembrane transporter activity Source: MGI
  7. L-threonine transmembrane transporter activity Source: MGI
  8. neutral amino acid transmembrane transporter activity Source: MGI
  9. sodium:dicarboxylate symporter activity Source: InterPro

GO - Biological processi

  1. chloride transmembrane transport Source: MGI
  2. chloride transport Source: MGI
  3. cognition Source: MGI
  4. hydroxyproline transport Source: MGI
  5. L-alanine transport Source: MGI
  6. L-serine transport Source: MGI
  7. proline transmembrane transport Source: MGI
  8. proline transport Source: MGI
  9. threonine transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral amino acid transporter A
Alternative name(s):
Alanine/serine/cysteine/threonine transporter 1
Short name:
ASCT-1
SATT
Solute carrier family 1 member 4
Gene namesi
Name:Slc1a4
Synonyms:Asct1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2135601. Slc1a4.

Subcellular locationi

Membrane Curated; Multi-pass membrane protein Curated. Melanosome By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4141CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei42 – 6221HelicalSequence AnalysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Transmembranei119 – 13921HelicalSequence AnalysisAdd
BLAST
Topological domaini140 – 21677ExtracellularSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Transmembranei257 – 27721HelicalSequence AnalysisAdd
BLAST
Transmembranei298 – 31821HelicalSequence AnalysisAdd
BLAST
Transmembranei328 – 34821HelicalSequence AnalysisAdd
BLAST
Transmembranei373 – 39321HelicalSequence AnalysisAdd
BLAST
Transmembranei418 – 43821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: MGI
  2. centrosome Source: MGI
  3. dendrite Source: Ensembl
  4. extracellular vesicular exosome Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
  6. intermediate filament Source: MGI
  7. melanosome Source: UniProtKB-SubCell
  8. membrane Source: MGI
  9. microtubule organizing center Source: MGI
  10. neuronal cell body Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Neutral amino acid transporter APRO_0000202080Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi177 – 1771N-linked (GlcNAc...); atypical1 Publication
Glycosylationi182 – 1821N-linked (GlcNAc...); atypical1 Publication
Glycosylationi201 – 2011N-linked (GlcNAc...)2 Publications
Glycosylationi206 – 2061N-linked (GlcNAc...)2 Publications
Modified residuei507 – 5071PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO35874.
PaxDbiO35874.
PRIDEiO35874.

PTM databases

PhosphoSiteiO35874.

Expressioni

Gene expression databases

BgeeiO35874.
ExpressionAtlasiO35874. baseline and differential.
GenevestigatoriO35874.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105223.

Structurei

3D structure databases

ProteinModelPortaliO35874.
SMRiO35874. Positions 51-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1301.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO35874.
KOiK05615.
OMAiGVVCRLC.
PhylomeDBiO35874.
TreeFamiTF315206.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKSGETNGY LDGTQAEPAA GPRTPETAMG KSQRCASFFR RHALVLLTVS
60 70 80 90 100
GVLVGAGMGA ALRGLQLTRT QITYLAFPGE MLLRMLRMII LPLVVCSLVS
110 120 130 140 150
GAASLDASSL GRLGGIAVAY FGLTTLSASA LAVALAFIIK PGAGAQTLQS
160 170 180 190 200
SSLGLENSGP PPVSKETVDS FLDLLRNLFP SNLVVAAFTT SATDYTVVTH
210 220 230 240 250
NTSSGNVTKE KIPVVTDVEG MNILGLVLFA LVLGVALKKL GPEGEDLIRF
260 270 280 290 300
FNSFNEATMV LVSWIMWYVP IGIMFLIGSK IVEMKDIVML VTSLGKYIFA
310 320 330 340 350
SMLGHVIHGG IVLPLVYFAF TRKNPFTFLL GLLTPFATAF ATCSSSATLP
360 370 380 390 400
SMMKCIEENN GVDKRISRFI LPIGATVNMD GAAIFQCVAA VFIAQLNNVD
410 420 430 440 450
LNAGQIFTIL VTATASSVGA AGVPAGGVLT IAIILEAIGL PTHDLSLILA
460 470 480 490 500
VDWIVDRTTT VVNVEGDALG AGILNHLNQK VVKKGEQELQ EVKVEAIPNS
510 520 530
KSEEETSPLV THQNPAGPVA IAPELESKES VL
Length:532
Mass (Da):56,062
Last modified:December 31, 1997 - v1
Checksum:i99DD9E43C5DB55D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75215 mRNA. Translation: AAB71740.1.
BC052733 mRNA. Translation: AAH52733.1.
CCDSiCCDS24457.1.
RefSeqiNP_061349.3. NM_018861.3.
UniGeneiMm.6379.

Genome annotation databases

EnsembliENSMUST00000109594; ENSMUSP00000105223; ENSMUSG00000020142.
GeneIDi55963.
KEGGimmu:55963.
UCSCiuc007idb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U75215 mRNA. Translation: AAB71740.1.
BC052733 mRNA. Translation: AAH52733.1.
CCDSiCCDS24457.1.
RefSeqiNP_061349.3. NM_018861.3.
UniGeneiMm.6379.

3D structure databases

ProteinModelPortaliO35874.
SMRiO35874. Positions 51-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000105223.

PTM databases

PhosphoSiteiO35874.

Proteomic databases

MaxQBiO35874.
PaxDbiO35874.
PRIDEiO35874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109594; ENSMUSP00000105223; ENSMUSG00000020142.
GeneIDi55963.
KEGGimmu:55963.
UCSCiuc007idb.2. mouse.

Organism-specific databases

CTDi6509.
MGIiMGI:2135601. Slc1a4.

Phylogenomic databases

eggNOGiCOG1301.
HOGENOMiHOG000208776.
HOVERGENiHBG000080.
InParanoidiO35874.
KOiK05615.
OMAiGVVCRLC.
PhylomeDBiO35874.
TreeFamiTF315206.

Enzyme and pathway databases

ReactomeiREACT_301178. Amino acid transport across the plasma membrane.

Miscellaneous databases

ChiTaRSiSlc1a4. mouse.
NextBioi311666.
PROiO35874.
SOURCEiSearch...

Gene expression databases

BgeeiO35874.
ExpressionAtlasiO35874. baseline and differential.
GenevestigatoriO35874.

Family and domain databases

Gene3Di1.10.3860.10. 2 hits.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 2 hits.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Peng J.-B., Guo L.-H.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-177; ASN-182; ASN-201 AND ASN-206.
    Tissue: Myoblast.
  5. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-201 AND ASN-206.

Entry informationi

Entry nameiSATT_MOUSE
AccessioniPrimary (citable) accession number: O35874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1998
Last sequence update: December 31, 1997
Last modified: March 31, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.