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O35674 (ADA19_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Disintegrin and metalloproteinase domain-containing protein 19

Short name=ADAM 19
EC=3.4.24.-
Alternative name(s):
Meltrin-beta
Gene names
Name:Adam19
Synonyms:Mltnb
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length920 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone By similarity. Ref.4

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Interacts with SH3PXD2A By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Widely expressed, with the highest expression in bone, heart and lung, followed by brain and spleen and relatively low expression in liver, skeletal muscle, kidney and testis. In bone, primarily expressed in cell of the osteoblast lineage and not detected in mature osteoclasts.

Developmental stage

Expressed in the heart and in the tail bud at 8.0 dpc, and then in the cranial and dorsal root ganglia. Also expressed weakly and transiently in the intestine, lung and in bone marrow. Ref.2

Induction

By calcitriol and during osteoblast differentiation.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Sequence similarities

Contains 1 disintegrin domain.

Contains 1 EGF-like domain.

Contains 1 peptidase M12B domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Propeptide27 – 204178 By similarity
PRO_0000029104
Chain205 – 920716Disintegrin and metalloproteinase domain-containing protein 19
PRO_0000029105

Regions

Topological domain27 – 703677Extracellular Potential
Transmembrane704 – 72421Helical; Potential
Topological domain725 – 920196Cytoplasmic Potential
Domain211 – 409199Peptidase M12B
Domain417 – 50387Disintegrin
Domain654 – 68633EGF-like
Motif131 – 1388Cysteine switch By similarity
Motif835 – 84612SH3-binding Potential
Compositional bias435 – 4384Poly-Glu
Compositional bias504 – 653150Cys-rich
Compositional bias616 – 6216Poly-Glu

Sites

Active site3471
Metal binding1331Zinc; in inhibited form By similarity
Metal binding3461Zinc; catalytic
Metal binding3501Zinc; catalytic
Metal binding3561Zinc; catalytic

Amino acid modifications

Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation4451N-linked (GlcNAc...) Potential
Glycosylation4481N-linked (GlcNAc...) Potential
Glycosylation6491N-linked (GlcNAc...) Potential
Disulfide bond321 ↔ 404 By similarity
Disulfide bond361 ↔ 388 By similarity
Disulfide bond362 ↔ 371 By similarity
Disulfide bond475 ↔ 495 By similarity
Disulfide bond658 ↔ 668 By similarity
Disulfide bond662 ↔ 674 By similarity
Disulfide bond676 ↔ 685 By similarity

Sequences

Sequence LengthMass (Da)Tools
O35674 [UniParc].

Last modified November 1, 1998. Version 2.
Checksum: 7094FDD4EE547382

FASTA920100,860
        10         20         30         40         50         60 
MPGRAGVARF CLLALALQLH WPLAACEPGW TTRGSQEGSP PLQHELIIPQ WRTSESPGRG 

        70         80         90        100        110        120 
KHPLRAELRV MAEGRELILD LEKNEHLFAP AYTETCYTAS GNPQTSTLKS EDHCFYHGTV 

       130        140        150        160        170        180 
RDVDESSVTL STCRGIRGLI IVRSNLSYII EPVPNSDSQH RIYRSEHLTL PPGNCGFEHS 

       190        200        210        220        230        240 
GPTSKDWALQ FTHQTKKQPR RMKREDLHSM KYVELYLVAD YAEFQKNRHD QDATKRKLME 

       250        260        270        280        290        300 
IANYVDKFYR SLNIRIALVG LEVWTHGDKC EVSENPYSTL WSFLSWRRKL LAQKSHDNAQ 

       310        320        330        340        350        360 
LITGRSFQGT TIGLAPLMAM CSVYQSGGVS MDHSENAIGV ASTVAHEIGH NFGMSHDSAH 

       370        380        390        400        410        420 
CCSASAADGG CIMAAATGHP FPKVFSWCNR KELDRYLQTG GGMCLSNMPD TRTLYGGRRC 

       430        440        450        460        470        480 
GNGYLEDGEE CDCGEEEECK NPCCNASNCT LKEGAECAHG SCCHQCKLVA PGTQCREQVR 

       490        500        510        520        530        540 
QCDLPEFCTG KSPHCPTNYY QMDGTPCEGG QAYCYNGMCL TYQEQCQQLW GPGARPALDL 

       550        560        570        580        590        600 
CFERVNAAGD TYGNCGKGLN GQYRKCSPRD AKCGKIQCQS TQARPLESNA VSIDTTITLN 

       610        620        630        640        650        660 
GRRIHCRGTH VYRGPEEEEG EGDMLDPGLV MTGTKCGHNH ICFEGQCRNT SFFETEGCGK 

       670        680        690        700        710        720 
KCNGHGVCNN NKNCHCFPGW SPPFCNTPGD GGSVDSGPLP PKSVGPVIAG VFSALFVLAV 

       730        740        750        760        770        780 
LVLLCHCYRQ SHKLGKPSAL PFKLRHQFSC PFRVSQSGGT GHANPTFKLQ TPQGKRKVTN 

       790        800        810        820        830        840 
TPESLRKPSH PPPRPPPDYL RVESPPAPLP AHLNRAAGSS PEAGARIERK ESARRPPPSR 

       850        860        870        880        890        900 
PMPPAPNCLL SQDFSRPRPP QKALPANPVP GQRTGPRSGG TSLLQPPTSG PQPPRPPAVP 

       910        920 
VPKLPEYRSQ RVGAIISSKI 

« Hide

References

[1]"Cloning and initial characterization of mouse meltrin beta and analysis of the expression of four metalloprotease-disintegrins in bone cells."
Inoue D., Reid M.S., Lum L., Kraetzschmar J., Weskamp G., Myung Y.M., Baron R., Blobel C.P.
J. Biol. Chem. 273:4180-4187(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Myoblast.
[2]"Spatially- and temporally-restricted expression of meltrin alpha (ADAM12) and beta (ADAM19) in mouse embryo."
Kurisaki T., Masuda A., Osumi N., Nabeshima Y., Fujisawa-Sehara A.
Mech. Dev. 73:211-215(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
Tissue: Myoblast.
[3]"A metalloprotease-disintegrin participating in myoblast fusion."
Yagami-Hiromasa T., Sato T., Kurisaki T., Kamijo K., Nabeshima Y., Fujisawa-Sehara A.
Nature 377:652-656(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 429-578.
Tissue: Embryonic fibroblast.
[4]"Roles of Meltrin beta /ADAM19 in the processing of neuregulin."
Shirakabe K., Wakatsuki S., Kurisaki T., Fujisawa-Sehara A.
J. Biol. Chem. 276:9352-9358(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF019887 mRNA. Translation: AAC40037.1.
D50410 mRNA. Translation: BAA18923.2.
PIRPC7067.
RefSeqNP_033746.1. NM_009616.3.
UniGeneMm.89940.

3D structure databases

ProteinModelPortalO35674.
SMRO35674. Positions 206-691.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996200.

Protein family/group databases

MEROPSM12.214.

PTM databases

PhosphoSiteO35674.

Proteomic databases

PRIDEO35674.

Protocols and materials databases

DNASU11492.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000011400; ENSMUSP00000011400; ENSMUSG00000011256.
GeneID11492.
KEGGmmu:11492.
UCSCuc007iny.1. mouse.

Organism-specific databases

CTD8728.
MGIMGI:105377. Adam19.

Phylogenomic databases

eggNOGNOG294463.
HOGENOMHOG000230883.
HOVERGENHBG006978.
InParanoidO35674.
KOK08608.
OMAFQKNRRD.
OrthoDBEOG7F7W89.
PhylomeDBO35674.
TreeFamTF314733.

Gene expression databases

ArrayExpressO35674.
BgeeO35674.
GenevestigatorO35674.

Family and domain databases

Gene3D3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSPR00289. DISINTEGRIN.
SMARTSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMSSF57552. SSF57552. 1 hit.
PROSITEPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278870.
PROO35674.
SOURCESearch...

Entry information

Entry nameADA19_MOUSE
AccessionPrimary (citable) accession number: O35674
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot