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Protein

A-kinase anchor protein 4

Gene

Akap4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Major structural component of sperm fibrous sheath. May play a role in sperm motility (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 4
Short name:
AKAP-4
Alternative name(s):
75 kDa fibrous sheath protein
Major sperm fibrous sheath protein
Protein kinase A-anchoring protein 4
Short name:
PRKA4
Gene namesi
Name:Akap4Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620828. Akap4.

Subcellular locationi

  • Cell projectionciliumflagellum By similarity

  • Note: Localizes to the principle piece of the sperm flagellum.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Flagellum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 187187Sequence analysisPRO_0000248232Add
BLAST
Chaini188 – 847660A-kinase anchor protein 4By similarityPRO_0000248233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei95 – 951PhosphoserineCombined sources
Modified residuei129 – 1291PhosphoserineCombined sources
Modified residuei189 – 1891PhosphoserineCombined sources
Modified residuei203 – 2031PhosphoserineCombined sources
Modified residuei206 – 2061PhosphothreonineCombined sources
Modified residuei212 – 2121PhosphoserineCombined sources
Modified residuei225 – 2251PhosphoserineCombined sources
Modified residuei270 – 2701PhosphoserineCombined sources
Modified residuei300 – 3001PhosphotyrosineBy similarity
Modified residuei301 – 3011PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei340 – 3401PhosphoserineCombined sources
Modified residuei430 – 4301PhosphoserineCombined sources
Modified residuei441 – 4411PhosphoserineCombined sources
Modified residuei443 – 4431PhosphoserineCombined sources
Modified residuei462 – 4621PhosphoserineCombined sources
Modified residuei491 – 4911PhosphoserineCombined sources
Modified residuei496 – 4961PhosphoserineCombined sources
Modified residuei503 – 5031PhosphoserineCombined sources
Modified residuei505 – 5051PhosphothreonineCombined sources
Modified residuei536 – 5361PhosphoserineCombined sources
Modified residuei626 – 6261PhosphoserineCombined sources
Modified residuei631 – 6311PhosphoserineCombined sources
Modified residuei648 – 6481PhosphoserineCombined sources
Modified residuei650 – 6501PhosphoserineCombined sources
Modified residuei674 – 6741PhosphoserineCombined sources
Modified residuei677 – 6771PhosphoserineCombined sources
Modified residuei700 – 7001PhosphoserineCombined sources
Modified residuei729 – 7291PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO35774.
PRIDEiO35774.

PTM databases

iPTMnetiO35774.

Expressioni

Tissue specificityi

Expressed in flagella of epididymal sperm.1 Publication

Interactioni

Subunit structurei

Interacts with PRKAR1A and PRKAR2A. Interacts with ENO4.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003939.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 23114Interaction with Prkar1a and Prkar2aBy similarityAdd
BLAST
Regioni334 – 34310PKA-RI subunit binding domainBy similarity

Domaini

RI-alpha binding site, predicted to form an amphipathic helix that is required for binding to Prkar1a.By similarity

