Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Gene

B3gat1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Catalytic activityi

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei122 – 1221UDP-glucuronateBy similarity
Binding sitei165 – 1651UDP-glucuronateBy similarity
Binding sitei170 – 1701UDP-glucuronateBy similarity
Metal bindingi197 – 1971ManganeseBy similarity
Active sitei284 – 2841Proton donor/acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi91 – 933UDP-glucuronate bindingBy similarity
Nucleotide bindingi195 – 1973UDP-glucuronate bindingBy similarity
Nucleotide bindingi311 – 3133UDP-glucuronate bindingBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (EC:2.4.1.135)
Alternative name(s):
Beta-1,3-glucuronyltransferase 1
Glucuronosyltransferase P
Short name:
GlcAT-P
UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
Short name:
GlcUAT-P
Gene namesi
Name:B3gat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70880. B3gat1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 334307LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • Golgi lumen Source: RGD
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1PRO_0000195170Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031PhosphothreonineCombined sources
Modified residuei108 – 1081PhosphothreonineCombined sources
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO35789.

PTM databases

iPTMnetiO35789.
PhosphoSiteiO35789.

Expressioni

Tissue specificityi

Brain. Greater expression found in the cerebral cortex than the cerebellum.

Gene expression databases

BgeeiENSRNOG00000007142.
GenevisibleiO35789. RN.

Interactioni

Subunit structurei

Homodimer.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei228 – 2281Interaction with galactose moiety of substrate glycoproteinBy similarity
Sitei321 – 3211Interaction with galactose moiety of substrate glycoproteinBy similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009825.

Structurei

3D structure databases

ProteinModelPortaliO35789.
SMRiO35789. Positions 83-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni245 – 25410Interaction with galactose moiety of substrate glycoproteinBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiO35789.
KOiK00735.
OrthoDBiEOG091G0G8P.
TreeFamiTF313522.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

O35789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRRDILAI VLIVLPWTLL ITVWHQSSLA PLLAVHKDEG SDPRHEAPPG
60 70 80 90 100
ADPREYCMSD RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA
110 120 130 140 150
ELTRMANTLL HVPNLHWLVV EDAPRRTPLT ARLLRDTGLN YTHLHVETPR
160 170 180 190 200
NYKLRGDARD PRIPRGTMQR NLALRWLRET FPRNSTQPGV VYFADDDNTY
210 220 230 240 250
SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG WKTVFDPHRP
260 270 280 290 300
FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK
310 320 330
AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI
Length:334
Mass (Da):38,237
Last modified:June 1, 2001 - v2
Checksum:i2C9AD8A19AA11379
GO

Sequence cautioni

The sequence BAA20551 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88035 mRNA. Translation: BAA20551.1. Different initiation.
RefSeqiNP_446455.1. NM_054003.1.
XP_006242795.1. XM_006242733.2.
XP_006242796.1. XM_006242734.2.
UniGeneiRn.88274.

Genome annotation databases

GeneIDi117108.
KEGGirno:117108.
UCSCiRGD:70880. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88035 mRNA. Translation: BAA20551.1. Different initiation.
RefSeqiNP_446455.1. NM_054003.1.
XP_006242795.1. XM_006242733.2.
XP_006242796.1. XM_006242734.2.
UniGeneiRn.88274.

3D structure databases

ProteinModelPortaliO35789.
SMRiO35789. Positions 83-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009825.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

PTM databases

iPTMnetiO35789.
PhosphoSiteiO35789.

Proteomic databases

PaxDbiO35789.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117108.
KEGGirno:117108.
UCSCiRGD:70880. rat.

Organism-specific databases

CTDi27087.
RGDi70880. B3gat1.

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiO35789.
KOiK00735.
OrthoDBiEOG091G0G8P.
TreeFamiTF313522.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiO35789.

Gene expression databases

BgeeiENSRNOG00000007142.
GenevisibleiO35789. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB3GA1_RAT
AccessioniPrimary (citable) accession number: O35789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.