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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Gene

B3gat1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.2 Publications

Catalytic activityi

UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.2 Publications

Cofactori

Mn2+1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122UDP-glucuronateBy similarity1
Binding sitei165UDP-glucuronateBy similarity1
Binding sitei170UDP-glucuronateBy similarity1
Metal bindingi197ManganeseBy similarity1
Active sitei284Proton donor/acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi91 – 93UDP-glucuronate bindingBy similarity3
Nucleotide bindingi195 – 197UDP-glucuronate bindingBy similarity3
Nucleotide bindingi311 – 313UDP-glucuronate bindingBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1By similarity (EC:2.4.1.1351 Publication)
Alternative name(s):
Beta-1,3-glucuronyltransferase 1
Glucuronosyltransferase P
Short name:
GlcAT-P1 Publication
UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
Short name:
GlcUAT-P
Gene namesi
Name:B3gat1Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi70880. B3gat1.

Subcellular locationi

Isoform 1 :
  • Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein
  • Secreted 1 Publication
Isoform 2 :
  • Golgi apparatus membrane 1 Publication; Single-pass type II membrane protein
  • Endoplasmic reticulum membrane 1 Publication
  • Secreted 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 334LumenalSequence analysisAdd BLAST307

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • extracellular region Source: UniProtKB-SubCell
  • Golgi lumen Source: RGD
  • Golgi membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi3 – 5KRR → AAA: Significantly disrupts Golgi apparatus localization. Decreases galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity. Impairs secretion and interaction with SAR1A. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951701 – 334Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103PhosphothreonineCombined sources1
Modified residuei108PhosphothreonineCombined sources1
Glycosylationi140N-linked (GlcNAc...)Sequence analysis1
Glycosylationi184N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO35789.
PRIDEiO35789.

PTM databases

iPTMnetiO35789.
PhosphoSitePlusiO35789.

Expressioni

Tissue specificityi

Brain. Greater expression found in the cerebral cortex than the cerebellum.

Gene expression databases

BgeeiENSRNOG00000007142.
GenevisibleiO35789. RN.

Interactioni

Subunit structurei

Homodimer (PubMed:9804790). Interacts with SAR1A (PubMed:19181664).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei228Interaction with galactose moiety of substrate glycoproteinBy similarity1
Sitei321Interaction with galactose moiety of substrate glycoproteinBy similarity1

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009825.

Structurei

3D structure databases

ProteinModelPortaliO35789.
SMRiO35789.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni3 – 5Essential for transport from endoplasmic reticulum to Golgi apparatus and interaction with SAR1A1 Publication3
Regioni245 – 254Interaction with galactose moiety of substrate glycoproteinBy similarity10

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiO35789.
KOiK00735.
OrthoDBiEOG091G0G8P.
TreeFamiTF313522.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35789-1) [UniParc]FASTAAdd to basket
Also known as: sGlcAT-P1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKRRDILAI VLIVLPWTLL ITVWHQSSLA PLLAVHKDEG SDPRHEAPPG
60 70 80 90 100
ADPREYCMSD RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA
110 120 130 140 150
ELTRMANTLL HVPNLHWLVV EDAPRRTPLT ARLLRDTGLN YTHLHVETPR
160 170 180 190 200
NYKLRGDARD PRIPRGTMQR NLALRWLRET FPRNSTQPGV VYFADDDNTY
210 220 230 240 250
SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG WKTVFDPHRP
260 270 280 290 300
FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK
310 320 330
AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI
Length:334
Mass (Da):38,237
Last modified:June 1, 2001 - v2
Checksum:i2C9AD8A19AA11379
GO
Isoform 2 (identifier: O35789-2) [UniParc]FASTAAdd to basket
Also known as: lGlcAT-P1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGNEELWAQPALEM

Show »
Length:347
Mass (Da):39,707
Checksum:iFA873D28A9CE655D
GO

Sequence cautioni

The sequence BAA20551 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0585401M → MGNEELWAQPALEM in isoform 2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88035 mRNA. Translation: BAA20551.1. Different initiation.
AABR07069533 Genomic DNA. No translation available.
CH474007 Genomic DNA. Translation: EDL83354.1.
CH474007 Genomic DNA. Translation: EDL83353.1.
CH474007 Genomic DNA. Translation: EDL83351.1.
RefSeqiNP_446455.1. NM_054003.1. [O35789-2]
XP_006242795.1. XM_006242733.3. [O35789-2]
XP_006242796.1. XM_006242734.3. [O35789-1]
UniGeneiRn.88274.

Genome annotation databases

EnsembliENSRNOT00000009825; ENSRNOP00000009825; ENSRNOG00000007142. [O35789-2]
GeneIDi117108.
KEGGirno:117108.
UCSCiRGD:70880. rat. [O35789-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88035 mRNA. Translation: BAA20551.1. Different initiation.
AABR07069533 Genomic DNA. No translation available.
CH474007 Genomic DNA. Translation: EDL83354.1.
CH474007 Genomic DNA. Translation: EDL83353.1.
CH474007 Genomic DNA. Translation: EDL83351.1.
RefSeqiNP_446455.1. NM_054003.1. [O35789-2]
XP_006242795.1. XM_006242733.3. [O35789-2]
XP_006242796.1. XM_006242734.3. [O35789-1]
UniGeneiRn.88274.

3D structure databases

ProteinModelPortaliO35789.
SMRiO35789.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009825.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

PTM databases

iPTMnetiO35789.
PhosphoSitePlusiO35789.

Proteomic databases

PaxDbiO35789.
PRIDEiO35789.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009825; ENSRNOP00000009825; ENSRNOG00000007142. [O35789-2]
GeneIDi117108.
KEGGirno:117108.
UCSCiRGD:70880. rat. [O35789-1]

Organism-specific databases

CTDi27087.
RGDi70880. B3gat1.

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiO35789.
KOiK00735.
OrthoDBiEOG091G0G8P.
TreeFamiTF313522.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-RNO-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

PROiO35789.

Gene expression databases

BgeeiENSRNOG00000007142.
GenevisibleiO35789. RN.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB3GA1_RAT
AccessioniPrimary (citable) accession number: O35789
Secondary accession number(s): A0A0H2UHF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.