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Protein

Monoglyceride lipase

Gene

Mgll

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (PubMed:9341166, PubMed:17700715, PubMed:18096503, PubMed:19029917, PubMed:20729846). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth (By similarity).By similarity5 Publications

Catalytic activityi

Hydrolyzes glycerol monoesters of long-chain fatty acids.2 Publications

Pathwayi: triacylglycerol degradation

This protein is involved in the pathway triacylglycerol degradation, which is part of Glycerolipid metabolism.2 Publications
View all proteins of this organism that are known to be involved in the pathway triacylglycerol degradation and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei122 – 1221Nucleophile1 Publication
Active sitei239 – 2391Charge relay system1 Publication
Active sitei269 – 2691Charge relay system1 Publication

GO - Molecular functioni

  • acylglycerol lipase activity Source: UniProtKB
  • protein homodimerization activity Source: MGI

GO - Biological processi

  • acylglycerol catabolic process Source: UniProtKB
  • arachidonic acid metabolic process Source: UniProtKB
  • fatty acid biosynthetic process Source: UniProtKB-KW
  • long term synaptic depression Source: MGI
  • regulation of axon extension Source: MGI
  • regulation of endocannabinoid signaling pathway Source: UniProtKB
  • regulation of inflammatory response Source: UniProtKB
  • regulation of sensory perception of pain Source: UniProtKB
  • regulation of signal transduction Source: UniProtKB
  • triglyceride catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1482883. Acyl chain remodeling of DAG and TAG.
R-MMU-426048. Arachidonate production from DAG.
UniPathwayiUPA00256.

Protein family/group databases

ESTHERimouse-MGLL. Monoglyceridelipase_lysophospholip.
MEROPSiS33.979.

Chemistry

SwissLipidsiSLP:000000321.
SLP:000000521.

Names & Taxonomyi

Protein namesi
Recommended name:
Monoglyceride lipaseImported (EC:3.1.1.232 Publications)
Short name:
MGL
Alternative name(s):
Monoacylglycerol lipase
Short name:
MAGL
Gene namesi
Name:MgllImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1346042. Mgll.

Subcellular locationi

GO - Cellular componenti

  • axon Source: MGI
  • cytosol Source: UniProtKB
  • extrinsic component of membrane Source: UniProtKB
  • nucleoplasm Source: MGI
  • synapse Source: MGI
  • varicosity Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Ten-fold increase in brain 2-arachidonoylglycerol levels, combined with low levels of brain arachidonic acid.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi122 – 1221S → A: Abolishes activity. 1 Publication
Mutagenesisi239 – 2391D → A: Abolishes activity. 1 Publication
Mutagenesisi243 – 2431D → A: Slightly reduces activity. 1 Publication
Mutagenesisi269 – 2691H → A: Abolishes activity. 1 Publication
Mutagenesisi272 – 2721H → A: Reduces activity. 1 Publication
Mutagenesisi284 – 2841H → A: Slightly reduces activity. 1 Publication
Mutagenesisi292 – 2921H → A: Slightly reduces activity. 1 Publication

Chemistry

ChEMBLiCHEMBL5774.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Monoglyceride lipasePRO_0000191266Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101PhosphothreonineCombined sources
Modified residuei58 – 581Nitrated tyrosineCombined sources
Modified residuei189 – 1891PhosphoserineCombined sources

Keywords - PTMi

Nitration, Phosphoprotein

Proteomic databases

MaxQBiO35678.
PaxDbiO35678.
PeptideAtlasiO35678.
PRIDEiO35678.

PTM databases

iPTMnetiO35678.
PhosphoSiteiO35678.
SwissPalmiO35678.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiENSMUSG00000033174.
CleanExiMM_MGLL.
ExpressionAtlasiO35678. baseline and differential.
GenevisibleiO35678. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204809. 1 interaction.
IntActiO35678. 2 interactions.
MINTiMINT-1856023.
STRINGi10090.ENSMUSP00000127374.

Chemistry

BindingDBiO35678.

Structurei

3D structure databases

ProteinModelPortaliO35678.
SMRiO35678. Positions 6-295.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1455. Eukaryota.
COG2267. LUCA.
GeneTreeiENSGT00390000011364.
HOGENOMiHOG000214242.
HOVERGENiHBG049220.
InParanoidiO35678.
KOiK01054.
PhylomeDBiO35678.
TreeFamiTF329184.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022742. Hydrolase_4.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O35678-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEASSPRRT PQNVPYQDLP HLVNADGQYL FCRYWKPSGT PKALIFVSHG
60 70 80 90 100
AGEHCGRYDE LAHMLKGLDM LVFAHDHVGH GQSEGERMVV SDFQVFVRDV
110 120 130 140 150
LQHVDTIQKD YPDVPIFLLG HSMGGAISIL VAAERPTYFS GMVLISPLVL
160 170 180 190 200
ANPESASTLK VLAAKLLNFV LPNMTLGRID SSVLSRNKSE VDLYNSDPLV
210 220 230 240 250
CRAGLKVCFG IQLLNAVARV ERAMPRLTLP FLLLQGSADR LCDSKGAYLL
260 270 280 290 300
MESSRSQDKT LKMYEGAYHV LHRELPEVTN SVLHEVNSWV SHRIAAAGAG

CPP
Length:303
Mass (Da):33,388
Last modified:January 1, 1998 - v1
Checksum:iCB753ECDE38EBEC1
GO
Isoform 2 (identifier: O35678-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     78-303: VGHGQSEGER...IAAAGAGCPP → AVMLSAALQS...GVCCGGRCHY

Note: No experimental confirmation available.
Show »
Length:116
Mass (Da):13,043
Checksum:iDEF334F027BF1662
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei78 – 303226VGHGQ…AGCPP → AVMLSAALQSALVIFVRLIW QIIFQGYPRGVCCGGRCHY in isoform 2. 1 PublicationVSP_010315Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001118 mRNA. Translation: CAA04544.1.
AJ316580 mRNA. Translation: CAC69874.1.
AK006949 mRNA. Translation: BAB24800.1.
AK131645 mRNA. Translation: BAE20737.1.
BC057965 mRNA. Translation: AAH57965.1.
CCDSiCCDS20338.1. [O35678-1]
RefSeqiNP_001159721.1. NM_001166249.1.
NP_001159722.1. NM_001166250.1.
NP_001159723.1. NM_001166251.1.
NP_035974.1. NM_011844.4. [O35678-1]
UniGeneiMm.272197.

Genome annotation databases

EnsembliENSMUST00000089449; ENSMUSP00000086872; ENSMUSG00000033174. [O35678-1]
ENSMUST00000113585; ENSMUSP00000109215; ENSMUSG00000033174. [O35678-1]
ENSMUST00000150180; ENSMUSP00000145068; ENSMUSG00000033174. [O35678-2]
GeneIDi23945.
KEGGimmu:23945.
UCSCiuc009cvp.2. mouse. [O35678-1]
uc009cvq.2. mouse. [O35678-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001118 mRNA. Translation: CAA04544.1.
AJ316580 mRNA. Translation: CAC69874.1.
AK006949 mRNA. Translation: BAB24800.1.
AK131645 mRNA. Translation: BAE20737.1.
BC057965 mRNA. Translation: AAH57965.1.
CCDSiCCDS20338.1. [O35678-1]
RefSeqiNP_001159721.1. NM_001166249.1.
NP_001159722.1. NM_001166250.1.
NP_001159723.1. NM_001166251.1.
NP_035974.1. NM_011844.4. [O35678-1]
UniGeneiMm.272197.

3D structure databases

ProteinModelPortaliO35678.
SMRiO35678. Positions 6-295.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204809. 1 interaction.
IntActiO35678. 2 interactions.
MINTiMINT-1856023.
STRINGi10090.ENSMUSP00000127374.

Chemistry

BindingDBiO35678.
ChEMBLiCHEMBL5774.
SwissLipidsiSLP:000000321.
SLP:000000521.

Protein family/group databases

ESTHERimouse-MGLL. Monoglyceridelipase_lysophospholip.
MEROPSiS33.979.

PTM databases

iPTMnetiO35678.
PhosphoSiteiO35678.
SwissPalmiO35678.

Proteomic databases

MaxQBiO35678.
PaxDbiO35678.
PeptideAtlasiO35678.
PRIDEiO35678.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089449; ENSMUSP00000086872; ENSMUSG00000033174. [O35678-1]
ENSMUST00000113585; ENSMUSP00000109215; ENSMUSG00000033174. [O35678-1]
ENSMUST00000150180; ENSMUSP00000145068; ENSMUSG00000033174. [O35678-2]
GeneIDi23945.
KEGGimmu:23945.
UCSCiuc009cvp.2. mouse. [O35678-1]
uc009cvq.2. mouse. [O35678-2]

Organism-specific databases

CTDi11343.
MGIiMGI:1346042. Mgll.

Phylogenomic databases

eggNOGiKOG1455. Eukaryota.
COG2267. LUCA.
GeneTreeiENSGT00390000011364.
HOGENOMiHOG000214242.
HOVERGENiHBG049220.
InParanoidiO35678.
KOiK01054.
PhylomeDBiO35678.
TreeFamiTF329184.

Enzyme and pathway databases

UniPathwayiUPA00256.
ReactomeiR-MMU-1482883. Acyl chain remodeling of DAG and TAG.
R-MMU-426048. Arachidonate production from DAG.

Miscellaneous databases

ChiTaRSiMgll. mouse.
PROiO35678.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033174.
CleanExiMM_MGLL.
ExpressionAtlasiO35678. baseline and differential.
GenevisibleiO35678. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022742. Hydrolase_4.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGLL_MOUSE
AccessioniPrimary (citable) accession number: O35678
Secondary accession number(s): Q3V2R0, Q9D9G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Short-term inhibition causes analgesia, while long-term inhibition causes tolerance to endocannabinoids acting on brain cannabinoid receptor CNR1, and a reduction in brain cannabinoid receptor CNR1 activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.