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Protein

Branched-chain-amino-acid aminotransferase, mitochondrial

Gene

Bcat2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.1 Publication

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

GO - Molecular functioni

GO - Biological processi

  • aspartate biosynthetic process Source: GO_Central
  • branched-chain amino acid biosynthetic process Source: UniProtKB-KW
  • branched-chain amino acid catabolic process Source: RGD
  • branched-chain amino acid metabolic process Source: RGD
  • isoleucine catabolic process Source: RGD
  • lactation Source: RGD
  • leucine biosynthetic process Source: RGD
  • valine biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

SABIO-RKO35854.

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase, mitochondrial (EC:2.6.1.42)
Short name:
BCAT(m)
Gene namesi
Name:Bcat2
Synonyms:Bcatm, Eca40
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68948. Bcat2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • mitochondrion Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3319.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionAdd BLAST27
ChainiPRO_000000127428 – 393Branched-chain-amino-acid aminotransferase, mitochondrialAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei230N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei322N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO35854.
PRIDEiO35854.

PTM databases

iPTMnetiO35854.
PhosphoSitePlusiO35854.

Expressioni

Tissue specificityi

Expressed in all tissues.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiO35854. 1 interactor.
STRINGi10116.ENSRNOP00000028474.

Chemistry databases

BindingDBiO35854.

Structurei

3D structure databases

ProteinModelPortaliO35854.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiO35854.
KOiK00826.
PhylomeDBiO35854.

Family and domain databases

CDDicd01557. BCAT_beta_family. 1 hit.
InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
IPR033939. BCAT_family.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35854-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAILGQVW TRKLLPIPWR LCVPGRCVSS NFKAADLQVQ VTREPQKKPA
60 70 80 90 100
PSQPLLFGKT FTDHMLMVEW NSKTGWGPPR IQPFQNLTLH PACSGLHYSL
110 120 130 140 150
QLFEGLKAYK GRDKQVRLFR PWLNMDRMLR SARRLCLPDF DKQELLECIR
160 170 180 190 200
QLIEVDKDWV PDGNGTSLYV RPVLIGNEPS LGVGMVTQAL LFVILCPVGS
210 220 230 240 250
YFPGDSMTPV SLLADPSFVR AWIGGVGDCK LGGNYGPTVA VQQEAQKKGC
260 270 280 290 300
EQVLWLYGPD HQLTEVGTMN IFVYWTHEDG ELELATPPLD GIILPGVVRQ
310 320 330 340 350
SLLDLARTWG EFRVAERKVT MKELKRALEE GRVREVFGSG TACQVCPVHQ
360 370 380 390
ILYEGKQLHI PTMENGPELI LRFQKELKAI QYGTSAHDWM LRV
Length:393
Mass (Da):44,276
Last modified:January 1, 1998 - v1
Checksum:iECB3704ED49A6C12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68417 mRNA. Translation: AAB67673.1.
RefSeqiNP_071795.1. NM_022400.1.
UniGeneiRn.981.

Genome annotation databases

GeneIDi64203.
KEGGirno:64203.
UCSCiRGD:68948. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68417 mRNA. Translation: AAB67673.1.
RefSeqiNP_071795.1. NM_022400.1.
UniGeneiRn.981.

3D structure databases

ProteinModelPortaliO35854.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35854. 1 interactor.
STRINGi10116.ENSRNOP00000028474.

Chemistry databases

BindingDBiO35854.
ChEMBLiCHEMBL3319.

PTM databases

iPTMnetiO35854.
PhosphoSitePlusiO35854.

Proteomic databases

PaxDbiO35854.
PRIDEiO35854.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64203.
KEGGirno:64203.
UCSCiRGD:68948. rat.

Organism-specific databases

CTDi587.
RGDi68948. Bcat2.

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiO35854.
KOiK00826.
PhylomeDBiO35854.

Enzyme and pathway databases

SABIO-RKO35854.

Miscellaneous databases

PROiO35854.

Family and domain databases

CDDicd01557. BCAT_beta_family. 1 hit.
InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
IPR033939. BCAT_family.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBCAT2_RAT
AccessioniPrimary (citable) accession number: O35854
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.