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Protein

Apoptosis inhibitor 5

Gene

Api5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis inhibitor 5
Short name:
API-5
Alternative name(s):
AAC-11
Gene namesi
Name:Api5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1888993. Api5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • spliceosomal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000646352 – 504Apoptosis inhibitor 5Add BLAST503

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251N6-acetyllysineBy similarity1
Modified residuei399PhosphothreonineBy similarity1
Modified residuei462PhosphoserineBy similarity1
Modified residuei464PhosphoserineCombined sources1
Modified residuei469PhosphoserineCombined sources1

Post-translational modificationi

Acetylation at Lys-251 impairs antiapoptotic function.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO35841.
MaxQBiO35841.
PaxDbiO35841.
PeptideAtlasiO35841.
PRIDEiO35841.

PTM databases

iPTMnetiO35841.
PhosphoSitePlusiO35841.

Expressioni

Inductioni

Down-regulated in vascular smooth muscle cells (vSMCs) treated with benzo[a]pyrene (BaP).1 Publication

Gene expression databases

BgeeiENSMUSG00000027193.
CleanExiMM_API5.
GenevisibleiO35841. MM.

Interactioni

Subunit structurei

Monomer. Interacts with FGF2 and ACIN1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO35841. 1 interactor.
MINTiMINT-1866852.
STRINGi10090.ENSMUSP00000028617.

Structurei

3D structure databases

ProteinModelPortaliO35841.
SMRiO35841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 360ARM-like and Heat-like helical repeatsBy similarityAdd BLAST359
Regioni370 – 391Leucine-zipperAdd BLAST22

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi454 – 475Nuclear localization signalBy similarityAdd BLAST22

Sequence similaritiesi

Belongs to the API5 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2213. Eukaryota.
ENOG410XSH0. LUCA.
GeneTreeiENSGT00390000010991.
HOVERGENiHBG050542.
InParanoidiO35841.
OMAiNRSRGRI.
OrthoDBiEOG091G06NK.
TreeFamiTF324283.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR008383. API5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF05918. API5. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVEELYRN YGILADATEQ VGQHKDAYQV ILDGVKGGTK EKRLAAQFIP
60 70 80 90 100
KFFKHFPELA DSAINAQLDL CEDEDVSIRR QAIKELPQFA TGENLPRVAD
110 120 130 140 150
ILTQLLQTDD SAEFNLVNNA LLSIFKMDAK GTLGGLFSQI LQGEDIVRER
160 170 180 190 200
AIKFLSTKLK TLPDEVLTKE VEELILTESK KVLEDVTGEE FVLFMKILSG
210 220 230 240 250
LKSLQTVSGR QQLVELVAEQ ADLEQAFSPS DPDCVDRLLQ CTRQAVPLFS
260 270 280 290 300
KNVHSTRFVT YFCEQVLPNL STLTTPVEGL DIQLEVLKLL AEMSSFCGDM
310 320 330 340 350
EKLETNLRKL FDKLLEYMPL PPEEAENGEN AGNEEPKLQF SYVECLLYSF
360 370 380 390 400
HQLGRKLPDF LTAKLNAEKL KDFKIRLQYF ARGLQVYIRQ LRLALQGKTG
410 420 430 440 450
EALKTEENKI KVVALKITNN INVLIKDLFH IPPSYKSTVT LSWKPVQKVE
460 470 480 490 500
IGQKRTSEDT SSGSPPKKSP GGPKRDARQI YNPPSGKYSS NLSNFNYERS

LQGK
Length:504
Mass (Da):56,785
Last modified:July 27, 2011 - v2
Checksum:i11E4CC89509487B9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti288K → N in AAB86526 (PubMed:9307294).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35846 mRNA. Translation: AAB86526.1.
AK137466 mRNA. Translation: BAE23364.1.
AK153934 mRNA. Translation: BAE32263.1.
AK167375 mRNA. Translation: BAE39470.1.
AL672251 Genomic DNA. Translation: CAM16211.1.
CH466519 Genomic DNA. Translation: EDL27645.1.
BC007133 mRNA. Translation: AAH07133.1.
CCDSiCCDS16459.1.
RefSeqiNP_031492.2. NM_007466.3.
UniGeneiMm.181824.
Mm.486651.

Genome annotation databases

EnsembliENSMUST00000028617; ENSMUSP00000028617; ENSMUSG00000027193.
GeneIDi11800.
KEGGimmu:11800.
UCSCiuc008lgx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35846 mRNA. Translation: AAB86526.1.
AK137466 mRNA. Translation: BAE23364.1.
AK153934 mRNA. Translation: BAE32263.1.
AK167375 mRNA. Translation: BAE39470.1.
AL672251 Genomic DNA. Translation: CAM16211.1.
CH466519 Genomic DNA. Translation: EDL27645.1.
BC007133 mRNA. Translation: AAH07133.1.
CCDSiCCDS16459.1.
RefSeqiNP_031492.2. NM_007466.3.
UniGeneiMm.181824.
Mm.486651.

3D structure databases

ProteinModelPortaliO35841.
SMRiO35841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35841. 1 interactor.
MINTiMINT-1866852.
STRINGi10090.ENSMUSP00000028617.

PTM databases

iPTMnetiO35841.
PhosphoSitePlusiO35841.

Proteomic databases

EPDiO35841.
MaxQBiO35841.
PaxDbiO35841.
PeptideAtlasiO35841.
PRIDEiO35841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028617; ENSMUSP00000028617; ENSMUSG00000027193.
GeneIDi11800.
KEGGimmu:11800.
UCSCiuc008lgx.2. mouse.

Organism-specific databases

CTDi8539.
MGIiMGI:1888993. Api5.

Phylogenomic databases

eggNOGiKOG2213. Eukaryota.
ENOG410XSH0. LUCA.
GeneTreeiENSGT00390000010991.
HOVERGENiHBG050542.
InParanoidiO35841.
OMAiNRSRGRI.
OrthoDBiEOG091G06NK.
TreeFamiTF324283.

Miscellaneous databases

ChiTaRSiApi5. mouse.
PROiO35841.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027193.
CleanExiMM_API5.
GenevisibleiO35841. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR008383. API5.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
[Graphical view]
PfamiPF05918. API5. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPI5_MOUSE
AccessioniPrimary (citable) accession number: O35841
Secondary accession number(s): Q3U517, Q922L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.