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Protein

Calpain-6

Gene

Capn6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-stabilizing protein that may be involved in the regulation of microtubule dynamics and cytoskeletal organization. May act as a regulator of RAC1 activity through interaction with ARHGEF2 to control lamellipodial formation and cell mobility. Does not seem to have protease activity as it has lost the active site residues.2 Publications

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: GO_Central
  • microtubule binding Source: UniProtKB

GO - Biological processi

  • microtubule bundle formation Source: UniProtKB
  • proteolysis Source: GO_Central
  • regulation of cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BRENDAi3.4.22.B26. 3474.

Protein family/group databases

MEROPSiC02.971.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-6
Gene namesi
Name:Capn6
Synonyms:Capa6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1100850. Capn6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • microtubule Source: UniProtKB
  • perinuclear region of cytoplasm Source: MGI
  • spindle microtubule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 641641Calpain-6PRO_0000207718Add
BLAST

Proteomic databases

MaxQBiO35646.
PaxDbiO35646.
PeptideAtlasiO35646.
PRIDEiO35646.

PTM databases

iPTMnetiO35646.
PhosphoSiteiO35646.

Expressioni

Developmental stagei

At E10.5 expressed in the mandibular arches, heart and limb buds.1 Publication

Inductioni

Up-regulated during the osteoclasogenic process. Inhibited by dexamethaxone during the osteoclasogenic process.1 Publication

Gene expression databases

BgeeiENSMUSG00000067276.
CleanExiMM_CAPN6.
GenevisibleiO35646. MM.

Interactioni

Subunit structurei

Interacts (via domain III) with microtubules. Interacts (via domain II) with ARHGEF2 (via the N-terminal zinc finger).2 Publications

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

BioGridi198475. 1 interaction.
IntActiO35646. 1 interaction.
MINTiMINT-4089799.
STRINGi10090.ENSMUSP00000084573.

Structurei

3D structure databases

ProteinModelPortaliO35646.
SMRiO35646. Positions 8-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 343318Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini518 – 620103C2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni344 – 495152Domain IIIAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 C2 domain.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232036.
HOVERGENiHBG001095.
InParanoidiO35646.
KOiK08575.
OMAiDICDDPH.
OrthoDBiEOG091G030A.
TreeFamiTF314748.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029540. CAPN6.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF281. PTHR10183:SF281. 1 hit.
PfamiPF00168. C2. 1 hit.
PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00239. C2. 1 hit.
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O35646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPPLKLFKN QKYQELKQEC MKDGRLFCDP TFLPENDSLF FNRLLPGKVV
60 70 80 90 100
WKRPQDISDD PHLIVGNISN HQLIQGRLGN KAMISAFSCL AVQESHWTKA
110 120 130 140 150
IPNHKDQEWD PRKPEKYAGI FHFRFWHFGE WTEVVIDDLL PTINGDLVFS
160 170 180 190 200
FSTSMNEFWN ALLEKAYAKL LGCYEALDGL TITDIIMDFT GTLAEIIDMQ
210 220 230 240 250
KGRYTDLVEE KYKLFGELYK TFTKGGLICC SIESPSQEEQ EVETDWGLLK
260 270 280 290 300
GYTYTMTDIR KLRLGERLVE VFSTEKLYMV RLRNPLGRQE WSGPWSEISE
310 320 330 340 350
EWQQLTVTDR KNLGLVMSDD GEFWMSLEDF CHNFHKLNVC RNVNNPVFGR
360 370 380 390 400
KELESVVGCW TVDDDPLMNR SGGCYNNRDT FLQNPQYIFT VPEDGHKVIM
410 420 430 440 450
SLQQKDLRTY RRMGRPDNYI IGFELFKVEM NRRFRLHHLY IQERAGTSTY
460 470 480 490 500
IDTRTVFLSK YLKKGSYVLV PTMFQHGRTS EFLLRIFSEV PVQLRELTLD
510 520 530 540 550
MPKMSCWNLA RGYPKVVTQI TVHSAEGLEK KYANETVNPY LIIKCGKEEV
560 570 580 590 600
RSPVQKNTVH AIFDTQAIFY RRTTDIPIII QVWNSRKFCD QFLGQVTLDA
610 620 630 640
DPSDCRDLKS LYLRKKGGPT AKVKQGHISF KVISSDDLTE L
Length:641
Mass (Da):74,543
Last modified:July 27, 2011 - v2
Checksum:i3F7D8DBC615B57A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti568 – 5681I → V in CAA73161 (PubMed:9503024).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12582 mRNA. Translation: CAA73161.1.
AK145116 mRNA. Translation: BAE26243.1.
BX530055 Genomic DNA. Translation: CAM25088.1.
CCDSiCCDS30455.1.
RefSeqiNP_031629.3. NM_007603.3.
UniGeneiMm.30290.

Genome annotation databases

EnsembliENSMUST00000087316; ENSMUSP00000084573; ENSMUSG00000067276.
GeneIDi12338.
KEGGimmu:12338.
UCSCiuc009umk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12582 mRNA. Translation: CAA73161.1.
AK145116 mRNA. Translation: BAE26243.1.
BX530055 Genomic DNA. Translation: CAM25088.1.
CCDSiCCDS30455.1.
RefSeqiNP_031629.3. NM_007603.3.
UniGeneiMm.30290.

3D structure databases

ProteinModelPortaliO35646.
SMRiO35646. Positions 8-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198475. 1 interaction.
IntActiO35646. 1 interaction.
MINTiMINT-4089799.
STRINGi10090.ENSMUSP00000084573.

Protein family/group databases

MEROPSiC02.971.

PTM databases

iPTMnetiO35646.
PhosphoSiteiO35646.

Proteomic databases

MaxQBiO35646.
PaxDbiO35646.
PeptideAtlasiO35646.
PRIDEiO35646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087316; ENSMUSP00000084573; ENSMUSG00000067276.
GeneIDi12338.
KEGGimmu:12338.
UCSCiuc009umk.2. mouse.

Organism-specific databases

CTDi827.
MGIiMGI:1100850. Capn6.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
GeneTreeiENSGT00760000118971.
HOGENOMiHOG000232036.
HOVERGENiHBG001095.
InParanoidiO35646.
KOiK08575.
OMAiDICDDPH.
OrthoDBiEOG091G030A.
TreeFamiTF314748.

Enzyme and pathway databases

BRENDAi3.4.22.B26. 3474.

Miscellaneous databases

PROiO35646.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000067276.
CleanExiMM_CAPN6.
GenevisibleiO35646. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029540. CAPN6.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF281. PTHR10183:SF281. 1 hit.
PfamiPF00168. C2. 1 hit.
PF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00239. C2. 1 hit.
SM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN6_MOUSE
AccessioniPrimary (citable) accession number: O35646
Secondary accession number(s): Q3UM55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.