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Protein

Homeobox protein Nkx-2.5

Gene

Nkx2-5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi137 – 196HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • histone deacetylase binding Source: RGD
  • protein homodimerization activity Source: RGD
  • sequence-specific DNA binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

  • cardiac muscle cell differentiation Source: RGD
  • cardiac muscle contraction Source: InterPro
  • cellular response to growth factor stimulus Source: RGD
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • regulation of cardiac conduction Source: InterPro
  • response to estradiol Source: RGD
  • spleen development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-2.5
Short name:
rNKx-2.5
Alternative name(s):
Homeobox protein NK-2 homolog E
Gene namesi
Name:Nkx2-5
Synonyms:Csx, Nkx-2.5, Nkx2e
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620520. Nkx2-5.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000489391 – 318Homeobox protein Nkx-2.5Add BLAST318

Proteomic databases

PaxDbiO35767.
PRIDEiO35767.

PTM databases

PhosphoSitePlusiO35767.

Interactioni

Subunit structurei

Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Interacts with TBX18.By similarity

GO - Molecular functioni

  • histone deacetylase binding Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028155.

Structurei

3D structure databases

ProteinModelPortaliO35767.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi41 – 107Ala/Pro-richAdd BLAST67
Compositional biasi206 – 280Ala/Pro-richAdd BLAST75

Sequence similaritiesi

Belongs to the NK-2 homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
HOGENOMiHOG000231923.
HOVERGENiHBG006689.
InParanoidiO35767.
KOiK09345.
PhylomeDBiO35767.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR033629. NKX-2.5.
[Graphical view]
PANTHERiPTHR24340:SF28. PTHR24340:SF28. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35767-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPSPALTHT PFSVKDILNL EQQQRSLAAG DLSARLEATL APASCMLAAF
60 70 80 90 100
KPDGYSGPEA AAPGLAELRA ELGPAPSPPK CSPAFPTAPT FYPRAYGDPD
110 120 130 140 150
PAKDPRADKK ELCALQKAVE LDKAETDGAE RRRPRRRRKP RVLFSQAQVY
160 170 180 190 200
ELERRFKQQR YLSPAERDQL ASVLKLTSTQ VKIWFQNRRY KCKRQRQDQT
210 220 230 240 250
LELLGPPPPP ARRIAVPVLV RDGKPCLGDS AAYAPAYGLG LNAYGYNAYP
260 270 280 290 300
YPGYGGAACS PAYSCAAYPA APPAAHAPAA SANSNFVNFG VGDLNTVQSP
310
GMPQGNSGVS TLHGIRAW
Length:318
Mass (Da):34,336
Last modified:January 1, 1998 - v1
Checksum:iFCF845D4C41DA87C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006664 mRNA. Translation: AAB62696.1.
RefSeqiNP_446103.1. NM_053651.1.
UniGeneiRn.6179.

Genome annotation databases

GeneIDi114109.
KEGGirno:114109.
UCSCiRGD:620520. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006664 mRNA. Translation: AAB62696.1.
RefSeqiNP_446103.1. NM_053651.1.
UniGeneiRn.6179.

3D structure databases

ProteinModelPortaliO35767.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028155.

PTM databases

PhosphoSitePlusiO35767.

Proteomic databases

PaxDbiO35767.
PRIDEiO35767.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi114109.
KEGGirno:114109.
UCSCiRGD:620520. rat.

Organism-specific databases

CTDi1482.
RGDi620520. Nkx2-5.

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
HOGENOMiHOG000231923.
HOVERGENiHBG006689.
InParanoidiO35767.
KOiK09345.
PhylomeDBiO35767.

Miscellaneous databases

PROiO35767.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR033629. NKX-2.5.
[Graphical view]
PANTHERiPTHR24340:SF28. PTHR24340:SF28. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNKX25_RAT
AccessioniPrimary (citable) accession number: O35767
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.