Reviewed, UniProtKB/Swiss-Prot O35643 (AP1B1_MOUSE)

Names and origin

Protein names

Recommended name:
AP-1 complex subunit beta-1

Alternative name(s):
Adapter-related protein complex 1 subunit beta-1
Adaptor protein complex AP-1 subunit beta-1
Beta-adaptin 1
Beta-1-adaptin
Golgi adaptor HA1/AP1 adaptin beta subunit
Clathrin assembly protein complex 1 beta large chain

Gene names

Name:	Ap1b1
Synonyms:	Adtb1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	943 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatus By similarity. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex By similarity.
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
Biological process	Endocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
PTM	Acetylation Nitration Phosphoprotein
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Traceable author statement. Source: MGI
Cellular component	clathrin adaptor complex Inferred from electronic annotation. Source: InterPro clathrin coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network Traceable author statement. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 943	943	AP-1 complex subunit beta-1		PRO_0000193739
Regions
	Compositional bias	576 – 722	147	Pro-rich (stalk region)
Amino acid modifications
	Modified residue	318	1	N6-acetyllysine By similarity
	Modified residue	574	1	Nitrated tyrosine Ref.3
	Modified residue	894	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	227	1	D → A in AAH08513. Ref.2
	Sequence conflict	288	1	L → P in AAH08513. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O35643-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7A67869C1CAAF973	FASTA	943	103,979
	10 20 30 40 50 60 MTDSKYFTTT KKGEIFELKA ELNSDKKEKK KEAVKKVIAS MTVGKDVSAL FPDVVNCMQT 70 80 90 100 110 120 DNLELKKLVY LYLMNYAKSQ PDMAIMAVNT FVKDCEDPNP LIRALAVRTM GCIRVDKITE 130 140 150 160 170 180 YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD INAQLVEDQG FLDTLKDLIS DSNPMVVANA 190 200 210 220 230 240 VAALSEIAES HPSSNLLDLN PQSINKLLTA LNECTEWGQI FILDCLDNYM PKDDREAQSI 250 260 270 280 290 300 CERVTPRLSH ANSAVVLSAV KVLMKFMEML SKDLDYYATL LKKLAPPLVT LLSAEPELQY 310 320 330 340 350 360 VALRNINLIV QKRPEILKHE MKVFFVKYND PIYVKLEKLD IMIRLASQAN IAQVLAELKE 370 380 390 400 410 420 YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ TKVNYVVQEA IVVIKDIFRK 430 440 450 460 470 480 YPNKYESVIA TLCENLDSLD EPEARAAMIW IVGEYAERID NADELLESFL EGFHDESTQV 490 500 510 520 530 540 QLQLLTAIVK LFLKKPTETQ ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVAAKEV 550 560 570 580 590 600 VLAEKPLISE ETDLIEPTLL DELICYIGTL ASVYHKPPNA FVEGGRGVVH KSLPPRTASS 610 620 630 640 650 660 ESTESPETAP AGAPAGDQPD VIPAQGDLLG DLLNLDLGPP VSGPPLAASS VQMGAVDLLG 670 680 690 700 710 720 GGLDSLIGDS NFGAPSASVA AAPAPARLGA PISSGLSDLF DLTSGVGTLS GSYVAPKAVW 730 740 750 760 770 780 LPAMKAKGLE ISGTFTRQAG SISMDLQLTN KALQVMTDFA IQFNRNSFGL APAAPLQVHV 790 800 810 820 830 840 PLSPNQTVEI SLPLNTVGSV LKMEPLNNLQ VAVKNNIDVF YFSTLYPLHV LFVEDGKMDR 850 860 870 880 890 900 QMFLATWKDI ANENEAQFQI RDCPLNTEAA SNKLQSSNIF TVAKRNVEGQ DMLYQSLKLT 910 920 930 940 NGIWVLAELR IQPGNPSFTL SLKCRAPEVS QHVYQAYETI LKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the mouse beta-prime adaptin gene; cDNA sequence, genomic structure, and chromosomal localization." Guilbaud C., Peyrard M., Fransson I., Clifton S.W., Roe B.A., Carter N.P., Dumanski J.P. Mamm. Genome 8:651-656(1997) [PubMed: 9271666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Embryonic brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[3]	"Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease." Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C., Bigelow D.J. Biochemistry 45:8009-8022(2006) [PubMed: 16800626] [Abstract] Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-574, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07919 mRNA. Translation: CAA69224.1. BC008513 mRNA. Translation: AAH08513.1.
IPI	IPI00648683.
UniGene	Mm.274816
3D structure databases
SMR	O35643. Positions 2-583, 711-943.
ModBase	Search...
Protein-protein interaction databases
STRING	O35643.
PTM databases
PhosphoSite	O35643.
Proteomic databases
PRIDE	O35643.
Genome annotation databases
Ensembl	ENSMUST00000009234; ENSMUSP00000009234; ENSMUSG00000009090; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:1096368. Ap1b1.
Phylogenomic databases
eggNOG	roNOG10896.
HOVERGEN	HBG050515.
PhylomeDB	O35643.
Gene expression databases
ArrayExpress	O35643.
Bgee	O35643.
CleanEx	MM_AP1B1.
Genevestigator	O35643.
Family and domain databases
InterPro	IPR016342. AP_complex_bsu. IPR016024. ARM-type_fold. IPR012295. b_Adaptin_TBP_C. IPR009028. Calthrin/coatomer_app_sub_C. IPR002553. Clathrin/coatomer_adapt-like_N. IPR013041. Clathrin/coatomer_app_Ig-like. IPR008152. Clathrin_a/b/g-adaptin_app_Ig. IPR013037. Clathrin_b-adaptin_app_Ig-like. IPR015151. Clathrin_b-adaptin_app_sub_C. [Graphical view]
Gene3D	G3DSA:3.30.310.10. b_Adaptin_TBP_C. 1 hit. G3DSA:2.60.40.1150. Clathrin_b-adaptin_app_Ig-like. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. PF02883. Alpha_adaptinC2. 1 hit. PF09066. B2-adapt-app_C. 1 hit. [Graphical view]
PIRSF	PIRSF002291. AP_complex_beta. 1 hit.
SMART	SM00809. Alpha_adaptinC2. 1 hit. [Graphical view]
SUPFAM	SSF55711. AP2_adap_app. 1 hit. SSF48371. ARM-type_fold. 1 hit. SSF49348. Clath_adapt. 1 hit.
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

AP1B1_MOUSE

Accession

Primary (citable) accession number: O35643
Secondary accession number(s): Q922E2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	January 1, 1998
Last modified:	March 2, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)