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Protein

Nocturnin

Gene

Noct

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Circadian deadenylase which plays an important role in post-transcriptional regulation of metabolic genes under circadian control. Degrades poly(A) tails of specific target mRNAs leading to their degradation and suppression of translation. Exerts a rhythmic post-transcriptional control of genes necessary for metabolic functions including nutrient absorption, glucose/insulin sensitivity, lipid metabolism, adipogenesis, inflammation and osteogenesis. Plays an important role in favoring adipogenesis over osteoblastogenesis and acts as a key regulator of the adipogenesis/osteogenesis balance. Promotes adipogenesis by activating PPARG transcriptional activity in a deadenylase-independent manner by facilitating its nuclear translocation. Regulates circadian expression of NOS2 in the liver and negatively regulates the circadian expression of IGF1 in the bone. Critical for proper development of early embryos.8 Publications

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei193By similarity1
Metal bindingi193Magnesium 1By similarity1
Metal bindingi322Magnesium 2By similarity1
Metal bindingi324Magnesium 2By similarity1
Active sitei375By similarity1
Active sitei412By similarity1
Metal bindingi412Magnesium 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • mRNA binding Source: UniProtKB
  • poly(A)-specific ribonuclease activity Source: UniProtKB

GO - Biological processi

  • circadian regulation of gene expression Source: UniProtKB
  • circadian rhythm Source: UniProtKB
  • cytoplasmic mRNA processing body assembly Source: MGI
  • deadenylation-dependent decapping of nuclear-transcribed mRNA Source: MGI
  • mRNA processing Source: UniProtKB-KW
  • mRNA stabilization Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of osteoblast differentiation Source: UniProtKB
  • positive regulation of fat cell differentiation Source: UniProtKB
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of embryonic development Source: UniProtKB
  • response to extracellular stimulus Source: UniProtKB
  • response to lipopolysaccharide Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Biological rhythms, mRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BRENDAi3.1.13.4. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
NocturninBy similarityImported (EC:3.1.13.4)
Alternative name(s):
Carbon catabolite repression 4-like protein
Circadian deadenylase NOC
Gene namesi
Name:NoctImported
Synonyms:Ccr4, Ccrn4l, Noc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109382. Noct.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic mRNA processing body Source: MGI
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit metabolic defects including a resistance to diet-induced obesity, decreased fat storage, changes in lipid-related gene expression profiles in the liver, and altered glucose and insulin sensitivities. Exhibit a delayed early embryo development and at 12 weeks of age show enhanced skeletal mass and increased osteoblastogenesis.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002185691 – 429NocturninAdd BLAST429

Proteomic databases

MaxQBiO35710.
PaxDbiO35710.
PRIDEiO35710.

PTM databases

iPTMnetiO35710.
PhosphoSitePlusiO35710.

Expressioni

Tissue specificityi

Highly expressed in the differentiated adipocyte (at protein level). Ubiquitous.3 Publications

Developmental stagei

Expression is highest in oocytes, begins to decrease after fertilization by the 4-cell stage and then slightly increases up to the blastocyst stage.1 Publication

Inductioni

Immediate early gene (IEG) showing acute responses to several stimuli including serum shock, phorbol ester, lipopolysaccharide (LPS) and rosiglitazone, a PPARG agonist. Exhibits a high amplitude circadian rhythm with maximal levels in early evening. In constant darkness or constant light, the amplitude of the rhythm decreases. Expression is regulated by both light and food-entrained cues and by the CLOCK-ARNTL/BMAL1 heterodimer and PPARG. Up-regulated in cells undergoing adipogenesis.4 Publications

Gene expression databases

BgeeiENSMUSG00000023087.
CleanExiMM_CCR4.
ExpressionAtlasiO35710. baseline and differential.
GenevisibleiO35710. MM.

Interactioni

Subunit structurei

Interacts with PPARG.1 Publication

Protein-protein interaction databases

DIPiDIP-60436N.
STRINGi10090.ENSMUSP00000023849.

Structurei

3D structure databases

ProteinModelPortaliO35710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni341 – 351Interaction with PPARG1 PublicationAdd BLAST11

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000237357.
HOVERGENiHBG006512.
InParanoidiO35710.
KOiK18764.
OMAiCLDVEQN.
OrthoDBiEOG091G0BF8.
PhylomeDBiO35710.
TreeFamiTF323175.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35710-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYQSPRRLCS ALLLRDAPGL RRTLVPGPRR TLAPPVLGSR PKSPQLQAAA
60 70 80 90 100
ASGAARSRPR TVSSMGNGTS RLYSALAKTV NSSAAAQHPE YLVSTDPEHL
110 120 130 140 150
EPIDPKELLE ECRAVLHTRP PRYQRDFVDL RTDCSSSHSP IRVMQWNILA
160 170 180 190 200
QALGEGKDNF VQCPVEALKW EERKCLILEE ILAYQPDILC LQEVDHYFDT
210 220 230 240 250
FQPLLSRLGY QGTFFPKPWS PCLDVEHNNG PDGCALFFLQ NRFKLISSTN
260 270 280 290 300
IRLTAMTLKT NQVAIAQTLE CKESGRQFCI AVTHLKARTG WERFRSAQGC
310 320 330 340 350
DLLQNLQNIT QGAKIPLIVC GDFNAEPTEE VYKHFASSSL NLNSAYKLLS
360 370 380 390 400
PDGQSEPPYT TWKIRTSGEC RHTLDYIWYS RHALSVTSAL DLLTEEQIGP
410 420
NRLPSFHYPS DHLSLVCDFS FNEEPHELF
Length:429
Mass (Da):48,301
Last modified:November 2, 2001 - v3
Checksum:iCB9FB55D84E13942
GO
Isoform 2 (identifier: O35710-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-64: Missing.

Note: No experimental confirmation available.
Show »
Length:365
Mass (Da):41,500
Checksum:i762CA86E0F907EB8
GO

Sequence cautioni

The sequence AAB62717 differs from that shown. Reason: Frameshift at position 125.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0166771 – 64Missing in isoform 2. 1 PublicationAdd BLAST64

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70139 mRNA. Translation: AAB62717.1. Frameshift.
AF183960 mRNA. Translation: AAD56547.1.
AF199491 mRNA. Translation: AAG01384.1.
AK087790 mRNA. Translation: BAC40004.1.
CCDSiCCDS17336.1. [O35710-1]
RefSeqiNP_033964.1. NM_009834.2. [O35710-1]
UniGeneiMm.86541.

Genome annotation databases

EnsembliENSMUST00000023849; ENSMUSP00000023849; ENSMUSG00000023087. [O35710-1]
ENSMUST00000144826; ENSMUSP00000141416; ENSMUSG00000023087. [O35710-2]
ENSMUST00000167780; ENSMUSP00000130347; ENSMUSG00000023087. [O35710-1]
GeneIDi12457.
KEGGimmu:12457.
UCSCiuc008pdm.2. mouse. [O35710-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70139 mRNA. Translation: AAB62717.1. Frameshift.
AF183960 mRNA. Translation: AAD56547.1.
AF199491 mRNA. Translation: AAG01384.1.
AK087790 mRNA. Translation: BAC40004.1.
CCDSiCCDS17336.1. [O35710-1]
RefSeqiNP_033964.1. NM_009834.2. [O35710-1]
UniGeneiMm.86541.

3D structure databases

ProteinModelPortaliO35710.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60436N.
STRINGi10090.ENSMUSP00000023849.

PTM databases

iPTMnetiO35710.
PhosphoSitePlusiO35710.

Proteomic databases

MaxQBiO35710.
PaxDbiO35710.
PRIDEiO35710.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023849; ENSMUSP00000023849; ENSMUSG00000023087. [O35710-1]
ENSMUST00000144826; ENSMUSP00000141416; ENSMUSG00000023087. [O35710-2]
ENSMUST00000167780; ENSMUSP00000130347; ENSMUSG00000023087. [O35710-1]
GeneIDi12457.
KEGGimmu:12457.
UCSCiuc008pdm.2. mouse. [O35710-1]

Organism-specific databases

CTDi25819.
MGIiMGI:109382. Noct.

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000237357.
HOVERGENiHBG006512.
InParanoidiO35710.
KOiK18764.
OMAiCLDVEQN.
OrthoDBiEOG091G0BF8.
PhylomeDBiO35710.
TreeFamiTF323175.

Enzyme and pathway databases

BRENDAi3.1.13.4. 3474.

Miscellaneous databases

ChiTaRSiCcrn4l. mouse.
PROiO35710.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023087.
CleanExiMM_CCR4.
ExpressionAtlasiO35710. baseline and differential.
GenevisibleiO35710. MM.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNOCT_MOUSE
AccessioniPrimary (citable) accession number: O35710
Secondary accession number(s): Q9QZG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 2, 2001
Last modified: November 2, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.