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O35655

- PPE1_MOUSE

UniProt

O35655 - PPE1_MOUSE

Protein

Serine/threonine-protein phosphatase with EF-hands 1

Gene

Ppef1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    May have a role in the recovery or adaptation response of photoreceptors. May have a role in diverse sensory neurons and in development.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity
    Magnesium.By similarity

    Enzyme regulationi

    Activated by calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi175 – 1751Manganese 1By similarity
    Metal bindingi177 – 1771Manganese 1By similarity
    Metal bindingi204 – 2041Manganese 1By similarity
    Metal bindingi204 – 2041Manganese 2By similarity
    Metal bindingi236 – 2361Manganese 2By similarity
    Active sitei237 – 2371Proton donorBy similarity
    Metal bindingi288 – 2881Manganese 2By similarity
    Metal bindingi404 – 4041Manganese 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi497 – 508121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi580 – 591122PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi620 – 631123PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. manganese ion binding Source: InterPro
    4. protein serine/threonine phosphatase activity Source: MGI

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. detection of stimulus involved in sensory perception Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase with EF-hands 1 (EC:3.1.3.16)
    Short name:
    PPEF-1
    Alternative name(s):
    DRES10
    Protein phosphatase with EF calcium-binding domain
    Short name:
    PPEF
    Gene namesi
    Name:Ppef1
    Synonyms:Dres10, Ppef
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1097157. Ppef1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 650650Serine/threonine-protein phosphatase with EF-hands 1PRO_0000058900Add
    BLAST

    Proteomic databases

    MaxQBiO35655.
    PRIDEiO35655.

    PTM databases

    PhosphoSiteiO35655.

    Expressioni

    Tissue specificityi

    In the embryo it is almost exclusively expressed in the peripheral nervous system, within sensory neurons of cranial and dorsal root ganglia. Otherwise found in fetal inner ear and a small group of neurons in the midbrain/pons junction.

    Developmental stagei

    Up-regulated at E12.3 in dorsal root ganglia (DRG) and in some sensory cranial ganglia. A slightly decreased expression could still be detected in sensory ganglia at E16.5. It is not known if expression in sensory neurons persists in adult life.

    Gene expression databases

    BgeeiO35655.
    GenevestigatoriO35655.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000071191.

    Structurei

    3D structure databases

    ProteinModelPortaliO35655.
    SMRiO35655. Positions 72-638.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 4530IQAdd
    BLAST
    Domaini484 – 51936EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini567 – 60236EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini607 – 64236EF-hand 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 456333CatalyticBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family.Curated
    Contains 3 EF-hand domains.PROSITE-ProRule annotation
    Contains 1 IQ domain.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063173.
    HOGENOMiHOG000006820.
    HOVERGENiHBG008238.
    InParanoidiO35655.
    KOiK13807.
    OMAiMMNLRYG.
    OrthoDBiEOG7KH9J7.
    TreeFamiTF313342.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    3.60.21.10. 2 hits.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR029052. Metallo-depent_PP-like.
    IPR013235. PPP_dom.
    IPR012008. Ser/Thr-Pase_EF-hand_contain.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 1 hit.
    PF00149. Metallophos. 1 hit.
    PF08321. PPP5. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000912. PPEF. 1 hit.
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00054. EFh. 3 hits.
    SM00015. IQ. 1 hit.
    SM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 2 hits.
    PROSITEiPS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O35655-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCGTSSKKG NKSKKVIKAA LVIQNWYRRY RARLRVRQHY ALAIFQSIEY    50
    SDEQGQMQLS SFFSFMLENY TKTNNEDSAL VTRIFDNTRR ESQIKDRDDF 100
    LGLIEVPDSY DGPRLQFPLT FTDIHILLQA FKQQQILHAH YVLEVLFEAR 150
    KVLKQMPNFS HVKTFPAKEI TICGDLHGKL DDLMLIFYKN GLPSENNPYV 200
    FNGDFVDRGN NSMEILMILL VCFLVYPSDL HLNRGNHEDF MMNLRYGFTK 250
    EILQKYKLHG RKILQVLEEV YTWLPIGTII DNEILVIHGG ISESTDLNTL 300
    HQLQRNKMKS VLMPPVLGNQ ETGEKRNKSA SNYVEPRKVE PDKTPSEDLT 350
    KQEWEQIVDI LWSDPRGKKG CYPNTSRGGG CYFGPDVTSK VLSKNQLKML 400
    IRSHECKPDG YEVSHDGKVI TVFSASNYYE EGSNRGAYIR LSYGTMPQFF 450
    QYQVTSTSCL NPLHQRMNAM ESSAFKILKE KMISRKTDLI NAFELRDHSR 500
    SGRISLAEWA FSMENILGLN LPWRSLSSHL VTIDSSGSVD YMSSFDDIRI 550
    EKPTKDMKSN LTETMYRYRS DLKIIFNIID SDQSGLISMD EFRTMWKLFN 600
    AHYKAHIDDS QIDELASIVD FNKDGNIDFN EFLKAFYVVH KYDKPGTSLA 650
    Length:650
    Mass (Da):75,058
    Last modified:July 10, 2007 - v2
    Checksum:iF5B349BE006B60F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732450, AL974311 Genomic DNA. Translation: CAM17008.1.
    AL974311, AL732450 Genomic DNA. Translation: CAM19040.1.
    Y08234 Genomic DNA. Translation: CAA69403.1.
    CCDSiCCDS41200.1.
    RefSeqiNP_035277.1. NM_011147.1.
    UniGeneiMm.296779.

