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Protein

Cytochrome P450 4A14

Gene

Cyp4a14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.Curated

Catalytic activityi

A long-chain fatty acid + NADPH + O2 = an omega-hydroxy-long-chain fatty acid + NADP+ + H2O.By similarity
Octane + 2 reduced rubredoxin + O2 + 2 H+ = 1-octanol + 2 oxidized rubredoxin + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei318 – 3181Heme (covalent; via 1 link)By similarity
Metal bindingi454 – 4541Iron (heme axial ligand)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-MMU-211935. Fatty acids.
R-MMU-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Chemistry

SwissLipidsiSLP:000000483.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 4A14
Alternative name(s):
CYPIVA14
Cytochrome P450-LA-omega 3
Lauric acid omega-hydroxylase 3
Long-chain fatty acid omega-monooxygenase (EC:1.14.13.205)
Gene namesi
Name:Cyp4a14Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1096550. Cyp4a14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 44Removed in mature formBy similarityPRO_0000046058
Chaini5 – 507503Cytochrome P450 4A14By similarityPRO_0000046059Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei437 – 4371PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35728.
PaxDbiO35728.
PRIDEiO35728.

PTM databases

iPTMnetiO35728.
PhosphoSiteiO35728.

Expressioni

Tissue specificityi

Very low level in liver, kidney and spleen.1 Publication

Inductioni

By peroxisome proliferator methylclofenapate; 1000-fold in liver, 10-fold in kidney.1 Publication

Gene expression databases

BgeeiO35728.
GenevisibleiO35728. MM.

Interactioni

Protein-protein interaction databases

IntActiO35728. 1 interaction.
MINTiMINT-4104845.
STRINGi10090.ENSMUSP00000030487.

Structurei

3D structure databases

ProteinModelPortaliO35728.
SMRiO35728. Positions 98-507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Sequence analysis

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiO35728.
KOiK07425.
OrthoDBiEOG7CNZFK.
PhylomeDBiO35728.
TreeFamiTF105088.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFFLFSPTR YLDGISGFFQ WAFLLSLFLV LFKAVQFYLR RQWLLKTLQH
60 70 80 90 100
FPCMPSHWLW GHHLKDKELQ QILIWVEKFP SACLQCLSGS NIRVLLYDPD
110 120 130 140 150
YVKVVLGRSD PKASGIYQFF APWIGYGLLL LNGKKWFQHR RMLTPAFHYD
160 170 180 190 200
ILKPYVKIMA DSVNIMLDKW EKLDGQDHPL EIFHCVSLMT LDTVMKCAFS
210 220 230 240 250
YQGSVQLDEN SKLYTKAVED LNNLTFFRLR NAFYKYNIIY NMSSDGRLSH
260 270 280 290 300
HACQIAHEHT DGVIKMRKSQ LQNEEELQKA RKKRHLDFLD ILLFARMEDR
310 320 330 340 350
NSLSDEDLRA EVDTFMFEGH DTTASGISWI FYALATHPEH QQRCREEVQS
360 370 380 390 400
ILGDGTSVTW DHLGQMPYTT MCIKEALRLY PPVISVSREL SSPVTFPDGR
410 420 430 440 450
SIPKGITATI SIYGLHHNPR FWPNPKVFDP SRFAPDSSHH SHAYLPFSGG
460 470 480 490 500
SRNCIGKQFA MNELKVAVAL TLLRFELLPD PTRIPVPIAR LVLKSKNGIH

LCLKKLR
Length:507
Mass (Da):58,720
Last modified:January 1, 1998 - v1
Checksum:iEB46205838D4549C
GO

Sequence cautioni

The sequence BC089609 differs from that shown. Reason: Erroneous termination at position 365. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti231 – 2311N → D in BAE28849 (PubMed:16141072).Curated
Sequence conflicti278 – 2781Q → R in BC089609 (PubMed:15489334).Curated
Sequence conflicti401 – 4011S → F in BC089609 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11638
, Y11639, Y11640, Y11641, Y11642 Genomic DNA. Translation: CAA72345.1.
AK149392 mRNA. Translation: BAE28849.1.
AK165452 mRNA. Translation: BAE38193.1.
AL627182 Genomic DNA. Translation: CAM21089.1.
BC089609 mRNA. No translation available.
CCDSiCCDS18491.1.
RefSeqiNP_031848.1. NM_007822.2.
UniGeneiMm.250901.

Genome annotation databases

EnsembliENSMUST00000030487; ENSMUSP00000030487; ENSMUSG00000028715.
GeneIDi13119.
KEGGimmu:13119.
UCSCiuc008uev.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11638
, Y11639, Y11640, Y11641, Y11642 Genomic DNA. Translation: CAA72345.1.
AK149392 mRNA. Translation: BAE28849.1.
AK165452 mRNA. Translation: BAE38193.1.
AL627182 Genomic DNA. Translation: CAM21089.1.
BC089609 mRNA. No translation available.
CCDSiCCDS18491.1.
RefSeqiNP_031848.1. NM_007822.2.
UniGeneiMm.250901.

3D structure databases

ProteinModelPortaliO35728.
SMRiO35728. Positions 98-507.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35728. 1 interaction.
MINTiMINT-4104845.
STRINGi10090.ENSMUSP00000030487.

Chemistry

SwissLipidsiSLP:000000483.

PTM databases

iPTMnetiO35728.
PhosphoSiteiO35728.

Proteomic databases

EPDiO35728.
PaxDbiO35728.
PRIDEiO35728.

Protocols and materials databases

DNASUi13119.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030487; ENSMUSP00000030487; ENSMUSG00000028715.
GeneIDi13119.
KEGGimmu:13119.
UCSCiuc008uev.2. mouse.

Organism-specific databases

CTDi13119.
MGIiMGI:1096550. Cyp4a14.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00760000118816.
HOGENOMiHOG000233833.
HOVERGENiHBG000182.
InParanoidiO35728.
KOiK07425.
OrthoDBiEOG7CNZFK.
PhylomeDBiO35728.
TreeFamiTF105088.

Enzyme and pathway databases

ReactomeiR-MMU-211935. Fatty acids.
R-MMU-2142816. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).

Miscellaneous databases

NextBioi283150.
PROiO35728.
SOURCEiSearch...

Gene expression databases

BgeeiO35728.
GenevisibleiO35728. MM.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel murine P-450 gene, Cyp4a14, is part of a cluster of Cyp4a and Cyp4b, but not of CYP4F, genes in mouse and humans."
    Heng Y.M., Kuo C.-W.S., Jones P.S., Savory R., Shultz R.M., Tomlinson S.R., Gray T.J.B., Bell D.R.
    Biochem. J. 325:741-749(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: C57BL/6 X CBAImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6JImported.
    Tissue: KidneyImported and LiverImported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Liver.

Entry informationi

Entry nameiCP4AE_MOUSE
AccessioniPrimary (citable) accession number: O35728
Secondary accession number(s): A2A975, Q3UER2, Q5EBH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: January 1, 1998
Last modified: March 16, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.