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O35655 (PPE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase with EF-hands 1
Short name=PPEF-1
EC=3.1.3.16
Alternative name(s):
DRES10
Protein phosphatase with EF calcium-binding domain
Short name=PPEF
Gene names
Name:Ppef1
Synonyms:Dres10, Ppef
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length650 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May have a role in the recovery or adaptation response of photoreceptors. May have a role in diverse sensory neurons and in development.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 manganese ion per subunit By similarity.

Magnesium By similarity.

Enzyme regulation

Activated by calcium By similarity.

Tissue specificity

In the embryo it is almost exclusively expressed in the peripheral nervous system, within sensory neurons of cranial and dorsal root ganglia. Otherwise found in fetal inner ear and a small group of neurons in the midbrain/pons jonction.

Developmental stage

Up-regulated at E12.3 in dorsal root ganglia (DRG) and in some sensory cranial ganglia. A slightly decreased expression could still be detected in sensory ganglia at E16.5. It is not known if expression in sensory neurons persists in adult life.

Sequence similarities

Belongs to the PPP phosphatase family.

Contains 3 EF-hand domains.

Contains 1 IQ domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 650650Serine/threonine-protein phosphatase with EF-hands 1
PRO_0000058900

Regions

Domain16 – 4530IQ
Domain484 – 51936EF-hand 1
Domain567 – 60236EF-hand 2
Domain607 – 64236EF-hand 3
Calcium binding497 – 508121 Potential
Calcium binding580 – 591122 Potential
Calcium binding620 – 631123 Potential
Region124 – 456333Catalytic By similarity

Sites

Active site2371Proton donor By similarity
Metal binding1751Iron By similarity
Metal binding1771Iron By similarity
Metal binding2041Iron By similarity
Metal binding2041Manganese By similarity
Metal binding2361Manganese By similarity
Metal binding2881Manganese By similarity
Metal binding4041Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
O35655 [UniParc].

Last modified July 10, 2007. Version 2.
Checksum: F5B349BE006B60F5

FASTA65075,058
        10         20         30         40         50         60 
MGCGTSSKKG NKSKKVIKAA LVIQNWYRRY RARLRVRQHY ALAIFQSIEY SDEQGQMQLS 

        70         80         90        100        110        120 
SFFSFMLENY TKTNNEDSAL VTRIFDNTRR ESQIKDRDDF LGLIEVPDSY DGPRLQFPLT 

       130        140        150        160        170        180 
FTDIHILLQA FKQQQILHAH YVLEVLFEAR KVLKQMPNFS HVKTFPAKEI TICGDLHGKL 

       190        200        210        220        230        240 
DDLMLIFYKN GLPSENNPYV FNGDFVDRGN NSMEILMILL VCFLVYPSDL HLNRGNHEDF 

       250        260        270        280        290        300 
MMNLRYGFTK EILQKYKLHG RKILQVLEEV YTWLPIGTII DNEILVIHGG ISESTDLNTL 

       310        320        330        340        350        360 
HQLQRNKMKS VLMPPVLGNQ ETGEKRNKSA SNYVEPRKVE PDKTPSEDLT KQEWEQIVDI 

       370        380        390        400        410        420 
LWSDPRGKKG CYPNTSRGGG CYFGPDVTSK VLSKNQLKML IRSHECKPDG YEVSHDGKVI 

       430        440        450        460        470        480 
TVFSASNYYE EGSNRGAYIR LSYGTMPQFF QYQVTSTSCL NPLHQRMNAM ESSAFKILKE 

       490        500        510        520        530        540 
KMISRKTDLI NAFELRDHSR SGRISLAEWA FSMENILGLN LPWRSLSSHL VTIDSSGSVD 

       550        560        570        580        590        600 
YMSSFDDIRI EKPTKDMKSN LTETMYRYRS DLKIIFNIID SDQSGLISMD EFRTMWKLFN 

       610        620        630        640        650 
AHYKAHIDDS QIDELASIVD FNKDGNIDFN EFLKAFYVVH KYDKPGTSLA

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"A novel human serine-threonine phosphatase related to the Drosophila retinal degeneration C (rdgC) gene is selectively expressed in sensory neurons of neural crest origin."
Montini E., Rugarli E.I., van de Vosse E., Andolfi G., Mariani M., Puca A.A., Consales G.G., den Dunnen J.T., Ballabio A., Franco B.
Hum. Mol. Genet. 6:1137-1145(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-253.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL732450, AL974311 Genomic DNA. Translation: CAM17008.1.
AL974311, AL732450 Genomic DNA. Translation: CAM19040.1.
Y08234 Genomic DNA. Translation: CAA69403.1.
IPIIPI00671133.
RefSeqNP_035277.1. NM_011147.1.
UniGeneMm.296779.

3D structure databases

ProteinModelPortalO35655.
SMRO35655. Positions 110-452, 471-634.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000071191.

PTM databases

PhosphoSiteO35655.

Proteomic databases

PRIDEO35655.

Protocols and materials databases

DNASU237178.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000071204; ENSMUSP00000071191; ENSMUSG00000062168.
GeneID237178.
KEGGmmu:237178.
UCSCuc009uto.1. mouse.

Organism-specific databases

CTD5475.
MGIMGI:1097157. Ppef1.

Phylogenomic databases

eggNOGCOG0639.
GeneTreeENSGT00530000063173.
HOGENOMHOG000006820.
HOVERGENHBG008238.
InParanoidO35655.
KOK01090.
OrthoDBEOG4255SB.

Gene expression databases

ArrayExpressO35655.
BgeeO35655.
GenevestigatorO35655.

Family and domain databases

Gene3D1.10.238.10. 2 hits.
InterProIPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR004843. Metallo_PEstase_dom.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF13499. EF_hand_5. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PIRSFPIRSF000912. PPEF. 1 hit.
PRINTSPR00114. STPHPHTASE.
SMARTSM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS50096. IQ. False negative.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio383242.
SOURCESearch...

Entry information

Entry namePPE1_MOUSE
AccessionPrimary (citable) accession number: O35655
Secondary accession number(s): A2AIC6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: July 10, 2007
Last modified: May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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