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Protein

Isopentenyl-diphosphate Delta-isomerase 1

Gene

Idi1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP).

Catalytic activityi

Isopentenyl diphosphate = dimethylallyl diphosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: dimethylallyl diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes dimethylallyl diphosphate from isopentenyl diphosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Isopentenyl-diphosphate Delta-isomerase 1 (Idi1)
This subpathway is part of the pathway dimethylallyl diphosphate biosynthesis, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dimethylallyl diphosphate from isopentenyl diphosphate, the pathway dimethylallyl diphosphate biosynthesis and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Metal bindingi40MagnesiumBy similarity1
Metal bindingi51MagnesiumBy similarity1
Binding sitei70SubstrateBy similarity1
Binding sitei74SubstrateBy similarity1
Active sitei86By similarity1
Binding sitei87SubstrateBy similarity1
Metal bindingi146MagnesiumBy similarity1
Active sitei148By similarity1
Metal bindingi148MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processCholesterol biosynthesis, Cholesterol metabolism, Isoprene biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BRENDAi5.3.3.2. 5301.
UniPathwayiUPA00059; UER00104.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopentenyl-diphosphate Delta-isomerase 1 (EC:5.3.3.2)
Alternative name(s):
Isopentenyl pyrophosphate isomerase 1
Short name:
IPP isomerase 1
Short name:
IPPI1
Gene namesi
Name:Idi1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621835. Idi1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002052271 – 227Isopentenyl-diphosphate Delta-isomerase 1Add BLAST227

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei176N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO35760.
PRIDEiO35760.

PTM databases

iPTMnetiO35760.
PhosphoSitePlusiO35760.

Expressioni

Gene expression databases

GenevisibleiO35760. RN.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022389.

Structurei

3D structure databases

ProteinModelPortaliO35760.
SMRiO35760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 199Nudix hydrolasePROSITE-ProRule annotationAdd BLAST151

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi225 – 227Microbody targeting signal3

Sequence similaritiesi

Belongs to the IPP isomerase type 1 family.Curated

Phylogenomic databases

eggNOGiKOG0142. Eukaryota.
COG1443. LUCA.
HOGENOMiHOG000274106.
HOVERGENiHBG002995.
InParanoidiO35760.
KOiK01823.
OrthoDBiEOG091G0LWP.
PhylomeDBiO35760.
TreeFamiTF300129.

Family and domain databases

CDDicd02885. IPP_Isomerase. 1 hit.
InterProiView protein in InterPro
IPR011876. IsopentenylPP_isomerase_typ1.
IPR015797. NUDIX_hydrolase-like_dom_sf.
IPR000086. NUDIX_hydrolase_dom.
PANTHERiPTHR10885. PTHR10885. 1 hit.
PfamiView protein in Pfam
PF00293. NUDIX. 1 hit.
PIRSFiPIRSF018427. Isopntndiph_ism. 1 hit.
SUPFAMiSSF55811. SSF55811. 1 hit.
TIGRFAMsiTIGR02150. IPP_isom_1. 1 hit.
PROSITEiView protein in PROSITE
PS51462. NUDIX. 1 hit.

Sequencei

Sequence statusi: Complete.

O35760-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEINASNLD EKQVQLLAEM CILIDENDNK IGADTKKNCH LNENIDKGLI
60 70 80 90 100
HRAFSVFLFN TENKLLLQQR SDAKITFPGC FTNSCCSHPL NNPGELEEND
110 120 130 140 150
AMGVKRAAQR RLKAELGIPL EEVDLNEMNY LTRIYYKAQS DGIWGEHEID
160 170 180 190 200
YILFLRKNVT LNPDPNEIKS YCYVSKEELK EILKKEARGE IKLTPWFKII
210 220
ADAFLFKWWD NLNHLSPFVD HEKIHRM
Length:227
Mass (Da):26,396
Last modified:May 15, 2007 - v2
Checksum:i58943AED08D74DB0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110R → K in AAC53282 (PubMed:9228075).Curated1
Sequence conflicti193L → F in AAC53282 (PubMed:9228075).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF003835 mRNA. Translation: AAC53282.1.
BC089786 mRNA. Translation: AAH89786.2.
RefSeqiNP_445991.2. NM_053539.2.
UniGeneiRn.10780.

Genome annotation databases

GeneIDi89784.
KEGGirno:89784.
UCSCiRGD:621835. rat.

Similar proteinsi

Entry informationi

Entry nameiIDI1_RAT
AccessioniPrimary (citable) accession number: O35760
Secondary accession number(s): Q5FVT3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 15, 2007
Last modified: November 22, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families