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Protein

DNA repair protein RAD51 homolog 2

Gene

Rad51b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158ATPSequence Analysis

GO - Molecular functioni

GO - Biological processi

  • blastocyst growth Source: MGI
  • double-strand break repair via homologous recombination Source: UniProtKB
  • in utero embryonic development Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
  • somite development Source: MGI
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 2
Short name:
R51H2
Alternative name(s):
RAD51 homolog B
RAD51-like protein 1
Gene namesi
Name:Rad51b
Synonyms:Rad51l1, Rec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1099436. Rad51b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350DNA repair protein RAD51 homolog 2PRO_0000122940Add
BLAST

Proteomic databases

MaxQBiO35719.
PRIDEiO35719.

PTM databases

PhosphoSiteiO35719.

Expressioni

Tissue specificityi

Expressed in a wide range of tissues.

Gene expression databases

BgeeiO35719.
CleanExiMM_RAD51L1.
ExpressionAtlasiO35719. baseline and differential.
GenevisibleiO35719. MM.

Interactioni

Subunit structurei

Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. The BCDX2 subcomplex RAD51B:RAD51C interacts with RAD51. Interacts with SWSAP1; involved in homologous recombination repair (By similarity).By similarity

Protein-protein interaction databases

BioGridi202566. 1 interaction.
STRINGi10090.ENSMUSP00000078490.

Structurei

3D structure databases

ProteinModelPortaliO35719.
SMRiO35719. Positions 10-291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7575Interaction with RAD51CBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0468.
GeneTreeiENSGT00770000120539.
HOGENOMiHOG000154062.
HOVERGENiHBG061476.
InParanoidiO35719.
KOiK10869.
OMAiNRYQIVT.
OrthoDBiEOG70PBZC.
PhylomeDBiO35719.
TreeFamiTF101219.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR027417. P-loop_NTPase.
IPR030548. RAD51B.
IPR020588. RecA_ATP-bd.
[Graphical view]
PANTHERiPTHR22942:SF15. PTHR22942:SF15. 1 hit.
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35719-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSKKLRRVG LSPELCDRLS RYQIVNCQHF LSLSPLELMK VTGLSYRGVH
60 70 80 90 100
ELLHTVSKAC APQMQTAYEL KTRRSAHLSP AFLSTTLCAL DEALHGGVPC
110 120 130 140 150
GSLTEITGPP GCGKTQFCIM MSVLATLPTS LGGLEGAVVY IDTESAFTAE
160 170 180 190 200
RLVEIAESRF PQYFNTEEKL LLTSSRVHLC RELTCEGLLQ RLESLEEEII
210 220 230 240 250
SKGVKLVIVD SIASVVRKEF DPKLQGNIKE RNKFLGKGAS LLKYLAGEFS
260 270 280 290 300
IPVILTNQIT THLSGALPSQ ADLVSPADDL SLSEGTSGSS CLVAALGNTW
310 320 330 340 350
GHCVNTRLIL QYLDSERRQI LIAKSPLAAF TSFVYTIKGE GLVLQGHERP
Length:350
Mass (Da):38,152
Last modified:November 7, 2003 - v2
Checksum:i2DB79F8D60C0529D
GO
Isoform 2 (identifier: O35719-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-258: ILTNQ → TFQPI
     259-350: Missing.

Note: No experimental confirmation available.
Show »
Length:258
Mass (Da):28,421
Checksum:i94C2067795B15B37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231Q → L in AAB63359 (PubMed:9207106).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei254 – 2585ILTNQ → TFQPI in isoform 2. 1 PublicationVSP_008869
Alternative sequencei259 – 35092Missing in isoform 2. 1 PublicationVSP_008870Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92068 mRNA. Translation: AAB63359.1.
AK015771 mRNA. Translation: BAB29970.1.
AK032913 mRNA. Translation: BAC28084.1.
CCDSiCCDS56845.1. [O35719-2]
RefSeqiNP_001239491.1. NM_001252562.1. [O35719-2]
NP_033040.2. NM_009014.3. [O35719-1]
XP_011242347.1. XM_011244045.1. [O35719-2]
UniGeneiMm.341756.

Genome annotation databases

EnsembliENSMUST00000079533; ENSMUSP00000078490; ENSMUSG00000059060. [O35719-2]
GeneIDi19363.
KEGGimmu:19363.
UCSCiuc007oai.1. mouse. [O35719-1]
uc007oaj.1. mouse. [O35719-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92068 mRNA. Translation: AAB63359.1.
AK015771 mRNA. Translation: BAB29970.1.
AK032913 mRNA. Translation: BAC28084.1.
CCDSiCCDS56845.1. [O35719-2]
RefSeqiNP_001239491.1. NM_001252562.1. [O35719-2]
NP_033040.2. NM_009014.3. [O35719-1]
XP_011242347.1. XM_011244045.1. [O35719-2]
UniGeneiMm.341756.

3D structure databases

ProteinModelPortaliO35719.
SMRiO35719. Positions 10-291.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202566. 1 interaction.
STRINGi10090.ENSMUSP00000078490.

PTM databases

PhosphoSiteiO35719.

Proteomic databases

MaxQBiO35719.
PRIDEiO35719.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079533; ENSMUSP00000078490; ENSMUSG00000059060. [O35719-2]
GeneIDi19363.
KEGGimmu:19363.
UCSCiuc007oai.1. mouse. [O35719-1]
uc007oaj.1. mouse. [O35719-2]

Organism-specific databases

CTDi5890.
MGIiMGI:1099436. Rad51b.

Phylogenomic databases

eggNOGiCOG0468.
GeneTreeiENSGT00770000120539.
HOGENOMiHOG000154062.
HOVERGENiHBG061476.
InParanoidiO35719.
KOiK10869.
OMAiNRYQIVT.
OrthoDBiEOG70PBZC.
PhylomeDBiO35719.
TreeFamiTF101219.

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSiRad51b. mouse.
NextBioi296429.
PROiO35719.
SOURCEiSearch...

Gene expression databases

BgeeiO35719.
CleanExiMM_RAD51L1.
ExpressionAtlasiO35719. baseline and differential.
GenevisibleiO35719. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR027417. P-loop_NTPase.
IPR030548. RAD51B.
IPR020588. RecA_ATP-bd.
[Graphical view]
PANTHERiPTHR22942:SF15. PTHR22942:SF15. 1 hit.
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis."
    Rice M.C., Smith S.T., Bullrich F., Havre P., Kmiec E.B.
    Proc. Natl. Acad. Sci. U.S.A. 94:7417-7422(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Mesonephros.

Entry informationi

Entry nameiRA51B_MOUSE
AccessioniPrimary (citable) accession number: O35719
Secondary accession number(s): Q8BSH6, Q9D555
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 7, 2003
Last modified: July 22, 2015
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.