Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chitinase-like protein 3

Gene

Chil3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lectin that binds saccharides with a free amino group, such as glucosamine or galactosamine. Binding to oligomeric saccharides is much stronger than binding to mono- or disaccharides. Also binds chitin and heparin. Has weak hexosaminidase activity but no chitinase activity. Has chemotactic activity for T-lymphocytes, bone marrow cells and eosinophils. May play a role in inflammation and allergy.3 Publications

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Kineticsi

4-methylumbelliferone-N-acetylglucosamine (MU-(GlcNAc)1) is a GlcNAc2 analog.

  1. KM=120.8 µM for 4-methylumbelliferone-N-acetylglucosamine (at pH 4-4.5)1 Publication
  1. Vmax=0.023 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 4.5-5.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140ChitooligosaccharideCurated1
Binding sitei213ChitooligosaccharideCurated1

GO - Molecular functioni

  • beta-N-acetylhexosaminidase activity Source: MGI
  • carbohydrate binding Source: MGI
  • chitin binding Source: GO_Central

GO - Biological processi

  • chitin catabolic process Source: GO_Central
  • inflammatory response Source: MGI
  • polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Inflammatory response, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding, Lectin

Enzyme and pathway databases

ReactomeiR-MMU-71387. Metabolism of carbohydrates.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase-like protein 3 (EC:3.2.1.52)
Alternative name(s):
Beta-N-acetylhexosaminidase Ym1
Chitinase-3-like protein 3
ECF-L
Eosinophil chemotactic cytokine
Secreted protein Ym1
Gene namesi
Name:Chil3
Synonyms:Chi3l3, Ym1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1330860. Chil3.

Subcellular locationi

  • Secreted
  • Rough endoplasmic reticulum lumen
  • Nucleus envelope
  • Cytoplasm
  • Cytoplasmic granule

  • Note: Predominantly localizes to the lumen of rough endoplasmic reticulum (rER) and nuclear envelope in alveolar macrophages. Localizes to the dilated lumen of rER in immature neutrophils in spleen and in cytoplasmic granules in peritoneal neutrophils. Detected in needle-shaped crystals present in the cytoplasm of bone marrow macrophages.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: MGI
  • extracellular region Source: GO_Central
  • nuclear envelope Source: UniProtKB-SubCell
  • rough endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1795141.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 212 PublicationsAdd BLAST21
ChainiPRO_000001197022 – 398Chitinase-like protein 3Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 51
Disulfide bondi49 ↔ 394
Disulfide bondi307 ↔ 372

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO35744.
PeptideAtlasiO35744.
PRIDEiO35744.

Expressioni

Tissue specificityi

Expressed in peritoneal cavity macrophages and in peritoneal and bone marrow-derived neutrophils. Abundantly expressed in bone marrow, with moderate levels detected in gastric antrum, spleen and in alveolar macrophages in lung. Not detected in brain, heart, liver, kidney, stomach, intestine, skeletal muscle, ovary, testis, thymus and lymph nodes (at protein level). Detected at low levels in bone marrow, spleen, thymus and lung. Barely detectable in intestine, kidney and coecum.6 Publications

Developmental stagei

In yolk sac, first detected at low levels at E8.5, with significant expression detected at E10.5 in myeloid precursor cells. In liver, expressed from E16.5 to P7.5 with highest levels detected from E18.5 to P0.5. In spleen, first detected at E16.5, with peak levels detected at E18.5 and P0.5 and expression persisting through the spleen maturation to the adult stage. In bone marrow, high expression levels detected from E16.5 until adulthood. In lung, first detected around the time of birth, with levels increasing significantly from P14.5 towards adulthood.1 Publication

Inductioni

Up-regulated in response to IL3 and IL4, during the inflammatory response and upon parasitic infection.4 Publications

Gene expression databases

BgeeiENSMUSG00000040809.
CleanExiMM_CHI3L3.
ExpressionAtlasiO35744. baseline and differential.
GenevisibleiO35744. MM.

Interactioni

Protein-protein interaction databases

IntActiO35744. 1 interactor.
MINTiMINT-4090737.
STRINGi10090.ENSMUSP00000053923.

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 29Combined sources7
Helixi30 – 34Combined sources5
Helixi37 – 39Combined sources3
Helixi43 – 45Combined sources3
Turni48 – 50Combined sources3
Beta strandi52 – 62Combined sources11
Beta strandi65 – 67Combined sources3
Helixi73 – 82Combined sources10
Helixi83 – 85Combined sources3
Beta strandi91 – 97Combined sources7
Turni99 – 101Combined sources3
Helixi104 – 110Combined sources7
Helixi113 – 129Combined sources17
Beta strandi134 – 138Combined sources5
Beta strandi142 – 144Combined sources3
Helixi150 – 173Combined sources24
Beta strandi179 – 184Combined sources6
Helixi188 – 194Combined sources7
Helixi197 – 203Combined sources7
Beta strandi205 – 209Combined sources5
Helixi217 – 219Combined sources3
Helixi236 – 240Combined sources5
Helixi243 – 252Combined sources10
Helixi257 – 259Combined sources3
Beta strandi260 – 274Combined sources15
Beta strandi284 – 288Combined sources5
Turni293 – 295Combined sources3
Beta strandi300 – 302Combined sources3
Helixi303 – 311Combined sources9
Beta strandi315 – 319Combined sources5
Turni320 – 323Combined sources4
Beta strandi324 – 329Combined sources6
Beta strandi332 – 335Combined sources4
Helixi339 – 351Combined sources13
Beta strandi356 – 360Combined sources5
Helixi362 – 364Combined sources3
Turni370 – 372Combined sources3
Helixi378 – 386Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E9LX-ray2.50A22-398[»]
1VF8X-ray1.31A22-398[»]
ProteinModelPortaliO35744.
SMRiO35744.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO35744.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
GeneTreeiENSGT00550000074323.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiO35744.
KOiK17524.
OMAiIISYWKD.
OrthoDBiEOG091G014W.
PhylomeDBiO35744.
TreeFamiTF315610.

Family and domain databases

Gene3Di3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLILVTGL AILLNVQLGS SYQLMCYYTS WAKDRPIEGS FKPGNIDPCL
60 70 80 90 100
CTHLIYAFAG MQNNEITYTH EQDLRDYEAL NGLKDKNTEL KTLLAIGGWK
110 120 130 140 150
FGPAPFSAMV STPQNRQIFI QSVIRFLRQY NFDGLNLDWQ YPGSRGSPPK
160 170 180 190 200
DKHLFSVLVK EMRKAFEEES VEKDIPRLLL TSTGAGIIDV IKSGYKIPEL
210 220 230 240 250
SQSLDYIQVM TYDLHDPKDG YTGENSPLYK SPYDIGKSAD LNVDSIISYW
260 270 280 290 300
KDHGAASEKL IVGFPAYGHT FILSDPSKTG IGAPTISTGP PGKYTDESGL
310 320 330 340 350
LAYYEVCTFL NEGATEVWDA PQEVPYAYQG NEWVGYDNVR SFKLKAQWLK
360 370 380 390
DNNLGGAVVW PLDMDDFSGS FCHQRHFPLT STLKGDLNIH SASCKGPY
Length:398
Mass (Da):44,458
Last modified:March 1, 2001 - v2
Checksum:iC11187661B99D1D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105P → S no nucleotide entry (PubMed:9828134).Curated1
Sequence conflicti105P → S in BAA13458 (PubMed:10625674).Curated1
Sequence conflicti257S → P in BAE23385 (PubMed:11297523).Curated1
Sequence conflicti361P → R no nucleotide entry (PubMed:9828134).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87757 mRNA. Translation: BAA13458.2.
M94584 mRNA. Translation: AAB62394.2.
AK137503 mRNA. Translation: BAE23385.1.
AK154420 mRNA. Translation: BAE32572.1.
BC061154 mRNA. Translation: AAH61154.1.
U56900 mRNA. Translation: AAB01230.1.
CCDSiCCDS17718.1.
PIRiS27879.
RefSeqiNP_034022.2. NM_009892.3.
UniGeneiMm.387173.

Genome annotation databases

EnsembliENSMUST00000063062; ENSMUSP00000053923; ENSMUSG00000040809.
GeneIDi12655.
KEGGimmu:12655.
UCSCiuc008qvw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87757 mRNA. Translation: BAA13458.2.
M94584 mRNA. Translation: AAB62394.2.
AK137503 mRNA. Translation: BAE23385.1.
AK154420 mRNA. Translation: BAE32572.1.
BC061154 mRNA. Translation: AAH61154.1.
U56900 mRNA. Translation: AAB01230.1.
CCDSiCCDS17718.1.
PIRiS27879.
RefSeqiNP_034022.2. NM_009892.3.
UniGeneiMm.387173.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E9LX-ray2.50A22-398[»]
1VF8X-ray1.31A22-398[»]
ProteinModelPortaliO35744.
SMRiO35744.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35744. 1 interactor.
MINTiMINT-4090737.
STRINGi10090.ENSMUSP00000053923.

Chemistry databases

ChEMBLiCHEMBL1795141.

Protein family/group databases

CAZyiGH18. Glycoside Hydrolase Family 18.

Proteomic databases

PaxDbiO35744.
PeptideAtlasiO35744.
PRIDEiO35744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063062; ENSMUSP00000053923; ENSMUSG00000040809.
GeneIDi12655.
KEGGimmu:12655.
UCSCiuc008qvw.2. mouse.

Organism-specific databases

CTDi12655.
MGIiMGI:1330860. Chil3.

Phylogenomic databases

eggNOGiKOG2806. Eukaryota.
COG3325. LUCA.
GeneTreeiENSGT00550000074323.
HOGENOMiHOG000111109.
HOVERGENiHBG011684.
InParanoidiO35744.
KOiK17524.
OMAiIISYWKD.
OrthoDBiEOG091G014W.
PhylomeDBiO35744.
TreeFamiTF315610.

Enzyme and pathway databases

ReactomeiR-MMU-71387. Metabolism of carbohydrates.

Miscellaneous databases

EvolutionaryTraceiO35744.
PROiO35744.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040809.
CleanExiMM_CHI3L3.
ExpressionAtlasiO35744. baseline and differential.
GenevisibleiO35744. MM.

Family and domain databases

Gene3Di3.10.50.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR011583. Chitinase_II.
IPR029070. Chitinase_insertion.
IPR001223. Glyco_hydro18_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00704. Glyco_hydro_18. 1 hit.
[Graphical view]
SMARTiSM00636. Glyco_18. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF54556. SSF54556. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCHIL3_MOUSE
AccessioniPrimary (citable) accession number: O35744
Secondary accession number(s): P70201
, Q3U462, Q3UV87, Q61201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.