O35832 (CKD18_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 18
EC=2.7.11.22

Alternative name(s):
Cell division protein kinase 18
PCTAIRE-motif protein kinase 3
Serine/threonine-protein kinase PCTAIRE-3

Gene names

Name:	Cdk18
Synonyms:	Pctaire3, Pctk3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in signal transduction cascades in terminally differentiated cells.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	In brain, kidney, intestine and at a much lower level, in fetal tissues.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Cyclin-dependent kinase 18		PRO_0000086493
Regions
	Domain	121 – 402	282	Protein kinase
	Nucleotide binding	127 – 135	9	ATP By similarity
Sites
	Active site	242	1	Proton acceptor By similarity
	Binding site	150	1	ATP By similarity
Amino acid modifications
	Modified residue	12	1	Phosphoserine By similarity
	Modified residue	51	1	Phosphoserine By similarity
	Modified residue	66	1	Phosphoserine Ref.2
	Modified residue	75	1	Phosphoserine By similarity
	Modified residue	109	1	Phosphoserine Ref.2
	Modified residue	111	1	Phosphoserine Ref.2
	Modified residue	134	1	Phosphothreonine By similarity
	Modified residue	449	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O35832 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: C3E4688F51B142E8	FASTA	451	51,882
	10 20 30 40 50 60 MNKMKNFKRR LSLSVPRPET IEESLTEFTE QFNQLHTQRN EDGRDEPGQL SPGVQYQQRQ 70 80 90 100 110 120 NQRRFSMEDL NKRLSLPMDI RLPQEFLQKL QLENPGLPKP LTRMSRRASL SDIGFGKLET 130 140 150 160 170 180 YVKLDKLGEG TYATVFKGRS KLTENLVALK EIRLEHEEGA PCTAIREVSL LKDLKHANIV 190 200 210 220 230 240 TLHDLIHTDR SLTLVFEYLD SDLKQYLDHC GNLMNMHNVK IFMFQLLRGL AYCHRRKILH 250 260 270 280 290 300 RDLKPQNLLI NERGELKLAD FGLARAKSVP TKTYSNEVVT LWYRPPDVLL GSTEYSTPID 310 320 330 340 350 360 MWGVGCILYE MATGKPLFPG STVKEELHLI FRLLGTPTEE SWPGVTSISE FRAYNFPRYL 370 380 390 400 410 420 PQPLLSHAPR LDTEGINLLT SLLLYESKSR MSAEAALSHP YFQSLGERVH QLDDTASIFS 430 440 450 LKEIQLQKDP GYRGLAFQHP GRGKSRRQSI F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PCTAIRE 2, a Cdc2-related serine/threonine kinase, is predominantly expressed in terminally differentiated neurons." Hirose T., Tamaru T., Okumura N., Nagai K., Okada M. Eur. J. Biochem. 249:481-488(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites." Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A. Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-109 AND SER-111, MASS SPECTROMETRY. Tissue: Renal collecting duct.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB005541 mRNA. Translation: BAA21472.1.
IPI	IPI00371321.
RefSeq	NP_001093976.1. NM_001100506.1.
UniGene	Rn.219420.
3D structure databases
ProteinModelPortal	O35832.
SMR	O35832. Positions 121-404.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000010976.
PTM databases
PhosphoSite	O35832.
Proteomic databases
PaxDb	O35832.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000010976; ENSRNOP00000010976; ENSRNOG00000008137.
GeneID	289019.
KEGG	rno:289019.
UCSC	RGD:1309523. rat.
Organism-specific databases
CTD	5129.
RGD	1309523. Cdk18.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
HOVERGEN	HBG014652.
InParanoid	O35832.
KO	K15596.
OMA	HTDRSLT.
OrthoDB	EOG47D9FX.
Enzyme and pathway databases
BRENDA	2.7.11.22. 5301.
Gene expression databases
ArrayExpress	O35832.
Genevestigator	O35832.
GermOnline	ENSRNOG00000008137. Rattus norvegicus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	629076.

Entry information

Entry name

CKD18_RAT

Accession

Primary (citable) accession number: O35832

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program