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O35681

- SYT3_MOUSE

UniProt

O35681 - SYT3_MOUSE

Protein

Synaptotagmin-3

Gene

Syt3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (19 Jul 2003)
      Previous versions | rss
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    Functioni

    May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

    Cofactori

    Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi327 – 3271Calcium 1By similarity
    Metal bindingi327 – 3271Calcium 2By similarity
    Metal bindingi333 – 3331Calcium 1By similarity
    Metal bindingi385 – 3851Calcium 1By similarity
    Metal bindingi385 – 3851Calcium 2By similarity
    Metal bindingi386 – 3861Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi387 – 3871Calcium 1By similarity
    Metal bindingi387 – 3871Calcium 2By similarity
    Metal bindingi387 – 3871Calcium 3By similarity
    Metal bindingi390 – 3901Calcium 3By similarity
    Metal bindingi393 – 3931Calcium 2By similarity
    Metal bindingi393 – 3931Calcium 3By similarity

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct
    4. protein heterodimerization activity Source: UniProtKB
    5. protein homodimerization activity Source: BHF-UCL
    6. transporter activity Source: InterPro

    GO - Biological processi

    1. calcium ion-dependent exocytosis Source: InterPro

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptotagmin-3
    Alternative name(s):
    Synaptotagmin III
    Short name:
    SytIII
    Gene namesi
    Name:Syt3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:99665. Syt3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cytoplasmic vesicle, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 587587Synaptotagmin-3PRO_0000183946Add
    BLAST

    Proteomic databases

    PaxDbiO35681.
    PRIDEiO35681.

    PTM databases

    PhosphoSiteiO35681.

    Expressioni

    Gene expression databases

    CleanExiMM_SYT3.
    GenevestigatoriO35681.

    Interactioni

    Subunit structurei

    Homodimer. Can also form heterodimers with SYT6.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-457995,EBI-457995
    Syt10Q9R0N42EBI-457995,EBI-5239459
    Syt6Q9R0N82EBI-457995,EBI-5239378
    Syt9Q9R0N92EBI-457995,EBI-458006

    Protein-protein interaction databases

    IntActiO35681. 5 interactions.
    MINTiMINT-4136576.

    Structurei

    3D structure databases

    ProteinModelPortaliO35681.
    SMRiO35681. Positions 294-572.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5454VesicularSequence AnalysisAdd
    BLAST
    Topological domaini76 – 587512CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei55 – 7521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini298 – 399102C2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini430 – 533104C2 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The first C2 domain mediates Ca2+-dependent phospholipid binding.

    Sequence similaritiesi

    Belongs to the synaptotagmin family.Curated
    Contains 2 C2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5038.
    HOGENOMiHOG000232128.
    HOVERGENiHBG005010.
    InParanoidiO35681.
    PhylomeDBiO35681.

    Family and domain databases

    Gene3Di2.60.40.150. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR028682. SYT3.
    [Graphical view]
    PANTHERiPTHR10024:SF176. PTHR10024:SF176. 1 hit.
    PfamiPF00168. C2. 2 hits.
    [Graphical view]
    PRINTSiPR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTiSM00239. C2. 2 hits.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 2 hits.
    PROSITEiPS50004. C2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35681-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGDYEDDLC RRALILVSDL CARVRDADTN DRCQEFNELR IRGYPRGPDA    50
    DISVSLLSVI VTFCGIVLLG VSLFVSWKLC WVPWRDKGGS AVGGGPLRKD 100
    LAPGVGLAGL VGGGGHHLGA SLGGHPLLGG PHHHGHTAHH PPFAELLEPG 150
    GLGGSEPPEP SYLDMDSYPE AAVASVVAAG VKPSQTSPEL PSEGGTGSGL 200
    LLLPPSGGGL PSAQSHQQVT SLAPTTRYPA LPRPLTQQTL TTQADPSTEE 250
    RPPALPLPLP GGEEKAKLIG QIKPELYQGT GPGGRRGGGS GEAGAPCGRI 300
    SFALRYLYGS DHLVVRILQA LDLPAKDSNG FSDPYVKIYL LPDRKKKFQT 350
    KVHRKTLNPI FNETFQFSVP LAELAQRKLH FSVYDFDRFS RHDLIGQVVL 400
    DNLLELAEQP PDRPLWRDIL EGGSEKADLG ELNFSLCYLP TAGRLTVTII 450
    KASNLKAMDL TGFSDPYVKA SLISEGRRLK KRKTSIKKNT LNPTYNEALV 500
    FDVAPESVEN VGLSIAVVDY DCIGHNEVIG VCRVGPEAAD PHGREHWAEM 550
    LANPRKPVEH WHQLVEEKTL SSFTKGGKGL SEKENSE 587
    Length:587
    Mass (Da):63,254
    Last modified:July 19, 2003 - v2
    Checksum:i87EAEAADF22D5A4C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1111V → L in BAA08292. 1 PublicationCurated
    Sequence conflicti232 – 2321P → A in BAA08292. 1 PublicationCurated
    Sequence conflicti312 – 3121H → Q in BAA08292. 1 PublicationCurated
    Sequence conflicti312 – 3121H → Q in BAA19204. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45858 mRNA. Translation: BAA08292.1.
    AB000893 mRNA. Translation: BAA19204.1.
    BC051969 mRNA. Translation: AAH51969.1.
    CCDSiCCDS21205.1.
    RefSeqiNP_001107588.1. NM_001114116.1.
    NP_057872.3. NM_016663.3.
    UniGeneiMm.4824.

    Genome annotation databases

    GeneIDi20981.
    KEGGimmu:20981.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D45858 mRNA. Translation: BAA08292.1 .
    AB000893 mRNA. Translation: BAA19204.1 .
    BC051969 mRNA. Translation: AAH51969.1 .
    CCDSi CCDS21205.1.
    RefSeqi NP_001107588.1. NM_001114116.1.
    NP_057872.3. NM_016663.3.
    UniGenei Mm.4824.

    3D structure databases

    ProteinModelPortali O35681.
    SMRi O35681. Positions 294-572.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O35681. 5 interactions.
    MINTi MINT-4136576.

    PTM databases

    PhosphoSitei O35681.

    Proteomic databases

    PaxDbi O35681.
    PRIDEi O35681.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 20981.
    KEGGi mmu:20981.

    Organism-specific databases

    CTDi 84258.
    MGIi MGI:99665. Syt3.

    Phylogenomic databases

    eggNOGi COG5038.
    HOGENOMi HOG000232128.
    HOVERGENi HBG005010.
    InParanoidi O35681.
    PhylomeDBi O35681.

    Miscellaneous databases

    NextBioi 299972.
    PROi O35681.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_SYT3.
    Genevestigatori O35681.

    Family and domain databases

    Gene3Di 2.60.40.150. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR001565. Synaptotagmin.
    IPR028682. SYT3.
    [Graphical view ]
    PANTHERi PTHR10024:SF176. PTHR10024:SF176. 1 hit.
    Pfami PF00168. C2. 2 hits.
    [Graphical view ]
    PRINTSi PR00360. C2DOMAIN.
    PR00399. SYNAPTOTAGMN.
    SMARTi SM00239. C2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 2 hits.
    PROSITEi PS50004. C2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Kataoka M.
      Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Brain.
    2. "Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
      Fukuda M., Kanno E., Mikoshiba K.
      J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
      Fukuda M., Mikoshiba K.
      J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: HETERODIMERIZATION.
      Strain: ICR.
      Tissue: Cerebellum.

    Entry informationi

    Entry nameiSYT3_MOUSE
    AccessioniPrimary (citable) accession number: O35681
    Secondary accession number(s): P97791, Q80WV1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 19, 2003
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3