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Protein

Ectoderm-neural cortex protein 1

Gene

Enc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Actin-binding protein involved in the regulation of neuronal process formation and in differentiation of neural crest cells. Down-regulates transcription factor NF2L2/NRF2 by decreasing the rate of protein synthesis and not via a ubiquitin-mediated proteasomal degradation mechanism (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ectoderm-neural cortex protein 1
Short name:
ENC-1
Gene namesi
Name:Enc1
Synonyms:Enc-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:109610. Enc1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 589589Ectoderm-neural cortex protein 1PRO_0000119069Add
BLAST

Post-translational modificationi

Ubiquitinated by E3 ubiquitin ligase complex formed by CUL3 and RBX1 and probably targeted for proteasome-independent degradation. Quinone-induced oxidative stress increases its ubiquitination (By similarity).By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiO35709.
PaxDbiO35709.
PRIDEiO35709.

PTM databases

iPTMnetiO35709.
PhosphoSiteiO35709.

Expressioni

Tissue specificityi

Primarily expressed in the nervous system.

Developmental stagei

Expression is highly dynamic but mostly restricted to the nervous system. Outside the nervous system, expression is detected in the rostral-most somitomere of the presomitic mesoderm, at the times corresponding to the epithelialization that precedes somite formation. First detected in the brain and spinal chord of 12 PC embryos.

Gene expression databases

BgeeiO35709.
CleanExiMM_ENC1.
GenevisibleiO35709. MM.

Interactioni

Subunit structurei

Binds to RB1. Hypophosphorylated RB1 associates with ENC1 during neuronal differentiation, while hyperphosphorylated RB1 associates with ENC1 in undifferentiating cells. Part of a complex that contains CUL3, RBX1 and ENC1 (By similarity). Interacts indirectly with KEAP1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi199448. 1 interaction.
STRINGi10090.ENSMUSP00000038783.

Structurei

3D structure databases

ProteinModelPortaliO35709.
SMRiO35709. Positions 23-270, 277-570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 11469BTBPROSITE-ProRule annotationAdd
BLAST
Repeati296 – 34045Kelch 1Add
BLAST
Repeati341 – 38848Kelch 2Add
BLAST
Repeati389 – 44456Kelch 3Add
BLAST
Repeati446 – 49247Kelch 4Add
BLAST
Repeati494 – 53845Kelch 5Add
BLAST
Repeati539 – 58547Kelch 6Add
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00840000129689.
HOGENOMiHOG000232171.
HOVERGENiHBG000905.
InParanoidiO35709.
KOiK10462.
OMAiLDAWNSI.
OrthoDBiEOG7FJGZZ.
TreeFamiTF329218.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR030562. ENC1.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24410:SF5. PTHR24410:SF5. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 4 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35709-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSVHENRK SRASSGSINI YLFHKSSYAD SVLTHLNLLR QQRLFTDVLL
60 70 80 90 100
HAGNRTFPCH RAVLAACSRY FEAMFSGGLK ESQDSEVNFD NSIHPEVLEL
110 120 130 140 150
LLDYAYSSRV IINEENAESL LEAGDMLEFQ DIRDACAEFL EKNLHPTNCL
160 170 180 190 200
GMLLLSDAHQ CTKLYELSWR MCLSNFQTIR KNEDFLQLPQ DMVVQLLSSE
210 220 230 240 250
ELETEDERLV YESAMNWISY DLKKRYCYLP ELLQTVRLAL LPAIYLMENV
260 270 280 290 300
AMEELITKQR KSKEIVEEAI RCKLKILQND GVVTSLCARP RKTGHALFLL
310 320 330 340 350
GGQTFMCDKL YLVDQKAKEI IPKADIPSPR KEFSACAIGC KVYITGGRGS
360 370 380 390 400
ENGVSKDVWV YDTLHEEWSK AAPMLVARFG HGSAELKHCL YVVGGHTAAT
410 420 430 440 450
GCLPASPSVS LKQVEQYDPT TNKWTMVAPL REGVSNAAVV SAKLKLFAFG
460 470 480 490 500
GTSVSHDKLP KVQCYDQCEN RWSVPATCPQ PWRYTAAAVL GNQIFIMGGD
510 520 530 540 550
TEFSACSAYK FNSETYQWTK VGDVTAKRMS CHAVASGNKL YVVGGYFGIQ
560 570 580
RCKTLDCYDP TLDVWNSITT VPYSLIPTAF VSTWKHLPS
Length:589
Mass (Da):66,113
Last modified:July 27, 2011 - v2
Checksum:i21EA2CA07EE66B86
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti182 – 1821N → S in AAB64206 (PubMed:9096139).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65079 mRNA. Translation: AAB64206.1.
AK049187 mRNA. Translation: BAC33597.1.
AK132173 mRNA. Translation: BAE21011.1.
CH466567 Genomic DNA. Translation: EDL00871.1.
BC049186 mRNA. Translation: AAH49186.1.
BC058098 mRNA. Translation: AAH58098.1.
CCDSiCCDS26710.1.
RefSeqiNP_031956.3. NM_007930.4.
UniGeneiMm.241073.

Genome annotation databases

EnsembliENSMUST00000041623; ENSMUSP00000038783; ENSMUSG00000041773.
GeneIDi13803.
KEGGimmu:13803.
UCSCiuc007rod.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65079 mRNA. Translation: AAB64206.1.
AK049187 mRNA. Translation: BAC33597.1.
AK132173 mRNA. Translation: BAE21011.1.
CH466567 Genomic DNA. Translation: EDL00871.1.
BC049186 mRNA. Translation: AAH49186.1.
BC058098 mRNA. Translation: AAH58098.1.
CCDSiCCDS26710.1.
RefSeqiNP_031956.3. NM_007930.4.
UniGeneiMm.241073.

3D structure databases

ProteinModelPortaliO35709.
SMRiO35709. Positions 23-270, 277-570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199448. 1 interaction.
STRINGi10090.ENSMUSP00000038783.

PTM databases

iPTMnetiO35709.
PhosphoSiteiO35709.

Proteomic databases

MaxQBiO35709.
PaxDbiO35709.
PRIDEiO35709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000041623; ENSMUSP00000038783; ENSMUSG00000041773.
GeneIDi13803.
KEGGimmu:13803.
UCSCiuc007rod.2. mouse.

Organism-specific databases

CTDi8507.
MGIiMGI:109610. Enc1.

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00840000129689.
HOGENOMiHOG000232171.
HOVERGENiHBG000905.
InParanoidiO35709.
KOiK10462.
OMAiLDAWNSI.
OrthoDBiEOG7FJGZZ.
TreeFamiTF329218.

Miscellaneous databases

ChiTaRSiEnc1. mouse.
NextBioi284572.
PROiO35709.
SOURCEiSearch...

Gene expression databases

BgeeiO35709.
CleanExiMM_ENC1.
GenevisibleiO35709. MM.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR030562. ENC1.
IPR015915. Kelch-typ_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PANTHERiPTHR24410:SF5. PTHR24410:SF5. 1 hit.
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 4 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein."
    Hernandez M.-C., Andres-Barquin P.J., Martinez S., Bulfone A., Rubenstein J.L.R., Israel M.A.
    J. Neurosci. 17:3038-3051(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss albino.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N-3.
    Tissue: Brain and Mammary tumor.

Entry informationi

Entry nameiENC1_MOUSE
AccessioniPrimary (citable) accession number: O35709
Secondary accession number(s): Q8C7V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.