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Protein

Integrin beta-1-binding protein 1

Gene

Itgb1bp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key regulator of the integrin-mediated cell-matrix interaction signaling by binding to the ITGB1 cytoplasmic tail and preventing the activation of integrin alpha-5/beta-1 (heterodimer of ITGA5 and ITGB1) by talin or FERMT1. Plays a role in cell proliferation, differentiation, spreading, adhesion and migration in the context of mineralization and bone development and angiogenesis. Stimulates cellular proliferation in a fibronectin-dependent manner. Involved in the regulation of beta-1 integrin-containing focal adhesion (FA) site dynamics by controlling its assembly rate during cell adhesion; inhibits beta-1 integrin clustering within FA by directly competing with talin TLN1, and hence stimulates osteoblast spreading and migration in a fibronectin-and/or collagen-dependent manner. Acts as a guanine nucleotide dissociation inhibitor (GDI) by regulating Rho family GTPases during integrin-mediated cell matrix adhesion; reduces the level of active GTP-bound form of both CDC42 and RAC1 GTPases upon cell adhesion to fibronectin. Stimulates the release of active CDC42 from the membranes to maintain it in an inactive cytoplasmic pool. Participates in the translocation of the Rho-associated protein kinase ROCK1 to membrane ruffles at cell leading edges of the cell membrane, leading to an increase of myoblast cell migration on laminin. Plays a role in bone mineralization at a late stage of osteoblast differentiation; modulates the dynamic formation of focal adhesions into fibrillar adhesions, which are adhesive structures responsible for fibronectin deposition and fibrillogenesis. Plays a role in blood vessel development; acts as a negative regulator of angiogenesis by attenuating endothelial cell proliferation and migration, lumen formation and sprouting angiogenesis by promoting AKT phosphorylation and inhibiting ERK1/2 phosphorylation through activation of the Notch signaling pathway. Promotes transcriptional activity of the MYC promoter.5 Publications

GO - Molecular functioni

  • GDP-dissociation inhibitor activity Source: UniProtKB
  • integrin binding Source: MGI
  • kinase binding Source: UniProtKB
  • protein complex binding Source: UniProtKB
  • protein C-terminus binding Source: MGI
  • protein kinase binding Source: UniProtKB
  • protein transporter activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Mitogen

Keywords - Biological processi

Angiogenesis, Biomineralization, Cell adhesion, Differentiation, Notch signaling pathway, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-1-binding protein 1
Alternative name(s):
Bodenin
Gene namesi
Name:Itgb1bp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1306802. Itgb1bp1.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytoskeleton Source: UniProtKB
  • cytosol Source: HGNC
  • extracellular exosome Source: MGI
  • lamellipodium Source: UniProtKB
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • ruffle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show severe defects in osteoblast mesenchymal cells to compaction, proliferation, differentiation, and mineralization and to a delay in bone nodule formation. Suffer also from an excessive microvessel growth.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 200200Integrin beta-1-binding protein 1PRO_0000084265Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei38 – 381Phosphothreonine; by CaMK2By similarity
Modified residuei41 – 411PhosphoserineCombined sources

Post-translational modificationi

Phosphorylation at Thr-38 seems to enhance integrin alpha5beta1-mediated cell adhesion. The degree of phosphorylation is regulated by integrin-dependent cell-matrix interaction (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO35671.
MaxQBiO35671.
PaxDbiO35671.
PRIDEiO35671.

PTM databases

PhosphoSiteiO35671.

Expressioni

Tissue specificityi

Expressed in the brain.

Developmental stagei

First expressed in the heart primordium at E8.5.

Gene expression databases

BgeeiO35671.
CleanExiMM_ITGB1BP1.
ExpressionAtlasiO35671. baseline and differential.
GenevisibleiO35671. MM.

Interactioni

Subunit structurei

Found in a complex, at least composed of ITGB1BP1, KRIT1 and RAP1A. Interacts (via C-terminal region) with ITGB1 (via C-terminal cytoplasmic tail); the interaction prevents talin TLN1 binding to ITGB1 and KRIT1 and ITGB1 compete for the same binding site. Interacts with KRIT1 (via N-terminal NPXY motif); the interaction induces the opening conformation of KRIT1 and KRIT1 and ITGB1 compete for the same binding site. Isoform 2 does not interact with ITGB1. Interacts with CDC42 (GTP- or GDP-bound form); the interaction is increased with the CDC42-membrane bound forms and prevents both CDC42 activation and cell spreading. Interacts (via C-terminal domain region) with NME2. Interacts with FERMT2 and RAC1 (By similarity). Interacts (via N-terminus and PTB domain) with ROCK1.By similarity2 Publications

GO - Molecular functioni

  • integrin binding Source: MGI
  • kinase binding Source: UniProtKB
  • protein complex binding Source: UniProtKB
  • protein C-terminus binding Source: MGI
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075609.

Structurei

3D structure databases

ProteinModelPortaliO35671.
SMRiO35671. Positions 58-196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 200143PIDAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 1394Interaction with KRIT1By similarity
Regioni139 – 1413Interaction with ITGB1By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi6 – 72Nuclear localization signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi10 – 5546Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Contains 1 PID domain.Curated

Phylogenomic databases

eggNOGiENOG410IFN6. Eukaryota.
ENOG4111GWT. LUCA.
GeneTreeiENSGT00390000003990.
HOGENOMiHOG000015089.
HOVERGENiHBG052155.
InParanoidiO35671.
OMAiRMLCYDD.
OrthoDBiEOG7WT447.
PhylomeDBiO35671.
TreeFamiTF105393.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR019517. Integrin-bd_ICAP-1.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamiPF10480. ICAP-1_inte_bdg. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.

