O35660 (GSTM6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase Mu 6
EC=2.5.1.18

Alternative name(s):
GST class-mu 6
Glutathione-S-transferase class M5

Gene names

Name:	Gstm6
Synonyms:	Gstm5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Expressed in liver, stomach and small intestine. Not expressed in spleen, kidney, colon, heart, muscle, brain or lung. Ref.1
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Glutathione S-transferase Mu 6		PRO_0000185830
Regions
	Domain	1 – 88	88	GST N-terminal
	Domain	90 – 208	119	GST C-terminal
	Region	7 – 8	2	Glutathione binding By similarity
	Region	46 – 50	5	Glutathione binding By similarity
	Region	59 – 60	2	Glutathione binding By similarity
	Region	72 – 73	2	Glutathione binding By similarity
Sites
	Binding site	116	1	Substrate By similarity
Experimental info
	Sequence conflict	31	1	R → K in AAH31818. Ref.2
	Sequence conflict	54	1	G → D in AAH31818. Ref.2
	Sequence conflict	102 – 103	2	NR → KQ in AAH31818. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O35660 [UniParc]. Last modified August 16, 2004. Version 3. Checksum: DBACBEBCFB3AF314	FASTA	218	25,605
	10 20 30 40 50 60 MPVTLGYWDI RGLGHAIRLL LEYTETGYEE RRYAMGDAPD YDRSQWLNDK FKLGLDFPNL 70 80 90 100 110 120 PYLIDGSHKV TQSNAILRYL GRKHNLCGET EEERIRVDIL ENRVMDTRIQ MGMLCYSADF 130 140 150 160 170 180 EKRKPEFLKG LPDQLKLYSE FLGKQPWFAG DKITFADFLV YDVLDQHRMF EPTCLDAFPN 190 200 210 LKDFMARFEG LRKISAYMKT SRFLPSPVYL KQATWGNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel murine glutathione S-transferase class mu gene." De Bruin W.C.C., te Morsche R.H.M., Wagenmans M.J.M., Alferink J.C., Townsend A.J., Wieringa B., Peters W.H.M. Biochem. J. 330:623-626(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY. Strain: 129/Sv, C57BL/6 and FVB. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ000413 mRNA. Translation: CAA04061.1. AJ000412 Genomic DNA. Translation: CAA04060.1. BC031818 mRNA. Translation: AAH31818.1.
IPI	IPI00648675.
RefSeq	NP_032210.3. NM_008184.3.
UniGene	Mm.458154.
3D structure databases
ProteinModelPortal	O35660.
SMR	O35660. Positions 2-215.
ModBase	Search...
PTM databases
PhosphoSite	O35660.
Proteomic databases
PaxDb	O35660.
PRIDE	O35660.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000106685; ENSMUSP00000102296; ENSMUSG00000068762.
GeneID	14867.
KEGG	mmu:14867.
Organism-specific databases
CTD	14867.
MGI	MGI:1309467. Gstm6.
Phylogenomic databases
eggNOG	NOG05174.
GeneTree	ENSGT00550000074559.
HOGENOM	HOG000115735.
HOVERGEN	HBG106842.
KO	K00799.
OrthoDB	EOG4XWG0R.
Gene expression databases
ArrayExpress	O35660.
Bgee	O35660.
CleanEx	MM_GSTM5. MM_GSTM6.
Genevestigator	O35660.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR003081. GST_mu. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01267. GSTRNSFRASEM.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GSTM5. mouse.
NextBio	287125.
SOURCE	Search...

Entry information

Entry name

GSTM6_MOUSE

Accession

Primary (citable) accession number: O35660
Secondary accession number(s): O35661, Q8K0C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	August 16, 2004
Last modified:	April 3, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program