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Protein

Sialidase-1

Gene

Neu1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage.2 Publications

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Binding sitei91SubstrateBy similarity1
Active sitei97Proton acceptorBy similarity1
Active sitei258Sequence analysis1
Binding sitei258SubstrateBy similarity1
Binding sitei274SubstrateBy similarity1
Binding sitei335SubstrateBy similarity1
Active sitei364NucleophileBy similarity1
Active sitei388Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.2.1.18. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-4085001. Sialic acid metabolism.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-1 (EC:3.2.1.18)
Alternative name(s):
G9 sialidase
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1
Gene namesi
Name:Neu1
Synonyms:Neu
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:97305. Neu1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 41Sequence analysisAdd BLAST41
ChainiPRO_000001202742 – 409Sialidase-1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi180N-linked (GlcNAc...)Sequence analysis1
Glycosylationi337N-linked (GlcNAc...)Sequence analysis1
Glycosylationi346N-linked (GlcNAc...)Sequence analysis1
Glycosylationi372N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.Curated
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiO35657.
PaxDbiO35657.
PeptideAtlasiO35657.
PRIDEiO35657.

PTM databases

iPTMnetiO35657.
PhosphoSitePlusiO35657.
SwissPalmiO35657.

Expressioni

Tissue specificityi

Highly expressed in kidney, epididymis, followed by brain, spinal cord and weakly expressed in adrenal, heart, liver, lung and spleen.1 Publication

Gene expression databases

BgeeiENSMUSG00000007038.
CleanExiMM_NEU1.
ExpressionAtlasiO35657. baseline and differential.
GenevisibleiO35657. MM.

Interactioni

Subunit structurei

Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.

Protein-protein interaction databases

BioGridi201731. 1 interactor.
STRINGi10090.ENSMUSP00000007253.

Structurei

3D structure databases

ProteinModelPortaliO35657.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati106 – 117BNR 1Add BLAST12
Repeati166 – 177BNR 2Add BLAST12
Repeati225 – 236BNR 3Add BLAST12
Repeati341 – 352BNR 4Add BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi71 – 74FRIP motif4
Motifi406 – 409Internalization signal4

Domaini

A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IE33. Eukaryota.
COG4409. LUCA.
GeneTreeiENSGT00720000108878.
HOGENOMiHOG000007651.
HOVERGENiHBG057314.
InParanoidiO35657.
KOiK01186.
OMAiRNQYTES.
OrthoDBiEOG091G0HAW.
PhylomeDBiO35657.
TreeFamiTF331063.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O35657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGADPTRPR GPLSYWAGRR GQGLAAIFLL LVSAAESEAR AEDDFSLVQP
60 70 80 90 100
LVTMEQLLWV SGKQIGSVDT FRIPLITATP RGTLLAFAEA RKKSASDEGA
110 120 130 140 150
KFIAMRRSTD QGSTWSSTAF IVDDGEASDG LNLGAVVNDV DTGIVFLIYT
160 170 180 190 200
LCAHKVNCQV ASTMLVWSKD DGISWSPPRN LSVDIGTEMF APGPGSGIQK
210 220 230 240 250
QREPGKGRLI VCGHGTLERD GVFCLLSDDH GASWHYGTGV SGIPFGQPKH
260 270 280 290 300
DHDFNPDECQ PYELPDGSVI INARNQNNYH CRCRIVLRSY DACDTLRPRD
310 320 330 340 350
VTFDPELVDP VVAAGALATS SGIVFFSNPA HPEFRVNLTL RWSFSNGTSW
360 370 380 390 400
QKERVQVWPG PSGYSSLTAL ENSTDGKKQP PQLFVLYEKG LNRYTESISM

VKISVYGTL
Length:409
Mass (Da):44,591
Last modified:January 1, 1998 - v1
Checksum:i416BFD5BE27B8893
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107R → K in AAC53536 (PubMed:9384611).Curated1
Sequence conflicti113S → C in AAC53536 (PubMed:9384611).Curated1
Sequence conflicti117 – 121STAFI → PTGFM in AAC53536 (PubMed:9384611).Curated5
Sequence conflicti172 – 173GI → AF in AAC53536 (PubMed:9384611).Curated2
Sequence conflicti344F → L in AAC53536 (PubMed:9384611).Curated1
Sequence conflicti351Q → L in AAH04666 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti209L → I Reduced activity. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11412 mRNA. Translation: CAA72215.1.
U93702 mRNA. Translation: AAC53536.1.
AF109906 Genomic DNA. Translation: AAC84167.1.
BC004666 mRNA. Translation: AAH04666.1.
CCDSiCCDS28668.1.
RefSeqiNP_035023.3. NM_010893.3.
UniGeneiMm.8856.

Genome annotation databases

EnsembliENSMUST00000007253; ENSMUSP00000007253; ENSMUSG00000007038.
GeneIDi18010.
KEGGimmu:18010.
UCSCiuc008cei.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11412 mRNA. Translation: CAA72215.1.
U93702 mRNA. Translation: AAC53536.1.
AF109906 Genomic DNA. Translation: AAC84167.1.
BC004666 mRNA. Translation: AAH04666.1.
CCDSiCCDS28668.1.
RefSeqiNP_035023.3. NM_010893.3.
UniGeneiMm.8856.

3D structure databases

ProteinModelPortaliO35657.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201731. 1 interactor.
STRINGi10090.ENSMUSP00000007253.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

PTM databases

iPTMnetiO35657.
PhosphoSitePlusiO35657.
SwissPalmiO35657.

Proteomic databases

EPDiO35657.
PaxDbiO35657.
PeptideAtlasiO35657.
PRIDEiO35657.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007253; ENSMUSP00000007253; ENSMUSG00000007038.
GeneIDi18010.
KEGGimmu:18010.
UCSCiuc008cei.2. mouse.

Organism-specific databases

CTDi4758.
MGIiMGI:97305. Neu1.

Phylogenomic databases

eggNOGiENOG410IE33. Eukaryota.
COG4409. LUCA.
GeneTreeiENSGT00720000108878.
HOGENOMiHOG000007651.
HOVERGENiHBG057314.
InParanoidiO35657.
KOiK01186.
OMAiRNQYTES.
OrthoDBiEOG091G0HAW.
PhylomeDBiO35657.
TreeFamiTF331063.

Enzyme and pathway databases

BRENDAi3.2.1.18. 3474.
ReactomeiR-MMU-1660662. Glycosphingolipid metabolism.
R-MMU-4085001. Sialic acid metabolism.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiNeu1. mouse.
PROiO35657.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000007038.
CleanExiMM_NEU1.
ExpressionAtlasiO35657. baseline and differential.
GenevisibleiO35657. MM.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNEUR1_MOUSE
AccessioniPrimary (citable) accession number: O35657
Secondary accession number(s): O55220, Q99KG9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.