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Protein

Liprin-beta-2

Gene

Ppfibp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Liprin-beta-2
Alternative name(s):
Coiled-coil-like protein 1
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 2
Short name:
PTPRF-interacting protein-binding protein 2
Gene namesi
Name:Ppfibp2
Synonyms:Cclp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:894649. Ppfibp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 882882Liprin-beta-2PRO_0000191037Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei362 – 3621PhosphoserineBy similarity
Modified residuei386 – 3861PhosphoserineCombined sources
Modified residuei502 – 5021PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35711.
MaxQBiO35711.
PaxDbiO35711.
PRIDEiO35711.

PTM databases

iPTMnetiO35711.
PhosphoSiteiO35711.

Expressioni

Tissue specificityi

Expressed widely. Strong expression in liver, kidney, intestine, heart, lung and testis. Low expression in brain and thymus.1 Publication

Developmental stagei

Found at 10.5 dpc through 16.5 dpc.1 Publication

Gene expression databases

BgeeiO35711.
ExpressionAtlasiO35711. baseline and differential.

Interactioni

Subunit structurei

Forms homodimers and heterodimers.By similarity

Protein-protein interaction databases

IntActiO35711. 1 interaction.
MINTiMINT-4100479.
STRINGi10090.ENSMUSP00000095738.

Structurei

3D structure databases

ProteinModelPortaliO35711.
SMRiO35711. Positions 198-263, 560-811.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini564 – 62865SAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini636 – 69964SAM 2PROSITE-ProRule annotationAdd
BLAST
Domaini724 – 78966SAM 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili101 – 303203Sequence analysisAdd
BLAST

Domaini

The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type beta/beta. The C-terminal, non-coiled coil regions mediate heterodimerization type beta/alpha (By similarity).By similarity

Sequence similaritiesi

Belongs to the liprin family. Liprin-beta subfamily.Curated
Contains 3 SAM (sterile alpha motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1899. Eukaryota.
ENOG410XSFF. LUCA.
GeneTreeiENSGT00760000119138.
HOGENOMiHOG000082535.
HOVERGENiHBG052331.
InParanoidiO35711.
OMAiMAEFRRG.
OrthoDBiEOG71G9T5.
PhylomeDBiO35711.
TreeFamiTF314207.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
InterProiIPR029515. Liprin.
IPR030435. PPFIBP2.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 1 hit.
PTHR12587:SF18. PTHR12587:SF18. 1 hit.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35711-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSDASHMLE AALEQMDGII AGTKTAADFS DGTCEPGLSP PSTCLNSMPV
60 70 80 90 100
LHLIEDLRLA LEMLALPQER EALLSQVPGP TATYIKEWFE DSLSQVNHHG
110 120 130 140 150
AASNETYQER LARLEGDKES LILQVSVLTD QVEAQGEKIR DLEVCLEGHQ
160 170 180 190 200
VKLNAAEEML QQELLSRTSL ETQKLDLMTE VSELKLKLVG MEKEQKEQEE
210 220 230 240 250
KQRKAEELLQ ELKHLKIKVE ELENERNQYE WELKATKAEV AQLQEQVALK
260 270 280 290 300
DAEIERLHSQ LSRSAALHSD HAERDQEIHR LKMGMETLLV ANEDKDRRIE
310 320 330 340 350
ELTGLLNKYL RVKEIVMATQ GPSERTLSIN EDEIEGSFRK WNTTNKSPEE
360 370 380 390 400
VPKQEISPRC SSPTPGPPPL PQKSLESRAQ KKLSCSLEDL RRESGDKCVD
410 420 430 440 450
GNQLSPVGEP KDSSFLAEQK YPTLPGKLSG ATPNGEAAKS PPTASLQPDS
460 470 480 490 500
SGSSQPKLNR GWSVSAPVLR DTEGGWEDIV SSASSGTESS PQSPVTPDGK
510 520 530 540 550
RSPKGIKKFW GKIRRTQSGN FNTDAPGMAE FRRGGLRATA GPRLSRTRDT
560 570 580 590 600
KGQKCDANAP FAQWSTERVC TWMEDFGLGQ YVIFARQWVT SGHTLLTATP
610 620 630 640 650
QDMEKELGIK HPLHRKKLVL AVKAINAKQE ETSALLDHIW VTRWLDDIGL
660 670 680 690 700
PQYKDQFHES RVDGRMLQYL TVNDLLFLKV TSQLHHLSIK CAIHVLHVNK
710 720 730 740 750
FNPNCLHRRP ADESNLSPSE VVQWSNHRVM EWLRSVDLAE YAPNLRGSGV
760 770 780 790 800
HGGLIILEPR FTGDTLAMLL NIPPQKTLLR RHLTTKFNAL IGPEAEQEKR
810 820 830 840 850
DKMASPAYTP LTTTAKVRPR KLGFSHFGNM RKKKFDESTD YICPMEPGDA
860 870 880
VSDSHRVYGV YRGLSPLDNH ELDGLDQVGQ IS
Length:882
Mass (Da):98,753
Last modified:June 12, 2007 - v3
Checksum:i4CD5BDF5C2F1A146
GO
Isoform 2 (identifier: O35711-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-469: Missing.

Note: No experimental confirmation available.
Show »
Length:871
Mass (Da):97,585
Checksum:i3BA0F9366EAF8680
GO
Isoform 3 (identifier: O35711-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     127-200: VLTDQVEAQG...MEKEQKEQEE → SLLPQLPQER...AKGDEYILCH
     201-882: Missing.

Note: Due to intron retention. No experimental confirmation available.
Show »
Length:200
Mass (Da):21,742
Checksum:i50F4AB6DE4C3800B
GO
Isoform 4 (identifier: O35711-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-469: Missing.
     877-882: QVGQIS → QMAPSEGTVTQIGLLSQDIHRLTTLLSQDQLLNDPPGCP

Note: No experimental confirmation available.
Show »
Length:904
Mass (Da):101,143
Checksum:i8567E70A422C1449
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41D → G in AAB61902 (PubMed:9199242).Curated
Sequence conflicti470 – 4701R → G in AAB61902 (PubMed:9199242).Curated
Sequence conflicti699 – 6991Missing in AAH03966 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei127 – 20074VLTDQ…KEQEE → SLLPQLPQERDAQCESSVGK RWEPIYLSCAAEHLPLHTSS PVGRLKLGASNEQNGPRHPG ACKSAKGDEYILCH in isoform 3. 1 PublicationVSP_009401Add
BLAST
Alternative sequencei201 – 882682Missing in isoform 3. 1 PublicationVSP_009402Add
BLAST
Alternative sequencei459 – 46911Missing in isoform 2 and isoform 4. 1 PublicationVSP_009403Add
BLAST
Alternative sequencei877 – 8826QVGQIS → QMAPSEGTVTQIGLLSQDIH RLTTLLSQDQLLNDPPGCP in isoform 4. 1 PublicationVSP_026136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79024 mRNA. Translation: AAB61902.1.
AK035364 mRNA. Translation: BAC29047.1.
BC003966 mRNA. Translation: AAH03966.1.
BC055935 mRNA. Translation: AAH55935.1.
BC100346 mRNA. Translation: AAI00347.1.
CCDSiCCDS21691.1. [O35711-1]
CCDS52356.1. [O35711-4]
RefSeqiNP_001157029.1. NM_001163557.1. [O35711-4]
NP_032931.2. NM_008905.2.
XP_011239998.1. XM_011241696.1. [O35711-2]
UniGeneiMm.2817.

Genome annotation databases

EnsembliENSMUST00000098134; ENSMUSP00000095738; ENSMUSG00000036528. [O35711-4]
GeneIDi19024.
KEGGimmu:19024.
UCSCiuc009jbd.2. mouse. [O35711-3]
uc009jbf.1. mouse. [O35711-4]
uc009jbh.2. mouse. [O35711-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U79024 mRNA. Translation: AAB61902.1.
AK035364 mRNA. Translation: BAC29047.1.
BC003966 mRNA. Translation: AAH03966.1.
BC055935 mRNA. Translation: AAH55935.1.
BC100346 mRNA. Translation: AAI00347.1.
CCDSiCCDS21691.1. [O35711-1]
CCDS52356.1. [O35711-4]
RefSeqiNP_001157029.1. NM_001163557.1. [O35711-4]
NP_032931.2. NM_008905.2.
XP_011239998.1. XM_011241696.1. [O35711-2]
UniGeneiMm.2817.

3D structure databases

ProteinModelPortaliO35711.
SMRiO35711. Positions 198-263, 560-811.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35711. 1 interaction.
MINTiMINT-4100479.
STRINGi10090.ENSMUSP00000095738.

PTM databases

iPTMnetiO35711.
PhosphoSiteiO35711.

Proteomic databases

EPDiO35711.
MaxQBiO35711.
PaxDbiO35711.
PRIDEiO35711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098134; ENSMUSP00000095738; ENSMUSG00000036528. [O35711-4]
GeneIDi19024.
KEGGimmu:19024.
UCSCiuc009jbd.2. mouse. [O35711-3]
uc009jbf.1. mouse. [O35711-4]
uc009jbh.2. mouse. [O35711-2]

Organism-specific databases

CTDi8495.
MGIiMGI:894649. Ppfibp2.

Phylogenomic databases

eggNOGiKOG1899. Eukaryota.
ENOG410XSFF. LUCA.
GeneTreeiENSGT00760000119138.
HOGENOMiHOG000082535.
HOVERGENiHBG052331.
InParanoidiO35711.
OMAiMAEFRRG.
OrthoDBiEOG71G9T5.
PhylomeDBiO35711.
TreeFamiTF314207.

Miscellaneous databases

ChiTaRSiPpfibp2. mouse.
PROiO35711.
SOURCEiSearch...

Gene expression databases

BgeeiO35711.
ExpressionAtlasiO35711. baseline and differential.

Family and domain databases

Gene3Di1.10.150.50. 2 hits.
InterProiIPR029515. Liprin.
IPR030435. PPFIBP2.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 1 hit.
PTHR12587:SF18. PTHR12587:SF18. 1 hit.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression analysis of mouse Cclp1, a new gene encoding a coiled-coil-like protein."
    Noben-Trauth K., Naggert J.K., Nishina P.M.
    Biochim. Biophys. Acta 1352:133-137(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Urinary bladder.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 336-882 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor and Oocyte.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-386, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-386 AND SER-502, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiLIPB2_MOUSE
AccessioniPrimary (citable) accession number: O35711
Secondary accession number(s): Q497W6
, Q7TMG4, Q8CBS6, Q99KX6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: June 12, 2007
Last modified: June 8, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.