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Protein

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

Gene

Pigl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the second step of GPI biosynthesis. De-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol.1 Publication

Catalytic activityi

6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate.

Pathwayi: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

  • N-acetylglucosaminylphosphatidylinositol deacetylase activity Source: RGD

GO - Biological processi

  • GPI anchor biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

BRENDAi3.5.1.89. 5301.
ReactomeiR-RNO-162710. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (EC:3.5.1.89)
Alternative name(s):
Phosphatidylinositol-glycan biosynthesis class L protein
Short name:
PIG-L
Gene namesi
Name:Pigl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi620437. Pigl.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221HelicalSequence analysisAdd
BLAST
Topological domaini23 – 252230CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extrinsic component of endoplasmic reticulum membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252N-acetylglucosaminyl-phosphatidylinositol de-N-acetylasePRO_0000207168Add
BLAST

Proteomic databases

PaxDbiO35790.

Expressioni

Gene expression databases

GenevisibleiO35790. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004113.

Structurei

3D structure databases

ProteinModelPortaliO35790.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGL family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3332. Eukaryota.
COG2120. LUCA.
GeneTreeiENSGT00390000018434.
HOGENOMiHOG000176611.
HOVERGENiHBG019149.
InParanoidiO35790.
KOiK03434.
OMAiHSNHIAL.
OrthoDBiEOG7KWSJF.
PhylomeDBiO35790.
TreeFamiTF315150.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.

Sequencei

Sequence statusi: Complete.

O35790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVVGLLCVA VAVLTWGFLR VWNSAERMRS PEQAGLPGAG SRALVVIAHP
60 70 80 90 100
DDEAMFFAPT ILGLARLKQQ VSLLCFSSGN YYNQGEIRKK ELLQSCAVLG
110 120 130 140 150
IPPSRVMIID KREFPDDPEV QWDTEHVAST ILQHIHANAT DLVVTFDAEG
160 170 180 190 200
VSGHSNHIAL YKAVRALHSG GKLPEGCSVL TLQSVNVLRK YVFLLDLPWT
210 220 230 240 250
LLSPQGVLFV LTSKEVAQAK KAMSCHRSQL LWFRHLYTVF SRYMSVNSLQ

LL
Length:252
Mass (Da):27,984
Last modified:January 1, 1998 - v1
Checksum:i73AD9AA7A79DBFCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88364 mRNA. Translation: BAA20869.1.
BC074020 mRNA. Translation: AAH74020.1.
RefSeqiNP_620256.1. NM_138901.2.
UniGeneiRn.10774.

Genome annotation databases

EnsembliENSRNOT00000004113; ENSRNOP00000004113; ENSRNOG00000003070.
GeneIDi192263.
KEGGirno:192263.
UCSCiRGD:620437. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88364 mRNA. Translation: BAA20869.1.
BC074020 mRNA. Translation: AAH74020.1.
RefSeqiNP_620256.1. NM_138901.2.
UniGeneiRn.10774.

3D structure databases

ProteinModelPortaliO35790.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004113.

Proteomic databases

PaxDbiO35790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004113; ENSRNOP00000004113; ENSRNOG00000003070.
GeneIDi192263.
KEGGirno:192263.
UCSCiRGD:620437. rat.

Organism-specific databases

CTDi9487.
RGDi620437. Pigl.

Phylogenomic databases

eggNOGiKOG3332. Eukaryota.
COG2120. LUCA.
GeneTreeiENSGT00390000018434.
HOGENOMiHOG000176611.
HOVERGENiHBG019149.
InParanoidiO35790.
KOiK03434.
OMAiHSNHIAL.
OrthoDBiEOG7KWSJF.
PhylomeDBiO35790.
TreeFamiTF315150.

Enzyme and pathway databases

UniPathwayiUPA00196.
BRENDAi3.5.1.89. 5301.
ReactomeiR-RNO-162710. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

PROiO35790.

Gene expression databases

GenevisibleiO35790. RN.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylase."
    Nakamura N., Inoue N., Watanabe R., Takahashi M., Takeda J., Stevens V.L., Kinoshita T.
    J. Biol. Chem. 272:15834-15840(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Glial tumor.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis."
    Watanabe R., Ohishi K., Maeda Y., Nakamura N., Kinoshita T.
    Biochem. J. 339:185-192(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPIGL_RAT
AccessioniPrimary (citable) accession number: O35790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.