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Protein

A-kinase anchor protein 14

Gene

Akap14

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to type II regulatory subunits of protein kinase A and anchors/targets them.

GO - Molecular functioni

  • protein kinase A binding Source: RGD

GO - Biological processi

  • spermatogenesis Source: RGD
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 14
Short name:
AKAP-14
Alternative name(s):
TAKAP-1.2
Testis-specific A-kinase-anchoring protein TAKAP-80
Gene namesi
Name:Akap14
Synonyms:Takap80
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi708510. Akap14.

Subcellular locationi

  • Cytoplasm Curated

  • Note: In fibrous sheath from epididymal sperm.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • sperm fibrous sheath Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502A-kinase anchor protein 14PRO_0000064520Add
BLAST

Proteomic databases

PaxDbiO35817.
PRIDEiO35817.

Expressioni

Tissue specificityi

Testis specific.1 Publication

Developmental stagei

Expressed at detectable levels 30 days after birth and increased greatly during the next 10 days.

Gene expression databases

GenevisibleiO35817. RN.

Interactioni

Subunit structurei

Binds to type II regulatory subunits (RII).

GO - Molecular functioni

  • protein kinase A binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009123.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati199 – 20241
Repeati205 – 20842
Repeati211 – 21443
Repeati217 – 22044
Repeati235 – 23845
Repeati241 – 24446
Repeati250 – 25347
Repeati262 – 26548

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 265678 X 4 AA repeat of P-X-X-PAdd
BLAST
Regioni258 – 378121RII-bindingAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 147144Asp/Glu/Lys-richAdd
BLAST
Compositional biasi148 – 267120Ala/Pro/Val-richAdd
BLAST
Compositional biasi268 – 36295Glu/Lys-richAdd
BLAST

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IXR2. Eukaryota.
ENOG4111RMU. LUCA.
GeneTreeiENSGT00390000003444.
HOGENOMiHOG000033875.
HOVERGENiHBG080826.
InParanoidiO35817.
KOiK16530.
OMAiIRFREQW.
OrthoDBiEOG73FQNR.
TreeFamiTF329057.

Family and domain databases

InterProiIPR025663. AKAP_28.
[Graphical view]
PfamiPF14469. AKAP28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNKKKPPNQ KAKTVENTST AKAKKPEAPE PKNVTEPKDS KAVEDPKKSV
60 70 80 90 100
TNQKEKKTVT IIADPKDKKS VTEPKEKKTV TISDPKDKKP VIEKDKRTVT
110 120 130 140 150
FVTDVKDKRI VTDTKENRIA SIDTKEKGPV VPEVKEKKVQ LIDPKEKVVA
160 170 180 190 200
PVAPVAPVAH AAQVPHAAQV PHAVQVPHAV PVAHAAPVAH TAQVSHAAPV
210 220 230 240 250
APSLPVVPPA PVVPLAPVVP PAPVAHTEPV AHAVPFAPPA PVAPPASVAP
260 270 280 290 300
PVPDAPVALV APVAPQVKQK IRVSEVKRKK EVPPRVPQLK EMKVVLFTPE
310 320 330 340 350
IKEKKVVSEI KEKTQVIDGK EKLIVPDVKE KKPAVVEKKD ERMNEIARTV
360 370 380 390 400
VEGVLAASVQ FVEEARNPIK NIKWLTHGEF TAEKGRKQIE KFVSTWEFQN
410 420 430 440 450
RWVYYADFIE KKDLIHSFHY IYRVRWSAPT AVRPMARVSA NAYFTIKFNK
460 470 480 490 500
SKPADMPVDV SYIFENSELL QRPGKIRFRE QWLRDITETK HILLESIPFK

VV
Length:502
Mass (Da):55,756
Last modified:January 1, 1998 - v1
Checksum:iD06DED22F954E975
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002474 mRNA. Translation: CAA05485.1.
RefSeqiNP_067735.1. NM_021703.1.
UniGeneiRn.42933.

Genome annotation databases

EnsembliENSRNOT00000009123; ENSRNOP00000009123; ENSRNOG00000006899.
GeneIDi60332.
KEGGirno:60332.
UCSCiRGD:708510. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002474 mRNA. Translation: CAA05485.1.
RefSeqiNP_067735.1. NM_021703.1.
UniGeneiRn.42933.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009123.

Proteomic databases

PaxDbiO35817.
PRIDEiO35817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009123; ENSRNOP00000009123; ENSRNOG00000006899.
GeneIDi60332.
KEGGirno:60332.
UCSCiRGD:708510. rat.

Organism-specific databases

CTDi158798.
RGDi708510. Akap14.

Phylogenomic databases

eggNOGiENOG410IXR2. Eukaryota.
ENOG4111RMU. LUCA.
GeneTreeiENSGT00390000003444.
HOGENOMiHOG000033875.
HOVERGENiHBG080826.
InParanoidiO35817.
KOiK16530.
OMAiIRFREQW.
OrthoDBiEOG73FQNR.
TreeFamiTF329057.

Miscellaneous databases

NextBioi611969.
PROiO35817.

Gene expression databases

GenevisibleiO35817. RN.

Family and domain databases

InterProiIPR025663. AKAP_28.
[Graphical view]
PfamiPF14469. AKAP28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of a testis-specific, developmentally regulated A-kinase anchoring protein (TAKAP-80) present on the fibrous sheath of rat sperm."
    Mei X., Singh I.S., Erlichman J., Orr G.A.
    Eur. J. Biochem. 246:425-432(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Testis.

Entry informationi

Entry nameiAKA14_RAT
AccessioniPrimary (citable) accession number: O35817
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.