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O35719 (RA51B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair protein RAD51 homolog 2

Short name=R51H2
Alternative name(s):
RAD51 homolog B
RAD51-like protein 1
Gene names
Name:Rad51b
Synonyms:Rad51l1, Rec2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway By similarity.

Subunit structure

Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. The BCDX2 subcomplex RAD51B:RAD51C interacts with RAD51. Interacts with SWSAP1; involved in homologous recombination repair By similarity.

Subcellular location

Nucleus By similarity.

Tissue specificity

Expressed in a wide range of tissues.

Sequence similarities

Belongs to the RecA family. RAD51 subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35719-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35719-2)

The sequence of this isoform differs from the canonical sequence as follows:
     254-258: ILTNQ → TFQPI
     259-350: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350DNA repair protein RAD51 homolog 2
PRO_0000122940

Regions

Nucleotide binding108 – 1158ATP Potential
Region1 – 7575Interaction with RAD51C By similarity

Natural variations

Alternative sequence254 – 2585ILTNQ → TFQPI in isoform 2.
VSP_008869
Alternative sequence259 – 35092Missing in isoform 2.
VSP_008870

Experimental info

Sequence conflict231Q → L in AAB63359. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 7, 2003. Version 2.
Checksum: 2DB79F8D60C0529D

FASTA35038,152
        10         20         30         40         50         60 
MSSKKLRRVG LSPELCDRLS RYQIVNCQHF LSLSPLELMK VTGLSYRGVH ELLHTVSKAC 

        70         80         90        100        110        120 
APQMQTAYEL KTRRSAHLSP AFLSTTLCAL DEALHGGVPC GSLTEITGPP GCGKTQFCIM 

       130        140        150        160        170        180 
MSVLATLPTS LGGLEGAVVY IDTESAFTAE RLVEIAESRF PQYFNTEEKL LLTSSRVHLC 

       190        200        210        220        230        240 
RELTCEGLLQ RLESLEEEII SKGVKLVIVD SIASVVRKEF DPKLQGNIKE RNKFLGKGAS 

       250        260        270        280        290        300 
LLKYLAGEFS IPVILTNQIT THLSGALPSQ ADLVSPADDL SLSEGTSGSS CLVAALGNTW 

       310        320        330        340        350 
GHCVNTRLIL QYLDSERRQI LIAKSPLAAF TSFVYTIKGE GLVLQGHERP 

« Hide

Isoform 2 [UniParc].

Checksum: 94C2067795B15B37
Show »

FASTA25828,421

References

« Hide 'large scale' references
[1]"Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis."
Rice M.C., Smith S.T., Bullrich F., Havre P., Kmiec E.B.
Proc. Natl. Acad. Sci. U.S.A. 94:7417-7422(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Mesonephros.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U92068 mRNA. Translation: AAB63359.1.
AK015771 mRNA. Translation: BAB29970.1.
AK032913 mRNA. Translation: BAC28084.1.
CCDSCCDS56845.1. [O35719-2]
RefSeqNP_001239491.1. NM_001252562.1. [O35719-2]
NP_033040.2. NM_009014.3. [O35719-1]
UniGeneMm.341756.

3D structure databases

ProteinModelPortalO35719.
SMRO35719. Positions 10-291.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202566. 1 interaction.

PTM databases

PhosphoSiteO35719.

Proteomic databases

PRIDEO35719.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000079533; ENSMUSP00000078490; ENSMUSG00000059060. [O35719-2]
GeneID19363.
KEGGmmu:19363.
UCSCuc007oai.1. mouse. [O35719-1]
uc007oaj.1. mouse. [O35719-2]

Organism-specific databases

CTD5890.
MGIMGI:1099436. Rad51b.

Phylogenomic databases

eggNOGCOG0468.
GeneTreeENSGT00750000117586.
HOGENOMHOG000154062.
HOVERGENHBG061476.
InParanoidO35719.
KOK10869.
OMACIMMSVL.
OrthoDBEOG70PBZC.
PhylomeDBO35719.
TreeFamTF101219.

Gene expression databases

ArrayExpressO35719.
BgeeO35719.
CleanExMM_RAD51L1.
GenevestigatorO35719.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view]
PfamPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFPIRSF005856. Rad51. 1 hit.
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS50162. RECA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296429.
PROO35719.
SOURCESearch...

Entry information

Entry nameRA51B_MOUSE
AccessionPrimary (citable) accession number: O35719
Secondary accession number(s): Q8BSH6, Q9D555
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 7, 2003
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot