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O35874 (SATT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neutral amino acid transporter A
Alternative name(s):
Alanine/serine/cysteine/threonine transporter 1
Short name=ASCT-1
SATT
Solute carrier family 1 member 4
Gene names
Name:Slc1a4
Synonyms:Asct1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transporter for alanine, serine, cysteine, and threonine. Exhibits sodium dependence By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Probable. Melanosome By similarity.

Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A4 subfamily. [View classification]

Ontologies

Keywords
   Biological processSymport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcognition

Inferred from electronic annotation. Source: Compara

   Cellular_componentdendrite

Inferred from electronic annotation. Source: Compara

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intermediate filament

Inferred from direct assay PubMed 12533615. Source: MGI

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuronal cell body

Inferred from electronic annotation. Source: Compara

   Molecular_functionL-alanine transmembrane transporter activity

Inferred from electronic annotation. Source: Compara

L-cystine transmembrane transporter activity

Inferred from electronic annotation. Source: Compara

L-hydroxyproline transmembrane transporter activity

Inferred from electronic annotation. Source: Compara

L-proline transmembrane transporter activity

Inferred from electronic annotation. Source: Compara

L-serine transmembrane transporter activity

Inferred from electronic annotation. Source: Compara

L-threonine transmembrane transporter activity

Inferred from electronic annotation. Source: Compara

chloride channel activity

Inferred from electronic annotation. Source: Compara

neutral amino acid transmembrane transporter activity

Inferred from direct assay PubMed 10933718. Source: MGI

sodium:dicarboxylate symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 532532Neutral amino acid transporter A
PRO_0000202080

Regions

Topological domain1 – 4141Cytoplasmic Potential
Transmembrane42 – 6221Helical; Potential
Transmembrane88 – 10821Helical; Potential
Transmembrane119 – 13921Helical; Potential
Topological domain140 – 21677Extracellular Potential
Transmembrane217 – 23721Helical; Potential
Transmembrane257 – 27721Helical; Potential
Transmembrane298 – 31821Helical; Potential
Transmembrane328 – 34821Helical; Potential
Transmembrane373 – 39321Helical; Potential
Transmembrane418 – 43821Helical; Potential

Amino acid modifications

Modified residue5061Phosphothreonine Ref.3
Modified residue5071Phosphoserine Ref.3
Glycosylation1771N-linked (GlcNAc...) Ref.4
Glycosylation1821N-linked (GlcNAc...) Ref.4
Glycosylation2011N-linked (GlcNAc...) Ref.4 Ref.5
Glycosylation2061N-linked (GlcNAc...) Ref.4 Ref.5

Sequences

Sequence LengthMass (Da)Tools
O35874 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 99DD9E43C5DB55D7

FASTA53256,062
        10         20         30         40         50         60 
MEKSGETNGY LDGTQAEPAA GPRTPETAMG KSQRCASFFR RHALVLLTVS GVLVGAGMGA 

        70         80         90        100        110        120 
ALRGLQLTRT QITYLAFPGE MLLRMLRMII LPLVVCSLVS GAASLDASSL GRLGGIAVAY 

       130        140        150        160        170        180 
FGLTTLSASA LAVALAFIIK PGAGAQTLQS SSLGLENSGP PPVSKETVDS FLDLLRNLFP 

       190        200        210        220        230        240 
SNLVVAAFTT SATDYTVVTH NTSSGNVTKE KIPVVTDVEG MNILGLVLFA LVLGVALKKL 

       250        260        270        280        290        300 
GPEGEDLIRF FNSFNEATMV LVSWIMWYVP IGIMFLIGSK IVEMKDIVML VTSLGKYIFA 

       310        320        330        340        350        360 
SMLGHVIHGG IVLPLVYFAF TRKNPFTFLL GLLTPFATAF ATCSSSATLP SMMKCIEENN 

       370        380        390        400        410        420 
GVDKRISRFI LPIGATVNMD GAAIFQCVAA VFIAQLNNVD LNAGQIFTIL VTATASSVGA 

       430        440        450        460        470        480 
AGVPAGGVLT IAIILEAIGL PTHDLSLILA VDWIVDRTTT VVNVEGDALG AGILNHLNQK 

       490        500        510        520        530 
VVKKGEQELQ EVKVEAIPNS KSEEETSPLV THQNPAGPVA IAPELESKES VL

« Hide

References

« Hide 'large scale' references
[1]Peng J.-B., Guo L.-H.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-506 AND SER-507, MASS SPECTROMETRY.
Tissue: Brain cortex.
[4]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-177; ASN-182; ASN-201 AND ASN-206, MASS SPECTROMETRY.
Tissue: Myoblast.
[5]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-201 AND ASN-206, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U75215 mRNA. Translation: AAB71740.1.
BC052733 mRNA. Translation: AAH52733.1.
IPIIPI00135130.
RefSeqNP_061349.3. NM_018861.3.
UniGeneMm.6379.

3D structure databases

ProteinModelPortalO35874.
SMRO35874. Positions 51-474.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000105223.

PTM databases

PhosphoSiteO35874.

Proteomic databases

PaxDbO35874.
PRIDEO35874.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000109594; ENSMUSP00000105223; ENSMUSG00000020142.
GeneID55963.
KEGGmmu:55963.

Organism-specific databases

CTD6509.
MGIMGI:2135601. Slc1a4.

Phylogenomic databases

eggNOGCOG1301.
HOGENOMHOG000208776.
HOVERGENHBG000080.
InParanoidO35874.
KOK05615.
OMAALATDMK.

Gene expression databases

ArrayExpressO35874.
BgeeO35874.
GenevestigatorO35874.
GermOnlineENSMUSG00000020142. Mus musculus.

Family and domain databases

InterProIPR001991. Na-dicarboxylate_symporter.
IPR018107. Na-dicarboxylate_symporter_CS.
[Graphical view]
PANTHERPTHR11958. PTHR11958. 1 hit.
PfamPF00375. SDF. 1 hit.
[Graphical view]
PRINTSPR00173. EDTRNSPORT.
PROSITEPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
PS00714. NA_DICARBOXYL_SYMP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC1A4. mouse.
NextBio311666.
SOURCESearch...

Entry information

Entry nameSATT_MOUSE
AccessionPrimary (citable) accession number: O35874
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents