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O35681 (SYT3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptotagmin-3
Alternative name(s):
Synaptotagmin III
Short name=SytIII
Gene names
Name:Syt3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length587 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis.

Cofactor

Binds 3 calcium ions per subunit. The ions are bound to the C2 domains By similarity.

Subunit structure

Homodimer. Can also form heterodimers with SYT6. Ref.4

Subcellular location

Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane; Single-pass membrane protein.

Domain

The first C2 domain mediates Ca2+-dependent phospholipid binding.

Sequence similarities

Belongs to the synaptotagmin family.

Contains 2 C2 domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 587587Synaptotagmin-3
PRO_0000183946

Regions

Topological domain1 – 5454Vesicular Potential
Transmembrane55 – 7521Helical; Potential
Topological domain76 – 587512Cytoplasmic Potential
Domain298 – 399102C2 1
Domain430 – 533104C2 2

Sites

Metal binding3271Calcium 1 By similarity
Metal binding3271Calcium 2 By similarity
Metal binding3331Calcium 1 By similarity
Metal binding3851Calcium 1 By similarity
Metal binding3851Calcium 2 By similarity
Metal binding3861Calcium 1; via carbonyl oxygen By similarity
Metal binding3871Calcium 1 By similarity
Metal binding3871Calcium 2 By similarity
Metal binding3871Calcium 3 By similarity
Metal binding3901Calcium 3 By similarity
Metal binding3931Calcium 2 By similarity
Metal binding3931Calcium 3 By similarity

Experimental info

Sequence conflict1111V → L in BAA08292. Ref.1
Sequence conflict2321P → A in BAA08292. Ref.1
Sequence conflict3121H → Q in BAA08292. Ref.1
Sequence conflict3121H → Q in BAA19204. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O35681 [UniParc].

Last modified July 19, 2003. Version 2.
Checksum: 87EAEAADF22D5A4C

FASTA58763,254
        10         20         30         40         50         60 
MSGDYEDDLC RRALILVSDL CARVRDADTN DRCQEFNELR IRGYPRGPDA DISVSLLSVI 

        70         80         90        100        110        120 
VTFCGIVLLG VSLFVSWKLC WVPWRDKGGS AVGGGPLRKD LAPGVGLAGL VGGGGHHLGA 

       130        140        150        160        170        180 
SLGGHPLLGG PHHHGHTAHH PPFAELLEPG GLGGSEPPEP SYLDMDSYPE AAVASVVAAG 

       190        200        210        220        230        240 
VKPSQTSPEL PSEGGTGSGL LLLPPSGGGL PSAQSHQQVT SLAPTTRYPA LPRPLTQQTL 

       250        260        270        280        290        300 
TTQADPSTEE RPPALPLPLP GGEEKAKLIG QIKPELYQGT GPGGRRGGGS GEAGAPCGRI 

       310        320        330        340        350        360 
SFALRYLYGS DHLVVRILQA LDLPAKDSNG FSDPYVKIYL LPDRKKKFQT KVHRKTLNPI 

       370        380        390        400        410        420 
FNETFQFSVP LAELAQRKLH FSVYDFDRFS RHDLIGQVVL DNLLELAEQP PDRPLWRDIL 

       430        440        450        460        470        480 
EGGSEKADLG ELNFSLCYLP TAGRLTVTII KASNLKAMDL TGFSDPYVKA SLISEGRRLK 

       490        500        510        520        530        540 
KRKTSIKKNT LNPTYNEALV FDVAPESVEN VGLSIAVVDY DCIGHNEVIG VCRVGPEAAD 

       550        560        570        580 
PHGREHWAEM LANPRKPVEH WHQLVEEKTL SSFTKGGKGL SEKENSE 

« Hide

References

« Hide 'large scale' references
[1]Kataoka M.
Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[2]"Conserved N-terminal cysteine motif is essential for homo- and heterodimer formation of synaptotagmins III, V, VI, and X."
Fukuda M., Kanno E., Mikoshiba K.
J. Biol. Chem. 274:31421-31427(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"A novel alternatively spliced variant of synaptotagmin VI lacking a transmembrane domain. Implications for distinct functions of the two isoforms."
Fukuda M., Mikoshiba K.
J. Biol. Chem. 274:31428-31434(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: HETERODIMERIZATION.
Strain: ICR.
Tissue: Cerebellum.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D45858 mRNA. Translation: BAA08292.1.
AB000893 mRNA. Translation: BAA19204.1.
BC051969 mRNA. Translation: AAH51969.1.
CCDSCCDS21205.1.
RefSeqNP_001107588.1. NM_001114116.1.
NP_057872.3. NM_016663.3.
UniGeneMm.4824.

3D structure databases

ProteinModelPortalO35681.
SMRO35681. Positions 294-572.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO35681. 5 interactions.
MINTMINT-4136576.

PTM databases

PhosphoSiteO35681.

Proteomic databases

PaxDbO35681.
PRIDEO35681.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID20981.
KEGGmmu:20981.

Organism-specific databases

CTD84258.
MGIMGI:99665. Syt3.

Phylogenomic databases

eggNOGCOG5038.
HOGENOMHOG000232128.
HOVERGENHBG005010.
InParanoidO35681.
PhylomeDBO35681.

Gene expression databases

CleanExMM_SYT3.
GenevestigatorO35681.

Family and domain databases

Gene3D2.60.40.150. 2 hits.
InterProIPR000008. C2_dom.
IPR001565. Synaptotagmin.
IPR028682. SYT3.
[Graphical view]
PANTHERPTHR10024:SF176. PTHR10024:SF176. 1 hit.
PfamPF00168. C2. 2 hits.
[Graphical view]
PRINTSPR00360. C2DOMAIN.
PR00399. SYNAPTOTAGMN.
SMARTSM00239. C2. 2 hits.
[Graphical view]
SUPFAMSSF49562. SSF49562. 2 hits.
PROSITEPS50004. C2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio299972.
PROO35681.
SOURCESearch...

Entry information

Entry nameSYT3_MOUSE
AccessionPrimary (citable) accession number: O35681
Secondary accession number(s): P97791, Q80WV1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 19, 2003
Last modified: July 9, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot