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Protein

Serine/threonine-protein phosphatase with EF-hands 1

Gene

Ppef1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May have a role in the recovery or adaptation response of photoreceptors. May have a role in diverse sensory neurons and in development.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity
  • Mg2+By similarity

Enzyme regulationi

Activated by calcium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751Manganese 1By similarity
Metal bindingi177 – 1771Manganese 1By similarity
Metal bindingi204 – 2041Manganese 1By similarity
Metal bindingi204 – 2041Manganese 2By similarity
Metal bindingi236 – 2361Manganese 2By similarity
Active sitei237 – 2371Proton donorBy similarity
Metal bindingi288 – 2881Manganese 2By similarity
Metal bindingi404 – 4041Manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi497 – 508121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi580 – 591122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi620 – 631123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • dephosphorylation Source: GOC
  • detection of stimulus involved in sensory perception Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_311545. Inactivation, recovery and regulation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase with EF-hands 1 (EC:3.1.3.16)
Short name:
PPEF-1
Alternative name(s):
DRES10
Protein phosphatase with EF calcium-binding domain
Short name:
PPEF
Gene namesi
Name:Ppef1
Synonyms:Dres10, Ppef
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1097157. Ppef1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650Serine/threonine-protein phosphatase with EF-hands 1PRO_0000058900Add
BLAST

Proteomic databases

MaxQBiO35655.
PRIDEiO35655.

PTM databases

PhosphoSiteiO35655.

Expressioni

Tissue specificityi

In the embryo it is almost exclusively expressed in the peripheral nervous system, within sensory neurons of cranial and dorsal root ganglia. Otherwise found in fetal inner ear and a small group of neurons in the midbrain/pons junction.

Developmental stagei

Up-regulated at E12.3 in dorsal root ganglia (DRG) and in some sensory cranial ganglia. A slightly decreased expression could still be detected in sensory ganglia at E16.5. It is not known if expression in sensory neurons persists in adult life.

Gene expression databases

BgeeiO35655.
ExpressionAtlasiO35655. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071191.

Structurei

3D structure databases

ProteinModelPortaliO35655.
SMRiO35655. Positions 72-639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 4530IQAdd
BLAST
Domaini484 – 51936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini567 – 60236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini607 – 64236EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 456333CatalyticBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family.Curated
Contains 3 EF-hand domains.PROSITE-ProRule annotation
Contains 1 IQ domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063173.
HOGENOMiHOG000006820.
HOVERGENiHBG008238.
InParanoidiO35655.
KOiK13807.
OMAiMMNLRYG.
OrthoDBiEOG7KH9J7.
TreeFamiTF313342.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PIRSFiPIRSF000912. PPEF. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35655-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCGTSSKKG NKSKKVIKAA LVIQNWYRRY RARLRVRQHY ALAIFQSIEY
60 70 80 90 100
SDEQGQMQLS SFFSFMLENY TKTNNEDSAL VTRIFDNTRR ESQIKDRDDF
110 120 130 140 150
LGLIEVPDSY DGPRLQFPLT FTDIHILLQA FKQQQILHAH YVLEVLFEAR
160 170 180 190 200
KVLKQMPNFS HVKTFPAKEI TICGDLHGKL DDLMLIFYKN GLPSENNPYV
210 220 230 240 250
FNGDFVDRGN NSMEILMILL VCFLVYPSDL HLNRGNHEDF MMNLRYGFTK
260 270 280 290 300
EILQKYKLHG RKILQVLEEV YTWLPIGTII DNEILVIHGG ISESTDLNTL
310 320 330 340 350
HQLQRNKMKS VLMPPVLGNQ ETGEKRNKSA SNYVEPRKVE PDKTPSEDLT
360 370 380 390 400
KQEWEQIVDI LWSDPRGKKG CYPNTSRGGG CYFGPDVTSK VLSKNQLKML
410 420 430 440 450
IRSHECKPDG YEVSHDGKVI TVFSASNYYE EGSNRGAYIR LSYGTMPQFF
460 470 480 490 500
QYQVTSTSCL NPLHQRMNAM ESSAFKILKE KMISRKTDLI NAFELRDHSR
510 520 530 540 550
SGRISLAEWA FSMENILGLN LPWRSLSSHL VTIDSSGSVD YMSSFDDIRI
560 570 580 590 600
EKPTKDMKSN LTETMYRYRS DLKIIFNIID SDQSGLISMD EFRTMWKLFN
610 620 630 640 650
AHYKAHIDDS QIDELASIVD FNKDGNIDFN EFLKAFYVVH KYDKPGTSLA
Length:650
Mass (Da):75,058
Last modified:July 10, 2007 - v2
Checksum:iF5B349BE006B60F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732450, AL974311 Genomic DNA. Translation: CAM17008.1.
AL974311, AL732450 Genomic DNA. Translation: CAM19040.1.
Y08234 Genomic DNA. Translation: CAA69403.1.
CCDSiCCDS41200.1.
RefSeqiNP_035277.1. NM_011147.1.
UniGeneiMm.296779.

Genome annotation databases

EnsembliENSMUST00000071204; ENSMUSP00000071191; ENSMUSG00000062168.
GeneIDi237178.
KEGGimmu:237178.
UCSCiuc009uto.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732450, AL974311 Genomic DNA. Translation: CAM17008.1.
AL974311, AL732450 Genomic DNA. Translation: CAM19040.1.
Y08234 Genomic DNA. Translation: CAA69403.1.
CCDSiCCDS41200.1.
RefSeqiNP_035277.1. NM_011147.1.
UniGeneiMm.296779.

3D structure databases

ProteinModelPortaliO35655.
SMRiO35655. Positions 72-639.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071191.

PTM databases

PhosphoSiteiO35655.

Proteomic databases

MaxQBiO35655.
PRIDEiO35655.

Protocols and materials databases

DNASUi237178.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071204; ENSMUSP00000071191; ENSMUSG00000062168.
GeneIDi237178.
KEGGimmu:237178.
UCSCiuc009uto.2. mouse.

Organism-specific databases

CTDi5475.
MGIiMGI:1097157. Ppef1.

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063173.
HOGENOMiHOG000006820.
HOVERGENiHBG008238.
InParanoidiO35655.
KOiK13807.
OMAiMMNLRYG.
OrthoDBiEOG7KH9J7.
TreeFamiTF313342.

Enzyme and pathway databases

ReactomeiREACT_311545. Inactivation, recovery and regulation of the phototransduction cascade.

Miscellaneous databases

NextBioi383242.
PROiO35655.
SOURCEiSearch...

Gene expression databases

BgeeiO35655.
ExpressionAtlasiO35655. baseline and differential.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000048. IQ_motif_EF-hand-BS.
IPR029052. Metallo-depent_PP-like.
IPR013235. PPP_dom.
IPR012008. Ser/Thr-Pase_EF-hand_contain.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF13499. EF-hand_7. 1 hit.
PF00149. Metallophos. 1 hit.
PF08321. PPP5. 1 hit.
[Graphical view]
PIRSFiPIRSF000912. PPEF. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00054. EFh. 3 hits.
SM00015. IQ. 1 hit.
SM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "A novel human serine-threonine phosphatase related to the Drosophila retinal degeneration C (rdgC) gene is selectively expressed in sensory neurons of neural crest origin."
    Montini E., Rugarli E.I., van de Vosse E., Andolfi G., Mariani M., Puca A.A., Consales G.G., den Dunnen J.T., Ballabio A., Franco B.
    Hum. Mol. Genet. 6:1137-1145(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-253.

Entry informationi

Entry nameiPPE1_MOUSE
AccessioniPrimary (citable) accession number: O35655
Secondary accession number(s): A2AIC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: July 10, 2007
Last modified: June 24, 2015
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.