Sequence similaritiesi

Belongs to the AKAP110 family.Curated

Phylogenomic databases

HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiO35774.
KOiK16521.
PhylomeDBiO35774.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
[Graphical view]
PfamiPF05716. AKAP_110. 3 hits.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAYCGTTKM SDDIDWLHSR RGVCKVDLYS PEGQQDQDRK VICFVDVSTL
60 70 80 90 100
NVEDDSKGAA GPRSDGELNL ENLEEKEIIV IKDTEKQDQP KTEGSVCLFK
110 120 130 140 150
QAPSDPISVL NWLLNDLQKY ALGFQHALSP SASSCKHKVG DLEGDYHKIP
160 170 180 190 200
SENCYSVYAD QVNLDYLNKG PQNLRLEMAA SKNTNNNQSP SNPATKSPSN
210 220 230 240 250
QRSVATPDGE CSMDDLSYYV NRLSSLVIQM ARKEIKDKLE GGNKCLHHSM
260 270 280 290 300
YTSGEKGKTS PRSAVSKIAS EMAHEAVELT SSEMRGNGEE GRDGRKTFLY
310 320 330 340 350
SELSNKNKCG EKQQMCPKDS KEFADSISKG LMVYANQVAS DMMVSVMKTL
360 370 380 390 400
KVHSCGKPIP ACVVLKRVLL KHTKEIVSDL IDSCMKNLHN ITGVLMTDSD
410 420 430 440 450
FVSAVKRNLF NHGKQNAADI MEAMLKRLVS ALLGEKKETK SQSLAYATLK
460 470 480 490 500
AGTHDPKCKN QSLEFSAMKA EMKGKDKGKT KGDPCCKSLT SAERVSEHIL
510 520 530 540 550
KESLTMWNNQ KQGTQGRVPN KVCPSKDEKR EKISPSTDSL AKDLIVSALM
560 570 580 590 600
LIQYHLTQQA KGKDPCEEEC PGSSMGYMSQ SAQYEKSGGG QSSKSLSMKH
610 620 630 640 650
FESRGAPGPS TCAKENQLES QKMDMSNMVL SLIQKLLSES PFSCDELSES
660 670 680 690 700
ENKRCCDSRS KQAAPVAKRP EDQSQDSTEM DFISGMKQMN RQFIDQLVES
710 720 730 740 750
VMKLCLIMAK YSNNGAALAE LEEQAALASN GPRCGREAVM SQSYLETPGP
760 770 780 790 800
EVIVNNQCST SNLQKQLQAV LQWIAASQFN VPMLYFMGDD DGQLEKLPEV
810 820 830 840
SAKAAEKGYS VGDLLQEVMK FAKERQLDEA VGNMARKQLL DWLLANL
Length:847
Mass (Da):93,494
Last modified:January 1, 1998 - v1
Checksum:iA8F3DB78EA2C3914
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008114 mRNA. Translation: AAB62877.1.
RefSeqiNP_077378.1. NM_024402.1.
UniGeneiRn.10777.

Genome annotation databases

GeneIDi79254.
KEGGirno:79254.
UCSCiRGD:620828. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008114 mRNA. Translation: AAB62877.1.
RefSeqiNP_077378.1. NM_024402.1.
UniGeneiRn.10777.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003939.

PTM databases

iPTMnetiO35774.

Proteomic databases

PaxDbiO35774.
PRIDEiO35774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi79254.
KEGGirno:79254.
UCSCiRGD:620828. rat.

Organism-specific databases

CTDi8852.
RGDi620828. Akap4.

Phylogenomic databases

HOGENOMiHOG000220883.
HOVERGENiHBG050478.
InParanoidiO35774.
KOiK16521.
PhylomeDBiO35774.

Miscellaneous databases

PROiO35774.

Family and domain databases

InterProiIPR020799. AKAP_110.
IPR018292. AKAP_110_C.
IPR018459. RII_binding_1.
[Graphical view]
PfamiPF05716. AKAP_110. 3 hits.
PF10522. RII_binding_1. 1 hit.
[Graphical view]
SMARTiSM00807. AKAP_110. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and developmental expression of the 75 kDa protein of the rat fibrous sheath."
    El-Alfy M., Moshonas D., Morales C., Oko R.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-DawleyImported.
  2. "Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
    Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
    Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-95; SER-129; SER-189; SER-203; THR-206; SER-212; SER-225; SER-270; SER-301; SER-304; SER-340; SER-430; SER-441; SER-443; SER-462; SER-491; SER-496; SER-503; THR-505; SER-536; SER-626; SER-631; SER-648; SER-650; SER-674; SER-677; SER-700 AND SER-729, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiAKAP4_RAT
AccessioniPrimary (citable) accession number: O35774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.