Sequencei

Sequence statusi: Complete.

O35671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRKGKKRHS SSSSQSSEIS TKSKSVDSSL GGLSRSSTVA SLDTDSTKSS
60 70 80 90 100
GQSNSNLDTC AEFRIKYVGA IEKLAVSEGK SLEGPLDLIN YIDVAQQDGK
110 120 130 140 150
LPFVPLEEEF ILGVSKYGIK VSTTDQHGVL HRHALYLIIR MVCYDDGLGA
160 170 180 190 200
GKSLLALKTT DASNEEYSLW VYQCNSLEQA QAICKVLSTA FDSVLTSDKS
Length:200
Mass (Da):21,644
Last modified:January 1, 1998 - v1
Checksum:iF7F0EB09490AEA37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001373 mRNA. Translation: CAA04706.1.
AK002786 mRNA. Translation: BAB22357.1.
AK004172 mRNA. Translation: BAB23206.1.
AK007635 mRNA. Translation: BAB25151.1.
AK075577 mRNA. Translation: BAC35832.1.
AK089937 mRNA. Translation: BAC41007.1.
BC028772 mRNA. Translation: AAH28772.1.
CCDSiCCDS25833.1.
RefSeqiNP_032429.1. NM_008403.4.
XP_006515054.1. XM_006514991.2.
UniGeneiMm.273333.

Genome annotation databases

EnsembliENSMUST00000076260; ENSMUSP00000075609; ENSMUSG00000062352.
ENSMUST00000173729; ENSMUSP00000134627; ENSMUSG00000062352.
GeneIDi16413.
KEGGimmu:16413.
UCSCiuc007ndl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001373 mRNA. Translation: CAA04706.1.
AK002786 mRNA. Translation: BAB22357.1.
AK004172 mRNA. Translation: BAB23206.1.
AK007635 mRNA. Translation: BAB25151.1.
AK075577 mRNA. Translation: BAC35832.1.
AK089937 mRNA. Translation: BAC41007.1.
BC028772 mRNA. Translation: AAH28772.1.
CCDSiCCDS25833.1.
RefSeqiNP_032429.1. NM_008403.4.
XP_006515054.1. XM_006514991.2.
UniGeneiMm.273333.

3D structure databases

ProteinModelPortaliO35671.
SMRiO35671. Positions 58-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000075609.

PTM databases

PhosphoSiteiO35671.

Proteomic databases

EPDiO35671.
MaxQBiO35671.
PaxDbiO35671.
PRIDEiO35671.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000076260; ENSMUSP00000075609; ENSMUSG00000062352.
ENSMUST00000173729; ENSMUSP00000134627; ENSMUSG00000062352.
GeneIDi16413.
KEGGimmu:16413.
UCSCiuc007ndl.1. mouse.

Organism-specific databases

CTDi9270.
MGIiMGI:1306802. Itgb1bp1.

Phylogenomic databases

eggNOGiENOG410IFN6. Eukaryota.
ENOG4111GWT. LUCA.
GeneTreeiENSGT00390000003990.
HOGENOMiHOG000015089.
HOVERGENiHBG052155.
InParanoidiO35671.
OMAiRMLCYDD.
OrthoDBiEOG7WT447.
PhylomeDBiO35671.
TreeFamiTF105393.

Miscellaneous databases

NextBioi289601.
PROiO35671.
SOURCEiSearch...

Gene expression databases

BgeeiO35671.
CleanExiMM_ITGB1BP1.
ExpressionAtlasiO35671. baseline and differential.
GenevisibleiO35671. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR019517. Integrin-bd_ICAP-1.
IPR011993. PH_dom-like.
IPR006020. PTB/PI_dom.
[Graphical view]
PfamiPF10480. ICAP-1_inte_bdg. 1 hit.
[Graphical view]
SMARTiSM00462. PTB. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bodenin: a novel murine gene expressed in restricted areas of the brain."
    Faisst A.M., Gruss P.
    Dev. Dyn. 212:293-303(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Kidney and Pancreas.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. "Integrin cytoplasmic domain-associated protein-1 (ICAP-1) interacts with the ROCK-I kinase at the plasma membrane."
    Stroeken P.J., Alvarez B., Van Rheenen J., Wijnands Y.M., Geerts D., Jalink K., Roos E.
    J. Cell. Physiol. 208:620-628(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ROCK1, SUBCELLULAR LOCATION.
  5. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  6. "Cell adaptive response to extracellular matrix density is controlled by ICAP-1-dependent beta1-integrin affinity."
    Millon-Fremillon A., Bouvard D., Grichine A., Manet-Dupe S., Block M.R., Albiges-Rizo C.
    J. Cell Biol. 180:427-441(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Integrin Cytoplasmic domain-Associated Protein-1 (ICAP-1) promotes migration of myoblasts and affects focal adhesions."
    Alvarez B., Stroeken P.J., Edel M.J., Roos E.
    J. Cell. Physiol. 214:474-482(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ROCK1.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung, Spleen and Testis.
  9. "Osteoblast mineralization requires beta1 integrin/ICAP-1-dependent fibronectin deposition."
    Brunner M., Millon-Fremillon A., Chevalier G., Nakchbandi I.A., Mosher D., Block M.R., Albiges-Rizo C., Bouvard D.
    J. Cell Biol. 194:307-322(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiITBP1_MOUSE
AccessioniPrimary (citable) accession number: O35671
Secondary accession number(s): Q542A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 1, 1998
Last modified: March 16, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.