Disintegrin and metalloproteinase domain-containing protein 19 precursor

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O35674 (ADA19_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 117. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Disintegrin and metalloproteinase domain-containing protein 19
Short name=ADAM 19
EC=3.4.24.-

Alternative name(s):
Meltrin-beta

Gene names

Name:	Adam19
Synonyms:	Mltnb

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	920 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone By similarity. Ref.4
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Interacts with SH3PXD2A By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Widely expressed, with the highest expression in bone, heart and lung, followed by brain and spleen and relatively low expression in liver, skeletal muscle, kidney and testis. In bone, primarily expressed in cell of the osteoblast lineage and not detected in mature osteoclasts.
Developmental stage	Expressed in the heart and in the tail bud at 8.0 dpc, and then in the cranial and dorsal root ganglia. Also expressed weakly and transiently in the intestine, lung and in bone marrow. Ref.2
Induction	By calcitriol and during osteoblast differentiation.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Contains 1 disintegrin domain. Contains 1 EGF-like domain. Contains 1 peptidase M12B domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	EGF-like domain SH3-binding Signal Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heart development Inferred from mutant phenotype PubMed 14673146 PubMed 14975714. Source: MGI membrane protein ectodomain proteolysis Inferred from mutant phenotype PubMed 14975714. Source: MGI
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

Potential

Propeptide

27 – 204

178

By similarity

PRO_0000029104

Chain

205 – 920

716

Disintegrin and metalloproteinase domain-containing protein 19

PRO_0000029105

Regions

Topological domain

27 – 703

677

Extracellular Potential

Transmembrane

704 – 724

Helical; Potential

Topological domain

725 – 920

196

Cytoplasmic Potential

Domain

211 – 409

199

Peptidase M12B

Domain

417 – 503

Disintegrin

Domain

654 – 686

EGF-like

Motif

131 – 138

Cysteine switch By similarity

Motif

835 – 846

SH3-binding Potential

Compositional bias

435 – 438

Poly-Glu

Compositional bias

504 – 653

150

Cys-rich

Compositional bias

616 – 621

Poly-Glu

Sites

Active site

347

Metal binding

133

Zinc; in inhibited form By similarity

Metal binding

346

Zinc; catalytic

Metal binding

350

Zinc; catalytic

Metal binding

356

Zinc; catalytic

Amino acid modifications

Glycosylation

145

N-linked (GlcNAc...) Potential

Glycosylation

445

N-linked (GlcNAc...) Potential

Glycosylation

448

N-linked (GlcNAc...) Potential

Glycosylation

649

N-linked (GlcNAc...) Potential

Disulfide bond

321 ↔ 404

By similarity

Disulfide bond

361 ↔ 388

By similarity

Disulfide bond

362 ↔ 371

By similarity

Disulfide bond

475 ↔ 495

By similarity

Disulfide bond

658 ↔ 668

By similarity

Disulfide bond

662 ↔ 674

By similarity

Disulfide bond

676 ↔ 685

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O35674 [UniParc].

Last modified November 1, 1998. Version 2.
Checksum: 7094FDD4EE547382
Show »

FASTA

920

100,860

        10         20         30         40         50         60 
MPGRAGVARF CLLALALQLH WPLAACEPGW TTRGSQEGSP PLQHELIIPQ WRTSESPGRG 

        70         80         90        100        110        120 
KHPLRAELRV MAEGRELILD LEKNEHLFAP AYTETCYTAS GNPQTSTLKS EDHCFYHGTV 

       130        140        150        160        170        180 
RDVDESSVTL STCRGIRGLI IVRSNLSYII EPVPNSDSQH RIYRSEHLTL PPGNCGFEHS 

       190        200        210        220        230        240 
GPTSKDWALQ FTHQTKKQPR RMKREDLHSM KYVELYLVAD YAEFQKNRHD QDATKRKLME 

       250        260        270        280        290        300 
IANYVDKFYR SLNIRIALVG LEVWTHGDKC EVSENPYSTL WSFLSWRRKL LAQKSHDNAQ 

       310        320        330        340        350        360 
LITGRSFQGT TIGLAPLMAM CSVYQSGGVS MDHSENAIGV ASTVAHEIGH NFGMSHDSAH 

       370        380        390        400        410        420 
CCSASAADGG CIMAAATGHP FPKVFSWCNR KELDRYLQTG GGMCLSNMPD TRTLYGGRRC 

       430        440        450        460        470        480 
GNGYLEDGEE CDCGEEEECK NPCCNASNCT LKEGAECAHG SCCHQCKLVA PGTQCREQVR 

       490        500        510        520        530        540 
QCDLPEFCTG KSPHCPTNYY QMDGTPCEGG QAYCYNGMCL TYQEQCQQLW GPGARPALDL 

       550        560        570        580        590        600 
CFERVNAAGD TYGNCGKGLN GQYRKCSPRD AKCGKIQCQS TQARPLESNA VSIDTTITLN 

