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Protein

Class E basic helix-loop-helix protein 41

Gene

Bhlhb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • circadian rhythm Source: UniProtKB
  • entrainment of circadian clock Source: RGD
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • nervous system development Source: RGD
  • regulation of neuronal synaptic plasticity Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class E basic helix-loop-helix protein 41
Short name:
bHLHe41
Alternative name(s):
Class B basic helix-loop-helix protein 3
Short name:
bHLHb3
Enhancer-of-split and hairy-related protein 1
Short name:
SHARP-1
Gene namesi
Name:Bhlhb3
Synonyms:Sharp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi70900. Bhlhb3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271491 – 410Class E basic helix-loop-helix protein 41Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki240Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiO35779.

PTM databases

PhosphoSitePlusiO35779.

Expressioni

Tissue specificityi

Highly expressed in the caudate putamen, pineal gland, granular cell layer of the cerebellum, olfactory bulb, piriform cortex, hippocampus and hypothalamic nuclei. Moderately expressed in skeletal muscle, heart. Weakly expressed in lung.1 Publication

Inductioni

Expressed in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain.1 Publication

Interactioni

Subunit structurei

Homodimer. Heterodimer with BHLHE40/DEC1. Interacts with CIART. Interacts with ARNTL/BMAL1 and RXRA (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO35779.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 99bHLHPROSITE-ProRule annotationAdd BLAST56
Domaini131 – 166OrangePROSITE-ProRule annotationAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi321 – 344Ala/Gly-richAdd BLAST24

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiO35779.
PhylomeDBiO35779.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35779-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEGIPHLQE RQLLEHRDFI GLDYSSLYMC KPKRSLKRDD TKDTYKLPHR
60 70 80 90 100
LIEKKRRDRI NECIAQLKDL LPEHLKLTTL GHLEKAVVLE LTLKHLKALT
110 120 130 140 150
ALTEQQHQKI IALQNGERSL KSPVQADLDA FHSGFQTCAK EVLQYLARFE
160 170 180 190 200
SWTPREPRCA QLVSHLHAVA TQLLTPQVTP GRGPGRAPCS AGAAAASGSE
210 220 230 240 250
RVARCVPVIQ RTQPGTEPEH DTDTDSGYGG EAEQGRAAVK QEPPGDPSAA
260 270 280 290 300
PKRLKLEARG ALLGPEPALL GSLVALGGGA PFAQPAAAPF CLPFYLLSPS
310 320 330 340 350
AAAYVQPWLD KSGLDKYLYP AAAAPFPLLY PGIPAAAAAA AAAAFPCLSS
360 370 380 390 400
VLSPPPEKAG SAAGAPFLAH EVAPPGSLRP QHAHSRTHLP HAVNPESSQE
410
DATQPAKDAP
Length:410
Mass (Da):43,917
Last modified:March 5, 2002 - v2
Checksum:i829705CA3A013127
GO

Sequence cautioni

The sequence AAB63586 differs from that shown. Reason: Frameshift at position 249.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009329 mRNA. Translation: AAB63586.1. Frameshift.
UniGeneiRn.10784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009329 mRNA. Translation: AAB63586.1. Frameshift.
UniGeneiRn.10784.

3D structure databases

ProteinModelPortaliO35779.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSitePlusiO35779.

Proteomic databases

PRIDEiO35779.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi70900. Bhlhb3.

Phylogenomic databases

InParanoidiO35779.
PhylomeDBiO35779.

Miscellaneous databases

PROiO35779.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBHE41_RAT
AccessioniPrimary (citable) accession number: O35779
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 5, 2002
Last modified: November 2, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.