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Protein

Homeobox protein Nkx-6.1

Gene

Nkx6-1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi237 – 29660HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: RGD
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: RGD
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: RGD

GO - Biological processi

  • cell proliferation Source: RGD
  • cellular response to cytokine stimulus Source: RGD
  • cellular response to peptide hormone stimulus Source: RGD
  • negative regulation of glial cell differentiation Source: Ensembl
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • neurogenesis Source: RGD
  • oligodendrocyte differentiation Source: Ensembl
  • positive regulation of glial cell differentiation Source: Ensembl
  • positive regulation of insulin secretion Source: RGD
  • positive regulation of neuron differentiation Source: Ensembl
  • regulation of axon extension Source: Ensembl
  • regulation of neuron migration Source: Ensembl
  • regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification Source: Ensembl
  • regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification Source: Ensembl
  • response to drug Source: RGD
  • response to nicotine Source: RGD
  • smoothened signaling pathway Source: Ensembl
  • transcription from RNA polymerase II promoter Source: RGD
  • type B pancreatic cell development Source: RGD
  • type B pancreatic cell proliferation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Nkx-6.1
Alternative name(s):
Homeobox protein NK-6 homolog A
Gene namesi
Name:Nkx6-1
Synonyms:Nkx6.1, Nkx6a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi69318. Nkx6-1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Homeobox protein Nkx-6.1PRO_0000048954Add
BLAST

Proteomic databases

PaxDbiO35762.
PRIDEiO35762.

Expressioni

Tissue specificityi

Pancreatic beta cells.

Gene expression databases

GenevisibleiO35762. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002928.

Structurei

3D structure databases

ProteinModelPortaliO35762.
SMRiO35762. Positions 244-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni102 – 269168Repressor domainBy similarityAdd
BLAST
Regioni307 – 36559Involved in DNA-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi49 – 6113Poly-SerAdd
BLAST
Compositional biasi119 – 13214Poly-SerAdd
BLAST
Compositional biasi136 – 15116Poly-AlaAdd
BLAST
Compositional biasi169 – 1746Poly-Pro
Compositional biasi323 – 3264Poly-Asp

Domaini

The C-terminal domain contributes to sequence-specific DNA-binding.

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0847. Eukaryota.
ENOG410XT1X. LUCA.
GeneTreeiENSGT00840000129678.
HOGENOMiHOG000060127.
HOVERGENiHBG070987.
InParanoidiO35762.
KOiK08030.
OMAiHASEAEG.
OrthoDBiEOG7Z95QB.
PhylomeDBiO35762.
TreeFamiTF327063.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
IPR033630. NKX-6.1.
[Graphical view]
PANTHERiPTHR24340:SF31. PTHR24340:SF31. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35762-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAVGAMEGP RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPTGPPSS
60 70 80 90 100
SSSSSSSSSP SPPLGAHNPG GLKPPAAGGL SSLGSPPQQL SAATPHGIND
110 120 130 140 150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASATSASAAA AAAAAAAAAA
160 170 180 190 200
ASSPAGLLAG LPRFSSLSPP PPPPGLYFSP SAAAVAAVGR YPKPLAELPG
210 220 230 240 250
RTPIFWPGVM QSPPWRDARL ACTPHQGSIL LDKDGKRKHT RPTFSGQQIF
260 270 280 290 300
ALEKTFEQTK YLAGPERARL AYSLGMTESQ VKVWFQNRRT KWRKKHAAEM
310 320 330 340 350
ATAKKKQDSE TERLKGTSEN EEDDDDYNKP LDPNSDDEKI TQLLKKHKSS
360
GGSLLLHASE AEGSS
Length:365
Mass (Da):37,689
Last modified:January 1, 1998 - v1
Checksum:iC4AAB702D051F1F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004431 mRNA. Translation: AAB61665.1.
RefSeqiNP_113925.1. NM_031737.1.
UniGeneiRn.11387.

Genome annotation databases

EnsembliENSRNOT00000002928; ENSRNOP00000002928; ENSRNOG00000002149.
GeneIDi65193.
KEGGirno:65193.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF004431 mRNA. Translation: AAB61665.1.
RefSeqiNP_113925.1. NM_031737.1.
UniGeneiRn.11387.

3D structure databases

ProteinModelPortaliO35762.
SMRiO35762. Positions 244-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002928.

Proteomic databases

PaxDbiO35762.
PRIDEiO35762.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002928; ENSRNOP00000002928; ENSRNOG00000002149.
GeneIDi65193.
KEGGirno:65193.

Organism-specific databases

CTDi4825.
RGDi69318. Nkx6-1.

Phylogenomic databases

eggNOGiKOG0847. Eukaryota.
ENOG410XT1X. LUCA.
GeneTreeiENSGT00840000129678.
HOGENOMiHOG000060127.
HOVERGENiHBG070987.
InParanoidiO35762.
KOiK08030.
OMAiHASEAEG.
OrthoDBiEOG7Z95QB.
PhylomeDBiO35762.
TreeFamiTF327063.

Miscellaneous databases

PROiO35762.

Gene expression databases

GenevisibleiO35762. RN.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR000047. HTH_motif.
IPR033630. NKX-6.1.
[Graphical view]
PANTHERiPTHR24340:SF31. PTHR24340:SF31. 1 hit.
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
PR00031. HTHREPRESSR.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and DNA-binding properties of the rat pancreatic beta-cell-specific factor Nkx6.1."
    Jorgensen M.C., Vestergard Petersen H., Ericson J., Madsen O.D., Serup P.
    FEBS Lett. 461:287-294(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Pancreatic islet.

Entry informationi

Entry nameiNKX61_RAT
AccessioniPrimary (citable) accession number: O35762
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.