Reviewed, UniProtKB/Swiss-Prot O35776 (HAS2_RAT)

Names and origin

Protein names

Recommended name:
    Hyaluronan synthase 2
    EC=2.4.1.212
Alternative name(s):
    Hyaluronate synthase 2
    Hyaluronic acid synthase 2
      Short name=HA synthase 2

Gene names

Name:

Has2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a role in hyaluronan/hyaluronic acid (HA) synthesis By similarity.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the nodC/HAS family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Hyaluronan synthase 2		PRO_0000197175
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	1 Potential
	Topological domain	33 – 45	13	Extracellular Potential
	Transmembrane	46 – 66	21	2 Potential
	Topological domain	67 – 374	308	Cytoplasmic Potential
	Transmembrane	375 – 395	21	3 Potential
	Topological domain	396 – 402	7	Extracellular Potential
	Transmembrane	403 – 423	21	4 Potential
	Topological domain	424 – 429	6	Cytoplasmic Potential
	Transmembrane	430 – 450	21	5 Potential
	Topological domain	451 – 475	25	Extracellular Potential
	Transmembrane	476 – 496	21	6 Potential
	Topological domain	497 – 510	14	Cytoplasmic Potential
	Transmembrane	511 – 531	21	7 Potential
	Topological domain	532 – 552	21	Extracellular Potential

Sequences

	Sequence		Length	Mass (Da)
	O35776-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 1DCAA244E3A4E9BC	FASTA	552	63,534
	10 20 30 40 50 60 MHCERFLCVL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG AFLASHLIIQ 70 80 90 100 110 120 SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR KCLQSVKRLT YPGIKVVMVI 130 140 150 160 170 180 DGNSDDDLYM MDIFSEVMGR DKSVTYIWKN NFHERGPGET EESHKESSQH VTQLVLSNKS 190 200 210 220 230 240 ICIMQKWGGK REVMYTAFRA LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG 250 260 270 280 290 300 DVQILNKYDS WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY 310 320 330 340 350 360 NQEFMGNQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ QTRWSKSYFR 370 380 390 400 410 420 EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR GKIWNILLFL LTVQLVGLIK 430 440 450 460 470 480 SSFASCLRGN IVMVFMSLYS VLYMSSLLPA KMFAIATINK AGWGTSGRKT IVVNFIGLIP 490 500 510 520 530 540 VSVWFTILLG GVIFTIYKES KKPFSESKQT VLIVGTLIYA CYWVVLLTLY VVLINKCGRR 550 KKGQQYDMVL DV

References

[1]	"Molecular cloning of rat hyaluronan synthase 2 and regulation of its expression in a rat osteoblastic cell line." Sakaguchi K., Midura R.J. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF008201 mRNA. Translation: AAB63209.1.
IPI	IPI00212371.
RefSeq	NP_037285.1.
UniGene	Rn.87393
3D structure databases
SMR	O35776. Positions 84-321.
ModBase	Search...
Protein-protein interaction databases
STRING	O35776.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
Ensembl	ENSRNOT00000006517; ENSRNOP00000006517; ENSRNOG00000004854; Rattus norvegicus. [Genome view]
GeneID	25694.
KEGG	rno:25694.
UCSC	NM_013153. rat.
Organism-specific databases
CTD	25694.
RGD	2781. Has2.
Phylogenomic databases
eggNOG	maNOG07444.
HOVERGEN	O35776.
InParanoid	O35776.
OMA	CERFICI.
OrthoDB	EOG947JD5.
PhylomeDB	O35776.
Enzyme and pathway databases
BRENDA	2.4.1.212. 248.
Gene expression databases
Genevestigator	O35776.
GermOnline	ENSRNOG00000004854. Rattus norvegicus.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	607703.

Entry information

Entry name

HAS2_RAT

Accession

Primary (citable) accession number: O35776

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	January 19, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)