       610        620        630        640        650        660 
GRRIHCRGTH VYRGPEEEEG EGDMLDPGLV MTGTKCGHNH ICFEGQCRNT SFFETEGCGK 

       670        680        690        700        710        720 
KCNGHGVCNN NKNCHCFPGW SPPFCNTPGD GGSVDSGPLP PKSVGPVIAG VFSALFVLAV 

       730        740        750        760        770        780 
LVLLCHCYRQ SHKLGKPSAL PFKLRHQFSC PFRVSQSGGT GHANPTFKLQ TPQGKRKVTN 

       790        800        810        820        830        840 
TPESLRKPSH PPPRPPPDYL RVESPPAPLP AHLNRAAGSS PEAGARIERK ESARRPPPSR 

       850        860        870        880        890        900 
PMPPAPNCLL SQDFSRPRPP QKALPANPVP GQRTGPRSGG TSLLQPPTSG PQPPRPPAVP 

       910        920 
VPKLPEYRSQ RVGAIISSKI

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References

[1]	"Cloning and initial characterization of mouse meltrin beta and analysis of the expression of four metalloprotease-disintegrins in bone cells." Inoue D., Reid M.S., Lum L., Kraetzschmar J., Weskamp G., Myung Y.M., Baron R., Blobel C.P. J. Biol. Chem. 273:4180-4187(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Myoblast.
[2]	"Spatially- and temporally-restricted expression of meltrin alpha (ADAM12) and beta (ADAM19) in mouse embryo." Kurisaki T., Masuda A., Osumi N., Nabeshima Y., Fujisawa-Sehara A. Mech. Dev. 73:211-215(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Tissue: Myoblast.
[3]	"A metalloprotease-disintegrin participating in myoblast fusion." Yagami-Hiromasa T., Sato T., Kurisaki T., Kamijo K., Nabeshima Y., Fujisawa-Sehara A. Nature 377:652-656(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 429-578. Tissue: Embryonic fibroblast.
[4]	"Roles of Meltrin beta /ADAM19 in the processing of neuregulin." Shirakabe K., Wakatsuki S., Kurisaki T., Fujisawa-Sehara A. J. Biol. Chem. 276:9352-9358(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF019887 mRNA. Translation: AAC40037.1. D50410 mRNA. Translation: BAA18923.2.
IPI	IPI00132872.
PIR	PC7067.
RefSeq	NP_033746.1. NM_009616.3.
UniGene	Mm.89940.
3D structure databases
ProteinModelPortal	O35674.
SMR	O35674. Positions 207-651, 661-688.
ModBase	Search...
Protein family/group databases
MEROPS	M12.214.
PTM databases
PhosphoSite	O35674.
Proteomic databases
PRIDE	O35674.
Protocols and materials databases
DNASU	11492.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000011400; ENSMUSP00000011400; ENSMUSG00000011256.
GeneID	11492.
KEGG	mmu:11492.
Organism-specific databases
CTD	8728.
MGI	MGI:105377. Adam19.
Phylogenomic databases
eggNOG	NOG294463.
HOGENOM	HOG000230883.
HOVERGEN	HBG006978.
InParanoid	O35674.
KO	K08608.
OMA	IYRSEHL.
OrthoDB	EOG4K3KVV.
Gene expression databases
ArrayExpress	O35674.
Bgee	O35674.
Genevestigator	O35674.
GermOnline	ENSMUSG00000011256. Mus musculus.
Family and domain databases
Gene3D	3.40.390.10. 1 hit. 4.10.70.10. 1 hit.
InterPro	IPR006586. ADAM_Cys-rich. IPR001762. Blood-coag_inhib_Disintegrin. IPR018358. Disintegrin_CS. IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR024079. MetalloPept_cat_dom. IPR001590. Peptidase_M12B. IPR002870. Peptidase_M12B_N. [Graphical view]
Pfam	PF08516. ADAM_CR. 1 hit. PF00200. Disintegrin. 1 hit. PF01562. Pep_M12B_propep. 1 hit. PF01421. Reprolysin. 1 hit. [Graphical view]
PRINTS	PR00289. DISINTEGRIN.
SMART	SM00608. ACR. 1 hit. SM00050. DISIN. 1 hit. [Graphical view]
SUPFAM	SSF57552. Disintegrin. 1 hit.
PROSITE	PS50215. ADAM_MEPRO. 1 hit. PS00546. CYSTEINE_SWITCH. False negative. PS00427. DISINTEGRIN_1. 1 hit. PS50214. DISINTEGRIN_2. 1 hit. PS00022. EGF_1. False negative. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	278870.
SOURCE	Search...

Entry information

Entry name

ADA19_MOUSE

Accession

Primary (citable) accession number: O35674

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	November 1, 1998
Last modified:	May 1, 2013
This is version 117 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents