Reviewed, UniProtKB/Swiss-Prot O35728 (CP4AE_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 4A14
Alternative name(s):
    CYPIVA14
    Lauric acid omega-hydroxylase 3
    EC=1.14.15.3
    P450-LA-omega 3

Gene names

Name:

Cyp4a14

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	Octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O. UniProtKB P20817
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Very low level in liver, kidney and spleen. Ref.1
Induction	By peroxisome proliferator methylclofenapate; 1000-fold in liver, 10-fold in kidney. Ref.1
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence BC089609 differs from that shown. Reason: Erroneous termination at position 365. Translated as Gln.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkane 1-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 4	4	Removed in mature form By similarity UniProtKB P20817		PRO_0000046058
	Chain	5 – 507	503	Cytochrome P450 4A14 UniProtKB P20817		PRO_0000046059
Sites
	Metal binding	454	1	Iron (heme axial ligand) By similarity UniProtKB P20817
	Binding site	318	1	Heme (covalent; via 1 link) By similarity UniProtKB P20817
Amino acid modifications
	Modified residue	437	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	231	1	N → D in BAE28849. Ref.2
	Sequence conflict	278	1	Q → R in BC089609. Ref.4
	Sequence conflict	401	1	S → F in BC089609. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	O35728-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: EB46205838D4549C	FASTA	507	58,720
	10 20 30 40 50 60 MGFFLFSPTR YLDGISGFFQ WAFLLSLFLV LFKAVQFYLR RQWLLKTLQH FPCMPSHWLW 70 80 90 100 110 120 GHHLKDKELQ QILIWVEKFP SACLQCLSGS NIRVLLYDPD YVKVVLGRSD PKASGIYQFF 130 140 150 160 170 180 APWIGYGLLL LNGKKWFQHR RMLTPAFHYD ILKPYVKIMA DSVNIMLDKW EKLDGQDHPL 190 200 210 220 230 240 EIFHCVSLMT LDTVMKCAFS YQGSVQLDEN SKLYTKAVED LNNLTFFRLR NAFYKYNIIY 250 260 270 280 290 300 NMSSDGRLSH HACQIAHEHT DGVIKMRKSQ LQNEEELQKA RKKRHLDFLD ILLFARMEDR 310 320 330 340 350 360 NSLSDEDLRA EVDTFMFEGH DTTASGISWI FYALATHPEH QQRCREEVQS ILGDGTSVTW 370 380 390 400 410 420 DHLGQMPYTT MCIKEALRLY PPVISVSREL SSPVTFPDGR SIPKGITATI SIYGLHHNPR 430 440 450 460 470 480 FWPNPKVFDP SRFAPDSSHH SHAYLPFSGG SRNCIGKQFA MNELKVAVAL TLLRFELLPD 490 500 PTRIPVPIAR LVLKSKNGIH LCLKKLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel murine P-450 gene, Cyp4a14, is part of a cluster of Cyp4a and Cyp4b, but not of CYP4F, genes in mouse and humans." Heng Y.M., Kuo C.-W.S., Jones P.S., Savory R., Shultz R.M., Tomlinson S.R., Gray T.J.B., Bell D.R. Biochem. J. 325:741-749(1997) [PubMed: 9271096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION. Strain: C57BL/6 X CBA.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney and Liver.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y11638 , Y11639, Y11640, Y11641, Y11642 Genomic DNA. Translation: CAA72345.1. AK149392 mRNA. Translation: BAE28849.1. AK165452 mRNA. Translation: BAE38193.1. AL627182 Genomic DNA. Translation: CAM21089.1. BC089609 mRNA. No translation available.
IPI	IPI00133877.
RefSeq	NP_031848.1.
UniGene	Mm.250901
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O35728.
PTM databases
PhosphoSite	O35728.
Proteomic databases
PRIDE	O35728.
Genome annotation databases
Ensembl	ENSMUST00000030487; ENSMUSP00000030487; ENSMUSG00000028715; Mus musculus. [Genome view]
GeneID	13119.
KEGG	mmu:13119.
UCSC	uc008uev.1. mouse.
Organism-specific databases
CTD	13119.
MGI	MGI:1096550. Cyp4a14.
Phylogenomic databases
HOGENOM	O35728.
HOVERGEN	O35728.
OMA	RSIPKGI.
Enzyme and pathway databases
BRENDA	1.14.15.3. 244.
Gene expression databases
ArrayExpress	O35728.
Bgee	O35728.
Genevestigator	O35728.
GermOnline	ENSMUSG00000028715. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	283150.
SOURCE	Search...

Entry information

Entry name

CP4AE_MOUSE

Accession

Primary (citable) accession number: O35728
Secondary accession number(s): A2A975, Q3UER2, Q5EBH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)