Skip Header

Contribute Send feedback
Read comments (?) or add your own

O35643 (AP1B1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit beta-1
Alternative name(s):
Adapter-related protein complex 1 subunit beta-1
Adaptor protein complex AP-1 subunit beta-1
Beta-1-adaptin
Beta-adaptin 1
Clathrin assembly protein complex 1 beta large chain
Golgi adaptor HA1/AP1 adaptin beta subunit
Gene names
Name:Ap1b1
Synonyms:Adtb1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length943 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).

Subcellular location

Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Golgi apparatus By similarity. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex By similarity.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 943943AP-1 complex subunit beta-1
PRO_0000193739

Regions

Compositional bias576 – 722147Pro-rich (stalk region)

Amino acid modifications

Modified residue3181N6-acetyllysine By similarity
Modified residue5741Nitrated tyrosine Ref.5
Modified residue8941Phosphotyrosine By similarity

Experimental info

Sequence conflict2271A → D in CAA69224. Ref.1
Sequence conflict2881L → P in AAH08513. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O35643 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 5DD1951EAAC20225

FASTA943103,935
        10         20         30         40         50         60 
MTDSKYFTTT KKGEIFELKA ELNSDKKEKK KEAVKKVIAS MTVGKDVSAL FPDVVNCMQT 

        70         80         90        100        110        120 
DNLELKKLVY LYLMNYAKSQ PDMAIMAVNT FVKDCEDPNP LIRALAVRTM GCIRVDKITE 

       130        140        150        160        170        180 
YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD INAQLVEDQG FLDTLKDLIS DSNPMVVANA 

       190        200        210        220        230        240 
VAALSEIAES HPSSNLLDLN PQSINKLLTA LNECTEWGQI FILDCLANYM PKDDREAQSI 

       250        260        270        280        290        300 
CERVTPRLSH ANSAVVLSAV KVLMKFMEML SKDLDYYATL LKKLAPPLVT LLSAEPELQY 

       310        320        330        340        350        360 
VALRNINLIV QKRPEILKHE MKVFFVKYND PIYVKLEKLD IMIRLASQAN IAQVLAELKE 

       370        380        390        400        410        420 
YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ TKVNYVVQEA IVVIKDIFRK 

       430        440        450        460        470        480 
YPNKYESVIA TLCENLDSLD EPEARAAMIW IVGEYAERID NADELLESFL EGFHDESTQV 

       490        500        510        520        530        540 
QLQLLTAIVK LFLKKPTETQ ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVAAKEV 

       550        560        570        580        590        600 
VLAEKPLISE ETDLIEPTLL DELICYIGTL ASVYHKPPNA FVEGGRGVVH KSLPPRTASS 

       610        620        630        640        650        660 
ESTESPETAP AGAPAGDQPD VIPAQGDLLG DLLNLDLGPP VSGPPLAASS VQMGAVDLLG 

       670        680        690        700        710        720 
GGLDSLIGDS NFGAPSASVA AAPAPARLGA PISSGLSDLF DLTSGVGTLS GSYVAPKAVW 

       730        740        750        760        770        780 
LPAMKAKGLE ISGTFTRQAG SISMDLQLTN KALQVMTDFA IQFNRNSFGL APAAPLQVHV 

       790        800        810        820        830        840 
PLSPNQTVEI SLPLNTVGSV LKMEPLNNLQ VAVKNNIDVF YFSTLYPLHV LFVEDGKMDR 

       850        860        870        880        890        900 
QMFLATWKDI ANENEAQFQI RDCPLNTEAA SNKLQSSNIF TVAKRNVEGQ DMLYQSLKLT 

       910        920        930        940 
NGIWVLAELR IQPGNPSFTL SLKCRAPEVS QHVYQAYETI LKN

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the mouse beta-prime adaptin gene; cDNA sequence, genomic structure, and chromosomal localization."
Guilbaud C., Peyrard M., Fransson I., Clifton S.W., Roe B.A., Carter N.P., Dumanski J.P.
Mamm. Genome 8:651-656(1997) [PubMed: 9271666] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Embryonic brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary gland.
[5]"Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease."
Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C., Bigelow D.J.
Biochemistry 45:8009-8022(2006) [PubMed: 16800626] [Abstract]
Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-574, MASS SPECTROMETRY.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Y07919 mRNA. Translation: CAA69224.1.
AK159273 mRNA. Translation: BAE34952.1.
CH466574 Genomic DNA. Translation: EDL40509.1.
BC008513 mRNA. Translation: AAH08513.1.
IPIIPI00346965.
RefSeqNP_031480.2. NM_007454.3.
UniGeneMm.274816.

3D structure databases

ProteinModelPortalO35643.
SMRO35643. Positions 2-583, 711-943.
ModBaseSearch...

Protein-protein interaction databases

IntActO35643. 4 interactions.
STRINGO35643.

PTM databases

PhosphoSiteO35643.

Proteomic databases

PRIDEO35643.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000009234; ENSMUSP00000009234; ENSMUSG00000009090.
GeneID11764.
KEGGmmu:11764.

Organism-specific databases

CTD162.
MGIMGI:1096368. Ap1b1.

Phylogenomic databases

eggNOGroNOG10896.
HOVERGENHBG050515.
PhylomeDBO35643.

Gene expression databases

ArrayExpressO35643.
BgeeO35643.
CleanExMM_AP1B1.
GenevestigatorO35643.

Family and domain databases

InterProIPR016342. AP_complex_bsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013037. Clathrin_b-adaptin_app_Ig-like.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
Gene3DG3DSA:1.25.10.10. ARM-like. 1 hit.
G3DSA:2.60.40.1150. Clathrin_b-adaptin_app_Ig-like. 1 hit.
KOK12392.
PfamPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFPIRSF002291. AP_complex_beta. 1 hit.
SMARTSM00809. Alpha_adaptinC2. 1 hit.
SM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMSSF55711. AP2_adap_app. 1 hit.
SSF48371. ARM-type_fold. 1 hit.
SSF49348. Clath_adapt. 1 hit.
ProtoNetSearch...

Other

SOURCESearch...

Entry information

Entry nameAP1B1_MOUSE
AccessionPrimary (citable) accession number: O35643
Secondary accession number(s): Q3TXG4, Q922E2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: July 27, 2011
Last modified: January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents