Alpha-2,8-sialyltransferase 8B - Mus musculus (Mouse)

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O35696 (SIA8B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 89. History...

Clusters with 100%, 90%, 50% identity |

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Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-2,8-sialyltransferase 8B
EC=2.4.99.-

Alternative name(s):
Polysialic acid synthase
Sialyltransferase 8B
Short name=SIAT8-B
Sialyltransferase St8Sia II
Short name=ST8SiaII
Sialyltransferase X
Short name=STX

Gene names

Name:	St8sia2
Synonyms:	Siat8b, Stx

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-glycan processing Inferred from electronic annotation. Source: Compara ganglioside biosynthetic process Inferred from electronic annotation. Source: Compara
Cellular_component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Inferred from direct assay Ref.2. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

375

Alpha-2,8-sialyltransferase 8B

PRO_0000149286

Regions

Topological domain

6

Cytoplasmic Potential

Transmembrane

17

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

352

Lumenal Potential

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Disulfide bond

By similarity

Experimental info

Sequence conflict

1

L → R in CAA67965. Ref.1

Sequence conflict

1

L → R in CAA58548. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O35696 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 34F43C519A0236CF
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375

42,411

        10         20         30         40         50         60 
MQLQFRSWML AALTLLVVFL IFADISEIEE EIGNSGGRGT IRSAVNSLHS KSNRAEVVIN 

        70         80         90        100        110        120 
GSSPPAVADR SNESLKHNIQ PASSKWRHNQ TLSLRIRKQI LKFLDAEKDI SVLKGTLKPG 

       130        140        150        160        170        180 
DIIHYIFDRD STMNVSQNLY ELLPRTSPLK NKHFQTCAIV GNSGVLLNSG CGQEIDTHSF 

       190        200        210        220        230        240 
VIRCNLAPVQ EYARDVGLKT DLVTMNPSVI QRAFEDLVNA TWREKLLQRL HGLNGSILWI 

       250        260        270        280        290        300 
PAFMARGGKE RVEWVNALIL KHHVNVRTAY PSLRLLHAVR GYWLTNKVHI KRPTTGLLMY 

       310        320        330        340        350        360 
TLATRFCNQI YLYGFWPFPL DQNQNPVKYH YYDSLKYGYT SQASPHTMPL EFKALKSLHE 

       370 
QGALKLTVGQ CDGAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic structure and promoter activity of the mouse polysialic acid synthase gene (mST8Sia II). Brain-specific expression from a TATA-less GC-rich sequence." Yoshida Y., Kurosawa N., Kanematsu T., Kojima N., Tsuji S. J. Biol. Chem. 271:30167-30173(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Brain.
[2]	"Enzymatic activity of a developmentally regulated member of the sialyltransferase family (STX): evidence for alpha 2,8-sialyltransferase activity toward N-linked oligosaccharides." Kojima N., Yoshida Y., Kurosawa N., Lee Y.C., Tsuji S. FEBS Lett. 360:1-4(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

ST8Sia II

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99646 , X99647, X99648, X99651, X99649, X99650 Genomic DNA. Translation: CAA67965.1. X83562 mRNA. Translation: CAA58548.1. BC096546 mRNA. Translation: AAH96546.1. CH466543 Genomic DNA. Translation: EDL07123.1.
IPI	IPI00132999.
PIR	I48686.
RefSeq	NP_033207.2. NM_009181.2.
UniGene	Mm.4954.
3D structure databases
ProteinModelPortal	O35696.
SMR	O35696. Positions 137-315.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59528N.
STRING	10090.ENSMUSP00000026896.
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
PTM databases
PhosphoSite	O35696.
Proteomic databases
PRIDE	O35696.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026896; ENSMUSP00000026896; ENSMUSG00000025789.
GeneID	20450.
KEGG	mmu:20450.
Organism-specific databases
CTD	8128.
MGI	MGI:106020. St8sia2.
Phylogenomic databases
eggNOG	NOG324948.
GeneTree	ENSGT00550000074407.
HOGENOM	HOG000232070.
HOVERGEN	HBG056466.
InParanoid	Q4VA47.
KO	K06612.
OMA	EIDAHSF.
OrthoDB	EOG4FTW0Z.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Bgee	O35696.
Genevestigator	O35696.
GermOnline	ENSMUSG00000025789. Mus musculus.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. IPR012163. Sialyl_trans. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
PIRSF	PIRSF005557. Sialyl_trans. 1 hit.
ProtoNet	Search...
Other
NextBio	298518.
SOURCE	Search...

Entry information

Entry name

SIA8B_MOUSE

Accession

Primary (citable) accession number: O35696
Secondary accession number(s): Q4VA47

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents