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Protein

Mitochondrial import inner membrane translocase subunit TIM44

Gene

Timm44

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi166 – 1738ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM44
Gene namesi
Name:Timm44
Synonyms:Mimt44, Tim44
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1343262. Timm44.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrial matrix Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 452Mitochondrial import inner membrane translocase subunit TIM44PRO_0000034315
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei177 – 1771N6-succinyllysineCombined sources
Modified residuei180 – 1801PhosphoserineBy similarity
Modified residuei217 – 2171N6-succinyllysineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35857.
MaxQBiO35857.
PaxDbiO35857.
PRIDEiO35857.

2D gel databases

REPRODUCTION-2DPAGEO35857.

PTM databases

iPTMnetiO35857.
PhosphoSiteiO35857.
SwissPalmiO35857.

Expressioni

Gene expression databases

BgeeiO35857.
CleanExiMM_TIMM44.
GenevisibleiO35857. MM.

Interactioni

Subunit structurei

Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19. The complex interacts with the TIMM23 component of the TIM23 complex (By similarity).By similarity

Protein-protein interaction databases

MINTiMINT-1845288.
STRINGi10090.ENSMUSP00000003029.

Structurei

3D structure databases

ProteinModelPortaliO35857.
SMRiO35857. Positions 270-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim44 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2580. Eukaryota.
ENOG410Y5IK. LUCA.
GeneTreeiENSGT00390000000051.
HOGENOMiHOG000013004.
HOVERGENiHBG055113.
InParanoidiO35857.
KOiK17804.
OMAiKQCENDI.
OrthoDBiEOG7H792G.
TreeFamiTF106197.

Family and domain databases

InterProiIPR032710. NTF2-like_dom.
IPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view]
PfamiPF04280. Tim44. 1 hit.
[Graphical view]
PIRSFiPIRSF037871. TIM44. 1 hit.
SMARTiSM00978. Tim44. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
TIGRFAMsiTIGR00984. 3a0801s03tim44. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAALRGGW CRCPRRCLGS GIQFLSSHNL PHGSSYQISR PGRELTLTKS
60 70 80 90 100
YSSGSRKGFL SGLLDNIKQE LAKNKEMKES IKKFRDEAKK LEESDALQEA
110 120 130 140 150
RRKYKSIESE TVRTSEAIKK KLGELTGTVK ESLDEVSKSD LGRKIKEGVE
160 170 180 190 200
EAARTAKQSA ESVSKSGEKL GKTAAFKAIS QGVESVKKEL DESVLGQTGP
210 220 230 240 250
YRRPERLRKR TEFAGAKFKE SKVFEANEEA LGVVLHKDSK WYQQWKDFKD
260 270 280 290 300
NNVVFNRFFE MKMKYDESDN VLIRASRALT DKVTDLLGGL FSKTEMSEVL
310 320 330 340 350
TEILRVDPTF DKDHFLHQCE TDIIPNILEA MISGELDILK DWCYEATYSQ
360 370 380 390 400
LAHPIQQAKA LGFQFHSRIL DISNVDLAMG KMMEQGPVLI VTFQAQVVMV
410 420 430 440 450
IKNSKGEVYD GDPDKVQRML YVWALCRDQE ELNPYAAWRL LDISASSTEQ

IL
Length:452
Mass (Da):51,091
Last modified:July 27, 2011 - v2
Checksum:iDC3E5F4E43972D2F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51A → R in AAB97624 (PubMed:9419343).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69898 mRNA. Translation: AAB97624.1.
CH466566 Genomic DNA. Translation: EDL21992.1.
BC110677 mRNA. Translation: AAI10678.1.
BC117523 mRNA. Translation: AAI17524.1.
BC117524 mRNA. Translation: AAI17525.1.
CCDSiCCDS22083.1.
RefSeqiNP_035722.2. NM_011592.2.
UniGeneiMm.195249.

Genome annotation databases

EnsembliENSMUST00000003029; ENSMUSP00000003029; ENSMUSG00000002949.
GeneIDi21856.
KEGGimmu:21856.
UCSCiuc009ktq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69898 mRNA. Translation: AAB97624.1.
CH466566 Genomic DNA. Translation: EDL21992.1.
BC110677 mRNA. Translation: AAI10678.1.
BC117523 mRNA. Translation: AAI17524.1.
BC117524 mRNA. Translation: AAI17525.1.
CCDSiCCDS22083.1.
RefSeqiNP_035722.2. NM_011592.2.
UniGeneiMm.195249.

3D structure databases

ProteinModelPortaliO35857.
SMRiO35857. Positions 270-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1845288.
STRINGi10090.ENSMUSP00000003029.

PTM databases

iPTMnetiO35857.
PhosphoSiteiO35857.
SwissPalmiO35857.

2D gel databases

REPRODUCTION-2DPAGEO35857.

Proteomic databases

EPDiO35857.
MaxQBiO35857.
PaxDbiO35857.
PRIDEiO35857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003029; ENSMUSP00000003029; ENSMUSG00000002949.
GeneIDi21856.
KEGGimmu:21856.
UCSCiuc009ktq.2. mouse.

Organism-specific databases

CTDi10469.
MGIiMGI:1343262. Timm44.

Phylogenomic databases

eggNOGiKOG2580. Eukaryota.
ENOG410Y5IK. LUCA.
GeneTreeiENSGT00390000000051.
HOGENOMiHOG000013004.
HOVERGENiHBG055113.
InParanoidiO35857.
KOiK17804.
OMAiKQCENDI.
OrthoDBiEOG7H792G.
TreeFamiTF106197.

Miscellaneous databases

ChiTaRSiTimm44. mouse.
NextBioi301346.
PROiO35857.
SOURCEiSearch...

Gene expression databases

BgeeiO35857.
CleanExiMM_TIMM44.
GenevisibleiO35857. MM.

Family and domain databases

InterProiIPR032710. NTF2-like_dom.
IPR017303. Tim44.
IPR007379. Tim44-like_dom.
[Graphical view]
PfamiPF04280. Tim44. 1 hit.
[Graphical view]
PIRSFiPIRSF037871. TIM44. 1 hit.
SMARTiSM00978. Tim44. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
TIGRFAMsiTIGR00984. 3a0801s03tim44. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mammalian translocase of inner mitochondrial membrane (Tim44) isolated from diabetic newborn mouse kidney."
    Wada J., Kanwar Y.S.
    Proc. Natl. Acad. Sci. U.S.A. 95:144-149(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD-1.
    Tissue: Kidney.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NMRI.
    Tissue: Mammary tumor.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-177 AND LYS-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTIM44_MOUSE
AccessioniPrimary (citable) accession number: O35857
Secondary accession number(s): Q2NLC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.