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Protein

Branched-chain-amino-acid aminotransferase, mitochondrial

Gene

Bcat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

GO - Molecular functioni

GO - Biological processi

  • branched-chain amino acid catabolic process Source: MGI
  • branched-chain amino acid metabolic process Source: MGI
  • cellular response to leukemia inhibitory factor Source: MGI
  • isoleucine catabolic process Source: MGI
  • isoleucine metabolic process Source: MGI
  • leucine biosynthetic process Source: GO_Central
  • leucine metabolic process Source: MGI
  • regulation of hormone levels Source: MGI
  • valine biosynthetic process Source: GO_Central
  • valine metabolic process Source: MGI

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-70895 Branched-chain amino acid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase, mitochondrial (EC:2.6.1.42)
Short name:
BCAT(m)
Gene namesi
Name:Bcat2
Synonyms:Bcatm, Eca40
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1276534 Bcat2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000000127228 – 393Branched-chain-amino-acid aminotransferase, mitochondrialAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei322N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO35855
PaxDbiO35855
PeptideAtlasiO35855
PRIDEiO35855

2D gel databases

REPRODUCTION-2DPAGEiO35855

PTM databases

iPTMnetiO35855
PhosphoSitePlusiO35855
SwissPalmiO35855

Expressioni

Gene expression databases

BgeeiENSMUSG00000030826
CleanExiMM_BCAT2
ExpressionAtlasiO35855 baseline and differential
GenevisibleiO35855 MM

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiO35855, 2 interactors
MINTiO35855
STRINGi10090.ENSMUSP00000033098

Structurei

3D structure databases

ProteinModelPortaliO35855
SMRiO35855
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0975 Eukaryota
COG0115 LUCA
GeneTreeiENSGT00390000009532
HOGENOMiHOG000276704
HOVERGENiHBG050678
InParanoidiO35855
KOiK00826
OMAiKGWGQPR
OrthoDBiEOG091G07KO
PhylomeDBiO35855
TreeFamiTF300882

Family and domain databases

CDDicd01557 BCAT_beta_family, 1 hit
InterProiView protein in InterPro
IPR001544 Aminotrans_IV
IPR018300 Aminotrans_IV_CS
IPR036038 Aminotransferase-like
IPR005786 B_amino_transII
IPR033939 BCAT_family
PfamiView protein in Pfam
PF01063 Aminotran_4, 1 hit
PIRSFiPIRSF006468 BCAT1, 1 hit
SUPFAMiSSF56752 SSF56752, 1 hit
TIGRFAMsiTIGR01123 ilvE_II, 1 hit
PROSITEiView protein in PROSITE
PS00770 AA_TRANSFER_CLASS_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAATLGQVW ARKLLPVPWL LCGSKRCVSS IFKAADLQIQ MTKEPQKKPA
60 70 80 90 100
PSQALLFGKT FTDHMLMVEW NNKAGWGPPR IQPFQNLTLH PACSGLHYSL
110 120 130 140 150
QLFEGLKAYK GGDQQVRLFR PWLNMDRMLR SARRLCLPDF DKQELLECIR
160 170 180 190 200
QLIEVDKDWV PDGNGTSLYV RPVLIGNEPS LGVGMVTQAL LYVILCPVGS
210 220 230 240 250
YFPGDSMTPV SLLADPSFVR AWIGGVGDCK LGGNYGPTVA VQREAQKRGC
260 270 280 290 300
EQVLWLYGPD HQLTEVGTMN IFVYWTHEDG VLELVTPPLN GVILPGVVRQ
310 320 330 340 350
SLLDLARTWG EFRVAERKVT MKELKRALEE GRVREVFGSG TACQVCPVHQ
360 370 380 390
ILYEGKQLHI PTMENGPELI LRFQKELKAI QYGASAHDWM FRV
Length:393
Mass (Da):44,127
Last modified:August 16, 2004 - v2
Checksum:i7D2ACF7C743B1978
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti199G → A in AAB67674 (PubMed:9165094).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017688 mRNA Translation: AAH17688.1
BC048072 mRNA Translation: AAH48072.1
U68526 mRNA Translation: AAB67674.1
CCDSiCCDS21252.1
RefSeqiNP_033867.1, NM_009737.3
UniGeneiMm.24210

Genome annotation databases

EnsembliENSMUST00000033098; ENSMUSP00000033098; ENSMUSG00000030826
GeneIDi12036
KEGGimmu:12036
UCSCiuc009gwc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiBCAT2_MOUSE
AccessioniPrimary (citable) accession number: O35855
Secondary accession number(s): Q8VD03
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 16, 2004
Last modified: February 28, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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