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O35719

- RA51B_MOUSE

UniProt

O35719 - RA51B_MOUSE

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Protein
DNA repair protein RAD51 homolog 2
Gene
Rad51b, Rad51l1, Rec2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA-dependent ATPase activity Source: InterPro
  3. double-stranded DNA binding Source: UniProtKB
  4. single-stranded DNA binding Source: UniProtKB

GO - Biological processi

  1. double-strand break repair via homologous recombination Source: UniProtKB
  2. positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD51 homolog 2
Short name:
R51H2
Alternative name(s):
RAD51 homolog B
RAD51-like protein 1
Gene namesi
Name:Rad51b
Synonyms:Rad51l1, Rec2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1099436. Rad51b.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. Rad51B-Rad51C-Rad51D-XRCC2 complex Source: UniProtKB
  2. replication fork Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 350350DNA repair protein RAD51 homolog 2
PRO_0000122940Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO35719.

PTM databases

PhosphoSiteiO35719.

Expressioni

Tissue specificityi

Expressed in a wide range of tissues.

Gene expression databases

ArrayExpressiO35719.
BgeeiO35719.
CleanExiMM_RAD51L1.
GenevestigatoriO35719.

Interactioni

Subunit structurei

Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. The BCDX2 subcomplex RAD51B:RAD51C interacts with RAD51. Interacts with SWSAP1; involved in homologous recombination repair By similarity.

Protein-protein interaction databases

BioGridi202566. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO35719.
SMRiO35719. Positions 10-291.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7575Interaction with RAD51C By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the RecA family. RAD51 subfamily.

Phylogenomic databases

eggNOGiCOG0468.
GeneTreeiENSGT00750000117586.
HOGENOMiHOG000154062.
HOVERGENiHBG061476.
InParanoidiO35719.
KOiK10869.
OMAiCIMMSVL.
OrthoDBiEOG70PBZC.
PhylomeDBiO35719.
TreeFamiTF101219.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view]
PfamiPF08423. Rad51. 1 hit.
[Graphical view]
PIRSFiPIRSF005856. Rad51. 1 hit.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50162. RECA_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O35719-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSSKKLRRVG LSPELCDRLS RYQIVNCQHF LSLSPLELMK VTGLSYRGVH    50
ELLHTVSKAC APQMQTAYEL KTRRSAHLSP AFLSTTLCAL DEALHGGVPC 100
GSLTEITGPP GCGKTQFCIM MSVLATLPTS LGGLEGAVVY IDTESAFTAE 150
RLVEIAESRF PQYFNTEEKL LLTSSRVHLC RELTCEGLLQ RLESLEEEII 200
SKGVKLVIVD SIASVVRKEF DPKLQGNIKE RNKFLGKGAS LLKYLAGEFS 250
IPVILTNQIT THLSGALPSQ ADLVSPADDL SLSEGTSGSS CLVAALGNTW 300
GHCVNTRLIL QYLDSERRQI LIAKSPLAAF TSFVYTIKGE GLVLQGHERP 350
Length:350
Mass (Da):38,152
Last modified:November 7, 2003 - v2
Checksum:i2DB79F8D60C0529D
GO
Isoform 2 (identifier: O35719-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     254-258: ILTNQ → TFQPI
     259-350: Missing.

Note: No experimental confirmation available.

Show »
Length:258
Mass (Da):28,421
Checksum:i94C2067795B15B37
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei254 – 2585ILTNQ → TFQPI in isoform 2.
VSP_008869
Alternative sequencei259 – 35092Missing in isoform 2.
VSP_008870Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231Q → L in AAB63359. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92068 mRNA. Translation: AAB63359.1.
AK015771 mRNA. Translation: BAB29970.1.
AK032913 mRNA. Translation: BAC28084.1.
CCDSiCCDS56845.1. [O35719-2]
RefSeqiNP_001239491.1. NM_001252562.1. [O35719-2]
NP_033040.2. NM_009014.3. [O35719-1]
UniGeneiMm.341756.

Genome annotation databases

EnsembliENSMUST00000079533; ENSMUSP00000078490; ENSMUSG00000059060. [O35719-2]
GeneIDi19363.
KEGGimmu:19363.
UCSCiuc007oai.1. mouse. [O35719-1]
uc007oaj.1. mouse. [O35719-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U92068 mRNA. Translation: AAB63359.1 .
AK015771 mRNA. Translation: BAB29970.1 .
AK032913 mRNA. Translation: BAC28084.1 .
CCDSi CCDS56845.1. [O35719-2 ]
RefSeqi NP_001239491.1. NM_001252562.1. [O35719-2 ]
NP_033040.2. NM_009014.3. [O35719-1 ]
UniGenei Mm.341756.

3D structure databases

ProteinModelPortali O35719.
SMRi O35719. Positions 10-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202566. 1 interaction.

PTM databases

PhosphoSitei O35719.

Proteomic databases

PRIDEi O35719.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000079533 ; ENSMUSP00000078490 ; ENSMUSG00000059060 . [O35719-2 ]
GeneIDi 19363.
KEGGi mmu:19363.
UCSCi uc007oai.1. mouse. [O35719-1 ]
uc007oaj.1. mouse. [O35719-2 ]

Organism-specific databases

CTDi 5890.
MGIi MGI:1099436. Rad51b.

Phylogenomic databases

eggNOGi COG0468.
GeneTreei ENSGT00750000117586.
HOGENOMi HOG000154062.
HOVERGENi HBG061476.
InParanoidi O35719.
KOi K10869.
OMAi CIMMSVL.
OrthoDBi EOG70PBZC.
PhylomeDBi O35719.
TreeFami TF101219.

Enzyme and pathway databases

Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

NextBioi 296429.
PROi O35719.
SOURCEi Search...

Gene expression databases

ArrayExpressi O35719.
Bgeei O35719.
CleanExi MM_RAD51L1.
Genevestigatori O35719.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR013632. DNA_recomb/repair_Rad51_C.
IPR016467. DNA_recomb/repair_RecA-like.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
[Graphical view ]
Pfami PF08423. Rad51. 1 hit.
[Graphical view ]
PIRSFi PIRSF005856. Rad51. 1 hit.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS50162. RECA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis."
    Rice M.C., Smith S.T., Bullrich F., Havre P., Kmiec E.B.
    Proc. Natl. Acad. Sci. U.S.A. 94:7417-7422(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Mesonephros.

Entry informationi

Entry nameiRA51B_MOUSE
AccessioniPrimary (citable) accession number: O35719
Secondary accession number(s): Q8BSH6, Q9D555
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 7, 2003
Last modified: September 3, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi