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Protein

Synaptotagmin-3

Gene

Syt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ca2+ sensor involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain. Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis. Plays a role in dendrite formation by melanocytes.By similarity

Cofactori

Ca2+By similarityNote: Binds 3 Ca2+ ions per subunit. The ions are bound to the C2 domains.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi327Calcium 1By similarity1
Metal bindingi327Calcium 2By similarity1
Metal bindingi333Calcium 1By similarity1
Metal bindingi385Calcium 1By similarity1
Metal bindingi385Calcium 2By similarity1
Metal bindingi386Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi387Calcium 1By similarity1
Metal bindingi387Calcium 2By similarity1
Metal bindingi387Calcium 3By similarity1
Metal bindingi390Calcium 3By similarity1
Metal bindingi393Calcium 2By similarity1
Metal bindingi393Calcium 3By similarity1

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: ParkinsonsUK-UCL
  • calcium ion binding Source: GO_Central
  • clathrin binding Source: GO_Central
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • syntaxin binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Differentiation, Exocytosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptotagmin-3
Alternative name(s):
Synaptotagmin III
Short name:
SytIII
Gene namesi
Name:Syt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99665. Syt3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54VesicularSequence analysisAdd BLAST54
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 587CytoplasmicSequence analysisAdd BLAST512

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi10C → A: Abolishes disulfide-dependent homodimerization. Abolishes disulfide-dependent homodimerization; when associated with A-21. 1 Publication1
Mutagenesisi21C → A: Abolishes disulfide-dependent homodimerization; when associated with A-10. Does not affect disulfide-dependent homodimerization; when associated with A-33. 1 Publication1
Mutagenesisi33C → A: Does not affect disulfide-dependent homodimerization; when associated with A-21. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001839461 – 587Synaptotagmin-3Add BLAST587

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei286Omega-N-methylarginineCombined sources1

Keywords - PTMi

Disulfide bond, Methylation

Proteomic databases

MaxQBiO35681.
PaxDbiO35681.
PeptideAtlasiO35681.
PRIDEiO35681.

PTM databases

iPTMnetiO35681.
PhosphoSitePlusiO35681.
SwissPalmiO35681.

Expressioni

Gene expression databases

CleanExiMM_SYT3.

Interactioni

Subunit structurei

Homodimer; disulfide-linked via the cysteine motif (PubMed:10531343). Can also form heterodimers with SYT6, SYT9 and SYT10 (PubMed:10531343, PubMed:10531344).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-457995,EBI-457995
Syt10Q9R0N42EBI-457995,EBI-5239459
Syt6Q9R0N82EBI-457995,EBI-5239378
Syt9Q9R0N92EBI-457995,EBI-458006

GO - Molecular functioni

  • clathrin binding Source: GO_Central
  • protein heterodimerization activity Source: UniProtKB
  • protein homodimerization activity Source: BHF-UCL
  • syntaxin binding Source: GO_Central

Protein-protein interaction databases

IntActiO35681. 5 interactors.
MINTiMINT-4136576.
STRINGi10090.ENSMUSP00000112432.

Structurei

3D structure databases

ProteinModelPortaliO35681.
SMRiO35681.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini298 – 399C2 1PROSITE-ProRule annotationAdd BLAST102
Domaini430 – 533C2 2PROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 34Cysteine motif1 PublicationAdd BLAST25

Domaini

The cysteine motif mediates homo- or heterodimer formation via formation of disulfide bonds.1 Publication
The first C2 domain mediates Ca2+-dependent phospholipid binding.By similarity

Sequence similaritiesi

Belongs to the synaptotagmin family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KDMB. Eukaryota.
COG5038. LUCA.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiO35681.
KOiK19903.
PhylomeDBiO35681.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028682. SYT3.
[Graphical view]
PANTHERiPTHR10024:SF176. PTHR10024:SF176. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDYEDDLC RRALILVSDL CARVRDADTN DRCQEFNELR IRGYPRGPDA
60 70 80 90 100
DISVSLLSVI VTFCGIVLLG VSLFVSWKLC WVPWRDKGGS AVGGGPLRKD
110 120 130 140 150
LAPGVGLAGL VGGGGHHLGA SLGGHPLLGG PHHHGHTAHH PPFAELLEPG
160 170 180 190 200
GLGGSEPPEP SYLDMDSYPE AAVASVVAAG VKPSQTSPEL PSEGGTGSGL
210 220 230 240 250
LLLPPSGGGL PSAQSHQQVT SLAPTTRYPA LPRPLTQQTL TTQADPSTEE
260 270 280 290 300
RPPALPLPLP GGEEKAKLIG QIKPELYQGT GPGGRRGGGS GEAGAPCGRI
310 320 330 340 350
SFALRYLYGS DHLVVRILQA LDLPAKDSNG FSDPYVKIYL LPDRKKKFQT
360 370 380 390 400
KVHRKTLNPI FNETFQFSVP LAELAQRKLH FSVYDFDRFS RHDLIGQVVL
410 420 430 440 450
DNLLELAEQP PDRPLWRDIL EGGSEKADLG ELNFSLCYLP TAGRLTVTII
460 470 480 490 500
KASNLKAMDL TGFSDPYVKA SLISEGRRLK KRKTSIKKNT LNPTYNEALV
510 520 530 540 550
FDVAPESVEN VGLSIAVVDY DCIGHNEVIG VCRVGPEAAD PHGREHWAEM
560 570 580
LANPRKPVEH WHQLVEEKTL SSFTKGGKGL SEKENSE
Length:587
Mass (Da):63,254
Last modified:July 19, 2003 - v2
Checksum:i87EAEAADF22D5A4C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti111V → L in BAA08292 (Ref. 1) Curated1
Sequence conflicti232P → A in BAA08292 (Ref. 1) Curated1
Sequence conflicti312H → Q in BAA08292 (Ref. 1) Curated1
Sequence conflicti312H → Q in BAA19204 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45858 mRNA. Translation: BAA08292.1.
AB000893 mRNA. Translation: BAA19204.1.
BC051969 mRNA. Translation: AAH51969.1.
CCDSiCCDS21205.1.
RefSeqiNP_001107588.1. NM_001114116.1.
NP_057872.3. NM_016663.3.
UniGeneiMm.4824.

Genome annotation databases

GeneIDi20981.
KEGGimmu:20981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D45858 mRNA. Translation: BAA08292.1.
AB000893 mRNA. Translation: BAA19204.1.
BC051969 mRNA. Translation: AAH51969.1.
CCDSiCCDS21205.1.
RefSeqiNP_001107588.1. NM_001114116.1.
NP_057872.3. NM_016663.3.
UniGeneiMm.4824.

3D structure databases

ProteinModelPortaliO35681.
SMRiO35681.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO35681. 5 interactors.
MINTiMINT-4136576.
STRINGi10090.ENSMUSP00000112432.

PTM databases

iPTMnetiO35681.
PhosphoSitePlusiO35681.
SwissPalmiO35681.

Proteomic databases

MaxQBiO35681.
PaxDbiO35681.
PeptideAtlasiO35681.
PRIDEiO35681.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi20981.
KEGGimmu:20981.

Organism-specific databases

CTDi84258.
MGIiMGI:99665. Syt3.

Phylogenomic databases

eggNOGiENOG410KDMB. Eukaryota.
COG5038. LUCA.
HOGENOMiHOG000232128.
HOVERGENiHBG005010.
InParanoidiO35681.
KOiK19903.
PhylomeDBiO35681.

Miscellaneous databases

PROiO35681.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SYT3.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028682. SYT3.
[Graphical view]
PANTHERiPTHR10024:SF176. PTHR10024:SF176. 1 hit.
PfamiPF00168. C2. 2 hits.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTiSM00239. C2. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYT3_MOUSE
AccessioniPrimary (citable) accession number: O35681
Secondary accession number(s): P97791, Q80WV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 19, 2003
Last modified: November 30, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.