O35744 (CH3L3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitinase-3-like protein 3

Alternative name(s):
ECF-L
Eosinophil chemotactic cytokine
Secreted protein Ym1

Gene names

Name:	Chi3l3
Synonyms:	Ym1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger. Binds chitin and heparin. Has no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy. Ref.1 Ref.2
Subcellular location	Secreted Ref.2.
Tissue specificity	Detected at low levels in bone marrow, spleen and thymus. Ref.1 Ref.2
Induction	Up-regulated in response to IL3 and IL4, during the inflammatory response and upon parasitic infection. Ref.2 Ref.4
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Biophysicochemical properties	pH dependence: Optimum pH is 4.5-5.0.

Ontologies

Keywords
Biological process	Inflammatory response
Cellular component	Secreted
Domain	Signal
Ligand	Chitin-binding Lectin
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	chitin catabolic process Inferred from electronic annotation. Source: InterPro inflammatory response Traceable author statement. Source: MGI
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from direct assay. Source: MGI extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from direct assay. Source: MGI cation binding Inferred from electronic annotation. Source: InterPro chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: InterPro sugar binding Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Ref.1 Ref.2

Chain

22 – 398

377

Chitinase-3-like protein 3

PRO_0000011970

Sites

Binding site

140

1

Saccharide Probable

Binding site

213

1

Saccharide Probable

Amino acid modifications

Disulfide bond

26 ↔ 51

Disulfide bond

49 ↔ 394

Disulfide bond

307 ↔ 372

Experimental info

Sequence conflict

105

1

P → S in BAA13458. Ref.1

Secondary structure

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398

Helix Strand Turn

Details...

Beta strand

23 – 29

7

Helix

30 – 34

5

Helix

37 – 39

3

Helix

43 – 45

3

Turn

48 – 50

3

Beta strand

52 – 62

11

Beta strand

65 – 67

3

Helix

73 – 82

10

Helix

83 – 85

3

Beta strand

91 – 97

7

Turn

99 – 101

3

Helix

104 – 110

7

Helix

113 – 129

17

Beta strand

134 – 138

5

Helix

150 – 173

24

Beta strand

179 – 184

6

Helix

188 – 194

7

Helix

197 – 203

7

Beta strand

205 – 209

5

Helix

217 – 219

3

Helix

236 – 240

5

Helix

243 – 252

10

Helix

257 – 259

3

Beta strand

260 – 274

15

Beta strand

284 – 288

5

Turn

293 – 295

3

Beta strand

300 – 302

3

Helix

303 – 311

9

Beta strand

315 – 319

5

Turn

320 – 323

4

Beta strand

324 – 329

6

Beta strand

332 – 335

4

Helix

339 – 351

13

Beta strand

356 – 360

5

Helix

362 – 364

3

Turn

370 – 372

3

Helix

378 – 386

9

Sequences

	Sequence		Length	Mass (Da)
	O35744 [UniParc]. Last modified March 1, 2001. Version 2. Checksum: C11187661B99D1D1	FASTA	398	44,458
	10 20 30 40 50 60 MAKLILVTGL AILLNVQLGS SYQLMCYYTS WAKDRPIEGS FKPGNIDPCL CTHLIYAFAG 70 80 90 100 110 120 MQNNEITYTH EQDLRDYEAL NGLKDKNTEL KTLLAIGGWK FGPAPFSAMV STPQNRQIFI 130 140 150 160 170 180 QSVIRFLRQY NFDGLNLDWQ YPGSRGSPPK DKHLFSVLVK EMRKAFEEES VEKDIPRLLL 190 200 210 220 230 240 TSTGAGIIDV IKSGYKIPEL SQSLDYIQVM TYDLHDPKDG YTGENSPLYK SPYDIGKSAD 250 260 270 280 290 300 LNVDSIISYW KDHGAASEKL IVGFPAYGHT FILSDPSKTG IGAPTISTGP PGKYTDESGL 310 320 330 340 350 360 LAYYEVCTFL NEGATEVWDA PQEVPYAYQG NEWVGYDNVR SFKLKAQWLK DNNLGGAVVW 370 380 390 PLDMDDFSGS FCHQRHFPLT STLKGDLNIH SASCKGPY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel eosinophil chemotactic cytokine (ECF-L) as a chitinase family protein." Owhashi M., Arita H., Hayai N. J. Biol. Chem. 275:1279-1286(2000) [PubMed: 10625674] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-39, FUNCTION, TISSUE SPECIFICITY. Tissue: Bone marrow.
[2]	"A macrophage protein, Ym1, transiently expressed during inflammation is a novel mammalian lectin." Chang N.-C.A., Hung S.-I., Hwa K.-Y., Kato I., Chen J.-E., Liu C.-H., Chang A.C. J. Biol. Chem. 276:17497-17506(2001) [PubMed: 11297523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-48; 109-134; 162-192 AND 210-225, FUNCTION, INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Macrophage.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart and Lung.
[4]	"TH2 cytokines and allergic challenge induce Ym1 expression in macrophages by a STAT6-dependent mechanism." Welch J.S., Escoubet-Lozach L., Sykes D.B., Liddiard K., Greaves D.R., Glass C.K. J. Biol. Chem. 277:42821-42829(2002) [PubMed: 12215441] [Abstract] Cited for: INDUCTION BY IL3 AND IL4.
[5]	"The crystal structure of a novel mammalian lectin, Ym1, suggests a saccharide binding site." Sun Y.-J., Chang N.-C.A., Hung S.-I., Chang A.C., Chou C.-C., Hsiao C.-D. J. Biol. Chem. 276:17507-17514(2001) [PubMed: 11278670] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D87757 mRNA. Translation: BAA13458.2.
M94584 mRNA. Translation: AAB62394.2.
BC061154 mRNA. Translation: AAH61154.1.

IPI

IPI00157508.

PIR

S27879.

RefSeq

NP_034022.2. NM_009892.2.

UniGene

Mm.387173.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E9L	X-ray	2.50	A	22-398	[»]
1VF8	X-ray	1.31	A	22-398	[»]

ProteinModelPortal

O35744.

SMR

O35744. Positions 22-394.

ModBase

Search...

Protein-protein interaction databases

STRING

O35744.

Protein family/group databases

CAZy

GH18. Glycoside Hydrolase Family 18.

Proteomic databases

PRIDE

O35744.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000063062; ENSMUSP00000053923; ENSMUSG00000040809.
ENSMUST00000168857; ENSMUSP00000130153; ENSMUSG00000040809.

GeneID

12655.

KEGG

mmu:12655.

Organism-specific databases

CTD

12655.

MGI

MGI:1330860. Chi3l3.

Phylogenomic databases

eggNOG

maNOG13249.

HOGENOM

HBG443716.

HOVERGEN

HBG011684.

InParanoid

O35744.

OMA

DGLWISY.

OrthoDB

EOG43JC6J.

PhylomeDB

O35744.

Gene expression databases

ArrayExpress

O35744.

Bgee

O35744.

CleanEx

MM_CHI3L3.

Genevestigator

O35744.

GermOnline

ENSMUSG00000040809. Mus musculus.

Family and domain databases

InterPro

IPR011583. Chitinase_II.
IPR001223. Glyco_hydro18cat.
IPR013781. Glyco_hydro_subgr_catalytic.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

Gene3D

G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits.

Pfam

PF00704. Glyco_hydro_18. 1 hit.
[Graphical view]

SMART

SM00636. Glyco_18. 1 hit.
[Graphical view]

SUPFAM

SSF51445. Glyco_hydro_cat. 1 hit.

ProtoNet

Search...

Other

NextBio

281876.

SOURCE

Search...

Entry information

Entry name

CH3L3_MOUSE

Accession

Primary (citable) accession number: O35744
Secondary accession number(s): P70201

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2001
Last modified:	September 21, 2011
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program