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Protein

Hyaluronan synthase 2

Gene

Has2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix (By similarity).By similarity

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).

Cofactori

Pathwayi: hyaluronan biosynthesis

This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • hyaluronan synthase activity Source: UniProtKB

GO - Biological processi

  • atrioventricular canal development Source: UniProtKB
  • bone morphogenesis Source: Ensembl
  • cellular response to fluid shear stress Source: Ensembl
  • cellular response to interleukin-1 Source: Ensembl
  • cellular response to platelet-derived growth factor stimulus Source: Ensembl
  • cellular response to tumor necrosis factor Source: Ensembl
  • endocardial cushion to mesenchymal transition Source: UniProtKB
  • estrous cycle Source: RGD
  • extracellular matrix assembly Source: UniProtKB
  • extracellular polysaccharide biosynthetic process Source: UniProtKB
  • hyaluronan biosynthetic process Source: UniProtKB
  • kidney development Source: UniProtKB
  • positive regulation of hyaluronan biosynthetic process Source: RGD
  • positive regulation of keratinocyte migration Source: RGD
  • positive regulation of keratinocyte proliferation Source: RGD
  • positive regulation of monocyte aggregation Source: Ensembl
  • positive regulation of smooth muscle cell migration Source: RGD
  • positive regulation of substrate adhesion-dependent cell spreading Source: RGD
  • positive regulation of urine volume Source: UniProtKB
  • renal water absorption Source: UniProtKB
  • vasculogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.212. 5301.
UniPathwayiUPA00341.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronan synthase 2 (EC:2.4.1.212)
Alternative name(s):
Hyaluronate synthase 2
Hyaluronic acid synthase 2
Short name:
HA synthase 2
Gene namesi
Name:Has2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi2781. Has2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini33 – 4513ExtracellularSequence analysisAdd
BLAST
Transmembranei46 – 6621Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini67 – 374308CytoplasmicSequence analysisAdd
BLAST
Transmembranei375 – 39521Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini396 – 4027ExtracellularSequence analysis
Transmembranei403 – 42321Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini424 – 4296CytoplasmicSequence analysis
Transmembranei430 – 45021Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini451 – 47525ExtracellularSequence analysisAdd
BLAST
Transmembranei476 – 49621Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini497 – 51014CytoplasmicSequence analysisAdd
BLAST
Transmembranei511 – 53121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini532 – 55221ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 552552Hyaluronan synthase 2PRO_0000197175Add
BLAST

Proteomic databases

PaxDbiO35776.
PRIDEiO35776.

Expressioni

Gene expression databases

GenevisibleiO35776. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006517.

Structurei

3D structure databases

ProteinModelPortaliO35776.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NodC/HAS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2571. Eukaryota.
COG1215. LUCA.
GeneTreeiENSGT00390000010337.
HOGENOMiHOG000112847.
HOVERGENiHBG000189.
InParanoidiO35776.
KOiK00752.
OMAiTAYIMGY.
OrthoDBiEOG77WWBZ.
PhylomeDBiO35776.
TreeFamiTF332506.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR026107. HAS/NodC.
IPR028371. HAS2.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF7. PTHR22913:SF7. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

O35776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHCERFLCVL RIIGTTLFGV SLLLGITAAY IVGYQFIQTD NYYFSFGLYG
60 70 80 90 100
AFLASHLIIQ SLFAFLEHRK MKKSLETPIK LNKTVALCIA AYQEDPDYLR
110 120 130 140 150
KCLQSVKRLT YPGIKVVMVI DGNSDDDLYM MDIFSEVMGR DKSVTYIWKN
160 170 180 190 200
NFHERGPGET EESHKESSQH VTQLVLSNKS ICIMQKWGGK REVMYTAFRA
210 220 230 240 250
LGRSVDYVQV CDSDTMLDPA SSVEMVKVLE EDPMVGGVGG DVQILNKYDS
260 270 280 290 300
WISFLSSVRY WMAFNIERAC QSYFGCVQCI SGPLGMYRNS LLHEFVEDWY
310 320 330 340 350
NQEFMGNQCS FGDDRHLTNR VLSLGYATKY TARSKCLTET PIEYLRWLNQ
360 370 380 390 400
QTRWSKSYFR EWLYNAMWFH KHHLWMTYEA VITGFFPFFL IATVIQLFYR
410 420 430 440 450
GKIWNILLFL LTVQLVGLIK SSFASCLRGN IVMVFMSLYS VLYMSSLLPA
460 470 480 490 500
KMFAIATINK AGWGTSGRKT IVVNFIGLIP VSVWFTILLG GVIFTIYKES
510 520 530 540 550
KKPFSESKQT VLIVGTLIYA CYWVVLLTLY VVLINKCGRR KKGQQYDMVL

DV
Length:552
Mass (Da):63,534
Last modified:January 1, 1998 - v1
Checksum:i1DCAA244E3A4E9BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008201 mRNA. Translation: AAB63209.1.
RefSeqiNP_037285.1. NM_013153.1.
UniGeneiRn.87393.

Genome annotation databases

EnsembliENSRNOT00000006517; ENSRNOP00000006517; ENSRNOG00000004854.
GeneIDi25694.
KEGGirno:25694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008201 mRNA. Translation: AAB63209.1.
RefSeqiNP_037285.1. NM_013153.1.
UniGeneiRn.87393.

3D structure databases

ProteinModelPortaliO35776.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006517.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Proteomic databases

PaxDbiO35776.
PRIDEiO35776.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000006517; ENSRNOP00000006517; ENSRNOG00000004854.
GeneIDi25694.
KEGGirno:25694.

Organism-specific databases

CTDi3037.
RGDi2781. Has2.

Phylogenomic databases

eggNOGiKOG2571. Eukaryota.
COG1215. LUCA.
GeneTreeiENSGT00390000010337.
HOGENOMiHOG000112847.
HOVERGENiHBG000189.
InParanoidiO35776.
KOiK00752.
OMAiTAYIMGY.
OrthoDBiEOG77WWBZ.
PhylomeDBiO35776.
TreeFamiTF332506.

Enzyme and pathway databases

UniPathwayiUPA00341.
BRENDAi2.4.1.212. 5301.

Miscellaneous databases

PROiO35776.

Gene expression databases

GenevisibleiO35776. RN.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR026107. HAS/NodC.
IPR028371. HAS2.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF7. PTHR22913:SF7. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of rat hyaluronan synthase 2 and regulation of its expression in a rat osteoblastic cell line."
    Sakaguchi K., Midura R.J.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHYAS2_RAT
AccessioniPrimary (citable) accession number: O35776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.