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Protein

Disintegrin and metalloproteinase domain-containing protein 19

Gene

Adam19

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell. Also cleaves alpha-2 macroglobulin. May be involved in osteoblast differentiation and/or osteoblast activity in bone (By similarity).By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi133 – 1331Zinc; in inhibited formBy similarity
Metal bindingi346 – 3461Zinc; catalytic
Active sitei347 – 3471
Metal bindingi350 – 3501Zinc; catalytic
Metal bindingi356 – 3561Zinc; catalytic

GO - Molecular functioni

GO - Biological processi

  • heart development Source: MGI
  • membrane protein ectodomain proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM12.214.

Names & Taxonomyi

Protein namesi
Recommended name:
Disintegrin and metalloproteinase domain-containing protein 19 (EC:3.4.24.-)
Short name:
ADAM 19
Alternative name(s):
Meltrin-beta
Gene namesi
Name:Adam19
Synonyms:Mltnb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:105377. Adam19.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 703677ExtracellularSequence AnalysisAdd
BLAST
Transmembranei704 – 72421HelicalSequence AnalysisAdd
BLAST
Topological domaini725 – 920196CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Propeptidei27 – 204178By similarityPRO_0000029104Add
BLAST
Chaini205 – 920716Disintegrin and metalloproteinase domain-containing protein 19PRO_0000029105Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi321 ↔ 404By similarity
Disulfide bondi361 ↔ 388By similarity
Disulfide bondi362 ↔ 371By similarity
Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi448 – 4481N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi475 ↔ 495By similarity
Glycosylationi649 – 6491N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi658 ↔ 668By similarity
Disulfide bondi662 ↔ 674By similarity
Disulfide bondi676 ↔ 685By similarity

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiO35674.

PTM databases

PhosphoSiteiO35674.

Expressioni

Tissue specificityi

Widely expressed, with the highest expression in bone, heart and lung, followed by brain and spleen and relatively low expression in liver, skeletal muscle, kidney and testis. In bone, primarily expressed in cell of the osteoblast lineage and not detected in mature osteoclasts.

Developmental stagei

Expressed in the heart and in the tail bud at 8.0 dpc, and then in the cranial and dorsal root ganglia. Also expressed weakly and transiently in the intestine, lung and in bone marrow.1 Publication

Inductioni

By calcitriol and during osteoblast differentiation.

Gene expression databases

BgeeiO35674.
ExpressionAtlasiO35674. baseline and differential.
GenevestigatoriO35674.

Interactioni

Subunit structurei

Interacts with SH3PXD2A.By similarity

Protein-protein interaction databases

MINTiMINT-4996200.

Structurei

3D structure databases

ProteinModelPortaliO35674.
SMRiO35674. Positions 207-651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 409199Peptidase M12BPROSITE-ProRule annotationAdd
BLAST
Domaini417 – 50387DisintegrinPROSITE-ProRule annotationAdd
BLAST
Domaini654 – 68633EGF-likePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi131 – 1388Cysteine switchBy similarity
Motifi835 – 84612SH3-bindingSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi435 – 4384Poly-Glu
Compositional biasi504 – 653150Cys-richAdd
BLAST
Compositional biasi616 – 6216Poly-Glu

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Contains 1 disintegrin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 1 peptidase M12B domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, SH3-binding, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG294463.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiO35674.
KOiK08608.
OMAiFQKNRRD.
OrthoDBiEOG7F7W89.
PhylomeDBiO35674.
TreeFamiTF314733.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35674-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGRAGVARF CLLALALQLH WPLAACEPGW TTRGSQEGSP PLQHELIIPQ
60 70 80 90 100
WRTSESPGRG KHPLRAELRV MAEGRELILD LEKNEHLFAP AYTETCYTAS
110 120 130 140 150
GNPQTSTLKS EDHCFYHGTV RDVDESSVTL STCRGIRGLI IVRSNLSYII
160 170 180 190 200
EPVPNSDSQH RIYRSEHLTL PPGNCGFEHS GPTSKDWALQ FTHQTKKQPR
210 220 230 240 250
RMKREDLHSM KYVELYLVAD YAEFQKNRHD QDATKRKLME IANYVDKFYR
260 270 280 290 300
SLNIRIALVG LEVWTHGDKC EVSENPYSTL WSFLSWRRKL LAQKSHDNAQ
310 320 330 340 350
LITGRSFQGT TIGLAPLMAM CSVYQSGGVS MDHSENAIGV ASTVAHEIGH
360 370 380 390 400
NFGMSHDSAH CCSASAADGG CIMAAATGHP FPKVFSWCNR KELDRYLQTG
410 420 430 440 450
GGMCLSNMPD TRTLYGGRRC GNGYLEDGEE CDCGEEEECK NPCCNASNCT
460 470 480 490 500
LKEGAECAHG SCCHQCKLVA PGTQCREQVR QCDLPEFCTG KSPHCPTNYY
510 520 530 540 550
QMDGTPCEGG QAYCYNGMCL TYQEQCQQLW GPGARPALDL CFERVNAAGD
560 570 580 590 600
TYGNCGKGLN GQYRKCSPRD AKCGKIQCQS TQARPLESNA VSIDTTITLN
610 620 630 640 650
GRRIHCRGTH VYRGPEEEEG EGDMLDPGLV MTGTKCGHNH ICFEGQCRNT
660 670 680 690 700
SFFETEGCGK KCNGHGVCNN NKNCHCFPGW SPPFCNTPGD GGSVDSGPLP
710 720 730 740 750
PKSVGPVIAG VFSALFVLAV LVLLCHCYRQ SHKLGKPSAL PFKLRHQFSC
760 770 780 790 800
PFRVSQSGGT GHANPTFKLQ TPQGKRKVTN TPESLRKPSH PPPRPPPDYL
810 820 830 840 850
RVESPPAPLP AHLNRAAGSS PEAGARIERK ESARRPPPSR PMPPAPNCLL
860 870 880 890 900
SQDFSRPRPP QKALPANPVP GQRTGPRSGG TSLLQPPTSG PQPPRPPAVP
910 920
VPKLPEYRSQ RVGAIISSKI
Length:920
Mass (Da):100,860
Last modified:November 1, 1998 - v2
Checksum:i7094FDD4EE547382
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019887 mRNA. Translation: AAC40037.1.
D50410 mRNA. Translation: BAA18923.2.
CCDSiCCDS24571.1.
PIRiPC7067.
RefSeqiNP_033746.1. NM_009616.4.
UniGeneiMm.89940.

