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Protein

Pinin

Gene

Pnn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pinin
Gene namesi
Name:Pnn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1100514. Pnn.

Subcellular locationi

  • Nucleus speckle By similarity
  • Cell junctiondesmosome By similarity

  • Note: Cell-cell contact area, predominantly desmosome of intercellular adherens junction. Not a nucleocytoplasmic shuttling protein (By similarity).By similarity

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: MGI
  • cell-cell junction Source: GO_Central
  • cytoplasm Source: MGI
  • desmosome Source: MGI
  • exon-exon junction complex Source: GO_Central
  • membrane Source: MGI
  • nuclear speck Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001902432 – 725PininAdd BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei48PhosphoserineBy similarity1
Modified residuei54Omega-N-methylarginineCombined sources1
Modified residuei58PhosphoserineBy similarity1
Modified residuei66PhosphoserineCombined sources1
Modified residuei96PhosphoserineBy similarity1
Modified residuei100PhosphoserineBy similarity1
Cross-linki109Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei114PhosphoserineBy similarity1
Modified residuei115PhosphoserineBy similarity1
Cross-linki156Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki156Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei237N6-acetyllysine; alternateBy similarity1
Modified residuei237N6-succinyllysine; alternateCombined sources1
Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei346PhosphoserineBy similarity1
Modified residuei380PhosphoserineCombined sources1
Modified residuei440PhosphoserineBy similarity1
Modified residuei442PhosphoserineCombined sources1
Cross-linki536Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki561Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei666PhosphoserineBy similarity1
Modified residuei700PhosphoserineBy similarity1
Modified residuei703PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO35691.
MaxQBiO35691.
PaxDbiO35691.
PeptideAtlasiO35691.
PRIDEiO35691.

PTM databases

iPTMnetiO35691.
PhosphoSitePlusiO35691.

Expressioni

Gene expression databases

CleanExiMM_PNN.

Interactioni

Subunit structurei

Found in a mRNA splicing-dependent exon junction complex (EJC). Found in a complex with SR proteins. Found in a mRNP complex with RNPS1. Component of the PSAP complex consisting of RNPS1, SAP18 and PNN. Interacts with PNISR, CTBP1, CTBP2, KRT8, KRT18, KRT19, PS1D/PNO40, PPIG, RNPS1, SFRS4 and SRRM2. Identified in the spliceosome C complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi202283. 3 interactors.
IntActiO35691. 1 interactor.
MINTiMINT-1854171.
STRINGi10090.ENSMUSP00000021381.

Structurei

3D structure databases

ProteinModelPortaliO35691.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 283Sufficient for PSAP complex assemblyBy similarityAdd BLAST64

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili2 – 32Sequence analysisAdd BLAST31
Coiled coili162 – 233Sequence analysisAdd BLAST72
Coiled coili286 – 373Sequence analysisAdd BLAST88
Coiled coili444 – 467Sequence analysisAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi171 – 473Glu-richAdd BLAST303
Compositional biasi468 – 522Pro-richAdd BLAST55
Compositional biasi471 – 528Gln-richAdd BLAST58
Compositional biasi579 – 712Ser-richAdd BLAST134
Compositional biasi640 – 725Arg-richAdd BLAST86

Sequence similaritiesi

Belongs to the pinin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3756. Eukaryota.
ENOG4110W63. LUCA.
HOVERGENiHBG053104.
InParanoidiO35691.
KOiK13114.
PhylomeDBiO35691.

Family and domain databases

InterProiIPR006786. Pinin_SDK_MemA.
IPR006787. Pinin_SDK_N.
[Graphical view]
PfamiPF04696. Pinin_SDK_memA. 1 hit.
PF04697. Pinin_SDK_N. 1 hit.
[Graphical view]
ProDomiPD011048. Pinin_SDK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35691-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVAVRALQE QLEKAKESLK NVDENIRKLT GRDPNDVRPI QARLLALSGP
60 70 80 90 100
GGGRGRGSLL LRRGFSDSGG GPPAKQRDLE GAVSRLGGER RTRRESRQES
110 120 130 140 150
DPEDDDVKKP ALQSSVVATS KERTRDLIQD QNMDEKGKQR NRRIFGLLMG
160 170 180 190 200
TLQKFKQEST VATERQKRRQ EIEQKLEVQA EEERKQVENE RRELFEERRA
210 220 230 240 250
KQTELRLLEQ KVELAQLQEE WNEHNAKIIK YIRTKTKPHL FYIPGRMCPA
260 270 280 290 300
TQKLIEESQR KMNALFEGRR IEFAEQINKM EARPRRQSMK EKEHQVVRNE
310 320 330 340 350
EQKAEQEEGK VAQREEELEE TGNQHNDVEV EEAGEEEEKE AGIVHSDAEK
360 370 380 390 400
EQEEEEQKQE MEVKTEEEAE VREGEKQQDS QPEEVMDVLE MVESVKHVIA
410 420 430 440 450
EQEVMETNQV ESIEPSENET SKELEPEMEF DVEPDKECKS LSPGKENINS
460 470 480 490 500
QEVEKESEEK EEKEEKEPEP QPEPVAQPQP PPQPLPQSQP HSQPHSQPQP
510 520 530 540 550
VLQSQPLCQP ETLPLAVLQP PPQVIQEQGN LLPERKDFPL ESIKLPEVSV
560 570 580 590 600
EPVLTVHSEN KSKNKTRSRS RGRARNKTSK SRSRSSSSSS SSSSSTSSSS
610 620 630 640 650
GSSSSSGSSS SRSSSSSSSS TSGSSSRDSS SSTSSSSESR SRSRGRGHNR
660 670 680 690 700
DRKHRRSMDR KRRDTSGLER SHKSSKGGSS RDRKGSKDKS SRPDRKRSIS
710 720
ESSRSGKRSS RSERDRKSDR KDKRR
Length:725
Mass (Da):82,436
Last modified:January 23, 2007 - v4
Checksum:iDB99FF2DB17AAF5E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31G → A in AAN05017 (Ref. 2) Curated1
Sequence conflicti42 – 43AR → GT in CAA69960 (PubMed:9332356).Curated2
Sequence conflicti125R → RR in BAC27200 (PubMed:16141072).Curated1
Sequence conflicti216 – 217QL → HV in AAN05017 (Ref. 2) Curated2
Sequence conflicti287Q → P in CAA69960 (PubMed:9332356).Curated1
Sequence conflicti327D → H in AAN05017 (Ref. 2) Curated1
Sequence conflicti365T → I in CAA69960 (PubMed:9332356).Curated1
Sequence conflicti658M → V in BAC27200 (PubMed:16141072).Curated1
Sequence conflicti683R → T in BAC27200 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08701 mRNA. Translation: CAA69960.1.
AY129403 Genomic DNA. Translation: AAN05017.1.
AK030978 mRNA. Translation: BAC27200.1.
RefSeqiNP_032917.2. NM_008891.2.
UniGeneiMm.22347.

Genome annotation databases

GeneIDi18949.
KEGGimmu:18949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08701 mRNA. Translation: CAA69960.1.
AY129403 Genomic DNA. Translation: AAN05017.1.
AK030978 mRNA. Translation: BAC27200.1.
RefSeqiNP_032917.2. NM_008891.2.
UniGeneiMm.22347.

3D structure databases

ProteinModelPortaliO35691.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202283. 3 interactors.
IntActiO35691. 1 interactor.
MINTiMINT-1854171.
STRINGi10090.ENSMUSP00000021381.

PTM databases

iPTMnetiO35691.
PhosphoSitePlusiO35691.

Proteomic databases

EPDiO35691.
MaxQBiO35691.
PaxDbiO35691.
PeptideAtlasiO35691.
PRIDEiO35691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18949.
KEGGimmu:18949.

Organism-specific databases

CTDi5411.
MGIiMGI:1100514. Pnn.

Phylogenomic databases

eggNOGiKOG3756. Eukaryota.
ENOG4110W63. LUCA.
HOVERGENiHBG053104.
InParanoidiO35691.
KOiK13114.
PhylomeDBiO35691.

Miscellaneous databases

ChiTaRSiPnn. mouse.
PROiO35691.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PNN.

Family and domain databases

InterProiIPR006786. Pinin_SDK_MemA.
IPR006787. Pinin_SDK_N.
[Graphical view]
PfamiPF04696. Pinin_SDK_memA. 1 hit.
PF04697. Pinin_SDK_N. 1 hit.
[Graphical view]
ProDomiPD011048. Pinin_SDK_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiPININ_MOUSE
AccessioniPrimary (citable) accession number: O35691
Secondary accession number(s): Q8CD89, Q8CGU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 109 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.