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Protein

Alpha-2,8-sialyltransferase 8B

Gene

St8sia2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei185 – 1851SubstrateBy similarity
Active sitei346 – 3461Proton donor/acceptorBy similarity

GO - Molecular functioni

  • alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.8. 3474.
ReactomeiR-MMU-4085001. Sialic acid metabolism.
R-MMU-419037. NCAM1 interactions.
R-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2,8-sialyltransferase 8B (EC:2.4.99.-)
Alternative name(s):
Polysialic acid synthase
Sialyltransferase 8B
Short name:
SIAT8-B
Sialyltransferase St8Sia II
Short name:
ST8SiaII
Sialyltransferase X
Short name:
STX
Gene namesi
Name:St8sia2
Synonyms:Siat8b, Stx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106020. St8sia2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2317Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini24 – 375352LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • early endosome Source: MGI
  • integral component of Golgi membrane Source: InterPro
  • recycling endosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Alpha-2,8-sialyltransferase 8BPRO_0000149286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence analysis
Glycosylationi72 – 721N-linked (GlcNAc...)Sequence analysis
Glycosylationi89 – 891N-linked (GlcNAc...)Sequence analysis
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence analysis
Disulfide bondi157 ↔ 307By similarity
Disulfide bondi171 ↔ 371By similarity
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence analysis
Glycosylationi234 – 2341N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO35696.
PaxDbiO35696.
PRIDEiO35696.

PTM databases

iPTMnetiO35696.
PhosphoSiteiO35696.

Expressioni

Gene expression databases

BgeeiO35696.
ExpressionAtlasiO35696. baseline and differential.
GenevisibleiO35696. MM.

Interactioni

Protein-protein interaction databases

BioGridi203244. 1 interaction.
DIPiDIP-59528N.
STRINGi10090.ENSMUSP00000026896.

Structurei

3D structure databases

ProteinModelPortaliO35696.
SMRiO35696. Positions 85-371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni206 – 2083Substrate bindingBy similarity
Regioni294 – 2963Substrate bindingBy similarity
Regioni329 – 3302Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000232070.
HOVERGENiHBG056466.
InParanoidiO35696.
KOiK06612.
OMAiIHYVFDR.
OrthoDBiEOG7VQJD1.
TreeFamiTF352820.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

O35696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLQFRSWML AALTLLVVFL IFADISEIEE EIGNSGGRGT IRSAVNSLHS
60 70 80 90 100
KSNRAEVVIN GSSPPAVADR SNESLKHNIQ PASSKWRHNQ TLSLRIRKQI
110 120 130 140 150
LKFLDAEKDI SVLKGTLKPG DIIHYIFDRD STMNVSQNLY ELLPRTSPLK
160 170 180 190 200
NKHFQTCAIV GNSGVLLNSG CGQEIDTHSF VIRCNLAPVQ EYARDVGLKT
210 220 230 240 250
DLVTMNPSVI QRAFEDLVNA TWREKLLQRL HGLNGSILWI PAFMARGGKE
260 270 280 290 300
RVEWVNALIL KHHVNVRTAY PSLRLLHAVR GYWLTNKVHI KRPTTGLLMY
310 320 330 340 350
TLATRFCNQI YLYGFWPFPL DQNQNPVKYH YYDSLKYGYT SQASPHTMPL
360 370
EFKALKSLHE QGALKLTVGQ CDGAT
Length:375
Mass (Da):42,411
Last modified:July 27, 2011 - v2
Checksum:i34F43C519A0236CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti186 – 1861L → R in CAA67965 (PubMed:8939967).Curated
Sequence conflicti186 – 1861L → R in CAA58548 (PubMed:8939967).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99646
, X99647, X99648, X99651, X99649, X99650 Genomic DNA. Translation: CAA67965.1.
X83562 mRNA. Translation: CAA58548.1.
BC096546 mRNA. Translation: AAH96546.1.
CH466543 Genomic DNA. Translation: EDL07123.1.
CCDSiCCDS21365.1.
PIRiI48686.
RefSeqiNP_033207.2. NM_009181.2.
UniGeneiMm.4954.

Genome annotation databases

EnsembliENSMUST00000026896; ENSMUSP00000026896; ENSMUSG00000025789.
GeneIDi20450.
KEGGimmu:20450.
UCSCiuc009hwe.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST8Sia II

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99646
, X99647, X99648, X99651, X99649, X99650 Genomic DNA. Translation: CAA67965.1.
X83562 mRNA. Translation: CAA58548.1.
BC096546 mRNA. Translation: AAH96546.1.
CH466543 Genomic DNA. Translation: EDL07123.1.
CCDSiCCDS21365.1.
PIRiI48686.
RefSeqiNP_033207.2. NM_009181.2.
UniGeneiMm.4954.

3D structure databases

ProteinModelPortaliO35696.
SMRiO35696. Positions 85-371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203244. 1 interaction.
DIPiDIP-59528N.
STRINGi10090.ENSMUSP00000026896.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiO35696.
PhosphoSiteiO35696.

Proteomic databases

MaxQBiO35696.
PaxDbiO35696.
PRIDEiO35696.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026896; ENSMUSP00000026896; ENSMUSG00000025789.
GeneIDi20450.
KEGGimmu:20450.
UCSCiuc009hwe.2. mouse.

Organism-specific databases

CTDi8128.
MGIiMGI:106020. St8sia2.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074407.
HOGENOMiHOG000232070.
HOVERGENiHBG056466.
InParanoidiO35696.
KOiK06612.
OMAiIHYVFDR.
OrthoDBiEOG7VQJD1.
TreeFamiTF352820.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.8. 3474.
ReactomeiR-MMU-4085001. Sialic acid metabolism.
R-MMU-419037. NCAM1 interactions.
R-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

PROiO35696.
SOURCEiSearch...

Gene expression databases

BgeeiO35696.
ExpressionAtlasiO35696. baseline and differential.
GenevisibleiO35696. MM.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic structure and promoter activity of the mouse polysialic acid synthase gene (mST8Sia II). Brain-specific expression from a TATA-less GC-rich sequence."
    Yoshida Y., Kurosawa N., Kanematsu T., Kojima N., Tsuji S.
    J. Biol. Chem. 271:30167-30173(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Tissue: Brain.
  2. "Enzymatic activity of a developmentally regulated member of the sialyltransferase family (STX): evidence for alpha 2,8-sialyltransferase activity toward N-linked oligosaccharides."
    Kojima N., Yoshida Y., Kurosawa N., Lee Y.C., Tsuji S.
    FEBS Lett. 360:1-4(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiSIA8B_MOUSE
AccessioniPrimary (citable) accession number: O35696
Secondary accession number(s): Q4VA47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.