Genome annotation databases

EnsembliENSMUST00000011400; ENSMUSP00000011400; ENSMUSG00000011256.
GeneIDi11492.
KEGGimmu:11492.
UCSCiuc007iny.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019887 mRNA. Translation: AAC40037.1.
D50410 mRNA. Translation: BAA18923.2.
CCDSiCCDS24571.1.
PIRiPC7067.
RefSeqiNP_033746.1. NM_009616.4.
UniGeneiMm.89940.

3D structure databases

ProteinModelPortaliO35674.
SMRiO35674. Positions 207-651.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996200.

Protein family/group databases

MEROPSiM12.214.

PTM databases

PhosphoSiteiO35674.

Proteomic databases

PRIDEiO35674.

Protocols and materials databases

DNASUi11492.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000011400; ENSMUSP00000011400; ENSMUSG00000011256.
GeneIDi11492.
KEGGimmu:11492.
UCSCiuc007iny.1. mouse.

Organism-specific databases

CTDi8728.
MGIiMGI:105377. Adam19.

Phylogenomic databases

eggNOGiNOG294463.
HOGENOMiHOG000230883.
HOVERGENiHBG006978.
InParanoidiO35674.
KOiK08608.
OMAiFQKNRRD.
OrthoDBiEOG7F7W89.
PhylomeDBiO35674.
TreeFamiTF314733.

Miscellaneous databases

NextBioi278870.
PROiO35674.
SOURCEiSearch...

Gene expression databases

BgeeiO35674.
ExpressionAtlasiO35674. baseline and differential.
GenevestigatoriO35674.

Family and domain databases

Gene3Di3.40.390.10. 1 hit.
4.10.70.10. 1 hit.
InterProiIPR006586. ADAM_Cys-rich.
IPR001762. Blood-coag_inhib_Disintegrin.
IPR018358. Disintegrin_CS.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR024079. MetalloPept_cat_dom.
IPR001590. Peptidase_M12B.
IPR002870. Peptidase_M12B_N.
[Graphical view]
PfamiPF08516. ADAM_CR. 1 hit.
PF00200. Disintegrin. 1 hit.
PF01562. Pep_M12B_propep. 1 hit.
PF01421. Reprolysin. 1 hit.
[Graphical view]
PRINTSiPR00289. DISINTEGRIN.
SMARTiSM00608. ACR. 1 hit.
SM00050. DISIN. 1 hit.
[Graphical view]
SUPFAMiSSF57552. SSF57552. 1 hit.
PROSITEiPS50215. ADAM_MEPRO. 1 hit.
PS00427. DISINTEGRIN_1. 1 hit.
PS50214. DISINTEGRIN_2. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and initial characterization of mouse meltrin beta and analysis of the expression of four metalloprotease-disintegrins in bone cells."
    Inoue D., Reid M.S., Lum L., Kraetzschmar J., Weskamp G., Myung Y.M., Baron R., Blobel C.P.
    J. Biol. Chem. 273:4180-4187(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Myoblast.
  2. "Spatially- and temporally-restricted expression of meltrin alpha (ADAM12) and beta (ADAM19) in mouse embryo."
    Kurisaki T., Masuda A., Osumi N., Nabeshima Y., Fujisawa-Sehara A.
    Mech. Dev. 73:211-215(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE.
    Tissue: Myoblast.
  3. "A metalloprotease-disintegrin participating in myoblast fusion."
    Yagami-Hiromasa T., Sato T., Kurisaki T., Kamijo K., Nabeshima Y., Fujisawa-Sehara A.
    Nature 377:652-656(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 429-578.
    Tissue: Embryonic fibroblast.
  4. "Roles of Meltrin beta /ADAM19 in the processing of neuregulin."
    Shirakabe K., Wakatsuki S., Kurisaki T., Fujisawa-Sehara A.
    J. Biol. Chem. 276:9352-9358(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiADA19_MOUSE
AccessioniPrimary (citable) accession number: O35674
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 1, 1998
Last modified: January 7, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.