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O35710 (NOCT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nocturnin

EC=3.1.13.4
Alternative name(s):
Carbon catabolite repression 4-like protein
Circadian deadenylase NOC
Gene names
Name:Ccrn4l
Synonyms:Ccr4, Noc
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Circadian deadenylase which plays an important role in post-transcriptional regulation of metabolic genes under circadian control. Degrades poly(A) tails of specific target mRNAs leading to their degradation and suppression of translation. Exerts a rhythmic post-transcriptional control of genes necessary for metabolic functions including nutrient absorption, glucose/insulin sensitivity, lipid metabolism, adipogenesis, inflammation and osteogenesis. Plays an important role in favoring adipogenesis over osteoblastogenesis and acts as a key regulator of the adipogenesis/osteogenesis balance. Promotes adipogenesis by activating PPARG transcriptional activity in a deadenylase-independent manner by facilitating its nuclear translocation. Regulates circadian expression of NOS2 in the liver and negatively regulates the circadian expression of IGF1 in the bone. Critical for proper development of early embryos. Ref.5 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Catalytic activity

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactor

Magnesium By similarity.

Subunit structure

Interacts with PPARG. Ref.8

Subcellular location

Cytoplasm. Nucleus. Cytoplasmperinuclear region Ref.8 Ref.13.

Tissue specificity

Highly expressed in the differentiated adipocyte (at protein level). Ubiquitous. Ref.2 Ref.3 Ref.6

Developmental stage

Expression is highest in oocytes, begins to decrease after fertilization by the 4-cell stage and then slightly increases up to the blastocyst stage. Ref.13

Induction

Immediate early gene (IEG) showing acute responses to several stimuli including serum shock, phorbol ester, lipopolysaccharide (LPS) and rosiglitazone, a PPARG agonist. Exhibits a high amplitude circadian rhythm with maximal levels in early evening. In constant darkness or constant light, the amplitude of the rhythm decreases. Expression is regulated by both light and food-entrained cues and by the CLOCK-ARNTL/BMAL1 heterodimer and PPARG. Up-regulated in cells undergoing adipogenesis. Ref.5 Ref.6 Ref.7 Ref.11

Disruption phenotype

Mice exhibit metabolic defects including a resistance to diet-induced obesity, decreased fat storage, changes in lipid-related gene expression profiles in the liver, and altered glucose and insulin sensitivities. Exhibit a delayed early embryo development and at 12 weeks of age show enhanced skeletal mass and increased osteoblastogenesis. Ref.8 Ref.10 Ref.13

Sequence similarities

Belongs to the CCR4/nocturin family.

Sequence caution

The sequence AAB62717.1 differs from that shown. Reason: Frameshift at position 125.

Ontologies

Keywords
   Biological processBiological rhythms
mRNA processing
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Metal-binding
RNA-binding
   Molecular functionExonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA phosphodiester bond hydrolysis, exonucleolytic

Inferred from direct assay Ref.5. Source: GOC

circadian regulation of gene expression

Inferred from mutant phenotype Ref.7. Source: UniProtKB

circadian rhythm

Inferred from expression pattern Ref.7Ref.11. Source: UniProtKB

cytoplasmic mRNA processing body assembly

Inferred from genetic interaction PubMed 18625844. Source: MGI

deadenylation-dependent decapping of nuclear-transcribed mRNA

Inferred from genetic interaction PubMed 18625844. Source: MGI

mRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

mRNA stabilization

Inferred from mutant phenotype Ref.11. Source: UniProtKB

negative regulation of gene expression

Inferred from mutant phenotype Ref.7. Source: UniProtKB

negative regulation of osteoblast differentiation

Inferred from mutant phenotype Ref.8. Source: UniProtKB

positive regulation of fat cell differentiation

Inferred from mutant phenotype Ref.8. Source: UniProtKB

regulation of circadian rhythm

Inferred from mutant phenotype Ref.11. Source: UniProtKB

regulation of embryonic development

Inferred from mutant phenotype Ref.13. Source: UniProtKB

response to extracellular stimulus

Inferred from direct assay Ref.5. Source: UniProtKB

response to lipopolysaccharide

Inferred from direct assay Ref.11. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay Ref.8Ref.13. Source: UniProtKB

cytoplasmic mRNA processing body

Inferred from direct assay PubMed 18625844. Source: MGI

nucleus

Inferred from direct assay Ref.13. Source: UniProtKB

perinuclear region of cytoplasm

Inferred from direct assay Ref.8. Source: UniProtKB

   Molecular_functionmRNA binding

Inferred from direct assay Ref.7. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

poly(A)-specific ribonuclease activity

Inferred from direct assay Ref.5. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.8. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O35710-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O35710-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Nocturnin
PRO_0000218569

Regions

Region341 – 35111Interaction with PPARG

Sites

Active site1931 By similarity
Active site3751 By similarity
Active site4121 By similarity
Metal binding1931Magnesium 1 By similarity
Metal binding3221Magnesium 2 By similarity
Metal binding3241Magnesium 2 By similarity
Metal binding4121Magnesium 2 By similarity

Natural variations

Alternative sequence1 – 6464Missing in isoform 2.
VSP_016677

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 2, 2001. Version 3.
Checksum: CB9FB55D84E13942

FASTA42948,301
        10         20         30         40         50         60 
MYQSPRRLCS ALLLRDAPGL RRTLVPGPRR TLAPPVLGSR PKSPQLQAAA ASGAARSRPR 

        70         80         90        100        110        120 
TVSSMGNGTS RLYSALAKTV NSSAAAQHPE YLVSTDPEHL EPIDPKELLE ECRAVLHTRP 

       130        140        150        160        170        180 
PRYQRDFVDL RTDCSSSHSP IRVMQWNILA QALGEGKDNF VQCPVEALKW EERKCLILEE 

       190        200        210        220        230        240 
ILAYQPDILC LQEVDHYFDT FQPLLSRLGY QGTFFPKPWS PCLDVEHNNG PDGCALFFLQ 

       250        260        270        280        290        300 
NRFKLISSTN IRLTAMTLKT NQVAIAQTLE CKESGRQFCI AVTHLKARTG WERFRSAQGC 

       310        320        330        340        350        360 
DLLQNLQNIT QGAKIPLIVC GDFNAEPTEE VYKHFASSSL NLNSAYKLLS PDGQSEPPYT 

       370        380        390        400        410        420 
TWKIRTSGEC RHTLDYIWYS RHALSVTSAL DLLTEEQIGP NRLPSFHYPS DHLSLVCDFS 


FNEEPHELF 

« Hide

Isoform 2 [UniParc].

Checksum: 762CA86E0F907EB8
Show »

FASTA36541,500

References

« Hide 'large scale' references
[1]"Characterization of two age-induced intracisternal A-particle-related transcripts in the mouse liver. Transcriptional read-through into an open reading frame with similarities to the yeast ccr4 transcription factor."
Puech A., Dupressoir A., Loireau M.-P., Mattei M.-G., Heidmann T.
J. Biol. Chem. 272:5995-6003(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: DBA/2J.
Tissue: Liver.
[2]"Characterization of a mammalian gene related to the yeast CCR4 general transcription factor and revealed by transposon insertion."
Dupressoir A., Barbot W., Loireau M.-P., Heidmann T.
J. Biol. Chem. 274:31068-31075(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Brain.
[3]"Rhythmic expression of nocturnin mRNA in multiple tissues of the mouse."
Wang Y., Osterbur D.L., Megaw P.L., Tosini G., Fukuhara C., Green C.B., Besharse J.C.
BMC Dev. Biol. 1:9-9(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CIRCADIAN EXPRESSION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Retina.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Ovary.
[5]"Immediate early response of the circadian polyA ribonuclease nocturnin to two extracellular stimuli."
Garbarino-Pico E., Niu S., Rollag M.D., Strayer C.A., Besharse J.C., Green C.B.
RNA 13:745-755(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[6]"Nocturnin: a circadian target of Pparg-induced adipogenesis."
Kawai M., Green C.B., Horowitz M., Ackert-Bicknell C., Lecka-Czernik B., Rosen C.J.
Ann. N. Y. Acad. Sci. 1192:131-138(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, TISSUE SPECIFICITY.
[7]"Nocturnin suppresses igf1 expression in bone by targeting the 3' untranslated region of igf1 mRNA."
Kawai M., Delany A.M., Green C.B., Adamo M.L., Rosen C.J.
Endocrinology 151:4861-4870(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[8]"A circadian-regulated gene, Nocturnin, promotes adipogenesis by stimulating PPAR-gamma nuclear translocation."
Kawai M., Green C.B., Lecka-Czernik B., Douris N., Gilbert M.R., Kojima S., Ackert-Bicknell C., Garg N., Horowitz M.C., Adamo M.L., Clemmons D.R., Rosen C.J.
Proc. Natl. Acad. Sci. U.S.A. 107:10508-10513(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INTERACTION WITH PPARG.
[9]"An essential role for the circadian-regulated gene nocturnin in osteogenesis: the importance of local timekeeping in skeletal homeostasis."
Guntur A.R., Kawai M., Le P., Bouxsein M.L., Bornstein S., Green C.B., Rosen C.J.
Ann. N. Y. Acad. Sci. 1237:58-63(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Nocturnin regulates circadian trafficking of dietary lipid in intestinal enterocytes."
Douris N., Kojima S., Pan X., Lerch-Gaggl A.F., Duong S.Q., Hussain M.M., Green C.B.
Curr. Biol. 21:1347-1355(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]"The circadian deadenylase Nocturnin is necessary for stabilization of the iNOS mRNA in mice."
Niu S., Shingle D.L., Garbarino-Pico E., Kojima S., Gilbert M., Green C.B.
PLoS ONE 6:E26954-E26954(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[12]"The role of nocturnin in early adipogenesis and modulation of systemic insulin resistance in human."
Hee S.W., Tsai S.H., Chang Y.C., Chang C.J., Yu I.S., Lee P.C., Lee W.J., Yun-Chia Chang E., Chuang L.M.
Obesity 20:1558-1565(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Functional analysis of nocturnin, a circadian deadenylase, at maternal-to-zygotic transition in mice."
Nishikawa S., Hatanaka Y., Tokoro M., Shin S.W., Shimizu N., Nishihara T., Kato R., Takemoto A., Amano T., Anzai M., Kishigami S., Hosoi Y., Matsumoto K.
J. Reprod. Dev. 59:258-265(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U70139 mRNA. Translation: AAB62717.1. Frameshift.
AF183960 mRNA. Translation: AAD56547.1.
AF199491 mRNA. Translation: AAG01384.1.
AK087790 mRNA. Translation: BAC40004.1.
CCDSCCDS17336.1. [O35710-1]
RefSeqNP_033964.1. NM_009834.2. [O35710-1]
XP_006501020.1. XM_006500957.1. [O35710-2]
UniGeneMm.86541.

3D structure databases

ProteinModelPortalO35710.
SMRO35710. Positions 120-422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-60436N.

PTM databases

PhosphoSiteO35710.

Proteomic databases

PaxDbO35710.
PRIDEO35710.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023849; ENSMUSP00000023849; ENSMUSG00000023087. [O35710-1]
ENSMUST00000167780; ENSMUSP00000130347; ENSMUSG00000023087. [O35710-1]
GeneID12457.
KEGGmmu:12457.
UCSCuc008pdm.2. mouse. [O35710-1]

Organism-specific databases

CTD25819.
MGIMGI:109382. Ccrn4l.

Phylogenomic databases

eggNOGCOG5239.
GeneTreeENSGT00550000074364.
HOGENOMHOG000237357.
HOVERGENHBG006512.
InParanoidO35710.
OMAPEYLVSP.
OrthoDBEOG7Z95MC.
PhylomeDBO35710.
TreeFamTF323175.

Enzyme and pathway databases

BRENDA3.1.13.4. 3474.

Gene expression databases

ArrayExpressO35710.
BgeeO35710.
CleanExMM_CCR4.
GenevestigatorO35710.

Family and domain databases

Gene3D3.60.10.10. 1 hit.
InterProIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMSSF56219. SSF56219. 1 hit.
ProtoNetSearch...

Other

ChiTaRSCCRN4L. mouse.
NextBio281308.
PROO35710.
SOURCESearch...

Entry information

Entry nameNOCT_MOUSE
AccessionPrimary (citable) accession number: O35710
Secondary accession number(s): Q9QZG9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 2, 2001
Last modified: July 9, 2014
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot