Reviewed, UniProtKB/Swiss-Prot O35789 (B3GA1_RAT)

Names and origin

Protein names

Recommended name:
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
    EC=2.4.1.135
Alternative name(s):
    Beta-1,3-glucuronyltransferase 1
    Glucuronosyltransferase-P
      Short name=GlcAT-P
    UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
      Short name=GlcUAT-P

Gene names

Name:

B3gat1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer Potential.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Brain. Greater expression found in the cerebral cortex than the cerebellum.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	Golgi lumen Ref.2 Traceable author statement. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Ref.1 Inferred from direct assay. Source: RGD manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1		PRO_0000195170
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 334	307	Lumenal Potential
Sites
	Active site	284	1	Proton acceptor By similarity
	Metal binding	197	1	Manganese By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O35789-1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 2C9AD8A19AA11379	FASTA	334	38,237
	10 20 30 40 50 60 MPKRRDILAI VLIVLPWTLL ITVWHQSSLA PLLAVHKDEG SDPRHEAPPG ADPREYCMSD 70 80 90 100 110 120 RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA ELTRMANTLL HVPNLHWLVV 130 140 150 160 170 180 EDAPRRTPLT ARLLRDTGLN YTHLHVETPR NYKLRGDARD PRIPRGTMQR NLALRWLRET 190 200 210 220 230 240 FPRNSTQPGV VYFADDDNTY SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG 250 260 270 280 290 300 WKTVFDPHRP FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK 310 320 330 AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI

References

[1]	"Cloning and functional expression of a novel glucuronyltransferase involved in the biosynthesis of the carbohydrate epitope HNK-1." Terayama K., Oka S., Seiki T., Miki Y., Nakamura A., Kozutsumi Y., Takio K., Kawasaki T. Proc. Natl. Acad. Sci. U.S.A. 94:6093-6098(1997) [PubMed: 9177175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 62-95; 185-209; 214-231; 238-262; 269-274; 280-289 AND 307-333. Tissue: Brain.
[2]	"Purification and characterization of a glucuronyltransferase involved in the biosynthesis of the HNK-1 epitope on glycoproteins from rat brain." Terayama K., Seiki T., Nakamura A., Matsumori K., Ohta S., Oka S., Sugita M., Kawasaki T. J. Biol. Chem. 273:30295-30300(1998) [PubMed: 9804790] [Abstract] Cited for: CHARACTERIZATION. Tissue: Fetal brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D88035 mRNA. Translation: BAA20551.1. Different initiation.
IPI	IPI00231088.
RefSeq	NP_446455.1.
UniGene	Rn.88274
3D structure databases
HSSP	HSSP built from PDB template 1KWS based on UniProtKB O94766.
SMR	O35789. Positions 83-334.
ModBase	Search...
Protein-protein interaction databases
STRING	O35789.
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Genome annotation databases
Ensembl	ENSRNOT00000009825; ENSRNOP00000009825; ENSRNOG00000007142; Rattus norvegicus. [Genome view] ENSRNOT00000009912; ENSRNOP00000009912; ENSRNOG00000007142; Rattus norvegicus. [Genome view]
GeneID	117108.
KEGG	rno:117108.
UCSC	D88035. rat.
Organism-specific databases
CTD	117108.
RGD	70880. B3gat1.
Phylogenomic databases
HOVERGEN	O35789.
Enzyme and pathway databases
BRENDA	2.4.1.135. 248.
Gene expression databases
ArrayExpress	O35789.
Genevestigator	O35789.
GermOnline	ENSRNOG00000007142. Rattus norvegicus.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620012.

Entry information

Entry name

B3GA1_RAT

Accession

Primary (citable) accession number: O35789

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	October 13, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)