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Protein

Branched-chain-amino-acid aminotransferase, mitochondrial

Gene

Bcat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

GO - Molecular functioni

GO - Biological processi

  • aspartate biosynthetic process Source: GO_Central
  • branched-chain amino acid catabolic process Source: MGI
  • branched-chain amino acid metabolic process Source: MGI
  • isoleucine catabolic process Source: MGI
  • isoleucine metabolic process Source: MGI
  • leucine biosynthetic process Source: GO_Central
  • leucine metabolic process Source: MGI
  • regulation of hormone levels Source: MGI
  • valine biosynthetic process Source: GO_Central
  • valine metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase, mitochondrial (EC:2.6.1.42)
Short name:
BCAT(m)
Gene namesi
Name:Bcat2
Synonyms:Bcatm, Eca40
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1276534. Bcat2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionBy similarityAdd
BLAST
Chaini28 – 393366Branched-chain-amino-acid aminotransferase, mitochondrialPRO_0000001272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301N6-(pyridoxal phosphate)lysineBy similarity
Modified residuei322 – 3221N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO35855.
PaxDbiO35855.
PeptideAtlasiO35855.
PRIDEiO35855.

2D gel databases

REPRODUCTION-2DPAGEO35855.

PTM databases

iPTMnetiO35855.
PhosphoSiteiO35855.
SwissPalmiO35855.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030826.
CleanExiMM_BCAT2.
ExpressionAtlasiO35855. baseline and differential.
GenevisibleiO35855. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiO35855. 2 interactions.
MINTiMINT-4089160.
STRINGi10090.ENSMUSP00000033098.

Structurei

3D structure databases

ProteinModelPortaliO35855.
SMRiO35855. Positions 30-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiO35855.
KOiK00826.
OMAiKAHEWML.
OrthoDBiEOG091G07KO.
PhylomeDBiO35855.
TreeFamiTF300882.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATLGQVW ARKLLPVPWL LCGSKRCVSS IFKAADLQIQ MTKEPQKKPA
60 70 80 90 100
PSQALLFGKT FTDHMLMVEW NNKAGWGPPR IQPFQNLTLH PACSGLHYSL
110 120 130 140 150
QLFEGLKAYK GGDQQVRLFR PWLNMDRMLR SARRLCLPDF DKQELLECIR
160 170 180 190 200
QLIEVDKDWV PDGNGTSLYV RPVLIGNEPS LGVGMVTQAL LYVILCPVGS
210 220 230 240 250
YFPGDSMTPV SLLADPSFVR AWIGGVGDCK LGGNYGPTVA VQREAQKRGC
260 270 280 290 300
EQVLWLYGPD HQLTEVGTMN IFVYWTHEDG VLELVTPPLN GVILPGVVRQ
310 320 330 340 350
SLLDLARTWG EFRVAERKVT MKELKRALEE GRVREVFGSG TACQVCPVHQ
360 370 380 390
ILYEGKQLHI PTMENGPELI LRFQKELKAI QYGASAHDWM FRV
Length:393
Mass (Da):44,127
Last modified:August 16, 2004 - v2
Checksum:i7D2ACF7C743B1978
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti199 – 1991G → A in AAB67674 (PubMed:9165094).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017688 mRNA. Translation: AAH17688.1.
BC048072 mRNA. Translation: AAH48072.1.
U68526 mRNA. Translation: AAB67674.1.
CCDSiCCDS21252.1.
RefSeqiNP_033867.1. NM_009737.3.
UniGeneiMm.24210.

Genome annotation databases

EnsembliENSMUST00000033098; ENSMUSP00000033098; ENSMUSG00000030826.
GeneIDi12036.
KEGGimmu:12036.
UCSCiuc009gwc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017688 mRNA. Translation: AAH17688.1.
BC048072 mRNA. Translation: AAH48072.1.
U68526 mRNA. Translation: AAB67674.1.
CCDSiCCDS21252.1.
RefSeqiNP_033867.1. NM_009737.3.
UniGeneiMm.24210.

3D structure databases

ProteinModelPortaliO35855.
SMRiO35855. Positions 30-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35855. 2 interactions.
MINTiMINT-4089160.
STRINGi10090.ENSMUSP00000033098.

PTM databases

iPTMnetiO35855.
PhosphoSiteiO35855.
SwissPalmiO35855.

2D gel databases

REPRODUCTION-2DPAGEO35855.

Proteomic databases

EPDiO35855.
PaxDbiO35855.
PeptideAtlasiO35855.
PRIDEiO35855.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033098; ENSMUSP00000033098; ENSMUSG00000030826.
GeneIDi12036.
KEGGimmu:12036.
UCSCiuc009gwc.2. mouse.

Organism-specific databases

CTDi587.
MGIiMGI:1276534. Bcat2.

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiO35855.
KOiK00826.
OMAiKAHEWML.
OrthoDBiEOG091G07KO.
PhylomeDBiO35855.
TreeFamiTF300882.

Enzyme and pathway databases

ReactomeiR-MMU-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

PROiO35855.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030826.
CleanExiMM_BCAT2.
ExpressionAtlasiO35855. baseline and differential.
GenevisibleiO35855. MM.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCAT2_MOUSE
AccessioniPrimary (citable) accession number: O35855
Secondary accession number(s): Q8VD03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 16, 2004
Last modified: September 7, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.