    Genome annotation databases

    EnsembliENSMUST00000071204; ENSMUSP00000071191; ENSMUSG00000062168.
    GeneIDi237178.
    KEGGimmu:237178.
    UCSCiuc009uto.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732450 , AL974311 Genomic DNA. Translation: CAM17008.1 .
    AL974311 , AL732450 Genomic DNA. Translation: CAM19040.1 .
    Y08234 Genomic DNA. Translation: CAA69403.1 .
    CCDSi CCDS41200.1.
    RefSeqi NP_035277.1. NM_011147.1.
    UniGenei Mm.296779.

    3D structure databases

    ProteinModelPortali O35655.
    SMRi O35655. Positions 72-638.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000071191.

    PTM databases

    PhosphoSitei O35655.

    Proteomic databases

    MaxQBi O35655.
    PRIDEi O35655.

    Protocols and materials databases

    DNASUi 237178.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000071204 ; ENSMUSP00000071191 ; ENSMUSG00000062168 .
    GeneIDi 237178.
    KEGGi mmu:237178.
    UCSCi uc009uto.2. mouse.

    Organism-specific databases

    CTDi 5475.
    MGIi MGI:1097157. Ppef1.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063173.
    HOGENOMi HOG000006820.
    HOVERGENi HBG008238.
    InParanoidi O35655.
    KOi K13807.
    OMAi MMNLRYG.
    OrthoDBi EOG7KH9J7.
    TreeFami TF313342.

    Enzyme and pathway databases

    Reactomei REACT_188266. Inactivation, recovery and regulation of the phototransduction cascade.

    Miscellaneous databases

    NextBioi 383242.
    PROi O35655.
    SOURCEi Search...

    Gene expression databases

    Bgeei O35655.
    Genevestigatori O35655.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    3.60.21.10. 2 hits.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000048. IQ_motif_EF-hand-BS.
    IPR029052. Metallo-depent_PP-like.
    IPR013235. PPP_dom.
    IPR012008. Ser/Thr-Pase_EF-hand_contain.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 1 hit.
    PF00149. Metallophos. 1 hit.
    PF08321. PPP5. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000912. PPEF. 1 hit.
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00054. EFh. 3 hits.
    SM00015. IQ. 1 hit.
    SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 2 hits.
    PROSITEi PS00018. EF_HAND_1. 3 hits.
    PS50222. EF_HAND_2. 3 hits.
    PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "A novel human serine-threonine phosphatase related to the Drosophila retinal degeneration C (rdgC) gene is selectively expressed in sensory neurons of neural crest origin."
      Montini E., Rugarli E.I., van de Vosse E., Andolfi G., Mariani M., Puca A.A., Consales G.G., den Dunnen J.T., Ballabio A., Franco B.
      Hum. Mol. Genet. 6:1137-1145(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-253.

    Entry informationi

    Entry nameiPPE1_MOUSE
    AccessioniPrimary (citable) accession number: O35655
    Secondary accession number(s): A2AIC6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3