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Protein

DNA polymerase delta subunit 2

Gene

Pold2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The function of the small subunit is not yet clear.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA-dependent DNA replication Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

ReactomeiREACT_276436. Processive synthesis on the lagging strand.
REACT_278371. Processive synthesis on the C-strand of the telomere.
REACT_278538. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_283766. Polymerase switching.
REACT_290560. Leading Strand Synthesis.
REACT_303193. Removal of the Flap Intermediate.
REACT_304724. Telomere C-strand (Lagging Strand) Synthesis.
REACT_320650. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
REACT_326745. Removal of DNA patch containing abasic residue.
REACT_329955. Removal of the Flap Intermediate from the C-strand.
REACT_330619. Polymerase switching on the C-strand of the telomere.
REACT_331202. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
REACT_337003. Resolution of AP sites via the multiple-nucleotide patch replacement pathway.
REACT_342741. Repair synthesis of patch ~27-30 bases long by DNA polymerase.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase delta subunit 2 (EC:2.7.7.7)
Alternative name(s):
DNA polymerase delta subunit p50
Gene namesi
Name:Pold2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1097163. Pold2.

Subcellular locationi

GO - Cellular componenti

  1. delta DNA polymerase complex Source: Ensembl
  2. nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469DNA polymerase delta subunit 2PRO_0000096167Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO35654.
PaxDbiO35654.
PRIDEiO35654.

PTM databases

PhosphoSiteiO35654.

Expressioni

Gene expression databases

BgeeiO35654.
ExpressionAtlasiO35654. baseline and differential.
GenevestigatoriO35654.

Interactioni

Subunit structurei

Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. Interacts with KCTD13/POLDIP1, POLDIP2, POLDIP3. Interacts with WRNIP1 and KCTD10. Interacts with POLD4 (By similarity).By similarity

Protein-protein interaction databases

BioGridi202291. 1 interaction.
IntActiO35654. 2 interactions.
MINTiMINT-1565891.
STRINGi10090.ENSMUSP00000099986.

Structurei

3D structure databases

ProteinModelPortaliO35654.
SMRiO35654. Positions 1-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1311.
GeneTreeiENSGT00390000006780.
HOGENOMiHOG000189057.
HOVERGENiHBG051396.
InParanoidiO35654.
KOiK02328.
OMAiTRLIQMR.
OrthoDBiEOG7RZ5PQ.
TreeFamiTF101073.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR024826. DNA_pol_delta/II_ssu.
[Graphical view]
PANTHERiPTHR10416. PTHR10416. 1 hit.
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSEQAAQRA HTLLAPPSAS NATFARVPVA TYTNSSQPFR LGERSFNRQY
60 70 80 90 100
AHIYATRLIQ MRPFLVSRAQ QHWGSRVEVK KLCELQPGEQ CCVVGTLFKA
110 120 130 140 150
MSLQPSILRE ISEEHNLVPQ PPRSKYIHPD DELVLEDELQ RIKLKGTIDV
160 170 180 190 200
SKLVTGTVLA VLGSAKDDGR FQVEDHCFAD LAPQKPVPPL DTDRFVLLVS
210 220 230 240 250
GLGLGGGGGE SLLGTQLLVD VVTGQLGDEG EQCSAAHVSR VILAGNLLSH
260 270 280 290 300
NTQSRDSINK AKYLTKKTQA ASVEAVKMLD EILLQLSASV PVDVMPGEFD
310 320 330 340 350
PTNYTLPQQP LHPCMFPLAT AYSTLQLVTN PYQATIDGVR FLGTSGQNVS
360 370 380 390 400
DIFRYSSMED HLEILEWTLR VRHISPTAPD TLGCYPFYKT DPFIFPECPH
410 420 430 440 450
VYFCGNTPSF GSKIIRGPED QVVLLVAVPD FSSTQTACLV NLRSLACQPI
460
SFAGFGAEQE DLEGLGLGP
Length:469
Mass (Da):51,355
Last modified:July 26, 2011 - v2
Checksum:i1203465743C80900
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671S → T in CAA96567 (PubMed:9286699).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72486 mRNA. Translation: CAA96567.1.
AL627069 Genomic DNA. Translation: CAI25442.1.
CH466574 Genomic DNA. Translation: EDL40563.1.
BC003342 mRNA. Translation: AAH03342.1.
CCDSiCCDS24407.1.
RefSeqiNP_032920.2. NM_008894.2.
XP_006514650.1. XM_006514587.2.
UniGeneiMm.35788.

Genome annotation databases

EnsembliENSMUST00000102922; ENSMUSP00000099986; ENSMUSG00000020471.
GeneIDi18972.
KEGGimmu:18972.
UCSCiuc007hxk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z72486 mRNA. Translation: CAA96567.1.
AL627069 Genomic DNA. Translation: CAI25442.1.
CH466574 Genomic DNA. Translation: EDL40563.1.
BC003342 mRNA. Translation: AAH03342.1.
CCDSiCCDS24407.1.
RefSeqiNP_032920.2. NM_008894.2.
XP_006514650.1. XM_006514587.2.
UniGeneiMm.35788.

3D structure databases

ProteinModelPortaliO35654.
SMRiO35654. Positions 1-458.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202291. 1 interaction.
IntActiO35654. 2 interactions.
MINTiMINT-1565891.
STRINGi10090.ENSMUSP00000099986.

PTM databases

PhosphoSiteiO35654.

Proteomic databases

MaxQBiO35654.
PaxDbiO35654.
PRIDEiO35654.

Protocols and materials databases

DNASUi18972.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102922; ENSMUSP00000099986; ENSMUSG00000020471.
GeneIDi18972.
KEGGimmu:18972.
UCSCiuc007hxk.2. mouse.

Organism-specific databases

CTDi5425.
MGIiMGI:1097163. Pold2.

Phylogenomic databases

eggNOGiCOG1311.
GeneTreeiENSGT00390000006780.
HOGENOMiHOG000189057.
HOVERGENiHBG051396.
InParanoidiO35654.
KOiK02328.
OMAiTRLIQMR.
OrthoDBiEOG7RZ5PQ.
TreeFamiTF101073.

Enzyme and pathway databases

ReactomeiREACT_276436. Processive synthesis on the lagging strand.
REACT_278371. Processive synthesis on the C-strand of the telomere.
REACT_278538. Repair synthesis for gap-filling by DNA polymerase in TC-NER.
REACT_283766. Polymerase switching.
REACT_290560. Leading Strand Synthesis.
REACT_303193. Removal of the Flap Intermediate.
REACT_304724. Telomere C-strand (Lagging Strand) Synthesis.
REACT_320650. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).
REACT_326745. Removal of DNA patch containing abasic residue.
REACT_329955. Removal of the Flap Intermediate from the C-strand.
REACT_330619. Polymerase switching on the C-strand of the telomere.
REACT_331202. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta).
REACT_337003. Resolution of AP sites via the multiple-nucleotide patch replacement pathway.
REACT_342741. Repair synthesis of patch ~27-30 bases long by DNA polymerase.

Miscellaneous databases

ChiTaRSiPold2. mouse.
NextBioi295324.
PROiO35654.
SOURCEiSearch...

Gene expression databases

BgeeiO35654.
ExpressionAtlasiO35654. baseline and differential.
GenevestigatoriO35654.

Family and domain databases

InterProiIPR007185. DNA_pol_alpha/epsilon_bsu.
IPR024826. DNA_pol_delta/II_ssu.
[Graphical view]
PANTHERiPTHR10416. PTHR10416. 1 hit.
PfamiPF04042. DNA_pol_E_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, chromosomal localization, and interspecies interaction of mouse DNA polymerase delta small subunit (PolD2)."
    Hindges R., Huebscher U.
    Genomics 44:45-51(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NMRI.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiDPOD2_MOUSE
AccessioniPrimary (citable) accession number: O35654
Secondary accession number(s): Q99J61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 1998
Last sequence update: July 26, 2011
Last modified: March 31, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.