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O35719

- RA51B_MOUSE

UniProt

O35719 - RA51B_MOUSE

Protein

DNA repair protein RAD51 homolog 2

Gene

Rad51b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (07 Nov 2003)
      Previous versions | rss
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    Functioni

    Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi108 – 1158ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA-dependent ATPase activity Source: InterPro
    3. double-stranded DNA binding Source: UniProtKB
    4. single-stranded DNA binding Source: UniProtKB

    GO - Biological processi

    1. double-strand break repair via homologous recombination Source: UniProtKB
    2. positive regulation of G2/M transition of mitotic cell cycle Source: UniProtKB

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein RAD51 homolog 2
    Short name:
    R51H2
    Alternative name(s):
    RAD51 homolog B
    RAD51-like protein 1
    Gene namesi
    Name:Rad51b
    Synonyms:Rad51l1, Rec2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1099436. Rad51b.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. Rad51B-Rad51C-Rad51D-XRCC2 complex Source: UniProtKB
    2. replication fork Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 350350DNA repair protein RAD51 homolog 2PRO_0000122940Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO35719.

    PTM databases

    PhosphoSiteiO35719.

    Expressioni

    Tissue specificityi

    Expressed in a wide range of tissues.

    Gene expression databases

    ArrayExpressiO35719.
    BgeeiO35719.
    CleanExiMM_RAD51L1.
    GenevestigatoriO35719.

    Interactioni

    Subunit structurei

    Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. The BCDX2 subcomplex RAD51B:RAD51C interacts with RAD51. Interacts with SWSAP1; involved in homologous recombination repair By similarity.By similarity

    Protein-protein interaction databases

    BioGridi202566. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO35719.
    SMRiO35719. Positions 10-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7575Interaction with RAD51CBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RecA family. RAD51 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0468.
    GeneTreeiENSGT00750000117586.
    HOGENOMiHOG000154062.
    HOVERGENiHBG061476.
    InParanoidiO35719.
    KOiK10869.
    OMAiCIMMSVL.
    OrthoDBiEOG70PBZC.
    PhylomeDBiO35719.
    TreeFamiTF101219.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR013632. DNA_recomb/repair_Rad51_C.
    IPR016467. DNA_recomb/repair_RecA-like.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    [Graphical view]
    PfamiPF08423. Rad51. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005856. Rad51. 1 hit.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS50162. RECA_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O35719-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSSKKLRRVG LSPELCDRLS RYQIVNCQHF LSLSPLELMK VTGLSYRGVH    50
    ELLHTVSKAC APQMQTAYEL KTRRSAHLSP AFLSTTLCAL DEALHGGVPC 100
    GSLTEITGPP GCGKTQFCIM MSVLATLPTS LGGLEGAVVY IDTESAFTAE 150
    RLVEIAESRF PQYFNTEEKL LLTSSRVHLC RELTCEGLLQ RLESLEEEII 200
    SKGVKLVIVD SIASVVRKEF DPKLQGNIKE RNKFLGKGAS LLKYLAGEFS 250
    IPVILTNQIT THLSGALPSQ ADLVSPADDL SLSEGTSGSS CLVAALGNTW 300
    GHCVNTRLIL QYLDSERRQI LIAKSPLAAF TSFVYTIKGE GLVLQGHERP 350
    Length:350
    Mass (Da):38,152
    Last modified:November 7, 2003 - v2
    Checksum:i2DB79F8D60C0529D
    GO
    Isoform 2 (identifier: O35719-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         254-258: ILTNQ → TFQPI
         259-350: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:258
    Mass (Da):28,421
    Checksum:i94C2067795B15B37
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231Q → L in AAB63359. (PubMed:9207106)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei254 – 2585ILTNQ → TFQPI in isoform 2. 1 PublicationVSP_008869
    Alternative sequencei259 – 35092Missing in isoform 2. 1 PublicationVSP_008870Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92068 mRNA. Translation: AAB63359.1.
    AK015771 mRNA. Translation: BAB29970.1.
    AK032913 mRNA. Translation: BAC28084.1.
    CCDSiCCDS56845.1. [O35719-2]
    RefSeqiNP_001239491.1. NM_001252562.1. [O35719-2]
    NP_033040.2. NM_009014.3. [O35719-1]
    UniGeneiMm.341756.

    Genome annotation databases

    EnsembliENSMUST00000079533; ENSMUSP00000078490; ENSMUSG00000059060. [O35719-2]
    GeneIDi19363.
    KEGGimmu:19363.
    UCSCiuc007oai.1. mouse. [O35719-1]
    uc007oaj.1. mouse. [O35719-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U92068 mRNA. Translation: AAB63359.1 .
    AK015771 mRNA. Translation: BAB29970.1 .
    AK032913 mRNA. Translation: BAC28084.1 .
    CCDSi CCDS56845.1. [O35719-2 ]
    RefSeqi NP_001239491.1. NM_001252562.1. [O35719-2 ]
    NP_033040.2. NM_009014.3. [O35719-1 ]
    UniGenei Mm.341756.

    3D structure databases

    ProteinModelPortali O35719.
    SMRi O35719. Positions 10-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202566. 1 interaction.

    PTM databases

    PhosphoSitei O35719.

    Proteomic databases

    PRIDEi O35719.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000079533 ; ENSMUSP00000078490 ; ENSMUSG00000059060 . [O35719-2 ]
    GeneIDi 19363.
    KEGGi mmu:19363.
    UCSCi uc007oai.1. mouse. [O35719-1 ]
    uc007oaj.1. mouse. [O35719-2 ]

    Organism-specific databases

    CTDi 5890.
    MGIi MGI:1099436. Rad51b.

    Phylogenomic databases

    eggNOGi COG0468.
    GeneTreei ENSGT00750000117586.
    HOGENOMi HOG000154062.
    HOVERGENi HBG061476.
    InParanoidi O35719.
    KOi K10869.
    OMAi CIMMSVL.
    OrthoDBi EOG70PBZC.
    PhylomeDBi O35719.
    TreeFami TF101219.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    NextBioi 296429.
    PROi O35719.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O35719.
    Bgeei O35719.
    CleanExi MM_RAD51L1.
    Genevestigatori O35719.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR013632. DNA_recomb/repair_Rad51_C.
    IPR016467. DNA_recomb/repair_RecA-like.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    [Graphical view ]
    Pfami PF08423. Rad51. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005856. Rad51. 1 hit.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS50162. RECA_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of human and mouse genes based on homology to REC2, a recombinational repair gene from the fungus Ustilago maydis."
      Rice M.C., Smith S.T., Bullrich F., Havre P., Kmiec E.B.
      Proc. Natl. Acad. Sci. U.S.A. 94:7417-7422(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Mesonephros.

    Entry informationi

    Entry nameiRA51B_MOUSE
    AccessioniPrimary (citable) accession number: O35719
    Secondary accession number(s): Q8BSH6, Q9D555
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: November 7, 2003
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3